Optimization of the spatial layout of animal husbandry based on comprehensive competitive advantage evaluation and nutrient balance: a case study of Heilongjiang province.

The optimization of the spatial layout of animal husbandry has great significance for the sustainable development of animal husbandry. However, limited research has focused on the optimization of the spatial layout of animal husbandry based on comprehensive competitive advantage evaluation and nutrient balance between planting and breeding. This study established a comprehensive competitive advantage evaluation model of animal husbandry. Based on this model, we performed comprehensive competitive advantage evaluation of animal husbandry. We further calculated the land-carrying capacity index of animal manure and potential land-carrying capacity of animal according to the principle of nutrient balance. Finally, the spatial layout of animal husbandry was optimized using Geographic Information System spatial analysis. Results showed that Harbin had obvious comprehensive competitive advantage in pig, cattle, sheep, and poultry breeding. Daqing had obvious comprehensive competitive advantage in cattle and sheep breeding. The land-carrying capacity index of animal manure of Daxing'anling constantly exceeded 1. The potential land-carrying capacity of animal in Heilongjiang province was uneven, and most regions in Heilongjiang province had development potential of animal husbandry. The optimized target regions of the spatial layout of pig, cattle, sheep, and poultry breeding were all Harbin. A total of 77 956.03 pig equivalent in Daxing'anling was recommended to be transferred to Harbin. Our results are useful for the spatial layout of animal husbandry, environmental pollution prevention from animal husbandry, and optimization of animal products structure.

[Impact of Climate Warming on Paddy Soil Organic Carbon Change in the Sichuan Basin of China].

Climate warming can increase soil temperature and lead to soil carbon release, but it can also increase soil organic carbon by increasing primary productivity. Cropland soils are considered to have a huge potential to sequester carbon; however, direct observations for the responses of cropland soil organic carbon to climate warming over broad geographic scales are rarely documented. Paddy soil is one of the important cultivated soils in China. Based on the data of 2217 sampling points obtained during the second national soil survey and the data of 2382 sampling points collected during 2017-2019, this study analyzed the change characteristics of soil organic carbon content of paddy surface soil in Sichuan Basin of China and explored the relationships between the soil organic carbon change of paddy soil and temperature, precipitation, cropland use type, fertilization intensity, and grain yield. The results showed that the content of soil organic carbon of paddy soil changed from 13.33 g·kg-1to 15.96 g·kg-1, with an increase of 2.63 g·kg-1, suggesting that soils in the Sichuan Basin have acted as a carbon sink over past 40 years. The soil organic carbon increment of paddy soil varied with different geomorphic regions and different secondary basins. The increase in SOC content in paddy soil was positively correlated with annual average temperature; negatively correlated with annual average precipitation; and initially increased and then decreased with annual average fertilizer application, annual average increase rate of fertilizer application, annual average grain yield, and annual average grain yield growth rate. The relationship between the increase in SOC content and the annual average temperature growth rate was different under different farmland utilizations, and the increase in the annual average temperature growth rate had significant effects with the increase in SOC content only on paddy-dryland rotation. These results indicate that the paddy soil organic carbon change in Sichuan Basin was co-affected by various factors, but climate warming was an important factor leading to the paddy soil organic carbon change, and its influence was controlled by the water conditions determined by farmland use.

Identification of livestock farms with potential risk of environmental pollution by using a model for returning livestock manure to cultivated land.

