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1.
Phys Rev Lett ; 128(24): 247203, 2022 Jun 17.
Artigo em Inglês | MEDLINE | ID: mdl-35776483

RESUMO

A chiral spin soliton lattice (CSL), one of the representative systems of a magnetic superstructure, exhibits reconfigurability in periodicity over a macroscopic length scale. Such coherent and tunable characteristics of the CSL lead to an emergence of elementary excitation of the CSL as phononlike modes due to translational symmetry breaking and bring a controllability of the dispersion relation of the CSL phonon. Using a broadband microwave spectroscopy technique, we directly found that higher-order magnetic resonance modes appear in the CSL phase of a chiral helimagnet CrNb_{3}S_{6}, which is ascribed to the CSL phonon response. The resonance frequency of the CSL phonon can be tuned between 16 and 40 GHz in the vicinity of the critical field, where the CSL period alters rapidly. The frequency range of the CSL phonon is expected to extend over 100 GHz as extrapolated on the basis of the theoretical model. The present results indicate that chiral helimagnets could work as materials useful for broadband signal processing in the millimeter-wave band.

2.
Tsitol Genet ; 41(3): 13-22, 2007.
Artigo em Inglês | MEDLINE | ID: mdl-17649620

RESUMO

Overwintering crops such as winter wheat display significant increase in freezing tolerance during a period of cold acclimation (CA). To gain better understanding of molecular mechanisms of CA, it is important to unravel functions and regulations of CA-associated genes. Differential screening of a cDNA library constructed from cold acclimated crown tissue of winter wheat identified three novel CA-associated cDNA clones. Nucleotide sequence analysis showed that the clones encode a high mobility globular protein (HMGB1), a glycine-rich RNA-binding protein (TaGRP2), and a LEA D-11 dehydrin (DHN14). Accumulation of the three mRNAs during 14 days of CA was differentially regulated. In response to drought, and ABA, DHN14 mRNA rapidly accumulated while HMGB1 and TaGRP2 mRNA levels remained unchanged. The possible functions of each of these genes in cold acclimation are discussed.


Assuntos
Aclimatação/genética , Temperatura Baixa , Regulação da Expressão Gênica de Plantas , Triticum/genética , Sequência de Bases , Northern Blotting , Clonagem Molecular , DNA Complementar/genética , DNA de Plantas/genética , Genes de Plantas , Dados de Sequência Molecular , Proteínas de Plantas/genética , Alinhamento de Sequência
3.
Mol Genet Genomics ; 275(4): 399-408, 2006 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-16614778

RESUMO

A perennial ryegrass cDNA clone encoding a putative glycine-rich RNA binding protein (LpGRP1) was isolated from a cDNA library constructed from crown tissues of cold-treated plants. The deduced polypeptide sequence consists of 107 amino acids with a single N-terminal RNA recognition motif (RRM) and a single C-terminal glycine-rich domain. The sequence showed extensive homology to glycine-rich RNA binding proteins previously identified in other plant species. LpGRP1-specific genomic DNA sequence was isolated by an inverse PCR amplification. A single intron which shows conserved locations in plant genes was detected between the sequence motifs encoding RNP-1 and RNP-2 consensus protein domains. A significant increase in the mRNA level of LpGRP1 was detected in root, crown and leaf tissues during the treatment of plants at 4 degrees C, through which freezing tolerance is attained. The increase in the mRNA level was prominent at least 2 h after the commencement of the cold treatment, and persisted for at least 1 week. Changes in mRNA level induced by cold treatment were more obvious than those due to treatments with abscisic acid (ABA) and drought. The LpGRP1 protein was found to localise in the nucleus in onion epidermal cells, suggesting that it may be involved in pre-mRNA processing. The LpGRP1 gene locus was mapped to linkage group 2. Possible roles for the LpGRP1 protein in adaptation to cold environments are discussed.


Assuntos
Aclimatação/genética , Lolium/genética , Proteínas de Plantas/genética , Proteínas de Ligação a RNA/genética , Ácido Abscísico/farmacologia , Motivos de Aminoácidos , Sequência de Aminoácidos , Sequência de Bases , Núcleo Celular/metabolismo , Mapeamento Cromossômico , Clonagem Molecular , Temperatura Baixa , Citoplasma/metabolismo , Regulação da Expressão Gênica de Plantas , Ligação Genética , Lolium/efeitos dos fármacos , Dados de Sequência Molecular , Proteínas de Plantas/efeitos dos fármacos , Proteínas de Plantas/metabolismo , Processamento Pós-Transcricional do RNA , Proteínas de Ligação a RNA/efeitos dos fármacos , Proteínas de Ligação a RNA/metabolismo
4.
Plant Cell Rep ; 22(12): 910-8, 2004 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-15042407

