Synthesis of Copper Molybdate and Its Electrochemical Sensing of Paracetamol.

Background In recent years, significant advancements have been made in various scientific sectors, particularly in healthcare and pharmaceutical research. This progress has been driven by the development of enhanced sensing materials and methodologies. Electrochemical sensing has become an important tool in detecting and analyzing drug molecules due to its high sensitivity, specificity, and rapid response times. Among various drugs, paracetamol, also known as acetaminophen, is widely used for its analgesic and antipyretic properties. Accurate detection of paracetamol is crucial due to its widespread use and potential for overdose, which can lead to severe liver damage. Copper molybdate (CuMoO4) is a transition metal oxide that has garnered attention for its excellent electrical conductivity and electrochemical stability. These properties make it a promising candidate for use in electrochemical sensors. The ability of CuMoO4 to act as a sensor material is enhanced by its unique structural and morphological characteristics, which can be tailored during synthesis. Aim This study aimed to synthesize CuMoO4 and investigate its electrochemical sensing capability for the detection of drug molecules, specifically paracetamol. Materials and method CuMoO4 was synthesized using a precipitation method that did not involve any surfactants. This approach was chosen to simplify the synthesis process and avoid potential contamination from surfactants. The morphology of the synthesized CuMoO4 nanoparticles was investigated using a field emission scanning electron microscope (FE-SEM). Energy-dispersive X-ray spectroscopy (EDX) confirmed the purity of the CuMoO4 nanomaterial. Structural analysis was performed using X-ray diffraction (XRD). To evaluate the electrochemical sensing capability of CuMoO4 for paracetamol, Differential pulse voltammetry (DPV) was employed. DPV is a sensitive electrochemical technique that can detect changes in current response corresponding to the presence of analytes. Results The synthesized CuMoO4 exhibited a rock-like structure, as revealed by FE-SEM imaging. This morphology is advantageous for electrochemical applications due to the increased surface area available for interaction with analytes. EDX confirmed the purity of the CuMoO4 nanomaterial, showing no significant impurities. XRD analysis indicated that the CuMoO4 nanoparticles were crystalline in nature, which is beneficial for consistent and reproducible electrochemical behavior. The DPV analysis demonstrated that the CuMoO4 sensor exhibited a linear increase in current response with increasing concentrations of paracetamol. This linear relationship indicates that CuMoO4 is capable of detecting paracetamol effectively, with a strong and quantifiable signal response. Conclusion The CuMoO4 nanomaterial was successfully synthesized using a simple precipitation method and was characterized by its rock-like morphology and crystalline structure. Electrochemical testing using DPV showed that CuMoO4 has excellent sensing capabilities for detecting paracetamol, with a clear and linear current response. These findings suggest that CuMoO4 is a promising electrochemical sensing material for drug detection, potentially offering a reliable and efficient method for monitoring paracetamol and possibly other pharmaceuticals in various settings.

Electrochemical Detection of Ascorbic Acid by Fe2O3 Nanoparticles Modified Glassy Carbon Electrode.

Background  The article delineates a strategy for detecting ascorbic acid (AA) through the use of iron oxide (Fe2O3) nanoparticles on an electrode. The Fe2O3 nanoparticles demonstrated effective electrocatalysis in the oxidation of AA, resulting in increased peak currents. The sensor showcased a wide linear detection range, a low detection limit, and high selectivity towards interferents, making it suitable for accurate AA measurement in food analysis and medical diagnostics applications. This emphasizes the potential of Fe2O3 nanoparticle-based sensors for precise AA detection. Aim The primary aim of this research is to develop an electrochemical sensing technique for the identification of ascorbic acid, with the use of Fe2O3 nanoparticles as the sensing matrix. Materials and methods The synthesis process involved the utilization of FeCl3.6H2O, ammonia solution, ethanol, and double-distilled water. FeCl3.6H2O was dissolved in ammonia water to produce a brown precipitate for the synthesis of Fe2O3 nanoparticles. Subsequently, the brown precipitate underwent hydrothermal treatment at 180 °C, resulting in the formation of a red product. Following centrifugation, washing, and drying steps, Fe2O3 nanoparticles were successfully synthesized. These nanoparticles were then utilized to modify the glassy carbon electrode (GCE). Prior to the modification, the GCE underwent polishing and cleaning procedures, after which it was coated with a suspension containing 5 mg of Fe2O3 nanoparticles in 10 mL of ethanol. The coated electrode was dried and deemed ready for application in electrochemical sensing. Results The hydrothermal method was employed in this research to synthesize Fe2O3 nanoparticles, which were subsequently subjected to a series of experiments to evaluate their electrochemical sensing capabilities. The resulting Fe2O3 nanoparticles were determined to possess a high level of purity and a crystalline structure through various analyses, including field emission-scanning electron microscopy (FE-SEM), cyclic voltammetric testing, X-ray diffraction (XRD), energy-dispersive X-ray (EDX) spectroscopy analysis, differential pulse voltammetry (DPV), and the current response of the Fe2O3-modified electrode towards ascorbic acid. The morphology of the Fe2O3 nanoparticles was observed to be uniform. The synthesized particles successfully fulfilled the study's objective by exhibiting remarkably sensitive and selective sensitivity towards ascorbic acid. Conclusion Our study underscores the potential of utilizing Fe2O3 nanoparticle-based electrochemical sensing to detect ascorbic acid, as evidenced by the notably high sensitivity of ascorbic acid towards Fe2O3 nanoparticles. The distinctive properties of Fe2O3 nanoparticles, which include their large surface area, efficient electron transport, and straightforward manufacturing process, significantly enhance the sensor's performance. Further research is crucial to exploring the wide-ranging applications of the sensor in fields such as food safety, environmental monitoring, and biological diagnostics and to overcome any existing limitations.

