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SUPPRESSOR OF MAX2 LIKE 1 (SMAX1) is a member of the SUPPRESSOR of MAX2 1LIKE family of genes and is known as a target protein of KARRIKIN INSENSITIVE2 (KAI2)-MORE AXILLARY BRANCHES2 (MAX2), which mediates karrikin signaling in Arabidopsis. SMAX1 plays a significant role in seed germination, hypocotyl elongation, and root hair development in Arabidopsis. SMAX1 has not yet been identified and characterized in woody plants. This study identified and characterized SsSMAX1 in Sapium sebiferum and found that SsSMAX1 was highly expressed in the seed, hypocotyl, and root tips of S. sebiferum. SsSMAX1 was functionally characterized by ectopic expression in Arabidopsis. SsSMAX1 overexpression lines of Arabidopsis showed significantly delayed seed germination and produced seedlings with longer hypocotyl and roots than wild-type and Atsmax1 functional mutants. SsSMAX1 overexpression lines of Arabidopsis also had broader and longer leaves and petioles than wild-type and Atsmax1, suggesting that SsSMAX1 is functionally conserved. This study characterizes the SMAX1 gene in a woody and commercially valuable bioenergy plant, Sapium sebiferum. The results of this study are beneficial to future research on the molecular biology of woody plants.
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Proteínas de Arabidopsis , Arabidopsis , Arabidopsis/genética , Proteínas de Arabidopsis/genética , Furanos/metabolismo , Peptídeos e Proteínas de Sinalização Intracelular/metabolismoRESUMO
Acer palmatum (A. palmatum), a deciduous shrub or small arbour which belongs to Acer of Aceraceae, is an excellent greening species as well as a beautiful ornamental plant. In this study, a high-quality chromosome-level reference genome for A. palmatum was constructed using Oxford Nanopore sequencing and Hi-C technology. The assembly genome was â¼745.78 Mb long with a contig N50 length of 3.20 Mb, and 95.30 % (710.71 Mb) of the assembly was anchored into 13 pseudochromosomes. A total of 28,559 protein-coding genes were obtained, â¼90.02 % (25,710) of which could be functionally annotated. The genomic evolutionary analysis revealed that A. palmatum is most closely related to A. yangbiense and A. truncatum, and underwent only an ancient gamma whole-genome duplication event. Despite lacking a recent independent WGD, 25,795 (90.32 %) genes of A. palmatum were duplicated, and the unique/expanded gene families were linked with genes involved in plant-pathogen interaction and several metabolic pathways, which might underpin adaptability. A combined genomic, transcriptomic, and metabolomic analysis related to the biosynthesis of anthocyanin in leaves during the different season were characterized. The results indicate that the dark-purple colouration of the leaves in spring was caused by a high amount of anthocyanins, especially delphinidin and its derivatives; and the red colouration of the leaves in autumn by a high amount of cyanidin 3-O-glucoside. In conclusion, these valuable multi-omic resources offer important foundations to explore the molecular regulation mechanism in leaf colouration and also provide a platform for the scientific and efficient utilization of A. palmatum.
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Acer , Acer/genética , Antocianinas/genética , Multiômica , Anotação de Sequência Molecular , Cromossomos , Pigmentação/genética , Folhas de Planta/genéticaRESUMO
In this project, a synergistic approach has been proposed where a quick, easy, cheap, effective, rugged, and safe (QuEChERS) sample preparation technique was developed for the extraction of sudan III and sudan IV dyes in different spices prior to its dispersive liquid-liquid microextraction based on solidified floating organic droplet (DLLME-SFO). Initially, the sample was extracted by QuEChERS method and then preconcentrated through DLLME-SFO followed by spectrophotometric detection. All the experimental parameters i.e., volume of extraction solvent, pH, acetonitrile to water ratio, temperature, centrifugation rate and time, and sample volume were optimized. Limit of detection (LOD) calculated for sudan III and sudan IV were 0.42 and 0.35 mg/L, respectively. Excellent recoveries were obtained in the range of 98.29-99.88%. After validation through standard addition methodology, the developed QuEChERS@DLLME-SFO method was successfully applied to determine sudan (III-IV) dyes in real spices samples. Integration of QuEChERS and DLLME-SFO was found to be a suitable substitute to eliminate the usage of costly primary secondary amines and other sorbents. The synergistic approach of QuEChERS and DLLME-SFO with the aid of UV/visible spectrophotometry makes it prompt, cost effective technique with excellent analytical figures of merit.
