RESUMO
Under conditions of COVID-19 pandemic, considerable amounts of SARS-CoV-2 contained in household, municipal, and medical wastewaters inevitably reach natural water bodies. Possible preservation of virus infectivity in liquid environment is of a paramount epidemiological importance. Experiments demonstrated that SARS-CoV-2 is resistant to multiple freezing/thawing cycles and retains its infectivity in tap and river water for up to 2 days at 20°C and 7 days at 4°C. In natural milk, its viability is preserved in a refrigerator for 6 days. The exposure of aquarium fish to the virus-containing water fails to cause any infection.
Assuntos
COVID-19 , Animais , Humanos , Pandemias , SARS-CoV-2 , Águas Residuárias , ÁguaRESUMO
Different methods for producing bulk quantities of concentrated and purified SARS-CoV-2 for the use as antigens and for the research into COVID-19 biology were tested.
Assuntos
Teste para COVID-19/métodos , COVID-19/diagnóstico , SARS-CoV-2/imunologia , Carga Viral/imunologia , Vírion/imunologia , Animais , Anticorpos Neutralizantes/isolamento & purificação , Anticorpos Neutralizantes/metabolismo , Anticorpos Antivirais/isolamento & purificação , Anticorpos Antivirais/metabolismo , COVID-19/virologia , Linhagem Celular , Chlorocebus aethiops , Convalescença , Células Epiteliais/virologia , Humanos , Soros Imunes/química , SARS-CoV-2/genética , SARS-CoV-2/crescimento & desenvolvimento , Suínos , Células Vero , Carga Viral/genética , Proteínas Virais/imunologia , Proteínas Virais/isolamento & purificação , Proteínas Virais/metabolismo , Vírion/genética , Vírion/crescimento & desenvolvimento , Replicação ViralRESUMO
The effects of recombinant analog of natural Ebola virus protein vp24 in configurations virulent (vp24-ad) and avirulent (vp24-w) for guinea pigs on interferonogenesis were studied in vivo and in vitro. Amino acid differences were determined by His186 substitution in avirulent (nonlethal) configuration for Tyr in the virulent (lethal) one. Recombinant analogs vp24-w and vp24-ad inhibited interferonogenesis in vivo and in vitro. Inhibition by the two protein configurations was virtually the same.