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The present study was designed to evaluate the chemical composition, antioxidant, enzyme inhibition and cytotoxic properties of different extracts from aerial parts of V. diversifolium (family Scrophulariaceae), a plant that is native to Lebanon, Syria and Turkey. Six extracts, namely, hexane, dichloromethane (DCM), ethyl acetate (EtOAc), ethanol (EtOH), 70% EtOH, and water (aqueous) were prepared by maceration. The EtOH extract was predominated by the presence of rutin (4280.20 µg g-1) and p-coumaric acid (3044.01 µg g-1) while the highest accumulation of kaempferol-3-glucoside (1537.38 µg g-1), caffeic acid (130.13 µg g-1) and 4-hydroxy benzoic acid (465.93 µg g-1) was recorded in the 70% EtOH, aqueous, and EtOAc extracts, respectively. The EtOH (46.86 mg TE/g) and 70% EtOH (46.33 mg TE/g) extracts displayed the highest DPPH radical scavenging result. Both these extracts, along with the aqueous one, exerted the highest ABTS radical scavenging result (73.03-73.56 mg TE/g). The EtOH and 70% EtOH extracts revealed the most potent anti-AChE (2.66 and 2.64 mg GALAE/g) and anti-glucosidase (1.07 and 1.09 mmol ACAE/g) activities. The aqueous extract was the most efficacious in inhibiting the proliferation of prostate cancer (DU-145) cells with an IC50 of 8.71 µg/mL and a Selectivity Index of 3.7. In conclusion, this study appraised the use of V. diversifolium aerial parts as a potential therapeutic source for future development of phytopharmaceuticals that target specific oxidative stress-linked diseases including diabetes, cancer, cardiovascular disease, and Alzheimer's disease among others.
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Brain edema formation is a key factor for secondary tissue damage after traumatic brain injury (TBI), however, the type of brain edema and the temporal profile of edema formation are still unclear. We performed free water imaging, a bi-tensor model based diffusion MRI analysis, to characterize vasogenic brain edema (VBE) and cytotoxic edema (CBE) formation up to 7 days after experimental TBI. Male C57/Bl6 mice were subjected to controlled cortical impact (CCI) or sham surgery and investigated by MRI 4h, 1, 2, 3, 5, and 7 days thereafter (n = 8/group). We determined mean diffusivity (MD) and free water (FW) in contusion, pericontusional area, ipsi- and contralateral brain tissue. Free (i.e., non-restricted) water was interpreted as VBE, restricted water as CBE. To verify the results, VBE formation was investigated by in-vivo 2-Photon Microscopy (2-PM) 48h after surgery. We found that MD and FW values decreased for 48h within the contusion, indicating the occurrence of CBE. In pericontusional tissue, MD and FW indices were increased at all time points, suggesting the formation of VBE. This was consistent with our results obtained by 2-PM. Taken together, CBE formation occurs for 48h after trauma and is restricted to the contusion, while VBE forms in pericontusional tissue up to 7 days after TBI. Our results indicate that free water magnetic resonance imaging may represent a promising tool to investigate vasogenic and cytotoxic brain edema in the laboratory and in patients.
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Edema Encefálico , Lesões Encefálicas Traumáticas , Contusões , Humanos , Masculino , Camundongos , Animais , Edema Encefálico/diagnóstico por imagem , Edema Encefálico/etiologia , Edema Encefálico/patologia , Lesões Encefálicas Traumáticas/complicações , Lesões Encefálicas Traumáticas/diagnóstico por imagem , Lesões Encefálicas Traumáticas/patologia , Imagem de Difusão por Ressonância Magnética/métodos , Imageamento por Ressonância Magnética/métodos , Edema , ÁguaRESUMO
Incomplete reperfusion of the microvasculature ('no-reflow') after ischaemic stroke damages salvageable brain tissue. Previous ex vivo studies suggest pericytes are vulnerable to ischaemia and may exacerbate no-reflow, but the viability of pericytes and their association with no-reflow remains under-explored in vivo. Using longitudinal in vivo two-photon single-cell imaging over 7â days, we showed that 87% of pericytes constrict during cerebral ischaemia and remain constricted post reperfusion, and 50% of the pericyte population are acutely damaged. Moreover, we revealed ischaemic pericytes to be fundamentally implicated in capillary no-reflow by limiting and arresting blood flow within the first 24â h post stroke. Despite sustaining acute membrane damage, we observed that over half of all cortical pericytes survived ischaemia and responded to vasoactive stimuli, upregulated unique transcriptomic profiles and replicated. Finally, we demonstrated the delayed recovery of capillary diameter by ischaemic pericytes after reperfusion predicted vessel reconstriction in the subacute phase of stroke. Cumulatively, these findings demonstrate that surviving cortical pericytes remain both viable and promising therapeutic targets to counteract no-reflow after ischaemic stroke.