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Compostos Azo , Microextração em Fase Líquida/métodos , Compostos Azo/análise , Compostos Azo/química , Compostos Azo/isolamento & purificação , Limite de Detecção , Modelos Lineares , Reprodutibilidade dos Testes , Análise Espectral/métodos , EspeciariasRESUMO
Supramolecular solvent-based dispersive liquid-liquid microextraction technique has been developed as a preconcentration tool for the determination of trace level of Pb2+ and Cd2+. Dodecanol dispersed in tetrahydrofuran has been utilized as a supramolecular-solvent system for the extraction of analytes prior to their quantitative determination with graphite furnace atomic absorption spectrophotometer. Both Pb2+ and Cd2+, which were efficiently extracted by supramolecular solvent system, were complexed with dithizone followed by the addition of supramolecular solvent. The experimental variables that could possibly influence the extraction efficiency, i.e. pH value, temperature, sample volume, centrifugation time, rate of centrifugation, ionic strength, etc. were subjected to the optimization step. An interference study was also conducted to check the selectivity of developed method. Limit of detection calculated for Pb2+ and Cd2+ was 0.015 and 0.061 mg L-1, respectively. The limit of quantification was 0.05 and 0.2 mg L-1 for Pb2+ and Cd2+, respectively. The analytical signal was enhanced to 30 times in case of Pb2+ and 27 times in case of Cd2+. The results obtained revealed that the developed method is rapid, simple, sensitive, and efficient for the determination of both analytes in real water samples.
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Every year, tuberculosis affects the lungs of millions of people and rifampicin is the commonly used medicine for its treatment due to its antibiotic nature. The frequent use of rifampicin may lead to its increased concentration in the water resources. This research work is focused on the cloud point extraction (CPE) procedure for the preconcentration of rifampicin prior to its determination in water. The UV/vis spectrophotometric method was adapted for the measurement of rifampicin content after the phase separation. Triton-X 100 was used as the nonionic surfactant which contains hydrophilic polyethylene chain feasible for the extraction of analyte. Various analytical parameters that can affect the extraction efficacy were optimized to achieve linearity of the proposed method in the concentration range of 3.54-81.41 mgL-1. The Limit of detection and quantification were 1.261 and 4.212 mgL- 1, respectively. The Preconcentration factor was 40 with relative standard deviation (%RSD) of 2.504%. The standard addition methodology was adopted for the validation of this procedure and effectively applied for the determination of rifampicin in real wastewater samples.
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Karrikinolide (KAR1), identified in biochars, has gained research attention because of its significant role in seed germination, seedling development, root development, and abiotic stresses. However, KAR1 regulation of salt stress in wheat is elusive. This study investigated the physiological mechanism involved in KAR1 alleviation of salt stress in wheat. The results showed KAR1 boosted seed germination percentage under salinity stress via stimulating the relative expression of genes regulating gibberellins biosynthesis and decreasing the expression levels of abscisic acid biosynthesis and signaling genes. As seen in seed germination, exogenous supplementation of KAR1 dramatically mitigated the salt stress also in wheat seedling, resulting in increased root and shoot growth as measured in biomass as compared to salt stress alone. Salt stress significantly induced the endogenous hydrogen peroxide and malondialdehyde levels, whereas KAR1 strictly counterbalanced them. Under salt stress, KAR1 supplementation showed significant induction in reduced glutathione (GSH) and reduction in oxidized glutathione (GSSG) content, which improved GSH/GSSG ratio in wheat seedlings. Exogenous supplementation of KAR1 significantly promoted the activities of enzymatic antioxidants in wheat seedlings exposed to salt stress. KAR1 induced the relative expression of genes regulating the biosynthesis of antioxidants in wheat seedlings under salinity. Moreover, KAR1 induced the expression level of K+/Na+ homeostasis genes, reduced Na+ concentration, and induced K+ concentration in wheat seedling under salt stress. The results suggest that KAR1 supplementation maintained the redox and K+/Na+ homeostasis in wheat seedling under salinity, which might be a crucial part of physiological mechanisms in KAR1 induced tolerance to salt stress. In conclusion, we exposed the protective role of KAR1 against salt stress in wheat.