Livestock farms produce a large amount of livestock manure, which, if not disposed of properly, will become an environmental pollution source. However, limited research has been conducted on identifying livestock farms with a potential risk of environmental pollution. In this study, a model for returning livestock manure to cultivated land was constructed. Livestock manure was returned to cultivated land, and the amount of surplus livestock manure nutrients, including nitrogen and phosphorus, in livestock farms was calculated with pig manure as an example. Then, livestock farms with a potential risk of environmental pollution in Shanggan Town and part of Xiangqian Town in Fuzhou City were identified using phosphorus as an index. Results showed that the spatial distributions of nitrogen and phosphorus from pig manure in the cultivated land varied. All cultivated lands exhibited the maximum carrying capacity for phosphorus, and 1811 cultivated lands exhibited potential nitrogen carrying capacity. A total of 13,958.997 kg of phosphorus from pig manure in 144 livestock farms could not be disposed to cultivated lands. In all, 144 livestock farms with surplus phosphorus from pig manure were identified as potential environmental pollution sources. These findings can serve as a scientific basis for the utilization of livestock manure, prevention of environmental pollution caused by livestock farms, and layout planning of livestock farms.

Potential reduction of greenhouse gas emissions from pig production in China on the basis of households' pork consumption.

In the past decades, the greenhouse gas (GHG) emissions from pig production in China have been increasing rapidly, which has become a huge challenge in fulfilling China's "carbon neutral" commitment. However, few studies have focused on reducing the GHG emissions from pig production in view of households' pork consumption. This study analyzed the temporal and spatial pattern of the GHG emissions from pig production in China in 2001-2020 through geographical information system, optimized the pig production in China, and estimated thepotentialGHG emissions reduction from pig production in China in 2020 through spatial analysis based on pork surplus or deficit. Results show that the temporal and spatial pattern of the GHG emissions from pig production and its proportion in the total GHG emissions from livestock production in China in 2001-2020 varied differently at the province level and conformed to the "Hu Huanyong Line" mode. The largest and smallest GHG emissions from pig production were 108.93 million tons (MT) in 2014 and 78.10 MT in 2020, respectively. The largest and smallest proportions of GHG emissions from pig production in the total GHG emissions from livestock production were 77.52% in Zhejiang in 2013 and 0.13% in Tibet in 2009, respectively. Moreover, a potential optimization scheme of pig production in China in 2020 was provided and a method of GHG emissions reduction from pig production is proposed. The results indicate that the total potentialGHG emissions reduction from pig production on the basis of households' pork consumption could reach 35.21 MT, accounting for 45.09% of the total GHG emissions from pig production and 10.27% of the total GHG emissions from livestock production in China in 2020. These findings areusefulin the spatial layout planning of pig production, agricultural GHG reduction, and global warming mitigation.

Biogas potential from agricultural waste and its CO2 emission reduction: a case study of Hubei Province, China.

Biogas produced from agricultural waste can have potential benefits, such as offer clean renewable energy, protect the ecological environment, and reduce CO2 emission. However, few studies have been conducted on the biogas potential from agricultural waste and its CO2 emission reduction at the county level. Herein, the biogas potential from agricultural waste was calculated, and its spatial distribution in Hubei Province in 2017 was determined using a geographic information system. Then, an evaluation model for the competitive advantage of the biogas potential from agricultural waste was established using entropy weight and linear weighting methods. Moreover, the space partition of the biogas potential from agricultural waste was obtained through hot spot analysis. Lastly, the standard coal equivalent of biogas, the equivalent of coal consumption of biogas, and the CO2 emission reduction based on the space partition result were estimated. Results showed that the total and average biogas potentials from agricultural waste in Hubei Province were 18,498,317,558.54 and 222,871,295.89 m3, respectively. Qianjiang City, Jianli County, Xiantao City, and Zaoyang City had a high competitive advantage in the biogas potential from agricultural waste. The CO2 emission reduction of the biogas potential from agricultural waste was mainly in classes I and II.

Addition of Organic Acids and Lactobacillus acidophilus to the Leguminous Forage Chamaecrista rotundifolia Improved the Quality and Decreased Harmful Bacteria of the Silage.

