In-depth genome and pan-genome analysis of a metal-resistant bacterium Pseudomonas parafulva OS-1.

A metal-resistant bacterium Pseudomonas parafulva OS-1 was isolated from waste-contaminated soil in Ranchi City, India. The isolated strain OS-1 showed its growth at 25-45°C, pH 5.0-9.0, and in the presence of ZnSO4 (upto 5 mM). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain OS-1 belonged to the genus Pseudomonas and was most closely related to parafulva species. To unravel the genomic features, we sequenced the complete genome of P. parafulva OS-1 using Illumina HiSeq 4,000 sequencing platform. The results of average nucleotide identity (ANI) analysis indicated the closest similarity of OS-1 to P. parafulva PRS09-11288 and P. parafulva DTSP2. The metabolic potential of P. parafulva OS-1 based on Clusters of Othologous Genes (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) indicated a high number of genes related to stress protection, metal resistance, and multiple drug-efflux, etc., which is relatively rare in P. parafulva strains. Compared with other parafulva strains, P. parafulva OS-1 was found to have the unique ß-lactam resistance and type VI secretion system (T6SS) gene. Additionally, its genomes encode various CAZymes such as glycoside hydrolases and other genes associated with lignocellulose breakdown, suggesting that strain OS-1 have strong biomass degradation potential. The presence of genomic complexity in the OS-1 genome indicates that horizontal gene transfer (HGT) might happen during evolution. Therefore, genomic and comparative genome analysis of parafulva strains is valuable for further understanding the mechanism of resistance to metal stress and opens a perspective to exploit a newly isolated bacterium for biotechnological applications.

Deciphering the virulence factors, regulation, and immune response to Acinetobacter baumannii infection.

Deciphering the virulence factors, regulation, and immune response to Acinetobacter baumannii infectionAcinetobacter baumannii is a gram-negative multidrug-resistant nosocomial pathogen and a major cause of hospital acquired infetions. Carbapenem resistant A. baumannii has been categorised as a Priority1 critial pathogen by the World Health Organisation. A. baumannii is responsible for infections in hospital settings, clinical sectors, ventilator-associated pneumonia, and bloodstream infections with a mortality rates up to 35%. With the development of advanced genome sequencing, molecular mechanisms of manipulating bacterial genomes, and animal infection studies, it has become more convenient to identify the factors that play a major role in A. baumannii infection and its persistence. In the present review, we have explored the mechanism of infection, virulence factors, and various other factors associated with the pathogenesis of this organism. Additionally, the role of the innate and adaptive immune response, and the current progress in the development of innovative strategies to combat this multidrug-resistant pathogen is also discussed.

Biotechnological Applications of Probiotics: A Multifarious Weapon to Disease and Metabolic Abnormality.

Consumption of live microorganisms "Probiotics" for health benefits and well-being is increasing worldwide. Their use as a therapeutic approach to confer health benefits has fascinated humans for centuries; however, its conceptuality gradually evolved with methodological advancement, thereby improving our understanding of probiotics-host interaction. However, the emerging concern regarding safety aspects of live microbial is enhancing the interest in non-viable or microbial cell extracts, as they could reduce the risks of microbial translocation and infection. Due to technical limitations in the production and formulation of traditionally used probiotics, the scientific community has been focusing on discovering new microbes to be used as probiotics. In many scientific studies, probiotics have been shown as potential tools to treat metabolic disorders such as obesity, type-2 diabetes, non-alcoholic fatty liver disease, digestive disorders (e.g., acute and antibiotic-associated diarrhea), and allergic disorders (e.g., eczema) in infants. However, the mechanistic insight of strain-specific probiotic action is still unknown. In the present review, we analyzed the scientific state-of-the-art regarding the mechanisms of probiotic action, its physiological and immuno-modulation on the host, and new direction regarding the development of next-generation probiotics. We discuss the use of recently discovered genetic tools and their applications for engineering the probiotic bacteria for various applications including food, biomedical applications, and other health benefits. Finally, the review addresses the future development of biological techniques in combination with clinical and preclinical studies to explain the molecular mechanism of action, and discover an ideal multifunctional probiotic bacterium.

Perspective of ACC-deaminase producing bacteria in stress agriculture.

The 1-aminocyclopropane-1-carboxylate deaminase (ACCD) enzyme plays an important role in stress alleviation of both biotic and abiotic stressors in plants and thereby enhances their growth under harsh environmental conditions. In-depth analysis of AcdS gene encoding for ACC deaminase reveals its presence in diverse microorganisms including bacteria and fungi. Particularly, plant growth-promoting bacteria (PGPB) containing ACCD supports plant growth by modulating the level of 'stress ethylene' and cleaving its precursor 1-aminocyclopropane-1-carboxylic acid (ACC) into α-ketobutyrate and ammonia, enabling PGPB to utilize ACC as a carbon and nitrogen source. The reduced synthesis of ethylene in plants further relieves the ethylene inhibition of plant growth and development, and improves plant resistance to various stressors. Therefore, the dual role of microbial ACCD makes it a cost-effective and eco-friendly biocatalyst for sustainable agricultural productions. The inducible ACCD encoding gene AcdS is differentially regulated by varying environmental conditions. Successful generation of transgenic plants with microbial AcdS gene enhanced biotic and abiotic stress tolerance in plants. In the present review, we discuss the importance of ACCD-producing PGPB for their ability to reduce ethylene production and the promotion of plant growth under stress conditions. We also highlighted the development of transgenic plants by overexpressing bacterial AcdS gene to improve their performance under stress conditions.