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Isquemia Encefálica , AVC Isquêmico , Acidente Vascular Cerebral , Humanos , Pericitos/fisiologia , Infarto CerebralRESUMO
This research investigates the potential use of Jurinea mesopotamica Hand.-Mazz. (Asteraceae) in cancer treatment. In this study, a plant extract was prepared using all parts of J. mesopotamica, and its effect on the proliferation of cancer and normal cells was tested using the MTT method. It was found to have a selective cytotoxic effect on prostate cancer cells, with the lowest IC50 (half-maximal inhibitory concentration) of 10µg/mL found in the butanol extract (JMBE). The extract suppressed the proliferation of prostate cancer cells (67 %), disrupted organelle integrity (49 %), increased reactive oxidative stress (66 %), and triggered cell death (51 %). In addition, apoptotic gene expressions and protein levels increased, and the profile of amino acids related to energy metabolism was elevated. Based on LC-MS/MS results, the plant contained higher levels of flavonoids, including isoquercitrin, cosmosiin, astragalin, nicotiflorin, luteolin, and apigenin. These results suggest that J. mesopotamica has a selective effect on prostate cancer due to its high flavonoid content and might be a promising natural alternative for cancer treatment.
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Asteraceae , Neoplasias da Próstata , Masculino , Humanos , Cromatografia Líquida , Apoptose , Espectrometria de Massas em Tandem , Neoplasias da Próstata/tratamento farmacológico , Flavonoides/química , Extratos Vegetais/farmacologia , Extratos Vegetais/química , Linhagem Celular TumoralRESUMO
In this context, phytochemicals were extracted from Ranunculus constantinopolitanus using ethyl acetate (EA), ethanol, ethanol/water (70%), and water solvent. The analysis encompassed quantification of total phenolic and flavonoid content using spectrophotometric assays, chemical profiling via high performance liquid chromatography-mass spectrometry/mass spectrometry (HPLC-MS/MS) for the extracts, and assessment of antioxidant activity via 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), Cupric reducing antioxidant capacity (CUPRAC), ferric reducing antioxidant power (FRAP), metal chelating (MCA), and phosphomolybdenum (PBD) assays. Moreover, antimicrobial activity was assessed against four different bacterial strains, as well as various yeasts. Enzyme inhibitory activities were evaluated against five types of enzymes. Additionally, the extracts were examined for their anticancer and protective effects on several cancer cell lines and the human normal cell line. All of the extracts exhibited significant levels of ferulic acid, kaempferol, and caffeic acid. All tested extracts demonstrated antimicrobial activity, with Escherichia coli and Pseudomonas aeruginosa being most sensitive to EA and ethanol extracts. Molecular docking studies revealed that kaempferol-3-O-glucoside strong interactions with AChE, BChE and tyrosinase. In addition, network pharmacology showed an association between gastric cancer and kaempferol-3-O-glucoside. Based on the results, R. constantinopolitanus can be a potential reservoir of bioactive compounds for future bioproduct innovation and pharmaceutical industries.
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Anti-Infecciosos , Ranunculus , Humanos , Antioxidantes/química , Extratos Vegetais/farmacologia , Extratos Vegetais/química , Espectrometria de Massas em Tandem , Simulação de Acoplamento Molecular , Água , Etanol , Anti-Infecciosos/farmacologia , Anti-Infecciosos/análiseRESUMO
OBJECTIVES: Substance use and aggressive behaviour pattern is considered as a serious criminal behaviour threat. This analytical cross-sectional study was designed to determine the level of aggression of convicts with substance use and to determine the variables predicting the substance use characteristics of convicts. METHODS: The study was carried out with 255 male convicts in 2 different closed penal execution institutions in Izmir province. In order to measure demographic variables, data were collected using the Information Form, Buss Perry Aggression Questionnaire and Addiction Profile Index. RESULTS: When convicts were examined according to demographic variables, it was found that there was a significant difference between the groups in terms of variables such as age, marital status, domestic violence, history of psychological treatment and self-harm behaviour. The results showed that 85.5% of convicts had used cannabis more than three times in their lifetime. Also there was a significant difference between the total score obtained from the aggression scale and the number of substance types used. CONCLUSIONS: Multidisciplinary approach in the field of addiction can go a long way in solving the problem and will play an important role in the protection of society, individuals and victims.
