Effects of Camellia oleifera seed shell polyphenols and 1,3,6-tri-O-galloylglucose on androgenic alopecia via inhibiting 5a-reductase and regulating Wnt/ß-catenin pathway.

Androgenetic alopecia (AGA) is the leading cause of hair loss in adults. Its pathogenesis remains unclear, but studies have shown that the androgen-mediated 5α-reductase-AR receptor pathway and the Wnt/ß-catenin signaling pathway play significant roles. Camellia oleifera is an oil plant, and its fruits have been documented in folklore as having a hair cleansing effect and preventing hair loss. In this study, we used UPLC-Q-TOF-MS/MS to identify the structure of the substances contained in the polyphenols of Camellia oleifera seed shell. These polyphenols are mainly used for shampooing and anti-hair loss purposes. Next, we used molecular docking technology to dock 41 polyphenols and steroidal 5 alpha reductase 2 (SRD5A2). We found that the docking scores and docking sites of 1,3,6-tri-O-galloylglucose (TGG) and finasteride were similar. We constructed a mouse model of DHT-induced AGA to evaluate the effects of Camellia oleifera seed shell polyphenols (CSSP) and TGG in vivo. Treatment with CSSP and TGG alleviated alopecia symptoms and reduced DHT levels. Additionally, CSSP and TGG were able to reduce androgen levels by inhibiting the SRD5A2-AR receptor signaling pathway. Furthermore, by regulating the secretion of growth factors and activating the Wnt/ß-catenin signaling pathway, CSSP and TGG were able to extend the duration of hair growth. In conclusion, our study showed that CSSP and TGG can improve AGA in C57BL/6 J mice and reduce the effect of androgen on hair follicle through the two signaling pathways mentioned above. This provides new insights into the material basis and mechanism of the treatment of AGA by CSSP.

Molecular mechanism for the influence of yam starch multiscale structure on the sensory texture of cooked yam.

Yam is a dual-purpose crop as both medicine and food. However, the mechanism controlling the eating quality of yam remains to be elucidated. This study explored the influence of starch multiscale structure on the texture of yam. The results indicated that FS and RC yam have higher hardness and chewiness, while BZ, XM, and PL yam possess waxiness, Fineness, and Stickiness. Statistically, high amylose (AM) can increase hardness, chewiness, and compactness; and average molecular size (Rh) is positively correlated with stickiness, fineness, and waxiness. Specifically, medium- and long-chain amylose (1000 < X ≤ 10,000) and amylopectin (24 < X ≤ 100), particularly medium-chain amylose (1000 < X ≤ 5000) and long-chain amylopectin (24 < X ≤ 36), primarily affect sensory and rheological stickiness. The long chains of amylose form a straight chain interspersed in the crystalline and amorphous regions to support the entire lamellar structure. Higher proportion of amylose long chains, promoting the starch's structural rigidity, which in turn enhanced its hardness-related attributes. Moreover, a higher ratio of long chains within amylopectin results in tightly intertwined adjacent outer chains, forming double helix crystalline zones. This consequently augmenting the texture quality linked to stickiness-related attributes.

Addressing unforeseen public health risks via the use of sustainable system and process management.

During the coronavirus disease 2019 (COVID-19) pandemic, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which was designated by the World Health Organization in January 2020 as a newly emerging coronavirus in 2019, and its variants have placed unbearable strain on the healthcare systems of various countries, with serious implications for sustainable development worldwide. Researchers have proposed several solutions, such as the use of digital technologies to improve prevention systems. However, the challenges of epidemic prevention and control failures have not been addressed fundamentally, as the key causes of epidemic failures (i.e., outbreaks) and strategies for process management have been neglected. The purpose of the current study is to address these issues by exploring the causes of epidemic prevention and control failure and targeting improvement strategies that combine system structure of epidemic prevention and process management. Specifically, following an exploration of the main reasons for COVID-19 prevention and control failures through a case study of two tertiary hospitals, this paper outlines a targeted prevention and control system based on triangular validation and a loosely coupled process management framework and verifies the expected results using simulation methods together with statistical data on the spread of SARS-CoV-2 in Wuhan, China. The findings not only advance the development of epidemic risk prevention and control theory, especially the complementary nature of IT applications and process management in the field of epidemic risk prevention and control, but also provide guidance on the innovation and implementation of epidemic prevention and control systems and process management and recommendations for countries to promote sustainable development from a health-focused perspective.

