Electrical Stimulation Intensity to Induce Sensory Reweighting Dynamics While Standing on Balance Board.

In human postural control, maladaptation of sensory reweighting to sudden environmental changes is one of the main causes of postural instability. Providing sensory cues for body motion by means of stimulation could induce the sensory reweighting dynamics. In this paper, we aimed to investigate the intensity level of electrical stimulation to induce sensory reweighting dynamics while standing on a balance board under three conditions: no stimulation (control), electrotactile stimulation (ETS) at a low-intensity level, and electrical muscle stimulation (EMS) at a high-intensity level. A total of 30 participants (ten for each condition) controlled their posture to keep the board horizontal in a balance-board task, which included a pre-test without stimulation, a stimulation test, and a post-test without stimulation. The EMS and ETS groups received electrical stimulation to the tibialis anterior or soleus muscles based on the board tilt. Before and after the balance-board task, participants performed static standing with their eyes open and also with their eyes closed to evaluate the visual reweighting. In the EMS group, the visual reweighting showed a strong negative correlation with the balance-board sway ratio between the pre- and stimulation tests, indicating that EMS induced a tendency that requires visual up-weighting to improve postural balance. However, there were no significant correlations between either parameter in the control and ETS groups. These results suggest that high-intensity electrical stimulation at the level of directly contracting muscles may be effective in reliably inducing sensory reweighting dynamics, while low-intensity electrical stimulation may be insufficient.Clinical relevance- These findings will be helpful for designing stimulus conditions to reliably induce the reweighting during balance training, and for establishing a new balance training method utilizing EMS to induce visual up-weighting.

Induced effects of electrical muscle stimulation and visual stimulation on visual sensory reweighting dynamics during standing on a balance board.

Providing instruction cues on body motions using stimulations has the potential to induce sensory reweighting dynamics. However, there are currently very few quantitative investigations on the difference in the induced effects on the sensory reweighting dynamics between stimulation methods. We therefore investigated the difference in the induced effects of electrical muscle stimulation (EMS) and visual sensory augmentation (visual SA) on sensory reweighting dynamics during standing on a balance board. Twenty healthy participants controlled their posture to maintain the board horizontally in the balance-board task, which included a pre-test without stimulation, a stimulation test, and a post-test without stimulation. The EMS group (n = 10) received EMS to the tibialis anterior or soleus muscle based on the board tilt. The visual SA group (n = 10) received visual stimuli via a front monitor based on the board tilt. We measured the height of the board marker and calculated the board sway. Before and after the balance-board task, the participants performed static standing with their eyes open and closed. We measured postural sway and calculated the visual reweighting. The visual reweighting showed a strong negative correlation with the balance board sway ratio between the pre- and stimulation tests in the EMS group and a strong positive correlation with that in the visual SA group. Moreover, for those who reduced the balance board sway in the stimulation test, the visual reweighting was significantly different between the stimulation methods, demonstrating that the induced effect on sensory reweighting dynamics is quantitatively different depending on which method is used. Our findings suggest that there is an appropriate stimulation method to change to the targeted sensory weights. Future investigations on the relationship between sensory reweighting dynamics and stimulation methods could contribute to the proposal and implementation of new training methods for learning to control the target weights.

Force Control on Fingertip Using EMS to Maintain Light Touch.

Light touch on a rigid surface with minimal force below a specific threshold reduces postural sway by providing additional sensory cues from the fingertips. The feasibility of maintaining light touch depends on subject characteristics and task difficulty. Therefore, we introduce a method of maintaining light touch by using electrical muscle stimulation (EMS). We applied it in a single-leg standing task involving healthy adult subjects. The subjects stood upright in a single-leg stance on a firm surface and on foam rubber (FR), respectively, under three conditions: no touch (NT, NT-FR), light touch without EMS (LT, LT-FR), and light touch in which EMS was applied based on the contact force (LT-EMS, LT-EMS-FR). The results showed that the force control by EMS helped maintain light touch and reduce postural sway compared with the no-touch condition. The amplitude of postural sway under the touch condition with EMS was equivalent to that under the touch condition without EMS.

Shoot has important roles in strigolactone production of rice roots under sulfur deficiency.

