Oral cancer diagnosis based on gated recurrent unit networks optimized by an improved version of Northern Goshawk optimization algorithm.

Oral cancer early diagnosis is a critical task in the field of medical science, and one of the most necessary things is to develop sound and effective strategies for early detection. The current research investigates a new strategy to diagnose an oral cancer based upon combination of effective learning and medical imaging. The current research investigates a new strategy to diagnose an oral cancer using Gated Recurrent Unit (GRU) networks optimized by an improved model of the NGO (Northern Goshawk Optimization) algorithm. The proposed approach has several advantages over existing methods, including its ability to analyze large and complex datasets, its high accuracy, as well as its capacity to detect oral cancer at the very beginning stage. The improved NGO algorithm is utilized to improve the GRU network that helps to improve the performance of the network and increase the accuracy of the diagnosis. The paper describes the proposed approach and evaluates its performance using a dataset of oral cancer patients. The findings of the study demonstrate the efficiency of the suggested approach in accurately diagnosing oral cancer.

Circ_0004771 regulates malignant biological behaviors and has clinical significance in oral squamous cell carcinoma.

BACKGROUND: The incidence of oral squamous cell carcinoma (OSCC) is increasing, and more effective treatment protocols must rapidly be developed to prevent the death of patients and ensure favorable outcomes. CircRNAs are a unique class of noncoding ribonucleic acid (RNA) molecules unaffected by RNA exonucleases. CircRNAs have more stable expression than linear RNAs and are not readily degraded; therefore, they are the newest focus of RNA research. Here, we analyze the mechanism of hsa_circ_0004771 (circ_0004771) in OSCC to provide a clinical reference. METHODS: Circ_0004771 expression was measured in peripheral blood, cancerous tissues and adjacent tissues of OSCC patients. Patients were followed up for 3 years. The diagnostic value of circ_0004771 for OSCC occurrence, prognosis, recurrence and survival was analyzed with receiver operating characteristic (ROC) curves. OSCC cells were lentivirally transduced with a circ_0004771-silencing or an empty vector to evaluate alterations in cell growth, invasion, and apoptosis. Apoptosis-related and epithelial-mesenchymal transition (EMT)-related protein expression was quantified. BALB/c nude mice were used for tumorigenesis experiments to evaluate tumor growth in vivo after silencing circ_0004771. RESULTS: Circ_0004771 expression was higher in peripheral blood and cancerous tissue of OSCC patients than in control peripheral blood and paracancerous tissue, respectively, exhibiting excellent predictive value for OSCC occurrence, prognosis, recurrence and survival. Silencing circ_0004771 decreased the growth, invasiveness, and EMT capacity and increased the apoptosis of OCC cells. In mice implanted with OSCC cells transduced with the circ_0004771-silencing lentiviral vector, the tumor growth capacity was obviously decreased. CONCLUSION: Silencing circ_0004771 inhibits the malignant growth of OSCC.

The role of long non-coding RNA (lncRNA) nuclear paraspeckle assembly transcript 1 (NEAT1) in chronic periodontitis progression.

Long non-coding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) is a novel pro-inflammatory factor in severe human diseases. Since inflammatory plays important roles in periodontitis progression, we aimed to explore the role of NEAT1 in chronic periodontitis (CP) in vitro. We established a periodontitis cell model was established by Porphyromonas gingivalis lipopolysaccharide (Pg-LPS)-induced periodontal ligament cells (PDLCs). Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to detect the expression of NEAT1, microRNA (miR)-200c-3p, and tumor necrosis factor receptor-associated factor 6 (TRAF6). Cell viability, inflammatory factors, and protein expression of Bcl-2, Bax, and TRAF6 were analyzed by MTT, enzyme-linked immunosorbent assay, and Western blot. The target relationships among NEAT1, miR-200c-3p, and TRAF6 were predicted by the StarBase/TargetScan software, and further validated by dual-luciferase reporter assay. In this research, NEAT1 is up-regulated in CP tissues and periodontitis model group. Silencing of NEAT1 and over-expression of miR-200c-3p enhanced cell viability and repressed apoptosis in the periodontitis model group. NEAT1 targets miR-200c-3p, and miR-200c-3p further targets TRAF6. MiR-200c-3p inhibitor or over-expression of TRAF6 reversed the promoting effect of NEAT1 knockdown on cell viability, and the inhibiting effects on inflammatory cytokines and cell apoptosis. Consequently, the silencing of NEAT1 inhibits inflammation and apoptosis via targeting miR-200c-3p/TRAF6 axis, thereby contributing to alleviate the progression of CP. This finding could provide an underlying target for the treatment of CP.

[Histopathological study of the effects of intra-articular injection of triamcinolone acetonide on temporomandibular joint osteoarthrosis in goats].

PURPOSE: The aim of this study was to investigate the histopathological changes of human temporomandibular joint osteoarthrosis following intra-articular injection of triamcinolone acetonide, and compare the results to these of control group. METHODS: The upper compartments of both joints of 10 goats were injected with 0.8 ml collagenase I to induce osteoarthrosis. The 10 goats were then equally divided into control group and experimental group. The first and second treatment were carried out 30 and 60 days after injection of collagenase I, respectively. The animals were sacrificed 90 days after the first treatment. The specimens of the animal TMJ were harvested, histologically examined under light microscopy and scanning electron microscopy, and scored in a subjective manner following the criteria which was a modification of the method of Mankin et al and Yoshimi et al. GLM model in SAS software package was adopted to compare the differences between the control group and experimental group. RESULTS: Under light microscopy and scanning electron microscopy, the condyle, meniscus and glenoid fossa of the control group demonstrated distinct osteoarthrosis; the histopathologic changes in the experimental group was more serious than the control group, and the differences of the histologic scores were significant between the two groups (P<0.01). CONCLUSION: Intra-articular injection of triamcinolone acetonide as a lytic agent will worsen for articular cartilage reparation.

[Clinical study of bioglass for repairing alveolar cleft.].

PURPOSE: To analyse the results of using Bioglass to repair alveolar cleft. METHODS: Thirty-nine cases with alveolar cleft were divided into two groups. In group A, autogenous iliac cancellous bone were transplanted into the cleft to repair alveolar defect in 25 cases, while artificial bone-Bioglass was used in group B of 14 cases. The results of two groups were compared with a follow up of 12 months. RESULTS: New bone formation was perfect in grafted area and canines can emerge from the bone grafted areas observed from the X-ray films both in group A and group B. Statistical analysis showed no significant difference between group A and group B in the clinical success rate of alveolar cleft repair. However, there was significant difference between complete alveolar cleft and incomplete alveolar cleft in group A and group B. CONCLUSION: Bioglass can result in new bone formation and eruption of canines from the bone grafted areas. The application of Bioglass in repairing of alveolar cleft provides a new therapy for alveolar cleft patients.