RESUMO

We have developed a new procedure for Agrobacterium-mediated transformation of plants in the genus Beta using shoot-base as the material for Agrobacterium infection. The frequency of regeneration from shoot bases was analyzed in seven accessions of sugarbeet ( Beta vulgaris) and two accessions of B. maritima to select materials suitable for obtaining transformed plants. The frequency of transformation of the chosen accessions using Agrobacterium strain LBA4404 and selection on 150-mg/l kanamycin was found to be higher than that in previously published methods. Genomic DNA analysis and beta-glucuronidase reporter assays showed that the transgene was inherited and expressed in subsequent generations. In our method, shoot bases are prepared by a simple procedure, and transformation does not involve the callus phase, thus minimizing the occurrence of somaclonal variations.


Assuntos
Agrobacterium tumefaciens/genética , Beta vulgaris/fisiologia , Chenopodiaceae/fisiologia , Higromicina B/análogos & derivados , Folhas de Planta/fisiologia , Brotos de Planta/fisiologia , Plantas Geneticamente Modificadas/fisiologia , Sequência de Bases , Beta vulgaris/efeitos dos fármacos , Beta vulgaris/genética , Chenopodiaceae/efeitos dos fármacos , Chenopodiaceae/genética , Cinamatos/farmacologia , Primers do DNA , DNA de Plantas/genética , Vetores Genéticos , Higromicina B/farmacologia , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/genética , Brotos de Planta/efeitos dos fármacos , Brotos de Planta/genética , Plantas Geneticamente Modificadas/efeitos dos fármacos , Plasmídeos/genética , Reação em Cadeia da Polimerase , Regeneração , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Transformação Genética
5.
Theor Appl Genet ; 106(3): 445-53, 2003 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-12589544

RESUMO

Soybean was domesticated in East Asia, where various kinds of landraces have been established as a result of adaptation to different environments and the diversification of food cultures. Asia is thus an important germplasm pool of soybean. In order to evaluate the genetic structure of the Asian soybean population, we analyzed allelic profiles at 20 simple-sequence repeat (SSR) loci of 131 accessions introduced from 14 Asian countries. The SSR loci produced an average of 11.9 alleles and a mean gene diversity of 0.782 in the accessions tested. Quantification theory III analysis and cluster analysis with the UPGMA method clearly separated the Japanese from the Chinese accessions, suggesting that the Japanese and Chinese populations formed different germplasm pools. The Korean accessions were involved in both germplasm pools, whereas most of the accessions from southeast and south/central Asia were derived from the Chinese pool. Relatively high genetic diversity and the absence of region-specific clusters in the southeast and south/central Asian populations suggest that soybean in these areas has been introduced repeatedly and independently from the diverse Chinese germplasm pool. The present study indicates that the two germplasm pools can be used as exotic genetic resources to enlarge the genetic bases of the respective Asian soybean populations.


Assuntos
Variação Genética , Glycine max/genética , Repetições de Microssatélites , Sequências Repetitivas de Ácido Nucleico , Alelos , Ásia , Primers do DNA/química , DNA de Plantas , Ecologia , Marcadores Genéticos , Genética Populacional , Filogenia , Reação em Cadeia da Polimerase , Polimorfismo Genético , Glycine max/crescimento & desenvolvimento
6.
Theor Appl Genet ; 105(5): 645-653, 2002 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-12582476

RESUMO

Soybean [ Glycine max (L.) Merr.] is one of the major crops in the world and was domesticated from a wild progenitor, Glycine soja Sieb. & Zucc., in East Asia. In order to address the questions concerning the evolution and maternal lineage of soybean, we surveyed the variation in chloroplast DNA simple sequence repeats (cpSSR) of 326 wild and cultivated soybean accessions that were collected from various Asian countries. Twenty-three variants were detected at six cpSSRs in the accessions tested. All of the variants were found in wild soybean, whereas only 14 variants existed in the cultigen. Combining the variants at the six cpSSRs gave 52 haplotypes in the former and eight haplotypes in the latter. Both analyses indicated a considerably higher genetic diversity in the wild soybean. Around 75% of the cultivated accessions tested possessed a common haplotype (no. 49), which was detected in only seven wild accessions, six from southern Japan and one from southern China. The predominant haplotype in the cultigen may therefore have originated from a rare haplotype of the wild soybean that is presently distributed in the southern areas of Japan and China. The remaining seven haplotypes in the cultigen were distributed regionally, and except for three rare haplotypes, largely overlapped with the distributions of wild accessions with the same respective haplotypes. Our results strongly suggest that the cultivated soybeans with different cpDNA haplotypes originated independently in different regions from different wild gene pools and/or hybrid swarms between cultivated and wild forms.

