Comparative mineral and biochemical characterization of Citrus reticulata fruits and leaves to citrus canker pathogens, Xanthomonas axonopodis.

Pakistan's economy greatly benefits from citrus production since these fruits are sold and consumed all over the world. Although citrus fruits are easy to cultivate, they are susceptible to diseases caused by bacteria, viruses, and fungi. These challenges, as well as difficulties in obtaining the proper nutrients, might negatively impact fruit yields and quality. Citrus canker is another complicated problem caused by the germ Xanthomonas axonopodis. This germ affects many types of citrus fruits all over the world. This study looked closely at how citrus canker affects the leaves and the quality of the fruit in places like Sargodha, Bhalwal, Kotmomin, and Silanwali, which are big areas for growing citrus in the Sargodha district. What we found was that plants without the disease had more chlorophyll in their leaves compared to the sick plants. Also, the healthy plants had better amounts of important minerals like calcium, magnesium, potassium, and phosphorus in their fruits. But the fruits with the disease had too much sodium, and the iron levels were a bit different. The fruits with the disease also didn't have as much of something that protects them called antioxidants, which made them more likely to get sick. This study helps us understand how citrus canker affects plants and fruit, so we can think of ways to deal with it.

Restoration of Antibacterial Activity of Inactive Antibiotics via Combined Treatment with AgNPs.

Antimicrobial resistance poses a huge threat to human health around the world and calls for novel treatments. Combined formulations of NPs and antibiotics have emerged as a viable nanoplatform for combating bacterial resistance. The present research work was performed to investigate the effect of combined formulations of AgNPs with streptomycin, cefaclor, ciprofloxacin, and trimethoprim against multidrug-resistant (MDR) isolates of Staphylococcus aureus and Klebsiella pneumoniae. AgNPs have been synthesized by using the Nigella sativa seed extract, and their characteristics were analyzed. AgNPs depicted concentration-dependent antibacterial effects, as the highest concentration of AgNPs showed the strongest antibacterial activity. Interestingly, AgNPs in conjugation with antibiotics showed an enhanced antibacterial potential against both S. aureus and K. pneumoniae, which suggested synergism between the AgNPs and antibiotics. Against S. aureus, streptomycin and trimethoprim in conjugation with AgNPs presented a synergistic effect, while cefaclor and ciprofloxacin in combination with AgNPs showed an additive effect. However, all of the tested antibiotics depicted a synergistic effect against K. pneumoniae. The lowest value of MIC (0.78 µg/mL) was shown by AgNPs-Stp against S. aureus, whereas AgNPs-Tmp showed the lowest value of MIC (1.56 µg/mL) against K. pneumoniae. The most important point of the present study is that both organisms (S. aureus and K. pneumoniae) showed resistance to antibiotics but turned out to be highly susceptible when the same antibiotic was used in combination with AgNPs. These findings highlight the potential of nanoconjugates (the AgNPs-antibiotic complex) to mitigate the present-day crisis of antibiotic resistance and to combat antimicrobial infections efficiently.

Zinc oxide nanoparticles and Klebsiella sp. SBP-8 alleviates chromium toxicity in Brassica juncea by regulation of antioxidant capacity, osmolyte production, nutritional content and reduction in chromium adsorption.

Heavy metals are one of the most damaging environmental toxins that hamper growth of plants. These noxious chemicals include lead (Pb), arsenic (As), nickel (Ni), cadmium (Cd) and chromium (Cr). Chromium is one of the toxic metal which induces various oxidative processes in plants. The emerging role of nanoparticles as pesticides, fertilizers and growth regulators have attracted the attention of various scientists. Current study was conducted to explore the potential of zinc oxide nanoparticles (ZnONPs) alone and in combination with plant growth promoting rhizobacteria (PGPR) Klebsiella sp. SBP-8 in Cr stress alleviation in Brassica juncea (L.). Chromium stress reduced shoot fresh weight (40%), root fresh weight (28%), shoot dry weight (28%) and root dry weight (34%) in B. juncea seedlings. Chromium stressed B. juncea plants showed enhanced levels of malondialdehyde (MDA), electrolyte leakage (EL), hydrogen peroxide (H2O2) and superoxide ion (O2• -). However, co-supplementation of ZnONPs and Klebsiella sp. SBP-8 escalated the activity of antioxidant enzymes i.e., superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) in B. juncea grown in normal and Cr-toxic soil. It is further proposed that combined treatment of ZnONPs and Klebsiella sp. SBP-8 may be useful for alleviation of other abiotic stresses in plants.