This study aimed to investigate the effects of citric acid, malic acid, and Lactobacillus acidophilus (L) on fermentation parameters and the microbial community of leguminous Chamaecrista rotundifolia silage. Fresh C. rotundifolia was treated without any additive (CK), or with L (106 CFU/g fresh weight), different levels (0.1, 0.3, 0.5, and 1% fresh weight) of organic acid (malic or citric acid), and the combinations of L and the different levels of organic acids for 30, 45, and 60 days of ensiling. The effects of malic acid and citric acid were similar during the ensiling process. Treatment with either citric or malic acid and also when combined with L inhibited crude protein degradation, lowered pH and ammonia nitrogen, and increased lactic acid concentration and dry matter content (p < 0.05). The neutral detergent fiber and acid detergent fiber increased initially and then decreased with fermentation time in all treatments (p < 0.05). Increasing the level of organic acid positively affected the chemical composition of C. rotundifolia silage. In addition, the addition of 1% organic acid increased the relative abundance of Lactobacillus, while the relative abundances of Clostridium and Enterobacter decreased at 60 days (p < 0.05). Moreover, both organic acids and combined additives increased (p < 0.05) the relative abundance of Cyanobacteria at 60 days of fermentation. We concluded that adding malic acid, citric acid, and L combined with an organic acid could improve the quality of C. rotundifolia silage and increase the relative abundance of beneficial bacteria. The addition of organic acid at a level of 1% was the most effective.

Divergent responses of cropland soil organic carbon to warming across the Sichuan Basin of China.

Cropland soils are considered to have the potential to sequester carbon (C). Warming can increase soil organic C (SOC) by enhancing primary production, but it can also cause carbon release from soils. However, the role of warming in governing cropland SOC dynamics over broad geographic scales remains poorly understood. Using over 4000 soil samples collected in the 1980s and 2010s across the Sichuan Basin of China, this study assessed the warming-induced cropland SOC change and the correlations with precipitation, cropland type and soil type. Results showed mean SOC content increased from 11.10 to 13.85 g C kg-1. Larger SOC increments were observed under drier conditions (precipitation < 1050 mm, dryland and paddy-dryland rotation cropland), which were 1.67-2.23 times higher than under wetter conditions (precipitation > 1050 mm and paddy fields). Despite the significant associations of SOC increment with crop productivity, precipitation, fertilization, cropland type and soil type, warming also acted as one of major contributors to cropland SOC change. The SOC increment changed parabolically with the rise in temperature increase rate under relatively drier conditions, while temperature increase had no impact on cropland SOC increment under wetter conditions. Meanwhile, the patterns of the parabolical relationship varied with soil types in drylands, where the threshold of temperature increase rate, the point at which the SOC increment switched from increasing to decreasing with warming, was lower for clayey soils (Ali-Perudic Argosols) than for sandy soils (Purpli-Udic Cambosols). These results illustrate divergent responses of cropland SOC to warming under different environments, which were contingent on water conditions and soil types. Our findings emphasize the importance of formulating appropriate field water management for sustainable C sequestration and the necessity of incorporating environment-specific mechanisms in Earth system models for better understanding of the soil C-climate feedback in complex environments.

Spatial-temporal distribution of biogas production from agricultural waste per capita in rural China and its correlation with ground temperature.

Biogas production from agricultural waste has played a key role in recent years in reducing the environmental pollution risk of agricultural waste and alleviating energy shortage in rural China. However, few studies have focused on the effect of ground temperature on biogas production from agricultural waste from a quantitative point of view and the solution to increasing the temperature of biogas digesters in the cold season to improve biogas production from agricultural waste in rural areas at a provincial scale. Here, we calculated the spatial-temporal distribution of biogas production from agricultural waste per capita in rural China from 2001 to 2016 and agricultural waste per capita in rural China in 2016. Furthermore, we analyzed the correlation between biogas production from agricultural waste per capita in rural China and ground temperature. Finally, we proposed a solution to increase the temperature of biogas digesters in rural China in the cold season after analyzing its energy utilization. Results showed that the biogas production from agricultural waste per capita in rural China was correlated with the average and maximum ground temperature in January, February, March, November, and December from 2001 to 2016. The results also indicated that the suggested heating mode of biogas production from agricultural waste in rural China differed greatly among provinces. The provinces with high agricultural waste per capita in rural areas and low averaged biogas production from agricultural waste per capita are Inner Mongolia, Heilongjiang, and Xinjiang, which needed the suggested heating mode of biogas production from agricultural waste for increasing the temperature of biogas digesters in rural China in the cold season. Our findings provide a reference for biogas production from agricultural waste, greenhouse gas emission reduction, and the spatial layout of energy utilization in rural China.