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Germinação , Triticum , Antioxidantes , Furanos , Homeostase , Oxirredução , Piranos , Estresse Salino , Plântula , Estresse Fisiológico , Triticum/genéticaRESUMO
KARRIKINS INSENSITIVE2 (KAI2) is the receptor gene for karrikins, recently found to be involved in seed germination, hypocotyl development, and the alleviation of salinity and osmotic stresses. Nevertheless, whether KAI2 could regulate cold tolerance remains elusive. In the present study, we identified that Arabidopsis mutants of KAI2 had a high mortality rate, while overexpression of, a bioenergy plant, Sapium sebiferum KAI2 (SsKAI2) significantly recovered the plants after cold stress. The results showed that the SsKAI2 overexpression lines (OEs) had significantly increased levels of proline, total soluble sugars, and total soluble protein. Meanwhile, SsKAI2 OEs had a much higher expression of cold-stress-acclimation-relate genes, such as Cold Shock Proteins and C-REPEAT BINDING FACTORS under cold stress. Moreover, the results showed that SsKAI2 OEs were hypersensitive to abscisic acid (ABA), and ABA signaling genes were w significantly affected in SsKAI2 OEs under cold stress, suggesting a potential interaction between SsKAI2 and ABA downstream signaling. In SsKAI2 OEs, the electrolyte leakage, hydrogen peroxide, and malondialdehyde contents were reduced under cold stress in Arabidopsis. SsKAI2 OEs enhanced the anti-oxidants like ascorbate peroxidase, catalase, peroxidase, superoxide dismutase, and total glutathione level under cold stress. Conclusively, these results provide novel insights into the understanding of karrikins role in the regulation of cold stress adaptation.
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Karrikins are reported to stimulate seed germination, regulate seedling growth, and increase the seedling vigor in abiotic stress conditions in plants. Nevertheless, how karrikins alleviate abiotic stress remains largely elusive. In this study, we found that karrikin (KAR1) could significantly alleviate both drought and salt stress in the important oil plant Sapium sebiferum. KAR1 supplementation in growth medium at a nanomolar (nM) concentration was enough to recover seed germination under salt and osmotic stress conditions. One nanomolar of KAR1 improved seedling biomass, increased the taproot length, and increased the number of lateral roots under abiotic stresses, suggesting that KAR1 is a potent alleviator of abiotic stresses in plants. Under abiotic stresses, KAR1-treated seedlings had a higher activity of the key antioxidative enzymes, such as superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase, in comparison with the control, which leads to a lower level of hydrogen peroxide, malondialdehyde, and electrolyte leakage. Moreover, the metabolome analysis showed that KAR1 treatment significantly increased the level of organic acids and amino acids, which played important roles in redox homeostasis under stresses, suggesting that karrikins might alleviate abiotic stresses via the regulation of redox homeostasis. Under abiotic stresses, applications of karrikins did not increase the endogenous abscisic acid level but altered the expression of several ABA signaling genes, such as SNF1-RELATED PROTEIN KINASE2.3, SNF1-RELATED PROTEIN KINASE2.6, ABI3, and ABI5, suggesting potential interactions between karrikins and ABA signaling in the stress responses. Conclusively, we not only provided the physiological and molecular evidence to clarify the mechanism of karrikins in the regulation of stress adaptation in S. sebiferum but also showed the potential value of karrikins in agricultural practices, which will lay a foundation for further studies about the role of karrikins in abiotic stress alleviation in plants.