The Glycine soja cytochrome P450 gene GsCYP82C4 confers alkaline tolerance by promoting reactive oxygen species scavenging.

Recent studies have demonstrated the crucial role of Cytochrome P450 enzymes (CYPs) in the production of secondary metabolites, phytohormones and antioxidants in plants. However, their functional characterization specifically under alkaline stress remains elusive. CYP82C4 was the key gene screened from a family of wild soybean CYPs in our previous studies. The aim of this present study was to clone the Glycine soja GsCYP82C4 gene and characterize its functions in Arabidopsis and Glycine max. The results showed that the GsCYP82C4 gene displayed a high expression in different plant tissues at mature stages compared to young stages. Further, higher temporal expression of the GsCYP82C4 gene was noted at 6, 12 and 24 h time points after alkali treatment in leaves compared to roots. In addition, overexpression of GsCYP82C4 improved alkaline stress tolerance in Arabidopsis via increased root lengths and fresh biomass and strengthened the antioxidant defense system via a reduction in superoxide radicals in transgenic lines compared to wild type (WT) and atcyp82c4 mutants. Further, the expression levels of stress-related marker genes were up-regulated in GsCYP82C4 OX lines under alkali stress. The functional analysis of GsCYP82C4 overexpression in soybean displayed better hairy root growth, increased fresh weight, higher antioxidant enzyme activities and reduced lipid peroxidation rates in OX lines compared to the soybean WT (K599) line. In total, our study displayed positive roles of GsCYP82C4 overexpression in both Arabidopsis and Glycine max to alleviate alkaline stress via altering expression abundance of stress responsive genes, stronger roots, higher antioxidant enzyme activities as well as reduced rates of lipid peroxidation and superoxide radicals.

Maize/peanut rotation intercropping improves ecosystem carbon budget and economic benefits in the dry farming regions of China.

Monoculture is widely practiced to increase crop productivity, but long-term adaptation has drawbacks as it increases the depletion of soil nutrients and reduces soil quality, especially in dryland areas. Conversion from traditional maize monoculture to intercropping improves sustainable production. However, maize/peanut intercropping, especially rotation of planting strips impacts of maize/peanut intercropping in dryland on carbon (C) budgets and economic benefits remain unclear. In this study, a 5-year field experiment was conducted to evaluate the influence of maize/peanut intercropping with rotation of planting strips on soil health, indirect CO2-eq greenhouse gas emissions, and ecosystem C inputs. Four intercropping treatments viz. maize monoculture, peanut monoculture, maize/peanut intercropping, and maize/peanut rotation-intercropping were tested from 2018 to 2022. Maize/peanut rotation intercropping significantly improved the land equivalent ratio followed by intercropping and monoculture. Rotation-intercropping also improved economic benefits over intercropping and monoculture which were mainly associated with increased peanut yield where the border rows contributed the maximum, followed by the middle rows. Moreover, rotation-intercropping significantly increased the soil organic C and nitrogen (N) content. Rotation-intercropping decreased indirect CO2-eq greenhouse gas emissions and ecosystem C inputs by 3.11% and 18.04%, whereas increased ecosystem C outputs and net ecosystem C budget by 10.38% and 29.14%, respectively, over the average of monoculture. On average for intercropping and monoculture, rotation-intercropping increased ecosystem C emission efficiency for economic benefits by 51.94% and 227.27% in 2021 and 2022, respectively, showing the highest C utilization efficiency than other treatments. In the long run, maize/peanut rotation-intercropping can be practiced in dryland agriculture to achieve sustainable agriculture goals.

Magnesium nanoparticles extirpate salt stress in carrots (Daucus carota L.) through metabolomics regulations.