Effect of sympathetic sprouting on the excitability of dorsal root ganglion neurons and afferents in a rat model of neuropathic pain.

Increased sympathetic nerve excitability has been reported to aggravate a variety of chronic pain conditions, and an increase in the number of sympathetic nerve fibers in the dorsal root ganglion (DRG) has been found in neuropathic pain (NP) models. However, the mechanism of the neurotransmitter norepinephrine (NE) released by sympathetic nerve fiber endings on the excitability of DRG neurons is still controversial, and the adrenergic receptor subtypes involved in this biological process are also controversial. In our study, we have two objectives: (1) To determine the effect of the neurotransmitter NE on the excitability of different neurons in DRG; (2) To determine which adrenergic receptors are involved in the excitability of DRG neurons by NE released by sprouting sympathetic fibers. In this experiment, a unique field potential recording method of spinal cord dorsal horn was innovatively adopted, which can be used for electrophysiological study in vivo. The results showed that： Forty days after SNI, patch clamp and field potential recording methods confirmed that NE enhanced the excitability of ipsilateral DRG large neurons, and then our in vivo electrophysiological results showed that the α2 receptor blocker Yohimbine could block the excitatory effect of NE on A-fiber and the inhibitory effect on C-fiber, while the α2A-adrenergic receptor agonist guanfacine (100 µM) had the same biological effect as NE. Finally, we concluded that NE from sympathetic fiber endings is involved in the regulation of pain signaling by acting on α2A-adrenergic receptors in DRG.

Adipose tissue alleviates the stress response by releasing adiponectin during laparoscopic surgery in patients with colorectal cancer.

BACKGROUND AND OBJECTIVES: Laparoscopic colorectal surgery causes a lower stress response than open surgery. Adiponectin is mainly derived from adipocytes and has antidiabetic, antioxidative, and anti-inflammatory capabilities. The objective of the present study was to investigate the protein expression of adiponectin in adipose tissue, and the serum levels of adiponectin, oxidative stress markers and proinflammatory factors during laparoscopic colorectal surgery and open surgery periods. METHODS: Forty patients aged 60 to 80, with American Society of Anesthesiologists (ASA) I ~ II who underwent radical resection of colorectal cancer were recruited to the study. Laparoscopic group and open group included 20 patients each. Mesenteric adipose tissue and venous blood before (T1) and at the end (T2) of surgery were collected to examine adiponectin levels, and venous blood was collected to examine serum levels of oxidative stress related markers (superoxide dismutase (SOD), glutathione (GSH), lipid peroxide (LPO), malondialdehyde (MDA)), and inflammation-related factors (interleukin (IL)-1ß, interleukin (IL)-6, tumor necrosis factor-α (TNF-α)). RESULTS: Protein and serum levels of adiponectin were analyzed, and adiponectin levels were significantly increased at T2 than T1 in the laparoscopic surgery, while adiponectin levels were significantly higher in the laparoscopic surgery than in the open surgery at T2. In addition, the serum levels of SOD and GSH were significantly higher in the laparoscopic surgery than in open surgery at T2. However, the serum levels of LPO, TNF-α, IL-1ß, and IL-6 were significantly lower in the laparoscopic group than in open group at T2. CONCLUSION: Laparoscopic surgery induced higher levels of adiponectin in both adipose tissue and the bloodstream. Oxidative stress and the inflammatory response were lower during laparoscopic colorectal surgery than during conventional open surgery. These data suggest that adipose tissue may alleviate the stress response during laparoscopic surgery by releasing adiponectin in patients with colorectal cancer.

