The protective effects of quercetin on the physiological responses in malathion-exposed common carp, Cyprinus carpio.

This study aimed to evaluate the protective effects of quercetin on the biochemical parameters, immunity, and growth performance in malathion-exposed common carp, Cyprinus carpio. The methods six experimental groups, including the control group, fish exposed to concentrations of 1.04 and 2.08 mg/l malathion, fish supplemented with quercetin (200 mg/kg diet), and fish treated with quercetin + malathion for 21 days, were considered for the experiment. After the feeding period, in results the activities of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione S-transferase (GST) were significantly decreased in the hepatocyte, while malondialdehyde (MDA) content increased in response to malathion. Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities and glucose, cortisol, and urea levels significantly increased after exposure to malathion. Exposure of fish to malathion-induced decreases in protease, lysozyme, and alternative complement (ACH50) activities and total immunoglobulin (total Ig) in the mucosa. Changes in other parameters were different depending on malathion concentrations. The supplementation of fish with quercetin had no ameliorating effect on the malathion-related alternations of mucosal lysozyme and protease activities. However, quercetin ameliorated the depressing effects of malathion on biochemical and immunological parameters. Changes in the growth performance and hematological parameters indicated the toxic effect of malathion. In conclusion, quercetin could efficiently reduce the toxic effects of malathion on the biochemical, immune, and hematological parameters of the common carp.

Effect of Dietary Lactobacillus casei on Physiometabolic Responses and Liver Histopathology in Common Carp (Cyprinus carpio) After Exposure to Iron Oxide Nanoparticles.

A 60-day feeding trial was performed to assess the dietary effect of Lactobacillus casei as a probiotic supplement on some serum biochemical parameters and liver histopathology in common carp fry after exposure to iron oxide nanoparticles (IoNPs). Six treatments were prepared as follows: control (no IoNP exposure and no dietary probiotic), P6: 106 CFU/g probiotic diet, P7: 107 CFU/g probiotic diet, NPs: 0.15 mg/l IoNPs, NPs + P6: 0.15 mg/l IoNPs with 106 CFU/g probiotic diet, and NPs + P7: 0.15 mg/l IoNPs with 107 CFU/gprobiotic diet. Based on the results, serum aspartate aminotransferase and alanine aminotransferase levels were significantly increased in 0.15 mg/l IoNPs, P7, and NPs + P6 treatments compared to the control group. In addition, the examination of antioxidant enzymes showed a significant increase in the levels of cortisol and glutathione S-transferase as well as malondialdehyde level. IoNPs also caused significant histopathological changes in the fish liver during the experiment such as hyperemia in sinusoidal spaces, hepatocytes vacuolation and necrosis, pyknosis, and disruption of hepatic lobules and atrophy. Results revealed the protective effects of dietary L. casei to mitigate the adverse impacts of IoNPs on the physiological processes of common carp.