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The brain has a high energy demand but little to no energy stores. Therefore, proper brain function relies on the delivery of glucose and oxygen by the cerebral vasculature. The regulation of cerebral blood flow (CBF) occurs at the level of the cerebral capillaries and is driven by a fast and efficient crosstalk between neurons and vessels, a process termed neurovascular coupling (NVC). Experimentally NVC is mainly triggered by sensory stimulation and assessed by measuring either CBF by laser Doppler fluxmetry, laser speckle contrast imaging (LSCI), intrinsic optical imaging, BOLD fMRI, near infrared spectroscopy (NIRS) or functional ultrasound imaging (fUS). Since these techniques have relatively low spatial resolution, diameters of cerebral vessels are mainly assessed by 2-photon microscopy (2-PM). Results of studies on NVC rely on stable animal physiology, high-quality data acquisition, and unbiased data analysis, criteria, which are not easy to achieve. In the current study, we assessed NVC using two different imaging modalities, i.e., LSCI and 2-PM, and analyzed our data using an investigator-independent Matlab-based analysis tool, after manually defining the area of analysis in LSCI and vessels to measure in 2-PM. By investigating NVC in 6-8 weeks, 1-, and 2-year-old mice, we found that NVC was maximal in 1-year old mice and was significantly reduced in aged mice. These findings suggest that NVC is differently affected during the aging process. Most interestingly, specifically pial arterioles, seem to be distinctly affected by the aging. The main finding of our study is that the automated analysis tool works very efficiently in terms of time and accuracy. In fact, the tool reduces the analysis time of one animal from approximately 23 h to about 2 s while basically making no mistakes. In summary, we developed an experimental workflow, which allows us to reliably measure NVC with high spatial and temporal resolution in young and aged mice and to analyze these data in an investigator-independent manner.
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BACKGROUND: Teas and traditional herbs are taken as health remedies in most cultures worldwide. Plants often used in the manufacture of teas and herbal remedies can comprise carcinogenic contaminates, such as nitrates, nitrites and bromate ions. In the present study, a quick method was applied for the simultaneous determination of nitrate (NO3 - ), nitrite (NO2 - ) and bromate (BrO3 - ) ions in some selected plants and tea samples using capillary electrophoresis and a Box-Behnken response surface design for method optimization. RESULTS: Optimal conditions of 60 mmol L-1 phosphate buffer solution at pH 3.5, a capillary temperature of 40 °C, applied voltage of -22.5 kV, total capillary length of 61.5 cm (effective length 53.5 cm) with capillary internal diameter of 50 µm, and an injection pressure of 50 mbar for 30 s were selected. The limits of detection for nitrate, bromate and nitrite were 0.49, 1.25 and 0.11 mg L-1 , whereas linearity values were determined as 0.998, 0.996 and 0.999, respectively. CONCLUSION: A quick and easy applicable method was developed and the detection of the anions was achievable within 6 min of analysis. From the studied samples (cherry stalk, a dry mixed herbal tea; cherry stalk, dry sticks; dry pomegranate flower; dry olive leaves; and black tea), dry pomegranate flower (13.10 ± 0.62 mg L-1 ) and cherry stalk, dry mix herbal tea (13.10 ± 0.62 mg L-1 ) had the highest nitrate content. © 2021 Society of Chemical Industry.
