Ambient temperature and kidney function in primary care patients.

INTRODUCTION: Exposure to high ambient temperatures is associated with a risk of acute kidney injury. However, evidence comes from emergency departments or extreme weather exposures. It is unclear whether temperature-related adverse kidney outcomes can also be detected at a community level in a temperate climate zone. METHODS: In a 9.5-year retrospective cohort study we correlated estimated glomerular filtration rate (eGFR) values of Swiss adult primary care patients from the FIRE cohort (Family medicine Research using Electronic medical records) with same-day maximum local ambient temperature data. We investigated 5 temperature groups (< 15 °C, 15-19 °C, 20-24 °C, 25-29 °C and ≥ 30 °C) as well as possible interactions for patients with increased kidney vulnerability (chronic heart failure, diabetes, chronic kidney disease, therapy with renin-angiotensin-aldosterone-system (RAAS) inhibitors, diuretics or non-steroidal anti-inflammatory drugs). RESULTS: We included 18,000 primary care patients who altogether provided 132,176 creatinine measurements. In the unadjusted analysis, higher ambient temperatures were associated with lower eGFR across all age and vulnerability groups. In the adjusted models, we did not find a consistent association.The highest ambient temperature differences (> 25 or > 30 versus < 15 °C) were associated with marginally reduced kidney function only in patients with ≥ 3 risk factors for kidney vulnerability, with a maximum estimated glomerular filtration rate reduction of -2.9 ml/min/1.73m2 (SE 1.0), P 0.003. DISCUSSION: In a large primary care cohort from a temperate climate zone, we did not find an association between ambient temperatures and kidney function. A marginal inverse association in highly vulnerable patients is of unclear clinical relevance.

Citizen science approaches for water quality measurements.

Citizen science has become a widely used approach in water quality studies. Although there are literature reviews about citizen science and water quality assessments, an overview of the most commonly used methods and their strengths and weaknesses is still lacking. Therefore, we reviewed the scientific literature on citizen science for surface water quality assessments and examined the methods and strategies used by the 72 studies that fulfilled our search criteria. Special attention was given to the parameters monitored, the monitoring tools, and the spatial and temporal resolution of the data collected in these studies. In addition, we discuss the advantages and disadvantages of the different approaches used in water quality assessments and their potential to complement traditional hydrological monitoring and research.

Enhanced genome assembly and a new official gene set for Tribolium castaneum.

BACKGROUND: The red flour beetle Tribolium castaneum has emerged as an important model organism for the study of gene function in development and physiology, for ecological and evolutionary genomics, for pest control and a plethora of other topics. RNA interference (RNAi), transgenesis and genome editing are well established and the resources for genome-wide RNAi screening have become available in this model. All these techniques depend on a high quality genome assembly and precise gene models. However, the first version of the genome assembly was generated by Sanger sequencing, and with a small set of RNA sequence data limiting annotation quality. RESULTS: Here, we present an improved genome assembly (Tcas5.2) and an enhanced genome annotation resulting in a new official gene set (OGS3) for Tribolium castaneum, which significantly increase the quality of the genomic resources. By adding large-distance jumping library DNA sequencing to join scaffolds and fill small gaps, the gaps in the genome assembly were reduced and the N50 increased to 4753kbp. The precision of the gene models was enhanced by the use of a large body of RNA-Seq reads of different life history stages and tissue types, leading to the discovery of 1452 novel gene sequences. We also added new features such as alternative splicing, well defined UTRs and microRNA target predictions. For quality control, 399 gene models were evaluated by manual inspection. The current gene set was submitted to Genbank and accepted as a RefSeq genome by NCBI. CONCLUSIONS: The new genome assembly (Tcas5.2) and the official gene set (OGS3) provide enhanced genomic resources for genetic work in Tribolium castaneum. The much improved information on transcription start sites supports transgenic and gene editing approaches. Further, novel types of information such as splice variants and microRNA target genes open additional possibilities for analysis.

The CrowdWater game: A playful way to improve the accuracy of crowdsourced water level class data.