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Due to a huge increase in polymer production, a tremendous increase in municipal solid waste is observed. Every year the existing landfills for disposal of waste polymers decrease and the effective recycling techniques for waste polymers are getting more and more important. In this work pyrolysis of waste polystyrene was performed in the presence of a laboratory synthesized copper oxide. The samples were pyrolyzed at different heating rates that is, 5°Cmin-1, 10°Cmin-1, 15°Cmin-1 and 20°Cmin-1 in a thermogravimetric analyzer in inert atmosphere using nitrogen. Thermogravimetric data were interpreted using various model fitting (Coats-Redfern) and model free methods (Ozawa-Flynn-Wall, Kissinger-Akahira-Sunose and Friedman). Thermodynamic parameters for the reaction were also determined. The activation energy calculated applying Coats-Redfern, Ozawa-Flynn-Wall, Kissinger-Akahira-Sunose and Friedman models were found in the ranges 105-148.48 kJmol-1, 99.41-140.52 kJmol-1, 103.67-149.15 kJmol-1 and 99.93-141.25 kJmol-1, respectively. The lowest activation energy for polystyrene degradation in the presence of copper oxide indicates the suitability of catalyst for the decomposition reaction to take place at lower temperature. Moreover, the obtained kinetics and thermodynamic parameters would be very helpful in determining the reaction mechanism of the solid waste in a real system.
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Poliestirenos , Resíduos Sólidos , Calefação , Cinética , TermogravimetriaRESUMO
APYRASEs, which directly regulate intra- and extra-cellular ATP homeostasis, play a pivotal role in the regulation of various stress adaptations in mammals, bacteria and plants. In the present study, we identified and characterized wheat APYRASE family members at the genomic level in wheat. The results identified a total of nine APY homologs with conserved ACR domains. The sequence alignments, phylogenetic relations and conserved motifs of wheat APYs were bioinformatically analyzed. Although they share highly conserved secondary and tertiary structures, the wheat APYs could be mainly categorized into three groups, according to phylogenetic and structural analysis. Additionally, these APYs exhibited similar expression patterns in the root and shoot, among which TaAPY3-1, TaAPY3-3 and TaAPY3-4 had the highest expression levels. The time-course expression patterns of the eight APYs in response to biotic and abiotic stress in the wheat seedlings were also investigated. TaAPY3-2, TaAPY3-3, TaAPY3-4 and TaAPY6 exhibited strong sensitivity to all kinds of stresses in the leaves. Some APYs showed specific expression responses, such as TaAPY6 to heavy metal stress, and TaAPY7 to heat and salt stress. These results suggest that the stress-inducible APYs could have potential roles in the regulation of environmental stress adaptations. Moreover, the catalytic activity of TaAPY3-1 was further analyzed in the in vitro system. The results showed that TaAPY3-1 protein exhibited high catalytic activity in the degradation of ATP and ADP, but with low activity in degradation of TTP and GTP. It also has an extensive range of temperature adaptability, but preferred relatively acidic pH conditions. In this study, the genome-wide identification and characterization of APYs in wheat were suggested to be useful for further genetic modifications in the generation of high-stress-tolerant wheat cultivars.
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BACKGROUND: Wheat is one of the most important staple crops worldwide. Fusarium head blight (FHB) severely affects wheat yield and quality. A novel bread wheat mutant, ZK001, characterized as cleistogamic was isolated from a non-cleistogamous variety Yumai 18 (YM18) through static magnetic field mutagenesis. Cleistogamy is a promising strategy for controlling FHB. However, little is known about the mechanism of cleistogamy in wheat. METHODS: We performed a FHB resistance test to identify the FHB infection rate of ZK001. We also measured the agronomic traits of ZK001 and the starch and total soluble sugar contents of lodicules in YM18 and ZK001. Finally, we performed comparative studies at the proteome level between YM18 and ZK001 based on the proteomic technique of isobaric tags for relative and absolute quantification. RESULTS: The infection rate of ZK001 was lower than that of its wild-type and Aikang 58. The abnormal lodicules of ZK001 lost the ability to push the lemma and palea apart during the flowering stage. Proteome analysis showed that the main differentially abundant proteins (DAPs) were related to carbohydrate metabolism, protein transport, and calcium ion binding. These DAPs may work together to regulate cellular homeostasis, osmotic pressure and the development of lodicules. This hypothesis is supported by the analysis of starch, soluble sugar content in the lodicules as well as the results of Quantitative reverse transcription polymerase chain reaction. CONCLUSIONS: Proteomic analysis has provided comprehensive information that should be useful for further research on the lodicule development mechanism in wheat. The ZK001 mutant is optimal for studying flower development in wheat and could be very important for FHB resistant projects via conventional crossing.