The Synthesis of FeS and Investigation on Electrochemical Sensing Toward Neuroprotector.

Background Electrochemical sensing is a versatile field that uses electrochemistry concepts to detect and measure various substances. It finds applications in clinical diagnostics and environmental monitoring. Scientists are currently working on creating reliable electrochemical sensing devices that can accurately detect ascorbic acid. Iron sulfide (FeS) has emerged as a promising material for these sensors due to its excellent electrical conductivity, catalytic activity, and stability.  Materials and methods The FeS nanoparticles were synthesized through the hydrothermal method of synthesis. The glassy carbon electrode (GCE) with a surface area of 0.071 cm2 was modified with FeS before the working electrode was mechanically polished with 1 µm, 0.3 µm, and 0.05 µm alumina pastes for mirror finishing. Then it was subjected to ultrasonication in double distilled water for a few minutes to clean the surface of GCE. The FeS suspension was prepared by dispersing 5 mg of FeS in 10 mL of ethanol during 20 minutes of ultrasonic agitation then the GCE was coated with 10 µL of the suspension by drop coating method and dried in air. Results In this study, FeS nanoparticles were synthesized by the hydrothermal method of synthesis, and it was tested for their electrochemical sensing properties by various tests. Based on the field emission-scanning electron microscope (FE-SEM) analysis, scan rate effect test, cyclic voltammetric test, X-ray diffraction (XRD), and energy-dispersive X-ray (EDX) spectroscopy analysis done and results obtained, it was seen that the synthesized FeS nanoparticles are highly pure and have a crystalline structure. FeS has an even morphology. The synthesized particles also showed highly sensitive and specific sensing toward ascorbic acid when compared to unmodified 10.1 µA electrodes with a sensing value of 12.51 µA, thereby fulfilling the aim of this study. Conclusion Based on the outcomes of the diverse tests carried out, it is evident that the sample displayed a high crystalline nature as indicated by the XRD test. Additionally, the sample exhibited a uniform morphology, exceptional stability, and remarkable sensitivity. The developed FeS-based electrochemical sensor was found to be exceptionally pure and showed excellent performance, showcasing both high sensitivity and selectivity toward ascorbic acid.

Anti-metastatic Effect of Heliotropium bacciferum on MCF-7 Human Breast Cancer Cell Line.

Background Heliotropium bacciferum, often known as wild heliotrope or wild quailplant, is a flowering plant from the borage family. This study examines the anti-metastatic impact of H. bacciferum on Michigan Cancer Foundation-7 (MCF-7) breast cancer cells and its ability to disrupt signaling pathways. Aim To explore the anti-metastatic effect of H. bacciferum on the MCF-7 breast cancer cell line. Materials and methods For this research, MCF-7 breast cancer cells were used. Cells were cultured and subjected to 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, as well as gene expression analysis for glycogen synthase kinase 3 beta (GSK3ß), wingless-related integration site 2 (Wnt2), and ß-catenin. The plant extract was tested to determine if it successfully blocked the signalling pathway or not.  Results The MTT test was performed to study the cytotoxic impact of H. bacciferum. At an increasing concentration of 100 µg/mL, the extract inhibited growth by 55%, whereas at 150 µg/mL, it inhibited growth by 52.5%. Maximum inhibition was seen at 150 µg/mL. H. bacciferum suppressed the GSK3ß and Wnt2 signaling pathways in MCF-7 breast cancer cell lines, acting as an anti-metastatic and anticancer agent. The heliotrine compound in H. bacciferum showed high binding energy to metastatic targets such as GSK3ß, Wnt2, and ß-catenin. Moreover, chemical absorption, distribution, metabolism, excretion, and toxicity (ADMET) properties also support the study. Conclusion In this study, we can infer that H. bacciferum has a favourable anticancer impact on MCF-7 breast cancer cell lines and may be utilised as an anticancer drug against breast cancer cells. It can also be further evaluated for different breast cancers and cell lines.