Dexmedetomidine attenuates H2O2-induced apoptosis of rat cardiomyocytes independently of antioxidant enzyme expression.

INTRODUCTION AND OBJECTIVES: Dexmedetomidine is a highly selective alpha-2 adrenoceptor agonist that has sedative and analgesic properties and myocardial protective effects. However, the mechanism underlying the protective effect of dexmedetomidine on cardiomyocytes remains unknown. This study mainly aimed to investigate the effects of dexmedetomidine on the generation of reactive oxygen species (ROS) in cardiomyocytes and whether it inhibits the apoptosis of cardiomyocytes by affecting antioxidant enzyme expression. METHODS: Neonatal rat cardiomyocytes were pretreated with dexmedetomidine (100 nM) for 24 h. The cardiomyocytes were then incubated with 200 µM hydrogen peroxide solution (H2O2) for 4 h. PCR assay was used to determine the mRNA expression of antioxidant enzymes. Western blot assay was used to determine the protein expression of antioxidant enzymes. Fluorescence microscopy with the MitoSOX probe was used to detect the formation of ROS in cardiomyocytes, and fluorescence-activated cell sorting with annexin V/PI was used to determine the number of apoptotic cardiomyocytes. RESULTS: Dexmedetomidine reduced ROS generation and antioxidant enzymes levels in cardiomyocytes before H2O2 stimulation (p<0.05). However, ROS generation and apoptosis in cardiomyocytes were significantly increased after H2O2 treatment, and dexmedetomidine pretreatment markedly inhibited the changes (p<0.05). CONCLUSION: For the first time, to the best of our knowledge, our study shows that dexmedetomidine has a protective effect on cardiomyocytes through inhibition of ROS-induced apoptosis, and more importantly, this effect is independent of antioxidant enzyme mRNA and protein expression.

Random forest model based fine scale spatiotemporal O3 trends in the Beijing-Tianjin-Hebei region in China, 2010 to 2017.

Ambient ozone (O3) concentrations have shown an upward trend in China and its health hazards have also been recognized in recent years. High-resolution exposure data based on statistical models are needed. Our study aimed to build high-performance random forest (RF) models based on training data from 2013 to 2017 in the Beijing-Tianjin-Hebei (BTH) region in China at a 0.01 ° × 0.01 ° resolution, and estimated daily maximum 8h average O3 (O3-8hmax) concentration, daily average O3 (O3-mean) concentration, and daily maximum 1h O3 (O3-1hmax) concentration from 2010 to 2017. Model features included meteorological variables, chemical transport model output variables, geographic variables, and population data. The test-R2 of sample-based O3-8hmax, O3-mean and O3-1hmax models were all greater than 0.80, while the R2 of site-based and date-based model were 0.68-0.87. From 2010 to 2017, O3-8hmax, O3-mean, and O3-1hmax concentrations in the BTH region increased by 4.18 µg/m3, 0.11 µg/m3, and 4.71 µg/m3, especially in more developed regions. Due to the influence of weather conditions, which showed high contribution to the model, the long-term spatial distribution of O3 concentrations indicated a similar pattern as altitude, where high concentration levels were distributed in regions with higher altitude.

Transcriptome profiling reveals the effects of drought tolerance in Giant Juncao.