7.
Anticancer Res ; 21(3B): 1705-12, 2001.
Artigo em Inglês | MEDLINE | ID: mdl-11497250

RESUMO

S-1 is a new oral formulation of 5-fluorouracil (5-FU) containing 1 M tegafur and 0.4 M 5-chloro-2,4-dihydroxypyridine (CDHP) and 1 M potassium oxonate (Oxo). It has been reported to have a high antitumor activity and low gastrointestinal toxicity in rats bearing murine and human tumors. We further studied the possible inhibition of the toxicities caused by the products of 5-FU metabolism with the use of CDHP, a new inhibitor of 5-FU degradation and Oxo, an inhibitor of 5-FU phosphorylation. In a model of pentylenetetrazole-induced convulsions in mice, intravenous injection of fluoroacetate (3 mg/kg), 2-fluoro-b-alanine (30 mg/kg) and 5-FU (over 300 mg/kg) significantly augmented the occurrence of convulsion. However coadministration of an equivalent dose of CDHP with 5-FU almost completely suppressed the 5-FU-augmented convulsions, suggesting that inhibition of 5-FU catabolism by CDHP may lead to a decreased risk of development of 5-FU neurotoxicity. Another advantage of the use of S-1 was protection through Oxo against the development of 5-FU-induced mucositis, which occurs frequently in cancer patients. When 6 mg/kg of S-1 was administered orally to beagle dogs for 5 days, the incidence of stomatitis decreased markedly compared to that in dogs receiving the same dose of S-1 not containing Oxo, in which severe stomatitis was frequently observed. One of the possible mechanisms of the decreased incidence of mucositis associated with oral S-1 administration is the decreased formation of 5-fluoronucleotides from 5-FU in the mucosal tissues of the oral cavity. These results suggest that oral S-1 could be employed for the treatment of cancer patients with marked reduction in the incidence of toxicities including encephalopathy, stomatitis and diarrhea.


Assuntos
Antimetabólitos Antineoplásicos/efeitos adversos , Antimetabólitos Antineoplásicos/farmacologia , Fluoruracila/efeitos adversos , Fluoruracila/farmacologia , Ácido Oxônico/farmacologia , Ácido Oxônico/toxicidade , Piridinas/farmacologia , Piridinas/toxicidade , Tegafur/farmacologia , Tegafur/toxicidade , Animais , Anorexia/induzido quimicamente , Radioisótopos de Carbono/metabolismo , Diarreia/induzido quimicamente , Cães , Combinação de Medicamentos , Fluoracetatos/farmacologia , Fluoruracila/análogos & derivados , Fluoruracila/sangue , Injeções Intravenosas , Masculino , Camundongos , Camundongos Endogâmicos ICR , Mucosa Bucal/efeitos dos fármacos , Mucosa Bucal/metabolismo , Mucosa Bucal/patologia , Pentilenotetrazol/farmacologia , Fatores de Tempo , Vômito/induzido quimicamente
8.
Int J Mol Med ; 8(3): 273-8, 2001 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-11494055

RESUMO

Uridine/cytidine kinase which converts uridine and cytidine to their corresponding monophosphates is a rate-limiting enzyme involved in the salvage pathway of pyrimidine synthesis. We isolated cDNA encoding the enzyme from human fibrosarcoma cells, then determined its nucleotide sequence by the 5'-RACE method followed by confirmation employing the human genome DNA library. The isolated uridine/cytidine kinase cDNA (UCK cDNA) consisted of 786 nucleotides encoding 261 amino acids and was found to have approximately 70% homology with mouse UCK cDNA. Northern blot analysis of human leukemia RNAs with labeled UCK gene showed a single band at 1.6 kb to be UCK mRNA, and southern blot analysis of the UCK cDNA after digestion with BamHI, SacI and XbaI enzymes showed four band signals, suggesting the UCK gene to have at least 4 exons. A truncated form of UCK cDNA was expressed as the His-tag conjugated protein in Escherichia coli. The expressed and purified protein specifically converted uridine and cytidine to their corresponding monophosphates and also phosphorylated antitumor nucleosides such as 5-fluorouridine, cyclopentenyl-cytosine and 3'-C-ethynylcytidine. The present results suggest that our cloned human UCK cDNA encodes the correct amino acid sequence for UCK protein, showing high intracellular phosphorylation activity forward natural and synthetic pyrimidine nucleosides.