Data quality control is important for any data collection program, especially in citizen science projects, where it is more likely that errors occur due to the human factor. Ideally, data quality control in citizen science projects is also crowdsourced so that it can handle large amounts of data. Here we present the CrowdWater game as a gamified method to check crowdsourced water level class data that are submitted by citizen scientists through the CrowdWater app. The app uses a virtual staff gauge approach, which means that a digital scale is added to the first picture taken at a site and this scale is used for water level class observations at different times. In the game, participants classify water levels based on the comparison of the new picture with the picture containing the virtual staff gauge. By March 2019, 153 people had played the CrowdWater game and 841 pictures were classified. The average water level for the game votes for the classified pictures was compared to the water level class submitted through the app to determine whether the game can improve the quality of the data submitted through the app. For about 70% of the classified pictures, the water level class was the same for the CrowdWater app and game. For a quarter of the classified pictures, there was disagreement between the value submitted through the app and the average game vote. Expert judgement suggests that for three quarters of these cases, the game based average value was correct. The initial results indicate that the CrowdWater game helps to identify erroneous water level class observations from the CrowdWater app and provides a useful approach for crowdsourced data quality control. This study thus demonstrates the potential of gamified approaches for data quality control in citizen science projects.

Bivariate analysis of floods in climate impact assessments.

Climate impact studies regarding floods usually focus on peak discharges and a bivariate assessment of peak discharges and hydrograph volumes is not commonly included. A joint consideration of peak discharges and hydrograph volumes, however, is crucial when assessing flood risks for current and future climate conditions. Here, we present a methodology to develop synthetic design hydrographs for future climate conditions that jointly consider peak discharges and hydrograph volumes. First, change factors are derived based on a regional climate model and are applied to observed precipitation and temperature time series. Second, the modified time series are fed into a calibrated hydrological model to simulate runoff time series for future conditions. Third, these time series are used to construct synthetic design hydrographs. The bivariate flood frequency analysis used in the construction of synthetic design hydrographs takes into account the dependence between peak discharges and hydrograph volumes, and represents the shape of the hydrograph. The latter is modeled using a probability density function while the dependence between the design variables peak discharge and hydrograph volume is modeled using a copula. We applied this approach to a set of eight mountainous catchments in Switzerland to construct catchment-specific and season-specific design hydrographs for a control and three scenario climates. Our work demonstrates that projected climate changes have an impact not only on peak discharges but also on hydrograph volumes and on hydrograph shapes both at an annual and at a seasonal scale. These changes are not necessarily proportional which implies that climate impact assessments on future floods should consider more flood characteristics than just flood peaks.

The Role of Prosocialness and Trust in the Consumption of Water as a Limited Resource.

This research analyzes the role of prosocialness and trust in the use of water as a limited resource under situations of competition or cooperation. For this purpose, 107 participants played the role of farmers and made decisions about irrigating their fields in the web-based multiplayer game Irrigania. Before the simulation exercise, participants' prosocialness and trust levels were evaluated and they were randomly assigned to an experimental condition (competition or cooperation). Repeated measures analysis, using the 10 fields and the experimental conditions as factors, showed that, in the cooperation condition, farmers and their villages used a less selfish strategy to cultivate their fields, which produced greater benefits. Under competition, benefits to farmers and their villages were reduced over time. Mediational analysis shows that the selfish irrigation strategy fully mediated the relationship between prosocialness and accumulated profits; prosocial individuals choose less selfish irrigation strategies and, in turn, accumulated more benefit. Moreover, moderation analysis shows that trust moderated the link between prosocialness and water use strategy by strengthening the negative effect of prosocialness on selection of selfish strategies. The implications of these results highlight the importance of promoting the necessary trust to develop prosocial strategies in collectives; therefore, the efficacy of interventions, such as the creation of cooperative educational contexts or organization of collective actions with groups affected by water scarcity, are discussed.

Forest harvest increases runoff most during low flows in two boreal streams.

To understand how forest harvest influences the aquatic environment, it is essential to determine the changes in the flow regime. This paper presents changes in the hydrological regime during the first 2 y after harvest in two catchments of the Balsjö Catchment Study in Sweden. The changes were judged relative to a reference catchment, calibrated during an 18-mo pretreatment period starting in September 2004. From August 2006 through March 2008, there was an average of 35% more runoff from the harvested catchments relative to the reference. The flow increased most during the growing seasons and at base flows (<1 mm d(-1); 58-99% increase), followed by dormant season and intermediate flows (30-43%). No significant changes were observed during the highest flows (over 5 mm d(-1)), except for the spring flood a few weeks after harvest, which was delayed and attenuated. Large relative changes in low flow may influence the ecosystem by altering the aquatic habitat.