BACKGROUND: Giant Juncao is often used as feed for livestock because of its huge biomass. However, drought stress reduces forage production by affecting the normal growth and development of plants. Therefore, investigating the molecular mechanisms of drought tolerance will provide important information for the improvement of drought tolerance in this grass. RESULTS: A total of 144.96 Gb of clean data was generated and assembled into 144,806 transcripts and 93,907 unigenes. After 7 and 14 days of drought stress, a total of 16,726 and 46,492 differentially expressed genes (DEGs) were observed, respectively. Compared with normal irrigation, 16,247, 23,503, and 11,598 DEGs were observed in 1, 5, and 9 days following rehydration, respectively. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses revealed abiotic stress-responsive genes and pathways related to catalytic activity, methyltransferase activity, transferase activity, and superoxide metabolic process. We also identified transcription factors belonging to several families, including basic helix-loop-helix (bHLH), WRKY, NAM (no apical meristem), ATAF1/2 and CUC2 (cup-shaped cotyledon) (NAC), fatty acyl-CoA reductase (FAR1), B3, myeloblastosis (MYB)-related, and basic leucine zipper (bZIP) families, which are important drought-rehydration-responsive proteins. Weighted gene co-expression network analysis was also used to analyze the RNA-seq data to predict the interrelationship between genes. Twenty modules were obtained, and four of these modules may be involved in photosynthesis and plant hormone signal transduction that respond to drought and rehydration conditions. CONCLUSIONS: Our research is the first to provide a more comprehensive understanding of DEGs involved in drought stress at the transcriptome level in Giant Juncao with different drought and recovery conditions. These results may reveal insights into the molecular mechanisms of drought tolerance in Giant Juncao and provide diverse genetic resources involved in drought tolerance research.

Crop yield and production responses to climate disasters in China.

In recent decades, climate disasters, especially droughts and floods, have disrupted food production and caused yield losses. Crop models cannot identify quantitative changes of crop yield and production caused by climate disasters directly, and empirical approaches possibly underestimate the effects due to the use of the entire world or an individual nation as the unit of study. Here, we quantified the losses of crop yield and production resulting from droughts and floods across China during 1982-2012 using the superposed epoch analysis (SEA) method. We defined the actual-occurred and control composited series using provincial-level data in China, i.e., covered area (CA), affected area (AA), and sown area (SA) from 1982 to 2012, and crop yields and production of maize, rice, soybean, and wheat from 1979 to 2015. The results showed that maize and soybean in China suffered serious damage from droughts, with reductions in yields and production ranging from 7.8% to 11.6% between 1982 and 2012. Droughts and floods significantly decreased wheat yield by 5.8% and 6.1%, respectively. Moreover, rice yield and production were sensitive to both droughts and floods, with reductions of 4.5-6.3%. Among the MGPA, crops cultivated in NEC and the Huang-Huai-Hai Plain (HHH) were more easily affected by droughts. Among the four main crops and the three types of rice in the mid-lower reaches of the Yangtze River (MLYR), the yield and production of only early rice were sensitive to floods. The quantitative identification of the spatial responses of crop yields and production to droughts and floods can help us better understand the impacts of climate disasters on food security in China and the whole world, which is essential for addressing potential adaptation strategies.

The protective effect of recombinant globular adiponectin on testis by modulating autophagy, endoplasmic reticulum stress and oxidative stress in streptozotocin-induced diabetic mice.

