Detection of subwavelength slit-width variation with irradiance measurements in the far field.

We demonstrate that, under suitable conditions, subwavelength feature variations of an object can affect the corresponding far-field diffraction pattern in a measurable way. We present an experiment in which width variations of less than 1/100 of the wavelength are measured with a slit whose width is 100 times the wavelength. Integral and differential intensity measurements in the far field are fully consistent with standard diffraction theory even in the subwavelength variation regime. In particular, slit modulations of 6 nm with a wavelength of 670 nm are shown to follow theoretical calculations within the experimental sensitivity of ~10(-5) .

Molecular structure of DNA by scanning tunneling microscopy.

Uncoated DNA molecules marked with an activated tris(l-aziridinyl) phosphine oxide (TAPO) solution were deposited on gold substrates and imaged in air with the use of a high-resolution scanning tunneling microscope (STM). Constant-current and gap-modulated STM images show clear evidence of the helicity of the DNA structure: pitch periodicity ranges from 25 to 35 angstroms, whereas the average diameter is 20 angstroms. Molecular structure within a single helix turn was also observed.