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Eletroforese Capilar/métodos , Nitratos/análise , Nitritos/análise , Plantas/química , Bromatos , Limite de DetecçãoRESUMO
BACKGROUND: Leukocytes contribute to tissue damage after cerebral ischemia; however, the mechanisms underlying this process are still unclear. This study investigates the temporal and spatial relationship between vascular leukocyte recruitment and tissue damage and aims to uncover which step of the leukocyte recruitment cascade is involved in ischemic brain injury. METHODS: Male wild-type, ICAM-1-deficient, anti-CD18 antibody treated, or selectin-deficient [fucusyltransferase (FucT IV/VII-/-)] mice were subjected to 60 min of middle cerebral artery occlusion (MCAo). The interaction between leukocytes and the cerebrovascular endothelium was quantified by in vivo fluorescence microscopy up to 15 h thereafter. Temporal dynamics of neuronal cell death and leukocyte migration were assessed at the same time points and in the same tissue volume by histology. RESULTS: In wild-type mice, leukocytes started to firmly adhere to the wall of pial postcapillary venules two hours after reperfusion. Three hours later, neuronal loss started and 13 h later, leukocytes transmigrated into brain tissue. Loss of selectin function did not influence this process. Application of an anti-CD18 antibody or genetic deletion of ICAM-1, however, significantly reduced tight adhesion of leukocytes to the cerebrovascular endothelium (-60%; p < 0.01) and increased the number of viable neurons in the ischemic penumbra by 5-fold (p < 0.01); the number of intraparenchymal leukocytes was not affected. CONCLUSIONS: Our findings suggest that ischemia triggers only a transient adhesion of leukocytes to the venous endothelium and that inhibition of this process is sufficient to partly prevent ischemic tissue damage.
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BACKGROUND: Polycyclic aromatic hydrocarbons (PAH) are considered to be one of the major contaminants of drinking water and natural water bodies. Some of the well documented polycyclic aromatic hydrocarbons that are water pollutants and were considered for analysis in this study included benzo[b]fluoranthene (BbF), benzo[k]fluoranthene (BkF), benzo[a]pyrene (BaP), benzo[g,h,i]perylene (BgP), and indeno[1,2,3-c,d]pyrene (InD). This study aimed at determining the levels of concentrations of basically five polycyclic aromatic hydrocarbons in 57 drinking water bodies located around Samsun, Ordu, Giresun, Çorum, Amasya, Kastamonu and Sinop provinces. MATERIALS AND METHOD: In this study, the Environmental Protection Agency (EPA) method 550.1 for the determination of polycyclic aromatic hydrocarbons in drinking by Liquid-Solid Extraction (LSE) and High Performance Liquid Chromatography (HPLC) with Coupled Ultraviolet (CD) and Fluorescence Detection (FD) was used. Sampling procedures were done according to the validated method specified by the Turkish Ministry of Enivironment and Forestry. Prior to the determination of concentrations by HPLC, PAHs contained in the samples were separated from the solid phase by Solid-Phase Extraction (SPE). All data analyses were conducted using SPSS and Excel. RESULTS: Obtained results from the investigation revealed that the average total PAH and benzo[a]pyrene (BaP) concentration levels in drinking water samples taken from the central districts of Samsun were 2.73 ± 1.51 and 0.35 ± 0.24 ng/L respectively. In drinking water samples taken from Ordu, Giresun, Çorum, Amasya, Kastamonu and Sinop, the average total PAH concentrations were found to be 5.85 ± 3.82 ng/L, 3.79 ± 1.27 ng/L, 1.08 ± 0.62 ng/L, 2.42 ± 1.04 ng/L; 1.92 ± 0.35 ng/L and 4.07 ± 2.33 ng/L respectively. The average (BaP) concentrations for the same named locations were determined as 0.97 ± 0.75 ng/L; 0.55 ± 0.29 ng/L; 0.11 ± 0.08 ng/L; 0.35 ± 0.10 ng/L; 0.14 ± 0.04 ng/L; 0.39 ± 0.23 ng/L, respectively. It is therefore evident that the values ââof PAH and BaP in drinking water were below the limits of 100 and 10 ng/L ââspecified in the Regulation on Water Intended for Human Consumption. These values are below the set limits proposed by Turkish legislation and WHO. CONCLUSION: All the results for drinking water, usable water and natural spring water were below the values ââspecified in the Regulation on Water Intended for Human Consumption and WHO. The PAH content of the studied river waters as well were below the limits proposed by Turkish legislation and WHO.