This study was to investigate whether recombinant globular adiponectin produced its protective effect on the testis of diabetic mice by modulating autophagy, endoplasmic reticulum stress and oxidative stress. Male mice were randomly divided into control, diabetic, diabetic treated with low and high dose of adiponectin. Mice were killed at the termination after 4 weeks and 8 weeks of adiponectin treatment. Serum levels of glucose, lipids, testosterone, insulin, LH and FSH were measured. The protein expression of glucose-regulated protein 78 (GRP78), C/EBP homologous protein (CHOP), Caspase12, Beclin1, microtubule-associated protein light chain 3 (LC3) and p62 was determined by western blotting. The mRNA expression of adiponectin receptor 1 (AdipoR1), p22phox, p47phox, nuclear factor erythroid2-related factor 2 (Nrf2), NAD(P)H-quinone oxidoreductase 1(NQO1), heme oxygenase-1 (HO-1) and superoxide dismutase (SOD) were determined by real-time fluorescence quantitative PCR. The testicular weight, the sperm number and motility, and the serum levels of testosterone and insulin were significantly decreased in diabetic mice (P < 0.05). The expression of Beclin1, LC3, Nrf2, NQO1, HO-1, SOD and AdipoR1 were significantly decreased (P < 0.05), while the expression of GRP78, CHOP, Caspase12, p62, p22phox and p47phox were notably increased in the testes of diabetic mice (P < 0.05). Adiponectin treatment significantly reversed the above-mentioned changes in the testes of diabetic mice, some of which were dose- and time-dependent (P < 0.05). These data suggested that recombinant globular adiponectin may produce the protective effect on the testes of diabetic mice by inducing autophagy and inhibiting ER stress and oxidative stress.

Testicular Injury Attenuated by Rapamycin Through Induction of Autophagy and Inhibition of Endoplasmic Reticulum Stress in Streptozotocin- Induced Diabetic Rats.

BACKGROUND AND OBJECTIVE: This study investigated whether rapamycin has a protective effect on the testis of diabetic rats by regulating autophagy, endoplasmic reticulum stress, and oxidative stress. METHODS: Thirty male Sprague-Dawley rats were randomly divided into three groups: control, diabetic, and diabetic treated with rapamycin, which received gavage of rapamycin (2mg.kg-1.d-1) after induction of diabetes. Diabetic rats were induced by intraperitoneal injection of streptozotocin (STZ, 65mg.Kg-1). All rats were sacrificed at the termination after 8 weeks of rapamycin treatment. The testicular pathological changes were determined by hematoxylin and eosin staining. The protein or mRNA expression of autophagy-related proteins (Beclin1, microtubule-associated protein light chain 3 (LC3), p62), ER stress marked proteins (CCAAT/enhancer-binding protein (C/EBP) homologous protein (CHOP), caspase-12), oxidative stress-related proteins (p22phox, nuclear factor erythroid2-related factor 2 (Nrf2)) and apoptosis-related proteins (Bax, B cell lymphoma-2 (Bcl-2)) were assayed by western blot or real-time fluorescence quantitative PCR. RESULTS: There were significant pathological changes in the testes of diabetic rats. The expression of Beclin1, LC3, Nrf2, Bcl-2 were significantly decreased and p62, CHOP, caspase12, p22phox, and Bax were notably increased in the testis of diabetic rats (P <0.05). However, rapamycin treatment for 8 weeks significantly reversed the above changes in the testis of diabetic rats (P <0.05). CONCLUSION: Rapamycin appears to produce a protective effect on the testes of diabetic rats by inducing the expression of autophagy and inhibiting the expression of ER-stress, oxidative stress, and apoptosis.

Prediction of soil cadmium distribution across a typical area of Chengdu Plain, China.

A suitable method and appropriate environmental variables are important for accurately predicting heavy metal distribution in soils. However, the classical methods (e.g., ordinary kriging (OK)) have a smoothing effect that results in a tendency to neglect local variability, and the commonly used environmental variables (e.g., terrain factors) are ineffective for improving predictions across plains. Here, variables were derived from the obvious factors affecting soil cadmium (Cd), such as road traffic, and were used as auxiliary variables for a combined method (HASM_RBFNN) that was developed using high accuracy surface modelling (HASM) and radial basis function neural network (RBFNN) model. This combined method was then used to predict soil Cd distribution in a typical area of Chengdu Plain in China, considering the spatial non-stationarity of the relationships between soil Cd and the derived variables based on 339 surface soil samples. The results showed that HASM_RBFNN had lower prediction errors than OK, regression kriging (RK) and HASM_RBFNNs, which didn't consider the spatial non-stationarity of the soil Cd-derived variables relationships. Furthermore, HASM_RBFNN provided improved detail on local variations. The better performance suggested that the derived environmental variables were effective and HASM_RBFNN was appropriate for improving the prediction of soil Cd distribution across plains.