Underground vegetables are sensitive and vulnerable to salt stress. The vegetables are the main source of vitamins, nutrients and minerals in human diet. Also contain healthy carbohydrates, antioxidant and resistant starch which are beneficial for human health. Salinity influences water balance, morphological appearance and cellular interference of crop plants. It also caused disproportion of nutrients which usually affects the physiochemical processes in plant. Salt stress also affect biochemical attributes and hampers the growth of underground organs, due to which yield of crop decreased. The nanoparticles had been potentially used for better crop yield, in the recent. In our research study, we elaborate the positive response of magnesium oxide nanoparticles (MgO-NPs) on the morphological and biochemical parameters as well as anti-oxidant enzymes action on two accessions of carrot (Daucus carota L.) under salt stress of 40 mM and 80 mM. In a pilot experiment, various levels (0, 50, 100, 150, 200 and 250 mg/L) of MgO-NPs were tested through foliar application on carrot plants. Foliar application of MgO-NPs at concentration of 150 mg/L was most effective treatment and ameliorate the salt stress in both carrot accessions (DC-03 and DC-90). The MgO-NPs significantly enhanced the morphological and biochemical parameters. The yield was significantly increased with the exposure of MgO-NPs. Our results thus confirmed the potential of MgO-NPs to endorse the plant development and growth under salinity. However, further research study is needed to explore effectiveness of MgO-NPs in various other plants for the ameliorant of salinity.

Layer by layer application of chitosan and carboxymethyl cellulose coatings delays ripening of mango fruit by suppressing cell wall polysaccharides disassembly.

Mango is a climacteric fruit that ripens quickly after harvest due to its climacteric nature. Edible coatings have been reported to delay the ripening of various harvested fruit. The efficacy of the applied edible coatings could be improved by using in combination as a layer-by-layer (LBL) approach. So, the influence of LBL application of chitosan (CH) and carboxymethyl cellulose (CMC) was studied on mangoes during postharvest storage at 15 °C for 20 days. Mangoes were coated with monolayers of CH (1 % w/v) and CMC (1 % w/v) as well as LBL application of CH and CMC and were compared with control. The treatment of mangoes with CH and CMC-based LBL treatment resulted in lower decay percentage and weight loss along with higher total chlorophyll pigments and suppressed total carotenoid accumulation. The LBL application of CH and CMC showed lower activity of chlorophyll degrading such as chlorophyllase (CPS), pheophytinase (Phe), Mg-dechalatase (MGD) and chlorophyll degrading peroxidase (Chl-POD) enzymes as well as reduced changes in b*, a* and L* along with a suppressed increase in ethylene (EP) and CO2 production (CPR) rates having higher antioxidant such as catalase (CAT), ascorbate peroxidase (APX), peroxidase (POD) and superoxide dismutase (SOD) enzymes activity. In addition, mangoes coated with LBL treatment of CH and CMC exhibited lower water-soluble pectin (WSP) and higher protopectin (PP) having higher concentrations of chelate soluble (CSP) and sodium carbonate-soluble pectin (SCP). Similarly, LBL-coated mangoes showed significantly higher hemicellulose (HCLS) and cellulose (CLS) contents in contrast with control. It was observed that mangoes coated with CH and CMC-based LBL coating exhibited higher flesh firmness and showed suppressed cellulase (CS), pectin methylesterase (PME), polygalacturonase (PG) and ß-galactosidase (ß-Gal) enzymes activity. The concentrations of total soluble solids and ripening index were markedly lower and titratable acidity was higher in the LBL-based coating treatment in comparison with control. In conclusion, LBL treatment based on CH and CMC coatings could be used for the delay of ripening and softening of harvested mangoes.

Calcium oxide nanoparticles mitigate lead stress in Abelmoschus esculentus though improving the key antioxidative enzymes, nutritional content and modulation of stress markers.

Lead (Pb) is thought to be one of most injurious metals on the earth. Lead stress in plants enhances synthesis of highly toxic reactive oxygen species (ROS). During present research, impact of calcium-oxide nanoparticles (CaO-NPs) was observed on antioxidative defense mechanism in Abelmoschus esculentus plants prone to Pb stress. A CRD experiment was employed with 5 replicates having four treatments (T0 = Control, T1 = Pb stress (200 ppm), T2 = CaO-NPs and T3 = Pb + CaO-NPs). Pb-stressed seedlings exhibited decreased root growth, shoot growth, chlorophyll concentration and biomass accumulation. Moreover, higher synthesis of hydrogen-peroxide (H2O2), malondialdehyde (MDA) and electrolyte leakage (EL) resulting in cellular injuries were noted in plants growing in Pb spiked conditions. Similarly, stressed plants showed higher accumulation of total soluble sugar and proline content besides elevated activity of antioxidative enzymes counting catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR) and ascorbate peroxidase (APX). On the contrary side, CaO-NPs alleviated the Pb induced phytotoxicity through improving activity of antioxidative enzymes. The elevated activity of antioxidant enzymes reduced biosynthesis of H2O2 and MDA which was revealed through the increased growth parameters. In addition, CaO-NPs persuaded enhancement in plant defence machinery by decreased chlorophyll deprivation and augmented the uptake of plant nutrients including K and Ca content. Hence, CaO-NPs can be potent regulators of the antioxidative enzymes and stress markers to ameliorate abiotic stresses.