Does corporate carbon performance converge in the global market? Evidence from a distribution dynamic approach.

Converging corporate carbon performance (CCP) to a higher level is necessary to achieve the global goal of controlling temperature rise. However, it remains uncertain whether all international firms endeavour to improve CCP. Using a panel of 19,913 public companies from 76 countries during the 2010-2019 period and two visual tools of the distribution dynamics approach, we conduct a nascent analysis of transitional dynamics and the long-run evolution of CCP. We find that regardless of investigated period (before and after Paris Agreement) and regional location, most firms converge towards the highest CCP of 10, thereby improving carbon performance over time. After Paris Agreement, the convergence to the top CCP is more significant, whereas more companies cluster around the mediocre CCP (a value of 6.7), thus evidencing an increased heterogeneity in convergence paths. Firms from East Asia & Pacific and the North American regions drive such heightened heterogeneity. Specifically, enterprises from East Asia & Pacific show the least convergence towards the highest CCP, probably because more manufacturing firms in the region primarily rely on fossil fuels and face loose environmental regulations. Therefore, further improving CCP may require substantial investments in equipment upgrades and would result in significantly higher costs. For North America, the results can be associated with Donald Trump's policy towards climate change and bid to withdraw from the Paris Agreement, reflecting firms taking a Republican stand, most likely diverging to mediocre CCP and experiencing a decline in future carbon management. The observed convergence towards the highest CCP is nearly twice as significant among firms from OECD than non-OECD countries, which aligns with global enterprises outsourcing emissions to developing countries. The study reveals the pattern of strong convergence to the highest CCP in the global firms as evidence of collective efforts in the transition to net zero. However, divergence and increased heterogeneity may occur in some regions due to politics, industrial structure and regulations.

Institutional development and the government response to COVID-19 in China.

As COVID-19 is pervasive across the globe, governments in different countries face the dilemma of restricting the transmission risk of the virus by social distancing while yet maintaining economic activity. Inadequate social distancing policies lead to more infection cases and deaths, while over stringent social distancing policies have significant economic cost implications. This study investigates the role of local government institutions in striking the balance between saving lives and economic recovery. We based our study on a sample of 28 provincial governments in China during the early outbreak of 2020 when the emergency responses of local governments were synchronous. The findings show that local governments in those provinces with lower degrees of marketization, which were accustomed to directly intervene in the social system, mandatorily quarantined many more close contacts for each confirmed case than those in the more market-oriented provinces whose social distancing policies took economic considerations into account. The 'overdone' (over stringent) social distancing policies in the more state-oriented provinces led to lower human mobility and economic growth. This study highlights the importance of taking economic considerations into account when adopting policies and strategies to combat the spread of COVID-19 and how different institution management cultures lead to different outcomes.

The influence of phytochemicals on cell heterogeneity in chronic inflammation-associated diseases: the prospects of single cell sequencing.