Assuntos
Fibrossarcoma/genética , Uridina Quinase/genética , Sequência de Aminoácidos , Sequência de Bases , Northern Blotting , Clonagem Molecular , DNA Complementar/química , DNA Complementar/genética , Feminino , Fibrossarcoma/enzimologia , Fibrossarcoma/patologia , Expressão Gênica , Regulação Neoplásica da Expressão Gênica , Células HL-60 , Células HeLa , Humanos , Células K562 , Dados de Sequência Molecular , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , RNA Neoplásico/genética , RNA Neoplásico/metabolismo , Alinhamento de Sequência , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos , Distribuição Tecidual , Células Tumorais Cultivadas , Uridina Quinase/metabolismo
9.
Genes Genet Syst ; 76(2): 99-105, 2001 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-11434464

RESUMO

beta-conglycinin, a soybean seed storage protein, is comprised of three different subunits, a, alpha', and beta. Several candidates for the alpha subunit gene have been isolated, however, the structure of the alpha subunit gene has not been completely determined. Accordingly, it was also unknown which of the gene candidates are functionally active. Here, we have determined the nucleotide sequence and transcription start site of the alpha subunit gene, and compared the structural components with those of the other subunits or other seed protein genes. The a subunit gene, which is located on a 7.6-kb EcoRI fragment, was composed of six exons that had the same organization as those for the alpha' subunit gene. Within a 400 bp upstream region of the transcription start site, four regions (designated as boxes I, II, III, and IV) were found to be conserved among the alpha, alpha', and other seed protein genes. Genomic Southern blot analysis of soybean varieties lacking the alpha subunit gene candidate indicated that the gene characterized in this paper actually encodes the a subunit and is functionally active. In addition, these experiments revealed the presence of an additional gene which is also responsible for the expression of the a subunit.


Assuntos
Globulinas/genética , Glycine max/genética , Proteínas de Soja , Sequência de Aminoácidos , Antígenos de Plantas , Sequência de Bases , Southern Blotting , Éxons , Íntrons , Modelos Genéticos , Dados de Sequência Molecular , Hibridização de Ácido Nucleico , RNA/metabolismo , RNA Mensageiro/metabolismo , Proteínas de Armazenamento de Sementes , Análise de Sequência de DNA , Homologia de Sequência do Ácido Nucleico , Transcrição Gênica
10.
Hypertens Res ; 24(3): 263-9, 2001 May.
Artigo em Inglês | MEDLINE | ID: mdl-11409649

RESUMO

The present in vitro study was performed to investigate the effects of estriol (E3) on membrane fluidity of erythrocytes by means of an electron paramagnetic resonance (EPR) and spin-labeling method. E3 was shown to significantly decrease the order parameter (S) for 5-nitroxide stearate (5-NS) and the peak height ratio (ho/h-1) for 16-NS obtained from EPR spectra of erythrocyte membranes. This finding indicated that E3 might increase the membrane fluidity of erythrocytes. The effect of E3 was significantly potentiated by the nitric oxide (NO) donor, S-nitroso-N-acetylpenicillamine (SNAP), and a cyclic guanosine 3',5'-monophosphate (cGMP) analog, 8-bromo-cGMP. In contrast, the change in the membrane fluidity induced by E3 was antagonized by the NO synthase inhibitor, L-NG-nitroarginine-methyl-ester (L-NAME), and asymmetric dimethyl-L-arginine (ADMA). The results of the present study showed that E3 significantly increased the membrane fluidity and improved the microviscosity of erythrocyte membranes, partially mediated by an NO- and cGMP-dependent pathway. Furthermore, the data might be consistent with the hypothesis that E3 could have a beneficial effect on the rheological behavior of erythrocytes and may play a crucial role in the regulation of microcirculation.


Assuntos
Arginina/análogos & derivados , GMP Cíclico/análogos & derivados , Membrana Eritrocítica/efeitos dos fármacos , Membrana Eritrocítica/metabolismo , Estriol/farmacologia , Fluidez de Membrana/efeitos dos fármacos , Óxido Nítrico/metabolismo , Penicilamina/análogos & derivados , Arginina/farmacologia , GMP Cíclico/farmacologia , Espectroscopia de Ressonância de Spin Eletrônica , Inibidores Enzimáticos/farmacologia , Feminino , Humanos , Técnicas In Vitro , Masculino , Pessoa de Meia-Idade , NG-Nitroarginina Metil Éster/farmacologia , Doadores de Óxido Nítrico/farmacologia , Penicilamina/farmacologia
11.
Jpn J Cancer Res ; 92(3): 343-51, 2001 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-11267946