Mitigation of chromium-induced phytotoxicity in 28-homobrassinolide treated Trigonella corniculata L. by modulation of oxidative biomarkers and antioxidant system.

Chromium (Cr) is one of the toxic heavy metals that disturbs growth and physiological properties of plants. During the current study, Trigonella corniculata L. (Fenugreek) was exposed to different levels of Cr in potted soil. Chromium toxicity reduced fiber, ash, moisture, carbohydrate, protein, fats, and flavonoid content of T. corniculata. Considering the stress relieving effect of 28-homobrassinolide (28-HBR), seeds of T. corniculata were primed with different concentration of 28-HBR i.e., 0, 5, 10, and 20 µmol L-1. Application of 28-HBR reversed the toxic effect of Cr through improvement in activity of antioxidant enzymes like superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT). Conclusively, 10 µmol L-1 28-HBR increased Cr tolerance in T. corniculata seedlings due to reduction in oxidative stress markers. It is further proposed that 28-HBR is an effective stress ameliorant to relive plants from various abiotic stresses.

Stress mitigation by riparian flora in industrial contaminated area of River Chenab Punjab, Pakistan.

Faisalabad is a major industrial area in Pakistan's Punjab province that discharges wastewater into the Chenab River. Industrial effluents in Faisalabad are predicted to pose a significant threat to the riparian vegetation of the Chenab River and nearby vegetation. Heavy metal pollution of plants, water, and soils is one of the biggest problems worldwide that needs to be addressed because heavy metals above normal levels are extremely dangerous to both riparian vegetation and wildlife. The results indicated high levels of pollution in the industrial effluents as well as in the river in terms of salinity, metal toxicity, TSS, TDS, SAR, the acidic and alkaline nature of the industrial effluents, and the spread of industrial effluents up to 15 square kilometres in the Chenab River. Despite the higher pollution, four plants were found at all sites: Calotropis procera, Phyla nodiflora, Eclipta alba and Ranunculus sceleratus. It was found that most of the selected plants were phytoaccumulators, making them best suited to survive in harsh environments such as those with industrial pollution. The Fe concentration in the plant constituents was the highest, along with Zn, Pb, Cd, and Cu, all of which were above the permissible limits of the WHO. The metal transfer factor (MTF) was higher in most of the plants studied, and even exceeded 10 at some severely affected sites. Calotropis procera proved to be the most suitable plant for growth on drainage systems and also at river sites, as it had the highest importance value across all sites and seasons.

Synergistic Impacts of Plant-Growth-Promoting Bacteria and Selenium Nanoparticles on Improving the Nutritional Value and Biological Activities of Three Cultivars of Brassica Sprouts.

Due to the growing world population and increasing environmental stress, improving the production, nutritional quality, and pharmaceutical applications of plants have become an urgent need. Therefore, current research was designed to investigate the impact of seed priming using plant-growth-promoting bacteria (PGPB) along with selenium nanoparticles (SeNPs) treatment on chemical and biological properties of three Brassica oleracea cultivars [Southern star (VA1), Prominence (VA2), Monotop (VA3)]. With this aim, one out of five morphologically different strains of bacteria, namely, JM18, which was further identified via 16S rRNA gene sequencing as a Nocardiopsis species with strong plant-growth-promoting traits, isolated from soil, was used. To explore the growth-promoting potential of Nocardiopsis species, seeds of three varieties of B. oleracea were primed with JM18 individually or in combination with SeNP treatment. Seed treatments increased sprout growth (fresh and dry weights) and glucosinolate accumulation. The activity of myrosinase was significantly increased through brassica sprouts and consequently enhanced the amino-acid-derived glucosinolate induction. Notably, a reduction in effective sulforaphane nitrile was detected, being positively correlated with a decrease in epithiospecifier protein (EP). Consequently, the antioxidant activities of VA2 and VA3, determined by the ferric reducing antioxidant power (FRAP) assay, were increased by 74 and 79%, respectively. Additionally, the antibacterial activities of JM18-treated cultivars were improved. However, a decrease was observed in SeNP- and JM18 + SeNP-treated VA2 and VA3 against Serratia marcescens and Candida glabrata and VA1 against S. marcescens. In conclusion, seed priming with the JM18 extract is a promising method to enhance the health-promoting activities of B. oleracea sprouts.