Chronic inflammation-associated diseases include, but is not limited to cardiovascular disease, cancer, obesity, diabetes, etc. Cell heterogeneity is a prerequisite for understanding the physiological and pathological development of cell metabolism, and its response to external stimuli. Recently, dietary habits based on phytochemicals became increasingly recognized to play a pivotal role in chronic inflammation. Phytochemicals can relieve chronic inflammation by regulating inflammatory cell differentiation and immune cell response, but the influence of phytochemicals on cell heterogeneity from in vitro and ex vivo studies cannot simulate the complexity of cell differentiation in vivo due to the differences in cell lines and extracellular environment. Therefore, there is no consensus on the regulation mechanism of phytochemicals on chronic diseases based on cell heterogeneity. The purpose of this review is to summarize cell heterogeneity in common chronic inflammation-associated diseases and trace the effects of phytochemicals on cell differentiation in chronic diseases development. More importantly, by discussing the problems and challenges which hinder the study of cell heterogeneity in recent nutritional assessment experiments, we propose new prospects based on the drawbacks of existing research to optimize the research on the regulation mechanism of phytochemicals on chronic diseases. The need to explore precise measurements of cell heterogeneity is a key pillar in understanding the influence of phytochemicals on certain diseases. In the future, deeper understanding of cell-to-cell variation and the impact of food components and their metabolites on cell function by single-cell genomics and epigenomics with the focus on individual differences will open new avenues for the next generation of health care.

COVID-19 and Regional Income Inequality in China.

This study investigates the impact of COVID-19 and social distancing policies on regional income inequality. We base our study on a sample of 295 prefecture (and above) cities in 31 provinces in China. A distribution dynamics approach is employed to reveal the trend and movement of disposable income per capita in each city before the COVID-19 pandemic, during the COVID-19 pandemic, and in the period when the COVID-19 was under the control. The findings reveal significant negative economic consequences of the COVID-19 in the first quarter of 2020 and show that most cities will converge to a level of disposable income which is much less than the Pre-COVID level if the COVID pandemic persists. Regional income inequality has intensified in the cities that have a longer duration of stringent social distancing policies during the COVID-19 pandemic and disappeared in the cities with policies of short duration. Disposable income per capita for urban residents recovered quickly when the transmission of coronavirus was effectively contained; and yet the impact of the pandemic on rural residents remains unresolved, if not intensified. This study demonstrates a significant divergence of the trend of disposable income across cities with different durations of social distancing policies and between urban and rural residents. It also highlights the importance of stringent social distancing policies in containing the spread of virus in a short time and calls for special policy attention for rural regions in the recovery from the COVID-19.

[Determination of 3-monochloropropane-1,2-diol in grease food by solid phase extraction-derivatization-gas chromatography-mass spectrometry].

OBJECTIVE: To establish the method of determination of 3-monochloropropane-1,2-diol( 3-MCPD) in grease food by gas chromatography-mass spectrometry( GC-MS). METHODS: 3-MCPD in grease food represented by bean paste was extracted by ultrasound,purified by alkaline earth solid phase extraction column,derivatived using phenylboronic acid( PBA) and detected by GC-MS. RESULTS: The linearity of 3-MCPD ranged from 1-100 ng/mL,with correlation coefficient at 0. 9993.The limits of quantitation( LOQ) in soy sauce,bean paste,pepper oil were 0. 6,0. 5 and7. 0 µg/kg and limits of detection( LOD) were 1. 9,1. 6 and 18. 8 µg/kg,respectively.Average recovery rate and relative standard deviation was 78. 3%-106. 7% and 1. 9%-11. 6%( n = 6), when 3-MCPD was added in grease food at 2. 5-1000 µg/kg. CONCLUSION: The method has good purification effect and the detection sensitivity and accuracy,and can be used for the determination of 3-MCPD in grease food.

Mutagenicity and safety evaluation of the water extract of Camellia oleifera Abel.