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Recent studies have demonstrated that strigolactones (SLs) also participate in the regulation of stress adaptation; however, the regulatory mechanism remains elusive. In this study, the homolog of More Axillary Branches 2, which encodes a key component in SL signaling, in the perennial oil plant Sapium sebiferum was identified and functionally characterized in Arabidopsis. The results showed that the expression of SsMAX2 in S. sebiferum seedlings was stress-responsive, and SsMAX2 overexpression (OE) in Arabidopsis significantly promoted resistance to drought, osmotic, and salt stresses. Moreover, SsMAX2 OE lines exhibited decreased chlorophyll degradation, increased soluble sugar and proline accumulation, and lower water loss ratio in response to the stresses. Importantly, anthocyanin biosynthesis and the activities of several antioxidant enzymes, such as superoxide dismutase (SOD), peroxidase (POD), and ascorbate peroxidase (APX), were enhanced in the SsMAX2 OE lines, which further led to a significant reduction in hydrogen peroxide levels. Additionally, the SsMAX2 OE lines exhibited higher expression level of several abscisic acid (ABA) biosynthesis genes, suggesting potential interactions between SL and ABA in the regulation of stress adaptation. Overall, we provide physiological and biochemical evidence demonstrating the pivotal role of SsMAX2 in the regulation of osmotic, drought, and salt stress resistance and show that MAX2 can be a genetic target to improve stress tolerance.
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Arabidopsis/genética , Arabidopsis/fisiologia , Secas , Homeostase , Proteínas de Plantas/metabolismo , Tolerância ao Sal , Sapium/metabolismo , Estresse Fisiológico , Ácido Abscísico/metabolismo , Antocianinas/metabolismo , Antioxidantes/metabolismo , Arabidopsis/efeitos dos fármacos , Ascorbato Peroxidases/metabolismo , Vias Biossintéticas/genética , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Genes de Plantas , Germinação/efeitos dos fármacos , Homeostase/efeitos dos fármacos , Peróxido de Hidrogênio/metabolismo , Malondialdeído/metabolismo , Modelos Biológicos , Oxirredução , Peroxidase/metabolismo , Filogenia , Proteínas de Plantas/genética , Plantas Geneticamente Modificadas , Prolina/metabolismo , Tolerância ao Sal/efeitos dos fármacos , Sapium/genética , Plântula/efeitos dos fármacos , Plântula/metabolismo , Sementes/efeitos dos fármacos , Sementes/crescimento & desenvolvimento , Cloreto de Sódio/farmacologia , Estresse Fisiológico/efeitos dos fármacos , Estresse Fisiológico/genética , Açúcares/metabolismo , Superóxido Dismutase/metabolismoRESUMO
BACKGROUND: Sapium sebiferum, whose seeds contain high level of fatty acids, has been considered as one of the most important oil plants. However, the high male to female flower ratio limited the seed yield improvement and its industrial potentials. Thus, the study of the sex determination in S. sebiferum is of significant importance in increasing the seed yield. RESULTS: In this study, we demonstrated that in S. sebiferum, cytokinin (CK) had strong feminization effects on the floral development. Exogenous application with 6-benzylaminopurine (6-BA) or thidiazuron (TDZ) significantly induced the development of female flowers and increased the fruit number. Interestingly, the feminization effects of cytokinin were also detected on the androecious genotype of S. sebiferum which only produce male flowers. To further investigate the mechanism underlying the role of cytokinin in the flower development and sex differentiation, we performed the comparative transcriptome analysis of the floral buds of the androecious plants subjected to 6-BA. The results showed that there were separately 129, 352 and 642 genes differentially expressed at 6 h, 12 h and 24 h after 6-BA treatment. Functional analysis of the differentially expressed genes (DEGs) showed that many genes are related to the hormonal biosynthesis and signaling, nutrients translocation and cell cycle. Moreover, there were twenty one flowering-related genes identified to be differentially regulated by 6-BA treatment. Specifically, the gynoecium development-related genes SPATULA (SPT), KANADI 2 (KAN2), JAGGED (JAG) and Cytochrome P450 78A9 (CYP79A9) were significantly up-regulated, whereas the expression of PISTILLATA (PI), TATA Box Associated Factor II 59 (TAFII59) and MYB Domain Protein 108 (MYB108) that were important for male organ development was down-regulated in response to 6-BA treatment, demonstrating that cytokinin could directly target the floral organ identity genes to regulate the flower sex. CONCLUSIONS: Our work demonstrated that cytokinin is a potential regulator in female flower development in S. sebiferum. The transcriptome analysis of the floral sex transition from androecious to monoecious in response to cytokinin treatment on the androecious S. sebiferum provided valuable information related to the mechanism of sex determination in the perennial woody plants.
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Citocininas/farmacologia , Flores/genética , Redes Reguladoras de Genes , Reguladores de Crescimento de Plantas/farmacologia , Sapium/genética , Transcriptoma , Compostos de Benzil/farmacologia , Flores/efeitos dos fármacos , Flores/crescimento & desenvolvimento , Perfilação da Expressão Gênica , Compostos de Fenilureia/farmacologia , Purinas/farmacologia , Sapium/efeitos dos fármacos , Sapium/crescimento & desenvolvimento , Tiadiazóis/farmacologiaRESUMO
Sapium sebiferum, an ornamental and bio-energetic plant, is propagated by seed. Its seed coat contains germination inhibitors and takes a long time to stratify for germination. In this study, we discovered that the S. sebiferum seed coat (especially the tegmen) and endospermic cap (ESC) contained high levels of proanthocyanidins (PAs). Seed coat and ESC removal induced seed germination, whereas exogenous application with seed coat extract (SCE) or PAs significantly inhibited this process, suggesting that PAs in the seed coat played a major role in regulating seed germination in S. sebiferum. We further investigated how SCE affected the expression of the seed-germination-related genes. The results showed that treatment with SCE upregulated the transcription level of the dormancy-related gene, gibberellins (GAs) suppressing genes, abscisic acid (ABA) biosynthesis and signalling genes. SCE decreased the transcript levels of ABA catabolic genes, GAs biosynthesis genes, reactive oxygen species genes and nitrates-signalling genes. Exogenous application of nordihydroguaiaretic acid, gibberellic acid, hydrogen peroxide and potassium nitrate recovered seed germination in seed-coat-extract supplemented medium. In this study, we highlighted the role of PAs, and their interactions with the other germination regulators, in the regulation of seed dormancy in S. sebiferum.
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Pecan is an economically important nut crop tree due to its unique texture and flavor properties. The pecan seed is rich of unsaturated fatty acid and protein. However, little is known about the molecular mechanisms of the biosynthesis of fatty acids in the developing seeds. In this study, transcriptome sequencing of the developing seeds was performed using Illumina sequencing technology. Pecan seed embryos at different developmental stages were collected and sequenced. The transcriptomes of pecan seeds at two key developing stages (PA, the initial stage and PS, the fast oil accumulation stage) were also compared. A total of 82,155 unigenes, with an average length of 1,198 bp from seven independent libraries were generated. After functional annotations, we detected approximately 55,854 CDS, among which, 2,807 were Transcription Factor (TF) coding unigenes. Further, there were 13,325 unigenes that showed a 2-fold or greater expression difference between the two groups of libraries (two developmental stages). After transcriptome analysis, we identified abundant unigenes that could be involved in fatty acid biosynthesis, degradation and some other aspects of seed development in pecan. This study presents a comprehensive dataset of transcriptomic changes during the seed development of pecan. It provides insights in understanding the molecular mechanisms responsible for fatty acid biosynthesis in the seed development. The identification of functional genes will also be useful for the molecular breeding work of pecan.