Rice straw based silicon nanoparticles improve morphological and nutrient profile of rice plants under salinity stress by triggering physiological and genetic repair mechanisms.

The agricultural sector is facing numerous challenges worldwide, owing to global climate change and limited resources. Crop production is limited by numerous abiotic constraints. Among them, salinity stress as a combination of osmotic and ionic stress adversely influences the physiological and biochemical processes of the plant. Nanotechnology facilitates the production of crops either directly by eradicating the losses due to challenging environmental conditions or indirectly by improving tolerance against salinity stress. In this study, the protective role of silicon nanoparticles (SiNPs) was determined in two rice genotypes, N-22 and Super-Bas, differing in salinity tolerance. The SiNPs were confirmed through standard material characterization techniques, which showed the production of spherical-shaped crystalline SiNPs with a size in the range of 14.98-23.74 nm, respectively. Salinity stress adversely affected the morphological and physiological parameters of both varieties, with Super-Bas being more affected. Salt stress disturbed the ionic balance by minimizing the uptake of K+ and Ca2+ contents and increased the uptake of Na+ in plants. Exogenous SiNPs alleviated the toxic effects of salt stress and promoted the growth of both N-22 and Super-Bas, chlorophyll contents (16% and 13%), carotenoids (15% and 11%), total soluble protein contents (21% and 18%), and the activities of antioxidant enzymes. Expression analysis from quantitative real-time PCR showed that SiNPs relieved plants from oxidative bursts by triggering the expression of HKT genes. Overall, these findings demonstrate that SiNPs significantly alleviated salinity stress by triggering physiological and genetic repair mechanisms, offering a potential solution for food security.

Long-Term Nutrient Cycle in Improved Grain Yield of Dryland Winter Wheat (Triticum aestivum L.) under Hydrological Process of Plant Ecosystem Distribution in the Loess Plateau of China.

Precipitation is the major cause of crop yield variation in rainfed agriculture production in the Loess Plateau. As over fertilization is economically and environmentally undesirable, and crop yield and the resulting returns for N input are uncertain when rainfall variability is high, optimizing N management according to precipitation during fallow season is vital for efficient crop water use and high yield in dryland rainfed farming systems. Results show that the nitrogen treatment rate of 180 treatment significantly increased the tiller percentage rate, and the leaf area index at anthesis, the jointing anthesis, anthesis maturity dry matter, and nitrogen accumulation was closely related to yield. N150 treatment compared to N180 treatment significantly increased the percentage of ear-bearing tiller by 7%, dry substance accretion from jointing to anthesis by 9%, and yield by 17% and 15%, respectively. Our study has important implications for the assessment of the effects of fallow precipitation, as well as for the sustainable development of dryland agriculture in the Loess Plateau. Our results indicate that adjusting N fertilizer inputs based on summer rainfall variation could enhance wheat yield in rainfed farming systems.

Effect of nanobiochar (nBC) on morpho-physio-biochemical responses of black cumin (Nigella sativa L.) in Cr-spiked soil.

