RESUMO
With the increasing demand for trace sample analysis, injecting trace samples into liquid chromatography-mass spectrometry (LC-MS) systems with minimal loss has become a major challenge. Herein, we describe an in situ LC-MS analytical probe, the Falcon probe, which integrates multiple functions of high-pressure sample injection without sample loss, high-efficiency LC separation, and electrospray. The main body of the Falcon probe is made of stainless steel and fabricated by the computer numerical control (CNC) technique, which has ultrahigh mechanical strength. By coupling a nanoliter-scale droplet reactor made of polyether ether ketone (PEEK) material, the Falcon probe-based LC-MS system was capable of operating at mobile-phase pressures up to 800 bar, which is comparable to those of conventional ultraperformance liquid chromatography (UPLC) systems. Using the probe pressing microamount in situ (PPMI) injection approach, the Falcon probe-based LC-MS system showed high separation efficiency and good repeatability with relative standard deviations (RSDs) of retention time and peak area of 1.8% and 9.9%, respectively, in peptide mixture analysis (n = 6). We applied this system to the analysis of a trace amount of 200 pg of HeLa protein digest and successfully identified an average of 766 protein groups (n = 5). By combining in situ sample pretreatment at the nanoliter range, we further applied the present system in single-cell proteomic analysis, and 241 protein groups were identified in single 293 cells, which preliminarily demonstrated its potential in the analysis of trace amounts of samples with complex compositions.
Assuntos
Pressão , Humanos , Cromatografia Líquida/métodos , Espectrometria de Massas/métodos , Nanotecnologia , Polietilenoglicóis/química , Peptídeos/análise , Cromatografia Líquida de Alta Pressão , Células HeLa , Benzofenonas/análise , Benzofenonas/química , Polímeros/química , Cetonas/química , Cetonas/análise , Proteômica/métodosRESUMO
Characterizing the profiles of proteome and metabolome at the single-cell level is of great significance in single-cell multiomic studies. Herein, we proposed a novel strategy called one-shot single-cell proteome and metabolome analysis (scPMA) to acquire the proteome and metabolome information in a single-cell individual in one injection of LC-MS/MS analysis. Based on the scPMA strategy, a total workflow was developed to achieve the single-cell capture, nanoliter-scale sample pretreatment, one-shot LC injection and separation of the enzyme-digested peptides and metabolites, and dual-zone MS/MS detection for proteome and metabolome profiling. Benefiting from the scPMA strategy, we realized dual-omic analysis of single tumor cells, including A549, HeLa, and HepG2 cells with 816, 578, and 293 protein groups and 72, 91, and 148 metabolites quantified on average. A single-cell perspective experiment for investigating the doxorubicin-induced antitumor effects in both the proteome and metabolome aspects was also performed.
Assuntos
Proteoma , Espectrometria de Massas em Tandem , Humanos , Proteoma/metabolismo , Cromatografia Líquida , Metaboloma , Células HeLaRESUMO
The shotgun proteomic analysis is currently the most promising single-cell protein sequencing technology, however its identification level of ~1000 proteins per cell is still insufficient for practical applications. Here, we develop a pick-up single-cell proteomic analysis (PiSPA) workflow to achieve a deep identification capable of quantifying up to 3000 protein groups in a mammalian cell using the label-free quantitative method. The PiSPA workflow is specially established for single-cell samples mainly based on a nanoliter-scale microfluidic liquid handling robot, capable of achieving single-cell capture, pretreatment and injection under the pick-up operation strategy. Using this customized workflow with remarkable improvement in protein identification, 2449-3500, 2278-3257 and 1621-2904 protein groups are quantified in single A549 cells (n = 37), HeLa cells (n = 44) and U2OS cells (n = 27) under the DIA (MBR) mode, respectively. Benefiting from the flexible cell picking-up ability, we study HeLa cell migration at the single cell proteome level, demonstrating the potential in practical biological research from single-cell insight.