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Carya/crescimento & desenvolvimento , Perfilação da Expressão Gênica/métodos , Metabolismo dos Lipídeos , Proteínas de Plantas/genética , Carya/genética , Regulação da Expressão Gênica no Desenvolvimento , Regulação da Expressão Gênica de Plantas , Sequenciamento de Nucleotídeos em Larga Escala , Anotação de Sequência Molecular , Sementes/genética , Sementes/crescimento & desenvolvimento , Análise de Sequência de RNA/métodosRESUMO
Melatonin has emerged as a research highlight regarding its important role in regulating plant growth and the adaptation to the environmental stresses. In this study, we investigated how melatonin prevented the cadmium toxicity to wheat seedlings. The results demonstrated that cadmium induced the expression of melatonin biosynthesis-related genes and cause a significant increase of endogenous melatonin level. Melatonin treatment drastically alleviated the cadmium toxicity, resulting in increased plant height, biomass accumulation, and root growth. Cadmium and senescence treatment significantly increased the endogenous level of hydrogen peroxide, which was strictly counterbalanced by melatonin. Furthermore, melatonin treatment caused a significant increase of GSH (reduced glutathione) content and the GSH/GSSG (oxidized glutathione) ratio. The activities of two key antioxidant enzymes, ascorbate peroxidase (APX) and superoxide dismutase (SOD), but not catalase (CAT) and peroxidase (POD), were specifically improved by melatonin. Additionally, melatonin not only promoted the primary root growth, but also drastically enhanced the capacity of the seedling roots to degrade the exogenous hydrogen peroxide. These results suggested that melatonin played a key role in maintaining the hydrogen peroxide homeostasis, via regulation of the antioxidant systems. Conclusively, this study revealed a crucial protective role of melatonin in the regulation of cadmium resistance in wheat.
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Cádmio/toxicidade , Peróxido de Hidrogênio/metabolismo , Melatonina/farmacologia , Plântula/crescimento & desenvolvimento , Triticum/crescimento & desenvolvimentoRESUMO
Fluoride in trace quantity is beneficial for human beings, serving to strengthen the apatite matrix of skeletal tissues and teeth, whereas high intake causes adverse impacts. In the present study, the effect of fluoride-contaminated drinking water of livestock on the milk samples of different cattle, belonging to a fluoride-endemic area (Tharparkar, Pakistan), was studied. In milk samples of different cattle (cows, camels, sheep, and goats), free and bound fluoride forms and its total (free (F-) + bound (F-)) contents were measured by ion-selective electrode. The concentration of fluoride in drinking water of livestock was also analyzed, as found in the range of 11.8-33.5 mg/L. The concentration of total fluoride in the milk samples of sheep, goats, cows, and camels were observed in the range of 1.72-2.43, 1.40-2.03, 0.835-1.41, and 0.425-0.897 mg/L, respectively. The resulted data indicated that the concentration of fluoride was higher in the milk samples of smaller cattle (sheep and goat), as compared to cow and camel. The fluoride in milk samples of all cattle appeared dominantly in free form. The percentage values of bound fluoride in the milk samples of sheep, goats, and cows were found to be 6.76, 11.6, and 19.7% in total, respectively, while in camel milk, the percentage was below the detection limit. The estimated daily intake of fluoride contents on consuming different types of milk by children age ranged 1.0 to 3.0 years was evaluated. Graphical abstract á .
