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Somatic cell nuclear transfer (SCNT) can be used to reprogram differentiated somatic cells to a totipotent state but has poor efficiency in supporting full-term development. H3K9me3 is considered to be an epigenetic barrier to zygotic genomic activation in 2-cell SCNT embryos. However, the mechanism underlying the failure of H3K9me3 reprogramming during SCNT embryo development remains elusive. Here, we perform genome-wide profiling of H3K9me3 in cumulus cell-derived SCNT embryos. We find redundant H3K9me3 marks are closely related to defective minor zygotic genome activation. Moreover, SCNT blastocysts show severely indistinct lineage-specific H3K9me3 deposition. We identify MAX and MCRS1 as potential H3K9me3-related transcription factors and are essential for early embryogenesis. Overexpression of Max and Mcrs1 significantly benefits SCNT embryo development. Notably, MCRS1 partially rescues lineage-specific H3K9me3 allocation, and further improves the efficiency of full-term development. Importantly, our data confirm the conservation of deficient H3K9me3 differentiation in Sertoli cell-derived SCNT embryos, which may be regulated by alternative mechanisms.
Assuntos
Histonas , Zigoto , Histonas/genética , Técnicas de Transferência Nuclear , Desenvolvimento Embrionário/genética , Blastocisto , Embrião de Mamíferos , Reprogramação Celular/genéticaRESUMO
This paper derives theoretical solutions for three radially layered piezoceramic/epoxy cylindrical composite transducers, which are composed of a solid epoxy disk, two axially polarized piezoceramic rings, and two epoxy rings. Two piezoceramic rings are the functional components, which can actuate and adjust the composite's performance. According to different functions, three typical transducers are developed. The first one involves both of the two piezoceramic rings acting as actuating elements with parallel connections electrically. The other two involve only one piezoceramic ring as an actuating element, while the other ring that is connected to a resistor acts as a sensing element to adjust the electromechanical characteristics. Based on the plane stress assumption, theoretical solutions of these three transducers in radial vibration are derived, and performance differences of their electromechanical characteristics are analyzed and discussed. Furthermore, the solutions are validated by comparing with the ANSYS simulation results and the experimental data. The simulated and the measured first resonance and antiresonance frequencies are in a good agreement with the theoretical results, which validates the accuracy of the directed solution. This paper contributes to a comprehensive understanding of the proposed cylindrical composite's electromechanical performance, which is helpful for further application in underwater sound and ultrasonic fields.
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Dispersion differences and consistency of artificial periodic structures, including phononic crystals, elastic metamaterials, as well as periodic structures composited of phononic crystals and elastic metamaterials, are investigated in this paper. By developing a K(ω) method, complex dispersion relations and group/phase velocity curves of both the single-mechanism periodic structures and the mixing-mechanism periodic structures are calculated at first, from which dispersion differences of artificial periodic structures are discussed. Then, based on a unified formulation, dispersion consistency of artificial periodic structures is investigated. Through a comprehensive comparison study, the correctness for the unified formulation is verified. Mathematical derivations of the unified formulation for different artificial periodic structures are presented. Furthermore, physical meanings of the unified formulation are discussed in the energy-state space.
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This paper deals with a classical problem on a linear elastic lattice. A multi-mass-spring model is proposed to build the unit cell. Based on this multi-mass-spring model, a detailed investigation on the band of frequency gaps of one-dimensional periodic structures is conducted. A unified formulation to study the band structures of one-dimensional periodic structures is obtained. To determine the bound frequencies of the bands of frequency gaps, a very simple method without investigating the dispersion curves is proposed based on the modal analytical method. The method presented in this paper is applicable to general cases and is much more convenient than that proposed by other related investigations. In addition, the dynamic property of a finite periodic structure is investigated from the view of energy input, energy distribution, and interactions between the external excitation and the finite periodic structure, from which the energy flow pattern is illustrated clearly.
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An analytical model of the dynamic characteristics of an axially polarized multilayer piezoelectric/elastic composite cylindrical transducer is proposed in this paper. Based on the plane stress assumption, the dynamic analytical solution of the transducer under an external harmonic voltage load is obtained, and the electric admittance is also derived analytically. Inherent properties of the transducer, such as resonance and anti-resonance frequencies, are presented and discussed. In addition, comparisons with other related investigations are also given, and good agreement is found. The present investigation is very helpful for the design of axially polarized multilayer piezoelectric/elastic composite cylindrical transducers, which can be used in applications related to ultrasonic and underwater sound waves.
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Based on the theory of elasticity and piezoelectricity, a dynamic model of laminated elastic-piezoelectric rectangular plates is considered. The bending equations are established and solved, taking the nonlinear behavior of the piezoelectric material into account. The resonance frequency of a laminated piezoelectric rectangular plate with four kinds of different boundary conditions is then investigated. The present results agree very well with the experimental findings and can be extended to practical applications, such as considering the effect of an epoxy package on the resonance of piezoceramic plate.
RESUMO
Based on Hamilton's principle and the Rayleigh-Ritz method, a model of a nonlinear dynamic laminated piezoelectric rectangular plate was established, and the governing equations were derived and solved for both the thin-plate and thick-plate models. In the present investigation, the nonlinear constitutive relations of piezoelectric materials were considered and the effects of the nonlinearity on the response of the plate were discovered. The primary resonance of rectangular plate is investigated with the use of the method of multiple scales. The results obtained in the present paper agree very well with the experiment results.
