Elevated temperature magnifies the acute and chronic toxicity of clothianidin to Eisenia fetida.

Pesticide residue and thermal stress resulting from global climate change are parallel stressors for soil fauna. However, it remains ambiguous how elevated temperatures and pesticides can interact to threaten soil fauna. In the study, the acute and chronic clothianidin (CTD) toxicity to earthworms (Eisenia fetida) at different temperatures, and the effect of increasing temperature on antioxidant defense mechanisms in response to CTD were investigated. The acute toxicity of CTD was exacerbated by increased temperature in both filter paper contact tests (a decrease in the 48-h median lethal concentration (LC50) from 0.077 µg/cm2 at 20 °C to 0.009 µg/cm2 at 30 °C) and natural soil tests (a decrease in the 48-h LC50 from 0.774 mg/kg at 20 °C to 0.199 mg/kg at 30 °C). Exposure to CTD or high temperature (30 °C) triggered reactive oxygen species (ROS) overgeneration and increased antioxidant enzyme activities in earthworms; and the effect was particularly pronounced after exposure to both higher temperatures and CTD. At 20 and 25 °C, there was no significant change in the growth and reproduction of E. fetida after 56-d exposure to CTD-contaminated soil. However, the combined effect of CTD and high temperature (30 °C) significantly reduced the weight change rate, cocoon number, hatching rate, and number of juveniles on day 56. These results indicated that elevated temperature could aggravate acute and chronic CTD toxicity to earthworms. The findings emphasize that evaluating changes in pesticide toxicity under global warming is worth further investigation.

Higher temperature and daily fluctuations aggravate clothianidin toxicity towards Limnodrilus hoffmeisteri.

In nature, aquatic organisms may suffer from chemical pollution, together with thermal stress resulted from global warming. However, limited information is available on the combined effects of pesticide with climate change on aquatic organisms. In this study, the acute toxicity of clothianidin to Limnodrilus hoffmeisteri as well as its effect on the induction of oxidative stress under both constant temperature and daily temperature fluctuation (DTF) regimes was investigated. Results showed that clothianidin exhibited the minimal toxicity to L. hoffmeisteri at 25 °C, which was magnified by both increased or decreased temperatures and 10 °C DTF. At different temperatures (15 °C, 25 °C and 35 °C), clothianidin exposure led to the elevated reactive oxygen species (ROS) levels and activated the antioxidant enzymes to resist against the oxidative stress. However, the antioxidant response induced by clothianidin was overwhelmed at high temperature as evidenced by decreased glutathione (GSH) content. Significant elevation of catalase (CAT) and peroxidase (POD) activities but depletion of GSH was also observed in worms treated with clothianidin under DTF after 24 h. The results indicated that high temperature and DTF could aggravate the clothianidin-induced oxidative stress. Moreover, the critical thermal maximum (CTmax) of the worms decreased with the increasing clothianidin concentrations, suggesting that exposure to clothianidin could reduce the heat tolerance of L. hoffmeisteri. Our work highlights the crucial importance to integrate temperature changes into risk assessment of pesticides under global warming.

Absorption, metabolism and distribution of carbosulfan in maize plants (Zea mays L.).

BACKGROUND: The insecticide carbosulfan is usually applied as a soil treatment or seed-coating agent, and so may be absorbed by crops and pose dietary risks. Understanding the uptake, metabolism and translocation of carbosulfan in crops is conducive to its safe application. In this study, we investigated the distribution of carbosulfan and its toxic metabolites in maize plants at both the tissue and subcellular levels, and explored the uptake and translocation mechanism of carbosulfan. RESULTS: Carbosulfan was mainly taken up by maize roots via the apoplast pathway, was preferentially distributed in cell walls (51.2%-57.0%) and most (85.0%) accumulated in roots with only weak upward translocation. Carbofuran, the main metabolite of carbosulfan in maize plants, was primarily stored in roots. However, carbofuran could be upwardly translocated to shoots and leaves because of its greater distribution in root-soluble components (24.4%-28.5%) compared with carbosulfan (9.7%-14.5%). This resulted from its greater solubility compared with its parent compound. The metabolite 3-hydroxycarbofuran was found in shoots and leaves. CONCLUSION: Carbosulfan could be passively absorbed by maize roots, mainly via the apoplastic pathway, and transformed into carbofuran and 3-hydroxycarbofuran. Although carbosulfan mostly accumulated in roots, its toxic metabolites carbofuran and 3-hydroxycarbofuran could be detected in shoots and leaves. This implies that there is a risk in the use of carbosulfan as a soil treatment or seed coating. © 2023 Society of Chemical Industry.

Triazole resistance in Aspergillus fumigatus exposed to new chiral fungicide mefentrifluconazole.