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OBJECTIVES: Folic acid (FA) is physiologically important in mammals and is a common vitamin supplement used during pregnancy and lactation. Numerous studies have reported that FA significantly improves endothelial function. The blood-brain barrier (BBB) plays an important role in maintaining the microenvironment required for neuronal function, but its unique structure is damaged by epileptic seizures. The aim of this study was to evaluate the potential protective role of FA on BBB leakage, as well as on the reactive astrogliosis in pregnant rats and their prepubertal offspring during pentylenetetrazole (PTZ)-induced epileptic seizure. METHODS: Pregnant rats were treated with FA (5 mg/kg) and PTZ on gestational days 0-19 and 19, respectively. The pups were treated with PTZ at puberty. Evans blue was used to evaluate BBB integrity. Reactive astrogliosis was defined using immunohistochemical analysis for glial fibrillary acidic protein (GFAP). Mean arterial blood pressure (MABP) was measured at the femoral artery. RESULTS: A moderate decrease in BBB leakage was observed in FA-treated pregnant and prepubertal animals (P < 0.05). MABP was decreased significantly in pregnant rats (P < 0.05). The epilepsy-induced increase in MABP was less prominent in pregnant animals (P < 0.05). GFAP intensity decreased in PTZ-treated pregnant animals (P < 0.01) and FA-treated prepubertal rats. DISCUSSION: Our findings suggest that FA, which is used as a maternal vitamin to promote normal fetus development, may be beneficial against seizure-induced neuronal damage by decreasing BBB leakage and reactive astrogliosis in pregnant and prepubertal rats.
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Barreira Hematoencefálica/efeitos dos fármacos , Ácido Fólico/farmacologia , Pentilenotetrazol/efeitos adversos , Convulsões/tratamento farmacológico , Animais , Pressão Sanguínea/efeitos dos fármacos , Barreira Hematoencefálica/metabolismo , Epilepsia/induzido quimicamente , Epilepsia/tratamento farmacológico , Feminino , Ácido Fólico/administração & dosagem , Proteína Glial Fibrilar Ácida/metabolismo , Fenômenos Fisiológicos da Nutrição Materna , Gravidez , Ratos , Ratos Wistar , Convulsões/induzido quimicamenteRESUMO
Apelin is a novel bioactive peptide as the endogenous ligand for APJ. Apelin and APJ have also been identified in the testis, hypothalamic nuclei such as arcuate, supraoptic and paraventricular nuclei, implicating roles in the control of reproduction. Therefore, the present study was designed to investigate the effects of chronic central infusion of apelin-13 on LH, FSH and testosterone levels and testis morphology. 21 Wistar-Albino male rats received continuous intracerebroventricular infusion via Alzet osmotic mini pumps filled artificial cerebrospinal fluid (vehicle) or apelin-13 at concentrations of 1 or 10 nmol (10 µl/h) for seven days. At the last 90 min of the infusion period, the blood samples were collected at 15 min intervals (0-90 min) for LH and FSH analyses. At the last sampling point, the blood samples were analyzed for testosterone levels. Infusion of high dose apelin-13 significantly suppressed LH release compared with the vehicle values at 30, 60 and 75 min (p<0.05). However, FSH levels did not significantly differ among the groups. Serum testosterone levels in high dose apelin-13 group were statistically lower than the control group (p<0.05). In addition, histological examination showed that infusion of high dose apelin-13 significantly decreased the number of Leydig cells compared with the control and lower dose apelin-13 groups (p<0.05, p<0.01). Our results suggest that apelin-13 may play a role in the central regulation and decreases testosterone release by suppressing LH secretion. Thus, antagonists of the apelin receptor may, therefore, be useful for pharmaceuticals in the treatment of infertility.
