RESUMO
To characterize the complete genome sequence of coxsackievirus B1 (CVB1) MSH/KM9/2009 strain isolated from Yunnan, China,2009. Eight overlapping clones covering the whole viral genome (excluding the poly-A tail) were obtained by RT-PCR and sequenced, and their nucleotide and amino acid sequences were compared with other known CVB1 strains. The genome of the CVB1 MSH/KM9/2009 strain had 7384 nucleotides in length, and contained a 741nt non-translated region (NTR) at the 5' end and a 94nt NTR at the 3' end. The entire open reading frame contained 6 549 nt, encoding a 2 183-aa polyprotein. In the coding region, there was no nucleotide deletion or insertion, but some changes of amino acid were unique. The complete genome sequence alignments showed that the CVB1 isolate MSH/KM9/2009 strain shared the highest nucleotide (80.9%, 81.6%, 80.5% and 80%) and amino acid (95.6%, 95.8%, 96.2% and 95.6%) identities to the CVB1 M16560, pmMC, Tucson B1 and CVB1Nm strain, respectively. Phylogenetic tree analysis showed that the MSH/KM9/2009, CVB1 M16560, pmMC, Tucson B1 and CVB1Nm strain clustered into same group. The newly isolated CVB1 strain MSH/KM9/2009 from Yunnan Province belonged to genotype CVB1.
Assuntos
Infecções por Coxsackievirus/virologia , Enterovirus/genética , Enterovirus/isolamento & purificação , Genoma Viral , Sequência de Bases , Pré-Escolar , China , Enterovirus/classificação , Feminino , Genótipo , Humanos , Lactente , Masculino , Fases de Leitura Aberta , Filogenia , Proteínas Virais/genéticaRESUMO
OBJECTIVE: To analyze the genetic characterization of the complete genome from a human coxsackievirus B3 strain A103/KM/09 isolated in Yunnan province, 2009. METHODS: By using RT-PCR, all the eight fragments which containing about 1000 nucleotides and covering full viral genome, were sequenced. By using Mega 5.05,Geneious, RDP 3 and SimPlot 3.5.1 software, sequences were aligned with other enterovirus reference sequences. Phylogenetic and recombination analysis were also carried out. RESULTS: The A103/KM/09 isolate genome showed 7389 nucleotides in length , encoding for 2185 amino acids. In the complete genome, the homology of nucleotide and amino acid among the seven coxsackievirus B3 isolates were 81.0%-88.0% and 95.7%-98.0%, respectively. There appeared 81.0% and 95.7% homology when compared with that of Nancy prototype strain. Results from the Phylogenetic analysis showed that the coxsackievirus B3 formed five distinct clades, I-V. Nucleotide divergence rates between clades were 16.2%-24.3% . The A103/KM/09 strain belonged to clade V. Clade V was further divided into four sub-clades,A-D. The nucleotide divergence between sub-clades was 4.3%-11.4%. Putative recombinant event for A103/ KM/09 was detected. CONCLUSION: All coxsackievirus B3 isolates could be divided into five clades, with A103/KM/09 strain belonged to Clade V-D. Evolution of coxsackievirus B3 had occurred in China.
Assuntos
Encefalite Viral/virologia , Enterovirus Humano B/genética , Enterovirus Humano B/isolamento & purificação , Infecções por Enterovirus/virologia , Sequência de Bases , Pré-Escolar , China/epidemiologia , Encefalite Viral/epidemiologia , Infecções por Enterovirus/epidemiologia , Genoma Viral , Humanos , Masculino , Filogenia , Proteínas Virais/genéticaRESUMO
The complete nucleotide sequence of two human enterovirus 71 strains (KMM09 and KM186-09) isolated in Yunnan,China, were determined by RT-PCR and sequencing. As with other human enteroviruses, the genomes were 7 409 nucleotides (nts) in length and encoded 2 193 aa. Phylogenetic analysis based on VP1 regions revealed that the two isolates belonged to subgenotype C4a. In structural genomic regions, subgenotype C4 was most homologous to other strains of C genotype when compared to other genotypes. In non-structural genomic regions, subgenotype C4 was more homologous to CA16/G10 and other strains of B genotype than to other strains of C genotype. RDP3 and Blast analysis displayed evidence of recombination in non-structural genomic regions between subgenotype B3 and C4, C4 and CA16/G10. The full-length genome of the human enterovirus 71 strains provided an overview of the diversity of genetic characteristics of a circulatinghuman enterovirus 71.
Assuntos
Enterovirus Humano A/genética , Enterovirus Humano A/isolamento & purificação , Genoma Viral , Animais , Sequência de Bases , China , Chlorocebus aethiops , Enterovirus Humano A/classificação , Fezes/virologia , Genótipo , Humanos , Dados de Sequência Molecular , Filogenia , Células VeroRESUMO
Based upon the World Health Organization (WHO)'s perspective, the post-eradication era is coming with a priority of global certifying the wild poliovirus eradication, by which then the Oral Poliomyelitis Attenuated Live Vaccine (OPV) will be ceased and Inactivated Poliovirus Vaccine (IPV) will be synchronized used, in particular the affordable Sabin-IPV (sIPV) is recommended to be used continuously in developing countries till the final eradication of poliomyelitis. However, no any sIPV has successfully been developed in the world. In this paper, the sIPV immunization strategy, current development status and its usage in industrialized countries are preliminarily analyzed. Feasibility analysis of potential sIPV usage in China, including immunization strategy, schedule, production technology and supply capacity is analysed as well. sIPV development in China should be accelerated in order to prepare production technology to the post-eradication era.