An approach for including consideration of stream water dissolved organic carbon in long term forest planning.

The objective of this study was to add to a traditional forest planning model by incorporating some consideration of the effects of forestry on dissolved organic carbon (DOC) concentrations in streams over time. In a case study, for a watershed in northern Sweden, we present the best possible solution to a forest planning problem that maximizes the net present value (NPV) while the DOC concentration levels in the watershed are maintained below a defined threshold value. Results from the case study show that the decrease in NPV, when taking DOC into account, was considerable. However, the decrease in possible harvest volume was restricted in the case study area because the model moved harvesting activity from the first 20-year period to later periods to avoid high initial DOC concentrations. The model presented could be a useful tool for predicting the effect of forestry on DOC concentrations over time.

Dissolved inorganic carbon export across the soil/stream interface and its fate in a boreal headwater stream.

The aim of this investigation was to determine the lateral exportof dissolved inorganic carbon (DIC) from soils of a Swedish boreal forest to a first order stream and to estimate the partitioning of this DIC into CO2 evasion from the stream surface and the DIC pool exported down through the catchment by streamwater. The groundwater entering the stream was supersaturated with CO2 with values as high as 17 times equilibrium with the atmosphere. Up to 90% of the estimated daily soil DIC export to the stream was emitted to the atmosphere as CO2 within 200 m of the water entering the stream. The annual DIC export from the soil to the stream was estimated to be 3.2 (+/- 0.1) g C m(-2) yr(-1) (normalized to catchment size). Ninety percent of the variation in soil DIC export could be explained by the variation in groundwater discharge and the DIC concentrations per se, were of minor importance. A significant correlation (R(l) = 0.74, P < 0.01) between soil DIC export and CO2 emission from the stream surface suggests that emission dynamics were primarily driven by the export of terrestrial DIC and that in-stream processes were less important. Our results reveal that current budget estimates of lateral DIC export from soils to aquatic conduits need to be revised because they do not account for conditions prevailing in headwater streams. Any quantification of lateral stream C export and CO2 emissions from freshwater systems must include headwater streams as well as the lower parts of the aquatic conduit.

Spatial heterogeneity of the spring flood acid pulse in a boreal stream network.

Spatial and temporal patterns in streamwater acidity are ecologically important, but difficult to measure in parallel. Here we present the spatial distribution of streamwater chemistry relevant to acidity from 60 stream sites distributed throughout a 67 km2 boreal catchment, sampled during a period of winter baseflow (high pH) and during a spring flood episode (low pH). Sites were grouped based on pH level and pH change from winter baseflow to spring flood. The site attributes of each pH group were then assessed in terms of both stream chemistry and subcatchment landscape characteristics. Winter baseflow pH was high throughout most of the stream network (median pH 6.4), but during the spring flood episode stream sites experienced declines in pH ranging from 0-1.6 pH units, resulting in pH ranging from 4.3-6.3. Spring flood pH was highest in larger, lower altitude catchments underlain by fine sorted sediments, and lowest in small, higher altitude catchments with a mixture of peat wetlands and forested till. Wetland-dominated headwater catchments had low but stable pH, while the spring flood pH drop was largest in a group of catchments of intermediate size which contained well-developed coniferous forest and a moderate proportion of peat wetlands. There was a trend with distance downstream of higher pH, acid neutralizing capacity (ANC) and base cation concentrations together with lower dissolved organic carbon (DOC, strongly negatively correlated with pH). This apparent scale-dependence of stream chemistry could be explained by a number of environmental factors which vary predictably with altitude, catchment area and distance downstream--most notably, a shift in surficial sediment type from unsorted till and peat wetlands to fine sorted sediments at lower altitudes in this catchment. As a result of the combination of spatial heterogeneity in landscape characteristics and scale-related processes, boreal catchments like this one can be expected to experience high spatial variability both in terms of chemistry at any given point in time, and in the change experienced during high discharge episodes. Although chemistry patterns showed associations with landscape characteristics, considerable additional variability remained, suggesting that the modeling of dynamic stream chemistry from map parameters will continue to present a challenge.