Surface modeling of soil antibiotics.

Large numbers of livestock and poultry feces are continuously applied into soils in intensive vegetable cultivation areas, and then some veterinary antibiotics are persistent existed in soils and cause health risk. For the spatial heterogeneity of antibiotic residues, developing a suitable technique to interpolate soil antibiotic residues is still a challenge. In this study, we developed an effective interpolator, high accuracy surface modeling (HASM) combined vegetable types, to predict the spatial patterns of soil antibiotics, using 100 surface soil samples collected from an intensive vegetable cultivation area located in east of China, and the fluoroquinolones (FQs), including ciprofloxacin (CFX), enrofloxacin (EFX) and norfloxacin (NFX), were analyzed as the target antibiotics. The results show that vegetable type is an effective factor to be combined to improve the interpolator performance. HASM achieves less mean absolute errors (MAEs) and root mean square errors (RMSEs) for total FQs (NFX+CFX+EFX), NFX, CFX and EFX than kriging with external drift (KED), stratified kriging (StK), ordinary kriging (OK) and inverse distance weighting (IDW). The MAE of HASM for FQs is 55.1 µg/kg, and the MAEs of KED, StK, OK and IDW are 99.0 µg/kg, 102.8 µg/kg, 106.3 µg/kg and 108.7 µg/kg, respectively. Further, RMSE simulated by HASM for FQs (CFX, EFX and NFX) are 106.2 µg/kg (88.6 µg/kg, 20.4 µg/kg and 39.2 µg/kg), and less 30% (27%, 22% and 36%), 33% (27%, 27% and 43%), 38% (34%, 23% and 41%) and 42% (32%, 35% and 51%) than the ones by KED, StK, OK and IDW, respectively. HASM also provides better maps with more details and more consistent maximum and minimum values of soil antibiotics compared with the measured data. The better performance can be concluded that HASM takes the vegetable type information as global approximate information, and takes local sampling data as its optimum control constraints.

Single rice growth period was prolonged by cultivars shifts, but yield was damaged by climate change during 1981-2009 in China, and late rice was just opposite.

Based on the crop trial data during 1981-2009 at 57 agricultural experimental stations across the North Eastern China Plain (NECP) and the middle and lower reaches of Yangtze River (MLRYR), we investigated how major climate variables had changed and how the climate change had affected crop growth and yield in a setting in which agronomic management practices were taken based on actual weather. We found a significant warming trend during rice growing season, and a general decreasing trend in solar radiation (SRD) in the MLRYR during 1981-2009. Rice transplanting, heading, and maturity dates were generally advanced, but the heading and maturity dates of single rice in the MLRYR (YZ_SR) and NECP (NE_SR) were delayed. Climate warming had a negative impact on growth period lengths at about 80% of the investigated stations. Nevertheless, the actual growth period lengths of YZ_SR and NE_SR, as well as the actual length of reproductive growth period (RGP) of early rice in the MLRYR (YZ_ER), were generally prolonged due to adoption of cultivars with longer growth period to obtain higher yield. In contrast, the actual growth period length of late rice in the MLRYR (YZ_LR) was shortened by both climate warming and adoption of early mature cultivars to prevent cold damage and obtain higher yield. During 1981-2009, climate warming and decrease in SRD changed the yield of YZ_ER by -0.59 to 2.4%; climate warming during RGP increased the yield of YZ_LR by 8.38-9.56%; climate warming and decrease in SRD jointly reduced yield of YZ_SR by 7.14-9.68%; climate warming and increase in SRD jointly increased the yield of NE_SR by 1.01-3.29%. Our study suggests that rice production in China has been affected by climate change, yet at the same time changes in varieties continue to be the major factor driving yield and growing period trends.