Sensing of Quercetin With Cobalt-Doped Manganese Nanosystems by Electrochemical Method.

BACKGROUND: The pressing need for precise, quick, and affordable detection of diverse biomolecules has led to notable developments in the realm of biosensors. Quercetin, a biomolecule rich in flavonoids predominantly found in our diet, is sensed by the electrochemical method. The electrochemical properties show remarkable improvement when Mn2O3 (MO) is doped with cobalt (Co).  Aim: This study aimed to investigate the biomolecule sensing of quercetin using Co-doped MO by electrochemical method.  Materials and methods: Co-doped MO nanospheres were prepared by hydrothermal method. The crystal structure of the synthesized material was evaluated by using X-ray diffraction analysis. The sample morphology was assessed by using field emission scanning electron microscopy (FE-SEM) techniques. The cyclic voltammetry technique was used for the detection of quercetin biomolecules.  Results: The synthesized Co-doped MO appeared to be spherical in morphology in FE-SEM. Energy-dispersive X-ray spectroscopy showed the only presence of Co, Mn, and O, which confirmed the purity of the sample. The modified electrode sensed the biomolecule with a higher current of 7.35 µA than the bare glassy carbon electrode of 6.1 µA. CONCLUSION: The Co-doped MO exhibited enhanced conductivity, reactivity, and electrochemical performance. This tailored approach will help in the optimization of material properties toward specific biomolecule sensing applications.

Microwave-Assisted Synthesis of Flower-like MnMoO4 Nanostructures and Their Photocatalytic Performance.

This article describes an affordable method for the synthesis of MnMoO4 nanoflowers through the microwave synthesis approach. By manipulating the reaction parameters like solvent, pH, microwave power, and irradiation duration along this pathway, various nanostructures can be acquired. The synthesized nanoflowers were analyzed by using a powder X-ray diffractometer (XRD), field emission scanning electron microscopy (FE-SEM) with energy dispersive X-ray spectroscopy (EDS), Fourier transform infrared spectroscopy (FT-IR), and UV-vis diffuse reflectance spectroscopy (UV-DRS) to determine their crystalline nature, morphological and functional group, and optical properties, respectively. X-ray photoelectron spectroscopy (XPS) was performed for the examination of elemental composition and chemical states by qualitative and quantitative analysis. The results of the investigations demonstrated that the MnMoO4 nanostructures with good crystallinity and distinct shape were formed successfully. The synthesized MnMoO4 nanoflowers were tested for their efficiency as a photocatalyst in the degradation studies of methylene blue (MB) as model organic contaminants in an aqueous medium under visible light, which showed their photocatalytic activity with a degradation of 85%. Through the band position calculations using the electronegative value of MnMoO4, the photocatalytic mechanism of the nanostructures was proposed. The results indicated that the effective charge separation, and transfer mechanisms, in addition to the flower-like shape, were responsible for the photocatalytic performance. The stability of the recovered photocatalyst was examined through its recyclability in the degradation of MB. Leveraging MnMoO4's photocatalytic properties, future studies may focus on scaling up these processes for practical and large-scale environmental remediation.

Merremia emarginata Extract Potentiates the Inhibition of Human Colon Cancer Cells (HT-29) via the Modulation of Caspase-3/Bcl-2-Mediated Pathways.