RESUMO

We examined the effects of dosage schedule on antitumor activity in vitro and in vivo to determine the optimal administration schedule for a new nucleoside antimetabolite 1-(3-C-ethynyl-beta-D-ribo-pentofuranosyl)cytosine (ECyd, TAS-106). The cytotoxicity of TAS-106 in vitro against human tumors was evaluated at three drug exposure periods. TAS-106 exhibited fairly potent cytotoxicity even with 4 h exposure, and nearly equivalent and sufficiently potent cytotoxicity with 24 and 72 h exposures. These results suggest that long-term exposure to TAS-106 will not be required to achieve maximal cytotoxicity. The antitumor activity of TAS-106 in vivo was compared in nude rat models bearing human tumors on three administration schedules, once weekly, 3 times weekly, and 5 times weekly for 2 or 4 consecutive weeks. TAS-106 showed strong antitumor activity without serious toxicity on all three schedules, but the antitumor activity showed no obvious schedule-dependency in these models. When tumor-bearing nude rats were given a single i.v. dose of [(3)H]TAS-106, tumor tissue radioactivity tended to remain high for longer periods of time as compared to the radioactivity in various normal tissues. Furthermore, when the metabolism of TAS-106 in the tumor was examined, it was found that TAS-106 nucleotides (including the active metabolite, the triphosphate of TAS-106) were retained at high concentrations for prolonged periods. These pharmacodynamic features of TAS-106 may explain the strong antitumor activity without serious toxicity, observed on intermittent administration schedules, in nude rat models with human tumors. We therefore consider TAS-106 to be a promising compound which merits further investigation in patients with solid tumors.


Assuntos
Antimetabólitos Antineoplásicos/farmacocinética , Antimetabólitos Antineoplásicos/uso terapêutico , Sobrevivência Celular/efeitos dos fármacos , Citidina/farmacocinética , Citidina/uso terapêutico , Neoplasias Pulmonares/tratamento farmacológico , Neoplasias Gástricas/tratamento farmacológico , Animais , Antimetabólitos Antineoplásicos/toxicidade , Neoplasias da Mama , Neoplasias do Colo , Citidina/análogos & derivados , Citidina/toxicidade , Feminino , Humanos , Masculino , Neoplasias Pancreáticas , Ratos , Ratos Endogâmicos F344 , Ratos Nus , Distribuição Tecidual , Transplante Heterólogo , Células Tumorais Cultivadas
12.
J Cardiovasc Pharmacol ; 38 Suppl 1: S75-9, 2001 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-11811365

RESUMO

To evaluate the mechanism of neurally mediated syncope (NMS), we investigated basal autonomic nerve function using a conventional pharmacological method and [123I]-metaiodobenzyl-guanidine (MIBG) single photon emission computed tomography (SPECT). Nine patients with NMS, whose syncope was induced by head-up tilt test with or without isoproterenol, underwent [123I]-MIBG SPECT. Eight of nine NMS patients showed reduced myocardial uptake (two patients, diffuse; four patients, anteroseptal and inferior; one patient, anteroseptal; one patient, inferior). In the study of pharmacological autonomic nervous function test, atropine sulfate (atr.) (0.04 mg/kg), isoproterenol (isp.) (5 x 10(-3) microg/kg/min), propranolol (prop.) (0.2 mg/kg), phenylephrine (phenyl.) (0.4 microg/kg/min), and phentolamine (phent.) (0.2 mg/kg) were successively administered to patients with NMS (n = 5) and control subjects (n = 5). The heart rate (HR) after atr. and prop., and systolic blood pressure (SBP) after phent. were defined as intrinsic HR (IHR) and intrinsic SBP (ISBP). Parasympathetic activity (increase in HR by atr.), beta-sympathetic tone (HR after atr. minus IHR), beta-sensitivity (change in HR by 1 microg isp./kg/min), beta-secretion (beta-tone/beta-sensitivity), alpha-sympathetic tone (SBP before phenyl. minus ISBP), alpha-sensitivity (change in SBP by 1 microg phenyl./kg/min) and alpha-secretion (alpha-tone/alpha-sensitivity) were also calculated. The beta-secretion was decreased (0.0027+/-0.0008 versus 0.0060+/-0.0004 microg/kg/min/isp.; p < 0.05), while the beta-sensitivity was increased (5850+/-947 versus 3150+/-292 beats/microg/kg/min isp.; p < 0.05) in NMS compared with control subjects. In the other indexes, there were no significant differences between two groups. The results of the present study suggest that increased beta-sensitivity may contribute hypercontraction of left ventricles, which might partially explain the mechanism of NMS.