Chromium is a highly toxic heavy metal. High concentrations of Cr (III) can affect metabolic processes in plants, resulting in different morphological, physiological, and biochemical defects. Agricultural practices such as sewage irrigation, over-fertilization, and sewage sludge application contribute significantly to Cr contamination. It can reduce the growth of plants by affecting the activity of antioxidant enzymes. The materials in nano form play an important role in nano-remediation and heavy metals absorption due to their high surface area and micropores. This research was conducted to study the potential of foliar application of nanobiochar/nBC (100 mg/L-1 and 150 mg/L-1) for mitigation of Cr (III) stress (200 mg/kg and 300 mg/kg) in black cumin (Nigella sativa) plants. The results showed that increased Cr stress (300 mg/kg) decreased the plant growth parameters, chlorophyll content, total soluble sugars, and proteins. However, increased the level of hydrogen peroxide (H2O2) and malondialdehyde acetate (MDA) as a result of the activity of antioxidant enzymes (Catalase, Superoxide dismutase, peroxidase dismutase, and ascorbic peroxidase) increased in Nigella sativa seedlings. Foliar application of the nBC (100 mg/L-1) increased plant growth parameters, chlorophyll content, and osmoprotectants, while decreasing the levels of oxidative stress markers (H2O2 and MDA). Furthermore, with the application of nBC, the antioxidant enzyme activity considerably improved. Improved antioxidant activity shows that nBC helped to decrease oxidative stress, which in return improved the growth of Nigella sativa seedlings. Overall, present study findings concluded that foliar application of nBC in Nigella sativa seedlings improved growth, chlorophyll, and antioxidant enzymes. The nBC treatment of 100 mg/L-1 showed better results compared to 150 mg/L-1 under chromium stress.

The combined application of Arabic gum coating and γ-aminobutyric acid mitigates chilling injury and maintains eating quality of 'Kinnow' mandarin fruits.

Low temperature storage of citrus generally extends the storage potential but leads to chilling injury appearance on the rind of fruits. The said physiological disorder has been found associated with changes in the metabolism of cell walls and other attributes. In this work, the influence of Arabic gum [AG (10 %)] and γ-amminobutyric acid [GABA (10 mmol L-1)] either alone or in combined form was studied on fruits of 'Kinnow' mandarin during storage at 5 ± 1 °C for 60 days. The results exhibited that the combined AG + GABA treatment significantly suppressed weight loss (5.13 %), chilling injury (CI) symptoms (2.41 score), incidence of disease (13.33 %), respiration rate [(4.81 µmol kg-1 h-1) RPR] and ethylene production [(0.86 nmol kg-1 h-1) EPR]. In addition, AG + GABA application reduced relative electrolyte (37.89 %) leakage (REL), malondialdehyde [(25.99 nmol kg-1) MDA], superoxide anion [(15.23 nmol min-1 kg-1) O2•-] and hydrogen peroxide [(27.08 nmol kg-1) H2O2] along with lower lipoxygenase [(23.81 U mg-1 protein) LOX] and phospholipase D [(14.07 U mg-1 protein) PLD] enzyme activities compared with control. The AG + GABA treated 'Kinnow' group showed higher glutamate decarboxylase [(43.18 U mg-1 protein) GAD] and lower GABA transaminase [(15.93 U mg-1 protein) GABA-T] activity having higher endogenous GABA (42.02 mg kg-1) content. The fruits treated with AG + GABA exhibited higher cell walls (CW) components such as Na2CO3-soluble pectin [(6.55 g kg-1) NCSP], chelate-soluble pectin [(7.13 g kg-1) CSP] and protopectin [(11.03 g kg-1) PRP] concentrations along with lower water-soluble pectin [(10.64 g kg-1) WSP] compared to control. In addition, 'Kinnow' fruits treated with AG + GABA showed higher firmness (8.63 N) and lower activities of CW degrading such as cellulase [(11.23 U mg-1 protein) CX], polygalacturonase [(22.59 U mg-1 protein) PG], pectin methylesterase [(15.61 U mg-1 protein) PME] and ß-galactosidase [(20.64 U mg-1 protein) ß-Gal] enzymes. The activity of catalase [(41.56 U mg-1 protein) CAT], ascorbate peroxidase [(55.57 U mg-1 protein) APX], superoxide dismutase [(52.93 U mg-1 protein) SOD] and peroxidase [(31.02 U mg-1 protein) POD] was also higher in combined treatment. In addition, AG + GABA treated fruits showed better biochemical and sensory attributes than the control. So, combined AG + GABA could be used for CI mitigation and storage life prolongation of 'Kinnow' fruits.

Synergistic soil-less medium for enhanced yield of crops: a step towards incorporating genomic tools for attaining net zero hunger.