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Exposição Dietética/análise , Água Potável/química , Fluoretos/análise , Leite/química , Animais , Bovinos , Criança , Pré-Escolar , Feminino , Cabras , Humanos , Lactente , Gado , Paquistão , Medição de Risco , OvinosRESUMO
In this work, synthesis of novel symmetrical 4-(2-bromo-4-(5-bromo-1H-benzo[d] imidazol-2-yl) phenoxy) tetra substituted zinc phthalocyanine has been reported. The novel benzimidazole zinc phthlocynine compound (3) has been characterized by MALDI-TOF MS, FT-IR, UV-vis, and 1H NMR spectroscopy. This new compound 3 displayed excellent selectivity towards Bi3+ ion in the presence of other competitive ions including Ca2+, Cd2+, Co2+ Cu2+, Fe3+, Hg2+, Sn2+, Mg2+, Na+, Ni2+ and Pb2+ respectively. Upon addition of Bi3+ into the solution of compound 3 in DMSO, dramatic change was observed in the Q- and the B-bands in UV-visible spectra as a result of donor acceptor interactions. Reactive oxygen species (ROS) were also studied using 2,7-dichlorofluorescin diacetate (DCFH-DA) a fluorescent probe which is converted to highly fluorescent dichlorofluorescein (DCF) in the presence of ROS. This property of non-aggregating zinc phthalocyanine is promising as a photosensitizer in photodynamic therapy of cancer.
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Benzimidazóis/química , Benzimidazóis/síntese química , Bismuto/análise , Indóis/química , Indóis/síntese química , Compostos Organometálicos/química , Compostos Organometálicos/síntese química , Espécies Reativas de Oxigênio/química , Elétrons , Íons , Isoindóis , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Espectrofotometria Ultravioleta , Compostos de ZincoRESUMO
A novel microextraction procedure based on Schiff's base functionalized magnetic nanoparticles (SBMNPs) has been developed for Pb(+2) extraction. Compared to conventional microextraction systems, the main advantage of proposed procedure is that no volatile/flammable reagents have used and experimental time is also reduced. Schiff's base has been derived from salicylaldehyde and 3-aminopropyltriethoxysilane modified nanoparticles. Extraction of Pb(+2) was carried out in a capillary column containing analyte followed by the addition of SBMNPs and triton X-114 where ionic-liquid "1-Butyl-3-methylimidazolium hexafluorophosphate" was used as an extractant. After extraction; analyte concentration was determined with flame atomic absorption spectrometer through a self made micro-injection system. The SBMNPs were characterized by FTIR and TEM, respectively, demonstrating their distinct core-shell structures. Different experimental parameters were optimized through multivariate strategy. Detection limit, enhancement factor and relative standard deviation obtained with developed procedure were 0.193µgL(-1), 26.3 and 4.01%, respectively. Validity was checked through the recovery experiments and satisfactory results were obtained. In brief the synergistic combination of SBMNPs in column with ionic-liquid resulted in an efficient microextraction procedure for Pb(+2) in real samples.
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In this paper, we report a new liquid-liquid microextraction procedure called "nanoparticles decorated with a Schiff's base for the microextraction of Cd, Pb, Ni, and Co in environmental samples". The developed procedure was utilized for the extraction of Cd, Pb, Ni, and Co in environmental samples. The Schiff's base was formed by reacting salicylaldehyde with 3-aminopropyltriethoxysilane-functionalized iron oxide nanoparticles. Analyte extraction was conducted in a capillary column system loaded with modified nanoparticles and triton X-114 as dispersion medium. 1-Butyl-3-methylimidazolium hexafluorophosphate was employed as an extraction solvent. Acidified methanol in ultrasonic bath was used as desorption solvent, and elemental determination was carried out with flame atomic absorption spectrometer. Characterization of modified nanoparticles was performed with FTIR spectroscopy and transmission electron microscopy. Solution pH, nanoparticles amount, dispersant concentration, ionic liquid, and temperature were optimized for the extraction. Detection limits obtained for Cd, Pb, Ni, and Co were 0.183, 0.201, 0.241, and 0.192 µg L(-1), respectively, and enhancement factors were 79.1, 86.4, 95.7, and 82.0, respectively. The reproducibility of the developed procedure was in the range of 3.98-5.10%. Validation was checked by applying the developed procedure on certified reference water samples. The microextraction based on nanoparticles decorated with Schiff's base was successfully applied for the extraction of Cd, Pb, Ni, and Co in real environmental water samples.