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Peptídeos e Proteínas de Sinalização Intercelular/farmacologia , Testículo/efeitos dos fármacos , Animais , Receptores de Apelina , Hormônio Foliculoestimulante/sangue , Infusões Intraventriculares , Peptídeos e Proteínas de Sinalização Intercelular/metabolismo , Células Intersticiais do Testículo/citologia , Células Intersticiais do Testículo/efeitos dos fármacos , Ligantes , Hormônio Luteinizante/sangue , Masculino , Ratos Wistar , Receptores Acoplados a Proteínas G/metabolismo , Túbulos Seminíferos/citologia , Túbulos Seminíferos/efeitos dos fármacos , Testículo/citologia , Testosterona/sangueRESUMO
We evaluated the effect of zinc treatment on the blood-brain barrier (BBB) permeability and the levels of zinc (Zn), natrium (Na), magnesium (Mg), and copper (Cu) in the brain tissue during epileptic seizures. The Wistar albino rats were divided into four groups, each as follows: (1) control group, (2) pentylenetetrazole (PTZ) group: rats treated with PTZ to induce seizures, (3) Zn group: rats treated with ZnCl(2) added to drinking water for 2 months, and (4) Zn + PTZ group. The brains were divided into left, right hemispheres, and cerebellum + brain stem regions. Evans blue was used as BBB tracer. Element concentrations were analyzed by inductively coupled plasma optical emission spectroscopy. The BBB permeability has been found to be increased in all experimental groups (p < 0.05). Zn concentrations in all brain regions in Zn-supplemented groups (p < 0.05) showed an increase. BBB permeability and Zn level in cerebellum + brain stem region were significantly high compared to cerebral hemispheres (p < 0.05). In all experimental groups, Cu concentration decreased, whereas Na concentrations showed an increase (p < 0.05). Mg content in all the brain regions decreased in the Zn group and Zn + PTZ groups compared to other groups (p < 0.001). We also found that all elements' levels showed hemispheric differences in all groups. During convulsions, Zn treatment did not show any protective effect on BBB permeability. Chronic Zn treatment decreased Mg and Cu concentration and increased Na levels in the brain tissue. Our results indicated that Zn treatment showed proconvulsant activity and increased BBB permeability, possibly changing prooxidant/antioxidant balance and neuronal excitability during seizures.
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Barreira Hematoencefálica/efeitos dos fármacos , Cloretos/farmacologia , Convulsões/metabolismo , Compostos de Zinco/farmacologia , Animais , Pressão Sanguínea , Barreira Hematoencefálica/metabolismo , Barreira Hematoencefálica/patologia , Tronco Encefálico/efeitos dos fármacos , Tronco Encefálico/metabolismo , Cerebelo/efeitos dos fármacos , Cerebelo/metabolismo , Cloretos/administração & dosagem , Convulsivantes/farmacologia , Cobre/metabolismo , Azul Evans/metabolismo , Magnésio/metabolismo , Pentilenotetrazol/efeitos adversos , Permeabilidade , Ratos , Ratos Wistar , Convulsões/induzido quimicamente , Convulsões/tratamento farmacológico , Convulsões/patologia , Sódio/metabolismo , Zinco/metabolismo , Compostos de Zinco/administração & dosagemRESUMO
In this investigation, the effects of hypoglycemic coma and alcoholic coma on the blood-brain barrier (BBB) permeability have been compared. Female adult Wistar albino rats weighing 180-230 g were divided into three groups: Control group (n=8), Alcoholic Coma Group (n=18), and Hypoglycemic Coma group (n=12). The animals went into coma approximately 3-4 hours after insulin administration and 3-5 minutes after alcohol administration. Evans blue (4mL/kg) was injected intravenously as BBB tracer. It was observed that the alcoholic coma did not significantly increase the BBB permeability in any of the brain regions when compared to control group. Changes in BBB permeability were significantly increased by the hypoglycemic coma in comparison to the control group values (p<0.01). Our findings suggest that hypoglycemic and alcoholic coma have different effects on the BBB permeability depending on the energy metabolism.
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Intoxicação Alcoólica/fisiopatologia , Barreira Hematoencefálica/fisiopatologia , Coma/fisiopatologia , Hipoglicemia/fisiopatologia , Coma Insulínico/fisiopatologia , Animais , Glicemia/metabolismo , Pressão Sanguínea , Barreira Hematoencefálica/efeitos dos fármacos , Modelos Animais de Doenças , Metabolismo Energético/efeitos dos fármacos , Etanol/toxicidade , Feminino , Insulina/administração & dosagem , Permeabilidade/efeitos dos fármacos , Ratos , Ratos WistarRESUMO
A simultaneous determination method for the analysis of benzo[a]pyrene (BaP) and dibenzo[a,h]anthracene (DahA) in the filter tar of Turkish cigarettes has been developed. The method involved (a) the extraction of BaP and DahA with n-hexane from ACN solution in which the cigarette filters were extracted, and then (b) purification of the n-hexane extracts by elution on an XAD-2 column using n-hexane/dichloromethane (9:1, v/v) mixture. Separation and quantitative determination of BaP and DahA in the extracts were carried out by HPLC and fluorescence detection on a C18 RP column. The calculated recoveries for BaP and DahA were found in the range of 90-100% for each extraction and clean-up steps. Analysis of various filter tar of Turkish cigarettes showed that an average of 74.28 ng/filter of BaP and 5.24 ng/filter of DahA were present in Turkish cigarettes.