Globally, industrial farming endangers crucial ecological mechanisms upon which food production relies, while 815 million people are undernourished and a significant number are malnourished. Zero Hunger aims to concurrently solve global ecological sustainability and food security concerns. Recent breakthroughs in molecular tools and approaches have allowed scientists to detect and comprehend the nature and structure of agro-biodiversity at the molecular and genetic levels, providing us an advantage over traditional methods of crop breeding. These bioinformatics techniques let us optimize our target plants for our soil-less medium and vice versa. Most of the soil-borne and seed-borne diseases are the outcomes of non-treated seed and growth media, which are important factors in low productivity. The farmers do not consider these issues, thereby facing problems growing healthy crops and suffering economic losses. This study is going to help the farmers increase their eco-friendly, chemical residue-free, quality yield of crops and their economic returns. The present invention discloses a synergistic soil-less medium that consists of only four ingredients mixed in optimal ratios by weight: vermicompost (70-80%), vermiculite (10-15%), coco peat (10-15%), and Rhizobium (0-1%). The medium exhibits better physical and chemical characteristics than existing conventional media. The vermiculite to coco peat ratio is reduced, while the vermicompost ratio is increased, with the goals of lowering toxicity, increasing plant and water holding capacity, avoiding drying of the media, and conserving water. The medium provides balanced nutrition and proper ventilation for seed germination and the growth of seedlings. Rhizobium is also used to treat the plastic bags and seeds. The results clearly show that the current synergistic soil-less environment is best for complete plant growth. Securing genetic advantages via sexual recombination, induced random mutations, and transgenic techniques have been essential for the development of improved agricultural varieties. The recent availability of targeted genome-editing technology provides a new path for integrating beneficial genetic modifications into the most significant agricultural species on the planet. Clustered regularly interspaced short palindromic repeats and associated protein 9 (CRISPR/Cas9) has evolved into a potent genome-editing tool for imparting genetic modifications to crop species. In addition, the integration of analytical methods like population genomics, phylogenomics, and metagenomics addresses conservation problems, while whole genome sequencing has opened up a new dimension for explaining the genome architecture and its interactions with other species. The in silico genomic and proteomic investigation was also conducted to forecast future investigations for the growth of French beans on a synergistic soil-less medium with the purpose of studying how a blend of vermicompost, vermiculite, cocopeat, and Rhizobium secrete metal ions, and other chemical compounds into the soil-less medium and affect the development of our target plant as well as several other plants. This interaction was studied using functional and conserved region analysis, phylogenetic analysis, and docking tools.

Host-mediated gene engineering and microbiome-based technology optimization for sustainable agriculture and environment.

The agricultural sector and environmental safety both work hand in hand to promote sustainability in important issues like soil health, plant nutrition, food safety, and security. The conventional methods have greatly harmed the environment and people's health and caused soil fertility and quality to decline as well as deteriorate. Keeping in view the excessive exploitation and cascade of degradation events due to unsustainable farming practices, the need of the hour demands choosing an appropriate, eco-friendly strategy to restore soil health, plant nutrition, and environmental aspects. The priority highlights a need for a sustainable and environment-friendly upgradation of the present agricultural systems to utilize the beneficial aspects related to harnessing the gene-microbiome strategies which would help in the restoration and replenishment of the microbial pool. Thus, exploring the microbiome is the utmost priority which gives a deep insight into the different aspects related to soil and plant and stands out as an important contributor to plant health and productivity. "Microbes" are important drivers for the biogeochemical cycles and targets like sustainability and safety. This essential microbial bulk (soil microbiome) is greatly influenced by agricultural/farming practices. Therefore, with the help of microbiome engineering technologies like meta-transcriptomics, meta-proteomics, metabolomics, and novel gene-altering techniques, we can easily screen out the highly diverse and balanced microbial population in the bulk of soil, enhancing the soil's health and productivity. Importantly, we need to change our cultivation strategies to attain such sustainability. There is an urgent need to revert to natural/organic systems of cultivation patterns where the microbiome hub can be properly utilized to strengthen soil health, decrease insect pest and disease incidence, reduce greenhouse gas emissions, and ultimately prevent environmental degradation. Through this article, we wish to propose a shift in the cultivation pattern from chemical to the novel, upgraded gene-assisted designed eco-friendly methodologies which can help in incorporating, exploring, and harnessing the right microbiome consortium and can further help in the progression of environmentally friendly microbiome technologies for agricultural safety and productivity.

Triticum aestivum: antioxidant gene profiling and morpho-physiological studies under salt stress.