Assuntos
Proteoma , Proteômica , Animais , Humanos , Células HeLa , Proteômica/métodos , Proteoma/metabolismo , Análise de Célula Única , Fluxo de Trabalho , Mamíferos/metabolismoRESUMO
Although single-cell multi-omics technologies are undergoing rapid development, simultaneous transcriptome and proteome analysis of a single-cell individual still faces great challenges. Here, we developed a single-cell simultaneous transcriptome and proteome (scSTAP) analysis platform based on microfluidics, high-throughput sequencing, and mass spectrometry technology to achieve deep and joint quantitative analysis of transcriptome and proteome at the single-cell level, providing an important resource for understanding the relationship between transcription and translation in cells. This platform was applied to analyze single mouse oocytes at different meiotic maturation stages, reaching an average quantification depth of 19,948 genes and 2,663 protein groups in single mouse oocytes. In particular, we analyzed the correlation of individual RNA and protein pairs, as well as the meiosis regulatory network with unprecedented depth, and identified 30 transcript-protein pairs as specific oocyte maturational signatures, which could be productive for exploring transcriptional and translational regulatory features during oocyte meiosis.
Assuntos
Proteoma , Transcriptoma , Animais , Camundongos , Transcriptoma/genética , Proteoma/metabolismo , Oócitos/metabolismo , Oogênese/genética , Perfilação da Expressão Gênica , MeioseRESUMO
Herein, we describe an in situ analysis probe, Petrel probe, highly integrating multiple functions of in situ sampling, in situ sample injection, high-performance liquid chromatography (HPLC) separation, and mass spectrometry (MS) electrospray. The Petrel probe was fabricated based on a single capillary, which consists of a micrometer-sized hole for sampling, a packed column for LC separation, and a tapered tip for MS electrospray. The design of the Petrel probe was optimized to obtain higher structural strength, and a polytetrafluoroethylene (PTFE) chip was used for sealing the probe-sampling hole to meet the high-pressure (â¼30 MPa) requirement of LC manifold. On the basis of the Petrel probe, we developed a novel valveless LC injection method, that is, the probe pressing microamount in situ (PPMI) injection method, which performs sample injection by pressing the probe-sampling hole on the PTFE chip, using the mobile phase to dissolve the sample dry spot in the sampling area on the chip, and injecting it into the LC column under high-pressure conditions for separation and subsequent MS analysis. The LC-MS system with the PPMI injection method exhibits rapid injection and separation speed, as well as minimum injection dead volume. It can yield a high separation efficiency comparable to those of conventional HPLC systems. The present system was optimized using standard peptide samples, and four peptides were separated within 11 min in a probe with an effective column length of 5 cm, achieving the highest theoretical plate number up to â¼5,500,000/m. The system was also applied in the separation of cytochrome C digest to demonstrate its separation ability for complex samples, and 21 peptides were detected in 8 min with an amino-acid coverage of 83%.
Assuntos
Peptídeos , Espectrometria de Massas por Ionização por Electrospray , Animais , Aves , Cromatografia Líquida de Alta Pressão , Cromatografia LíquidaRESUMO
In situ sampling mass spectrometry (MS) systems can achieve rapid analysis of samples, while most of them do not have the pretreatment capability of chromatographic separation. This Article describes the design, fabrication, and application of a swan-shaped in situ sampling MS probe with liquid chromatography (LC) separation capacity. The LC-Swan probe was fabricated based on a single capillary with a micrometer-sized hole at its U-shaped bottom for sampling, a monolithic column for separation, and a tapered tip for electrospray. Four functions including in situ sampling, sample injection, chromatographic separation, and MS electrospray were integrated in the LC-Swan probe. Direct sampling and contacting-dissolution-injection sampling modes were developed to perform in situ sampling and injection of liquid samples and dry spot samples, respectively, in the high flow-resistance LC system. A pressing-sealing method was also developed using a polydimethylsiloxane (PDMS) sealer to achieve the sealing of the probe sampling hole during the high-pressure chromatographic separation process. The LC-Swan probe-based system exhibited effective desalting capacity in the analysis of angiotensin II with similar relative standard deviations (RSDs) of retention time and peak area below 3% and 19% (n = 3) for both salt-containing and salt-free samples. The present system was applied for analyzing cytochrome C digest to test its separation capability for samples with complex compositions, and 19 peptides were detected in 13 min with an amino acid coverage of 85%. We also applied the system in metabolite analysis of mouse organ sections of brain, liver, and kidney to preliminarily demonstrate its application potential in MS imaging analysis.