Background This study investigates Merremia emarginata's curative effectiveness against colon cancer cells. M. emarginata, often known as Elika jemudu, is a Convolvulaceae family plant. The inhibitory ability of anticancer herbal extracts against cancer cell growth and mediators is tested.  Aim This study aims to evaluate the potent anticancer activity of M. emarginata against colon cancer cell line (HT-29). Materials and methods M. emarginata leaves were gathered and processed using solvent extraction. Anticancer activity on colon cancer cells was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test and cysteine aspartic acid protease-3 (caspase 3), B-cell lymphoma 2 (Bcl-2), and B-cell lymphoma-extra large (Bcl-xL) mRNA expressions. The data was reported as the mean ± SD of three separate experiments done in triplicate. The statistical analysis was carried out using one-way analysis of variance (ANOVA), with a p-value less than 0.05 indicating statistical significance. Results The cell viability test showed a gradual decrease in cell growth and proliferation as the concentration increased. The ethanolic extract of M. emarginata was found to be cytotoxic against colon caller cell lines. The extract was able to induce apoptosis of cancer as revealed by Bcl-2, Bcl-xL, and caspase-3 (p<0.05 and p<0.001) signaling pathways. Conclusion M. emarginata extracts showed good anticancer activity against colon cancer cell lines. Further work is required to establish and identify the chemical constituent responsible for its anticancer activity.

Evaluation of Antibacterial and Anti-oxidant Activities of Suaeda monoica Extract for Its Potential Application.

BACKGROUND:  An assessment of Suaeda monoica extract's antimicrobial and antioxidant properties was undertaken in light of its possible application as an oral care product. The maintenance of optimal dental health is just as important as overall wellness. Food particles become trapped in the mouth cavity, making it easy for oral bacteria to infect. AIM:  The study sought to ascertain the antibacterial and antioxidant properties of salt marsh Suaeda monoica extract. MATERIALS AND METHODS:  Leaves of Suaeda monoica, collected, dried and powdered, were dissolved in 70% methanol and the extract of 25-100 µg/ml was analyzed for antioxidant activity through total antioxidant assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, and total reducing power. Suaeda monoica antibacterial activity was also performed and the minimum inhibitory concentration was determined for 75 µg/ml, 100µg/ml, and 150 µg/ml concentrations and tetracycline in 10mcg/disc as a control against three different oral pathogens: Staphylococcus mutans, Streptococcus aureus, and Klebsiella spp. RESULTS:  At varying concentrations of 75 mg/ml to 150 mg/ml, Suaeda monoica extracts are efficacious with varying concentrations against the investigated bacterial strains. In the present study, in the DPPH assay, total reducing power, and total antioxidant activity assay, there was an increase in inhibitory percentage as the concentration increased from 25-100 µg/ml, showing maximum inhibition at 100 µg/ml concentration. CONCLUSION:  The results of the investigation show that Suaeda monoica has significant antibacterial and antioxidant activity in a concentration-dependent manner and can be potentially used as an oral care agent after it is assessed for clinical use.

Ag@Ag8W4O16 nanoroasted rice beads with photocatalytic, antibacterial and anticancer activity.

Increasing resistance of pathogens and cancer cell line towards antibiotics and anticancer agents has caused serious health problems in the past decades. Due to these problems in recent years, researchers have tried to combine nanotechnology with material science to have intrinsic antimicrobial and anticancer activity. The metals and metal oxides were investigated with respect to their antimicrobial and anticancer effects towards bacteria and cancer cell line. In the present work metal@metal tungstate (Ag@Ag8W4O16 nanoroasted rice beads) is investigated for antibacterial activity against Escherichia coli and Staphylococcus aureus using Mueller-Hinton broth and the anticancer activity against B16F10 cell line was studied. Silver decorated silver tungstate (Ag@Ag8W4O16) was synthesized by the microwave irradiation method using Cetyl Trimethyl Ammonium Bromide (CTAB). Ag@Ag8W4O16 was characterized by using various spectroscopic techniques. The phase and crystalline nature were analyzed by using XRD. The morphological analysis was carried out using Field Emission Scanning Electron Microscopy (FE-SEM), and High Resolution Transmission Electron Microscopy (HR-TEM). Further, Fourier Transform Infrared Spectroscopy (FT-IR) and Raman spectral analysis were carried out in order to ascertain the presence of functional groups in Ag@Ag8W4O16. The optical property was investigated using Diffuse Reflectance Ultraviolet-Visible Spectroscopy (DRS-UV-Vis) and the band gap was found to be 3.08eV. Surface area of the synthesized Ag@Ag8W4O16 wasanalyzed by BET analysis and Ag@Ag8W4O16 was utilized for the degradation of organic dyes methylene blue and rhodamine B. The morphology of the Ag@Ag8W4O16 resembles roasted rice beads with breath and length in nm range. The oxidation state of tungsten (W) and silver (Ag) was investigated using X-ray photoelectron spectroscopy (XPS).