BACKGROUND: Triazole resistance in the human fungal pathogen Aspergillus fumigatus has been a growing challenge in clinic treatment with triazole drugs such as itraconazole. The fast evolvement of triazole resistance in A. fumigatus in the ecosystem has drawn great attention, and there has been a possible link between the application of triazole fungicides in agriculture and triazole resistance in A. fumigatus. The change in susceptibility of A. fumigatus exposed to the new chiral triazole fungicide mefentrifluconazole was investigated in this study. RESULTS: The results indicated that triazole resistance in A. fumigatus was acquired with exposure to mefentrifluconazole at a level of greater than or equal to 2 mg L-1 in liquid medium and soil (not at 0.4 nor 1 mg L-1 ). Interestingly, stereoselectivity was found in the acquisition of triazole resistance in A. fumigatus when exposed to mefentrifluconazole. R-mefentrifluconazole, which is very active on plant pathogens, exhibited stronger possibility in the development of the resistance in A. fumigatus than its antipode. Overexpression of cyp51A, AtrF, AfuMDR1 and AfuMDR4 were associated with the acquired resistance in A. fumigatus with hereditary stability. CONCLUSION: The results suggest that triazole resistance in A. fumigatus could be resulted from the selection of mefentrifluconazole at concentrations larger than 2 mg L-1 . Mefentrifluconazole should be applied within the dosage recommended by good agricultural practice to avoid the resistance in A. fumigatus in soil. This also may be applicable to other triazole fungicides. © 2022 Society of Chemical Industry.

Herbicidal activity of atrazine to barnyard grass depends upon soil characteristics.

BACKGROUND: The efficacy of a herbicide as soil treatment agent may be largely affected by soil characteristics. Understanding the relationship between herbicide efficacy and soil characteristics can provide decision basis for herbicide application according to local conditions. This study was aimed towards exploring the effect of soil characteristics on herbicidal activity of atrazine as a model herbicide to barnyard grass and thus to find an indicator for the herbicidal activity assessment of a herbicide against weeds. RESULTS: The herbicidal activity of atrazine to barnyard grass varied greatly among the tested soils with the medium inhibition concentration (IC50 ), based on the amended concentration, ranging from 1.07 to 10.91 mg kg-1 . Uptake of atrazine by barnyard grass was negatively correlated with its adsorption onto soils, whereas it was positively related to the concentration of the herbicide in in situ pore water (CIPW ). Comparable IC50 values ranging from 1.14 to 1.38 were obtained from CIPW in the tested soils with much smaller variation coefficient compared to those based on the traditional concentration (Csoil ) of this herbicide in soils determined by extraction with organic solvents. CONCLUSION: The concentration of atrazine in in situ pore water could be reliable to evaluate its bioavailability and herbicidal activity to barnyard grass. CIPW of a herbicide in soil could be an indicator for guiding the practical application rate. © 2022 Society of Chemical Industry.

Uptake, translocation, and metabolism of thiamethoxam in soil by leek plants.

Thiamethoxam (TMX) is commonly applied on leek plants by root irrigation. It might be taken up by leek plants and thus has lasting dietary risk. In this study, the uptake, translocation, and metabolism of TMX in leek plants were investigated. The results obtained from both the hydroponic and soil experiments indicated that TMX could be easily translocated upward and accumulated in leek shoots after being absorbed by roots. The total absorbed TMX amount (Mtotal) in leek plants from the tested soils varied greatly with its adsorption governed by soil characteristics. Interestingly, Mtotal was closely correlated with the concentration of TMX in in situ pore water, indicating that TMX in in situ pore water could be a useful approach to predict uptake of this chemical by leek plants from various soils. Profoundly, clothianidin (CLO) was detected with concentration of 0.07-1.54 mg/kg in roots and 0.27-4.12 mg/kg in shoots at 14 d, respectively, suggesting that TMX is easily converted into CLO in leek plants. The results showed that TMX used in soil is easily absorbed by leek and accumulated in edible parts accompanying with formation of CLO.

Sugar enhances waterlogging-induced adventitious root formation in cucumber by promoting auxin transport and signalling.

Waterlogging is a severe environmental stress that causes severe crop productivity losses. Cucumber (Cucumis sativus L.) survives waterlogging by producing adventitious roots (ARs) that enhance gas exchange. Little is known about the role of light and sugars in the waterlogging-induced production of ARs. The role of these factors in AR production was therefore studied in cucumber seedlings grown in the absence or presence of waterlogging and different light conditions. The effect of photosynthesis was studied by removing the shoots of the seedlings and replacing them with exogenous applications of sucrose or stachyose. Shoot removal inhibited AR emergence and elongation. However, the exogenous application of sugars fully restored AR emergence and partially restored root elongation. The exogenous application of a synthetic auxin restored AR emergence but not AR elongation. Transcriptome profiling analysis was used to determine the effects of light on gene expression in the hypocotyls under these conditions. The levels of transcripts encoding proteins involved in auxin transport and signalling were higher in the light and following the exogenous application of sucrose and stachyose. These results show that the waterlogging-induced emergence of ARs is regulated by the interaction between sugars and auxin, whereas AR elongation depends only on sugars alone.