UNLABELLED: The purpose of this study was to evaluate the mutagenicity and safety of water extract of the fruit hull of Camellia oleifera Abel (WECO), which was prepared using hot-reflux method. The oral maximum tolerated dose (MTD) of WECO was above 20 g/kg body weight both in rats and in mice, which can be regarded as virtually nontoxic. No mutagenicity was found in Ames test, mouse bone marrow cell micronucleus test and mouse sperm abnormality test. In the subacute study, the SD rats were administered orally at 0.5, 1, or 2 g/kg/BW for 30 d. There were no treatment-related toxic effects from WECO. No significant differences were found in parameters of body weight, hematology value, clinical chemistry value, and organ/body weight ratio. The level of no observed adverse effect level (NOAEL) for WECO was 2 g/kg/BW for subacute toxicity study. PRACTICAL APPLICATION: With the gradual increase in tea oil production, it was in urgent need of dealing with Camellia fruit hull, which was always discarded because of low economic benefits. Camellia fruit hull has been shown to have significant antioxidant effects including DPPH radical-scavenging ability and ferric-reducing antioxidant power (Zhang and others 2010). Toxicological evaluation of WECO provided a safety assurance of WECO for developing dietary supplements and functional foods.

Fatty acid synthase inhibitors separated from oiltea camellia by high-speed counter-current chromatography.

UNLABELLED: The inhibitory effects of oiltea camellia extracts (OCEs) and its active components on fatty acid synthase (FAS) were investigated. OCE potently inhibited the activity of FAS with an half-maximal inhibitory concentration (IC50) value of 2.30 µg/mL. The inhibition kinetic results showed that OCE and acetyl-CoA competitively inhibited FAS but these compounds exhibited mixed inhibition against malonyl-CoA and NADPH. Further study uncovered that the active components of OCE, ellagic acid (EA) and 3-O-methylellagic acid 4'-O-ß-D-glucopyranoside (MEAG), which were isolated and purified by high-speed counter-current chromatography (HSCCC) using a 2-phase solvent system of chloroform-ethanol-water-acetic acid (4:3:2:0.01, v/v/v/v), inhibited FAS with IC50 of 2.50 and 37.73 µg/mL, respectively. Their inhibition kinetics were different from that of OCE. Both of them exhibited uncompetitive inhibition for nicotinamide adenine dinucleotide phosphate (NADPH) and decreased the FAS activity through inactivation of acetyl/malonyl transferase on FAS. These results suggest that OCE could be a valuable resource for bioactive substances. PRACTICAL APPLICATION: With the gradual increase in tea oil production, it was in urgent need of dealing with Camellia fruit hull, which was always discarded because of low economic benefits. Camellia fruit hull has been shown to have significant antioxidant effects including 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging ability and ferric-reducing antioxidant power. This study found that the ethanolic extract of Camellia fruit hull at low concentration efficiently inhibited FAS activity, which is a potential therapy target of both obesity and cancer. These results suggest that OCE could be a valuable resource for bioactive substances.

Molecular structure of selected tuber and root starches and effect of amylopectin structure on their physical properties.

The objectives of this study were to characterize starches isolated from potato, canna, fern, and kudzu, grown in Hangzhou, China, for potential food and nonfood applications and to gain understandings of the structures and properties of tuber and root starches. Potato and canna starches with B-type X-ray patterns had larger proportions of amylopectin (AP) long branch chains (DP >or=37) than did fern (C-type) and kudzu (C(A)-type) starches. The analysis of Naegeli dextrins suggested that fern and kudzu starches had more branch points, alpha-(1,6)-D-glycosidic linkages, located within the double-helical crystalline lamella than did the B-type starches. Dispersed molecular densities of the C- and C(A)-type APs (11.6-13.5 g/mol/nm(3)) were significantly larger than those of the B-type APs (1.4-6.1 g/mol/nm(3)) in dilute solutions. The larger proportion of the long AP branch chains in the B-type starch granules resulted in greater gelatinization enthalpy changes (Delta H). Retrograded kudzu starch, which had the shortest average chain length (DP 25.1), melted at a lower temperature (37.9 degrees C) than the others. Higher peak viscosities (550-749 RVU at 8%, dsb) of potato starches were attributed to the greater concentrations of phosphate monoesters, longer branch chains, and larger granule sizes compared with other tuber and root starches.