Assuntos
Receptores Adrenérgicos beta/fisiologia , Síncope Vasovagal/diagnóstico , Síncope Vasovagal/fisiopatologia , 3-Iodobenzilguanidina , Adolescente , Adrenérgicos/farmacologia , Fibras Adrenérgicas/efeitos dos fármacos , Fibras Adrenérgicas/fisiologia , Adulto , Sistema Nervoso Autônomo/efeitos dos fármacos , Sistema Nervoso Autônomo/fisiologia , Feminino , Testes de Função Cardíaca/efeitos dos fármacos , Testes de Função Cardíaca/métodos , Testes de Função Cardíaca/estatística & dados numéricos , Humanos , Masculino , Pessoa de Meia-Idade , Estatísticas não Paramétricas , Tomografia Computadorizada de Emissão de Fóton Único
13.
Ann Hematol ; 79(7): 378-88, 2000 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-10965786

RESUMO

Seven patients with peripheral B-cell lymphoma associated with hemophagocytic syndrome are reported. In all cases, the histologic subtype was diffuse large B-cell lymphoma. Hemophagocytic features were noted in the bone marrow with lymphomatous infiltration. Hemophagocytic syndrome occurred with presentation of the lymphoma and was characterized by high fever, cytopenias, and elevated levels of lactate dehydrogenase, ferritin, C-reactive protein, and cytokines [interferon gamma, macrophage colony-stimulating factor, soluble interleukin (sIL)-2R, and IL-6] without evidence of infection. The phenotypes of lymphomas were suspected CD19+, CD20+, S-Ig+, CD10-, and coexpression of CD5 in some cases. Flow cytometric analysis showed a low CD4/CD8 ratio in peripheral blood and bone marrow. We suggest that the pathogenesis of hemophagocytic syndrome is hypercytokinemia induced by a proliferation of reactive CD8+ T cells. Previous reports of B-cell lymphoma with hemophagocytic syndrome demonstrated similar clinical manifestations and poor prognoses. The invasion patterns of these diffuse large B-cell lymphomas with hemophagocytosis may be classified into three groups: microscopic lymph-node involvement type, gross lymph-node involvement type, and splenic lymphoma type. Although hemophagocytic syndromes have been reported to be associated with T-cell lymphomas, our results indicate an association with diffuse large B-cell lymphoma.


Assuntos
Histiocitose de Células não Langerhans/complicações , Linfoma de Células B/complicações , Idoso , Biópsia , Células Sanguíneas/citologia , Células da Medula Óssea/citologia , Relação CD4-CD8 , Feminino , Rearranjo Gênico , Hemoglobinas/metabolismo , Histiocitose de Células não Langerhans/tratamento farmacológico , Humanos , Interferon gama/sangue , Interleucina-6/sangue , Cariotipagem , L-Lactato Desidrogenase/sangue , Linfoma de Células B/diagnóstico , Linfoma de Células B/patologia , Linfoma não Hodgkin/complicações , Fator Estimulador de Colônias de Macrófagos/sangue , Masculino , Pessoa de Meia-Idade , Contagem de Plaquetas , Receptores de Interleucina-2/sangue , Subpopulações de Linfócitos T
14.
Hereditas ; 132(1): 43-8, 2000.
Artigo em Inglês | MEDLINE | ID: mdl-10857258

RESUMO

A physical map of the azuki bean (Vigna angularis cv. Erimo-shozu) chloroplast DNA (cpDNA) was constructed by localising the cleavage sites of PstI, SalI, SmaI, SacI, KpnI, PvuII and XhoI. The resulting map is more similar to the cpDNA-maps of two Vigna species (mung bean, V. radiata, and V. nakashimae) than to common bean (Phaseolus vulgaris) cpDNA map. Azuki bean was originally classified in the genus Phaseolus, and the inclusion of this taxon in the genus Vigna is a recent taxonomic decision. Our result is thus in favour of the taxonomic placement of azuki bean in the same genus as V. nakashimae and mung bean. We also found that a weed-form accession of azuki bean has a 96-bp deletion relative to the cultivar Erimo-shozu. The 96-bp deletion is located between the trnS-UGA and psbC genes in the large single-copy region of the chloroplast genome. This deletion is flanked by imperfect 9-bp direct repeats, suggesting that the deletion was a result of intra-molecular recombination mediated by these direct repeats.


Assuntos
Cloroplastos/genética , Fabaceae/genética , Deleção de Genes , Genoma de Planta , Plantas Medicinais , Sequência de Bases , Modelos Genéticos , Dados de Sequência Molecular , Mapeamento Físico do Cromossomo , Mapeamento por Restrição , Homologia de Sequência do Ácido Nucleico
15.
Intern Med ; 39(2): 182-4, 2000 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-10732842

RESUMO

A 22-year-old Japanese man developed polyarthritis with fever and urethritis. He was diagnosed as Reiter's syndrome since he was found to have uveitis and persistent aseptic pyuria. Although, he was negative for HLA-B27 or any other HLA-B27 cross-reactive MHC class I antigens, he was positive for HLA-B51. The laboratory examination showed significant elevation of serum IgG and IgA anti-Chlamydia antibodies. He was successfully treated with a combination of doxycycline, naproxen, salazosulfapyridine and methotrexate with a decrease in IgG and IgA anti-Chlamydia antibodies. Previous studies provided evidence that HLA-B51 itself might be involved in the development of Behcet's disease, which shares common features with Reiter's syndrome, such as uveitis, skin lesions, and polyarthritis. It is therefore suggested that combination of Chlamydia infection and HLA-B51 might play a role in the pathogenesis of Reiter's syndrome in our patient.