BACKGROUND: Soil salinity drastically reduced wheat growth and production in Pakistan. It is a need of an hour to identify the best suitable salt tolerance or resistant wheat varieties which shows good growth under salinity affected areas. In presented study, two wheat varieties Johar (salt tolerant) and Sarsabaz (salt sensitive) were examined under NaCl stress conditions. METHODS: Antioxidant enzyme activities were investigated in 10-days old wheat seedlings under 200 mM NaCl stress in hydroponic conditions. To investigate the various growth parameters, antioxidant enzyme activities such as superoxide dismutase (SOD: EC 1.15.1.1), catalase (CAT: EC 1.11.1.6) and ascorbate peroxidase (APX: EC 1.11.1.11) were monitored and studied. Besides this various growth parameters such as length of the roots, shoots, as well as Physiological parameters likes lipid peroxidation by malondialdehyde (MDA), hydrogen peroxide (H2O2), and proline contents and antioxidant enzyme activities were estimated. The effect of salinity was also observed on gene transcription level and eventually expression level. RESULTS: Shoot and root length were decreased in Sarsabaz variety while it showed opposite trend in johar at 200 mM salt concentration. The concentration of proline showed a noticeable rise in salt dependency. Higher concentrations of Proline in Johar were observed as compared to Sarsabaz. SOD showed the increase in activity for antioxidant enzymes. Significant increase of SOD levels were observed in shoot tissues as compared to root tissues. The results indicated that the shoots were more susceptible to salt stress. Activity of APX showed similar affects in both varieties. The production of CAT enzyme in the shoot and root tissues of both varieties showed substantial growth under increased salt stress. Furthermore, NaCl stress has increased the expression of certain genes coding for antioxidant enzymes such as catalase, superoxide dismutase, and peroxidase. Maximum expression of all the antioxidant enzyme coding genes were observed in Johar (tolerant) at 48 h exposure to salt. In contrast the expression of the all mentioned genes in Sarsabaz variety were found maximum at early hours (24 h) and gradually decreased at 48 h. CONCLUSION: The study showed that the selected salt tolerant wheat variety Johar is significantly resistant to 200 mM NaCl salt level as compared to Sarsabaz.

Synthesis of phytostabilized zinc oxide nanoparticles and their effects on physiological and anti-oxidative responses of Zea mays (L.) under chromium stress.

Chromium (Cr) is a hazardous metal that has a significant risk of transfer from soil to edible parts of food crops, including shoot tissues. Reduction of Cr accumulation is required to lower the risk of Cr-exposed in humans and animals feeding on metal-contaminated parts of such plant. Zea mays is a global staple crop irrigated intensively with Cr-contaminated water. Consequently, the objective of this study was to investigate that FI-stabilized ZnO NPs could be used as an eco-friendly and efficient amendment to reduced Cr uptake and toxicity in Zea mays. To investigate the growth parameters, physiological, oxidative stress and biochemical parameters under different Cr-VI concentrations (10.0, 15.0, and 20.0 ppm). Cr exposed Z. mays plants exhibited substantially reduced plant biomass, chlorophyll contents, and altered antioxidant enzyme activity compared to untreated control. The results revealed that foliar application of Fagonia-ZnO-NPs helps eliminate the harmful effects of Cr (VI), which can enter plants through soil pollution. Increased levels of proline, soluble sugars and various antioxidant enzymes reflected this. Mean comparisons showed that Cr stress led to a 33-50% reduction in fresh shoot weight, 73-170% in fresh root weight, 16-34% shoot length, 9.5-129% root length, Chlorophyll contents 20-33% (Chl a), 18-27% (Chl b) and 17-27% (car), 14-33% total soluble sugars, 54-170% proline content, 7-7.5% POD, 0.66-75% CAT and 32-77% APX enzyme activities compared to untreated plants. Application of FI-stabilized ZnO NPs led to an increase 21-77% in fresh shoot weight, 22-45%, fresh root weight, 3-35% shoot length, 24-154% root length, Chlorophyll contents 39-60% (Chl a), 15-79% (Chl b) and 28-82% (car), 19-52% total soluble sugars, 21-55% proline content, 14-43% POD, 34-95% CAT and 130-186% APX enzyme activities under 10, 15 and 20 ppm Cr stress respectively, compared to Cr-treated plants. However, the principal component analysis revealed that chlorophyll contents, carotenoid, CAT, APX and length were in the same group and showed a positive correlation. These data collectively suggest that phytostabilized zinc oxide NPs may be an eco-friendly solution to mitigate Cr toxicity in agricultural soils and crop plants.