Assuntos
Artrite Reativa/imunologia , Antígenos HLA-B/imunologia , Adulto , Anticorpos Antibacterianos/análise , Artrite Reativa/sangue , Artrite Reativa/etiologia , Biomarcadores/sangue , Chlamydia/imunologia , Infecções por Chlamydia/sangue , Infecções por Chlamydia/complicações , Infecções por Chlamydia/imunologia , Antígeno HLA-B27/imunologia , Antígeno HLA-B51 , Humanos , Imunoglobulina A/sangue , Imunoglobulina G/sangue , Masculino , Síndrome
16.
Nucleic Acids Symp Ser ; (44): 225-6, 2000.
Artigo em Inglês | MEDLINE | ID: mdl-12903350

RESUMO

In this study, we have investigated non-enzymatic oligomerization of an activated racemic mononucleotide in the presence of Na(+)-montmorillonite. Oligomers up to the decamer in length were formed by oligomerization reactions of activated D- and L-mononucleotides. Similarly, oligomerization of an activated racemic mononucleotide results in the formation of oligomers up to the octamer. These results suggest that montmorillonite catalysis is quite efficient for the oligomerization of racemic monomers, though it is somewhat less efficient than that of D- and L-monomers.


Assuntos
Monofosfato de Adenosina/análogos & derivados , Monofosfato de Adenosina/química , Oligorribonucleotídeos/síntese química , Bentonita , Catálise , Cromatografia Líquida de Alta Pressão , Oligorribonucleotídeos/química , Oligorribonucleotídeos/isolamento & purificação , Estereoisomerismo
17.
J Infect Chemother ; 6(1): 61-2, 2000 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-11810533

RESUMO

A-75-year old woman with agammaglobulinemia developed Moraxella catarrhalis bacteremic pneumonia. M. catarrhalis pneumonia is rarely associated with bacteremia, and neutrophils have been reported as a significant factor in the host defense system against this bacteria. This case suggests that immunoglobulin also plays a key role in the host defense system against M. catarrhalis.


Assuntos
Agamaglobulinemia/complicações , Bacteriemia/microbiologia , Hospedeiro Imunocomprometido/imunologia , Moraxella catarrhalis/isolamento & purificação , Infecções por Neisseriaceae/microbiologia , Pneumonia Bacteriana/microbiologia , Idoso , Bacteriemia/imunologia , Feminino , Humanos , Infecções por Neisseriaceae/imunologia , Pneumonia Bacteriana/imunologia , Fatores de Risco
18.
J Smooth Muscle Res ; 35(3): 87-98, 1999 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-10593574

RESUMO

The roles of intracellular Ca2+ store and protein kinase C (PKC) in vascular contractile responses independent of Ca2+ influx were studied using aortic rings from spontaneously hypertensive rat (SHR) and Wistar-Kyoto rat (WKY). The functional sizes of agonist-sensitive intracellular Ca2+ store were estimated as the peak response to agonist after PKC inhibition with calphostin C (Cal-C), a PKC inhibitor. The participation of PKC in 5-hydroxytryptamine-, phenylephrine-, and endothelin-1 (ET-1)-induced contractions in aortae of SHR was equal to, or greater than that in WKY. In contrast, compared with WKY, SHR aortae possessed a greater size of endothelin-1-sensitive Ca2+ store, a similar size of 5-hydroxytryptamine-sensitive Ca2+ store, and a smaller size of phenylephrine sensitive Ca2+ store. Based on these data, both PKC activation and functional size of intracellular Ca2+ store differ between SHR and WKY and these differences are selective among agosists.


Assuntos
Aorta/enzimologia , Cálcio/metabolismo , Contração Muscular/fisiologia , Músculo Liso Vascular/enzimologia , Proteína Quinase C/metabolismo , Animais , Cálcio/farmacologia , Carcinógenos/farmacologia , Endotelina-1/farmacologia , Inibidores Enzimáticos/farmacologia , Indóis/farmacologia , Masculino , Contração Muscular/efeitos dos fármacos , Naftalenos/farmacologia , Fenilefrina/farmacologia , Proteína Quinase C/antagonistas & inibidores , Ratos , Ratos Endogâmicos SHR , Ratos Endogâmicos WKY , Sarcolema/enzimologia , Serotonina/farmacologia , Acetato de Tetradecanoilforbol/farmacologia , Vasoconstritores/farmacologia
19.
J Biol Chem ; 274(49): 34605-12, 1999 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-10574924

RESUMO

We previously identified various upstream and downstream regulatory elements and factors important for hepatic expression of the human angiotensinogen (ANG) gene, the precursor of vasoactive octapeptide angiotensin II. In the present study, to further investigate the molecular mechanism of human ANG transcriptional regulation, we generated transgenic mice carrying the fusion gene composed of the 1. 3-kilobase promoter of the human ANG gene, its downstream enhancer, and the chloramphenicol acetyltransferase reporter gene. Because expression of the chloramphenicol acetyltransferase gene was observed strongly in the liver and weakly in the kidney, we suspected that hepatocyte nuclear factor (HNF) 4 with a tissue expression pattern similar to that of the reporter gene would regulate ANG transcription. In vitro assays indicated that HNF4 bound to the promoter elements and strongly activated the ANG transcription, but that chicken ovalbumin upstream promoter transcription factor (COUP-TF), a transcriptional repressor, dramatically repressed human ANG transcription through the promoter elements and the downstream enhancer core elements. Furthermore, COUP-TF dramatically decreased the human ANG transcription in the mouse liver by the Helios Gene Gun system in vivo. These results suggest that an interplay between HNF4 and COUP-TF could be important in hepatic human ANG transcription.


Assuntos
Angiotensinas/genética , Proteínas de Ligação a DNA/genética , Proteínas de Ligação a DNA/metabolismo , Regulação da Expressão Gênica , Fosfoproteínas/metabolismo , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , Angiotensinas/biossíntese , Animais , Sequência de Bases , Fatores de Transcrição de Zíper de Leucina e Hélice-Alça-Hélix Básicos , Sítios de Ligação , Fator I de Transcrição COUP , Núcleo Celular/metabolismo , Cloranfenicol O-Acetiltransferase/metabolismo , Relação Dose-Resposta a Droga , Deleção de Genes , Fator 4 Nuclear de Hepatócito , Humanos , Fígado/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos ICR , Camundongos Transgênicos , Dados de Sequência Molecular , Ligação Proteica , Elementos de Resposta , Transcrição Gênica , Células Tumorais Cultivadas
20.
J Vet Med Sci ; 61(6): 609-13, 1999 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-10423681

RESUMO

We previously reported (Arch. Toxcol. 1998, 72, 492-498) that the differential decrease in the levels of hepatic cytochrome P450 (CYP) isozymes in rats was observed 24 hr after intracerebroventricular (i.c.v.) injection of bacterial lipopolysaccharide (LPS) at the dose ineffective (0.1 microgram) when injected intraperitoneally (i.p.). Among CYP isozymes we examined, the male specific CYP isozyme, CYP2C11 was most severely affected by i.c.v. injection of LPS. In this study, we examined the gene expression of CYP2C11, the total P450 contents, the CYP2C11-dependent activity of imipramine N-demethylase (IMND) and protein of CYP2C11 10 hr after i.c.v. or i.p. injections of LPS. Intracerebroventricular injection of LPS significantly decreased the level of CYP2C11 mRNA (to 63% of saline i.c.v. control), the total P450 contents (to 70% of saline i.c.v. control), the IMND activity (to 74% of saline i.c.v. control), but not protein of CYP2C11 in rat liver. In contrast, i.p. injection of LPS at the same dose as i.c.v. did not significantly affect these parameters. Since CYP is a heme protein, we also measured the activity of heme oxygenase (HO) using the same rat liver microsomes. The HO activity was increased to 166% by i.c.v. injection of LPS and 135% by i.p. injection of LPS compared to corresponding saline control. It is suggested that i.c.v. injection of LPS down-regulates the expression of CYP2C11 at transcriptional level and that both the decrease in CYP2C11 mRNA and the increase in heme degradation may be involved in the decreased level of protein and activity of CYP2C11 by i.c.v. injection of LPS in rat liver.


Assuntos
Hidrocarboneto de Aril Hidroxilases , Sistema Enzimático do Citocromo P-450/metabolismo , Heme Oxigenase (Desciclizante)/metabolismo , Lipopolissacarídeos/farmacologia , Microssomos Hepáticos/enzimologia , Esteroide 16-alfa-Hidroxilase , Esteroide Hidroxilases/metabolismo , Inibidores da Captação Adrenérgica/metabolismo , Animais , Northern Blotting , Sistema Enzimático do Citocromo P-450/genética , Família 2 do Citocromo P450 , Regulação para Baixo/efeitos dos fármacos , Escherichia coli , Expressão Gênica/efeitos dos fármacos , Imipramina/metabolismo , Injeções Intraperitoneais , Injeções Intraventriculares , Isoenzimas/metabolismo , Lipopolissacarídeos/administração & dosagem , Masculino , Microssomos Hepáticos/metabolismo , RNA Mensageiro/genética , Ratos , Ratos Wistar , Esteroide Hidroxilases/genética
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