Strigolactones (SLs) are a class of plant hormones that control plant architecture. SL levels in roots are determined by the nutrient conditions in the rhizosphere, especially the levels of nitrogen (N) and phosphorus (P). Our previous research showed that SL production is induced in response to deficiency of sulfur (S) as well as of N and P, and inhibits shoot branching, accelerates leaf senescence, and regulates lamina joint angle in rice. Here we show biomass, total S contents, and SL levels in rice under S-sufficient and S-deficient conditions using a split-root system. When one part of the root system was cultured in S-sufficient medium and the other in S-deficient medium (+S/-S), shoot fresh weight was unaffected relative to the +S/+S condition. The shoot weight significantly decreased in -S/-S condition. In contrast, there was no significant difference in root fresh weight between +S and -S conditions. In +S/-S condition, SL levels were systemically reduced in both parts, the shoot S content increased, but the root S content in S-deficient medium was unaffected relative to the -S/-S condition. These results suggest that shoots, not roots, recognize S deficiency, which induces SL production in roots.

Strigolactones Decrease Leaf Angle in Response to Nutrient Deficiencies in Rice.

Strigolactones (SLs) are a class of plant hormones that are synthesized from ß-carotene through sequential reactions catalyzed by DWARF (D) 27, D17, D10, and OsMORE AXILLARY GROWTH (MAX) 1 in rice (Oryza sativa L.). In rice, endogenous SL levels increase in response to deficiency of nitrogen, phosphate, or sulfate (-N, -P, or -S). Rice SL mutants show increased lamina joint (LJ) angle as well as dwarfism, delayed leaf senescence, and enhanced shoot branching. The LJ angle is an important trait that determines plant architecture. To evaluate the effect of endogenous SLs on LJ angle in rice, we measured LJ angle and analyzed the expression of SL-biosynthesis genes under macronutrient deficiencies. In the "Shiokari" background, LJ angle was significantly larger in SL mutants than in the wild-type (WT). In WT and SL-biosynthesis mutants, direct treatment with the SL synthetic analog GR24 decreased the LJ angle. In WT, deficiency of N, P, or S, but not of K, Ca, Mg, or Fe decreased LJ angle. In SL mutants, deficiency of N, P, or S had no such effect. We analyzed the time course of SL-related gene expression in the LJ of WT deficient in N, P, or S, and found that expression of SL-biosynthesis genes increased 2 or 3 days after the onset of deficiency. Expression levels of both the SL-biosynthesis and signaling genes was particularly strongly increased under -P. Rice cultivars "Nipponbare", "Norin 8", and "Kasalath" had larger LJ angle than "Shiokari", interestingly with no significant differences between WT and SL mutants. In "Nipponbare", endogenous SL levels increased and the LJ angle was decreased under -N and -P. These results indicate that SL levels increased in response to nutrient deficiencies, and that elevated endogenous SLs might negatively regulate leaf angle in rice.

Low Infection of Phelipanche aegyptiaca in Micro-Tom Mutants Deficient in CAROTENOIDCLEAVAGE DIOXYGENASE 8.

Strigolactones (SLs), a group of plant hormones, induce germination of root-parasitic plants and inhibit shoot branching in many plants. Shoot branching is an important trait that affects the number and quality of flowers and fruits. Root-parasitic plants, such as Phelipanche spp., infect tomato roots and cause economic damage in Europe and North Africa-hence why resistant tomato cultivars are needed. In this study, we found carotenoid cleavage dioxygenase 8-defective mutants of Micro-Tom tomato (slccd8) by the "targeting induced local lesions in genomes" (TILLING) method. The mutants showed excess branching, which was suppressed by exogenously applied SL. Grafting shoot scions of the slccd8 mutants onto wild-type (WT) rootstocks restored normal branching in the scions. The levels of endogenous orobanchol and solanacol in WT were enough detectable, whereas that in the slccd8 mutants were below the detection limit of quantification analysis. Accordingly, root exudates of the slccd8 mutants hardly stimulated seed germination of root parasitic plants. In addition, SL deficiency did not critically affect the fruit traits of Micro-Tom. Using a rhizotron system, we also found that Phelipanche aegyptiaca infection was lower in the slccd8 mutants than in wild-type Micro-Tom because of the low germination. We propose that the slccd8 mutants might be useful as new tomato lines resistant to P. aegyptiaca.

Upregulation of DWARF27 is associated with increased strigolactone levels under sulfur deficiency in rice.

Plants produce strigolactones (SLs) in roots in response to nitrogen or phosphate deficiency. To evaluate SL levels under other mineral deficiencies in rice, we cultivated rice seedlings in hydroponic media without nitrogen, phosphorus, potassium, sulfur, calcium, magnesium, and iron. Tiller bud outgrowth was stimulated under calcium deficiency because of low SL levels. SL levels increased under sulfur deficiency, in addition to phosphate, and nitrogen deficiencies. To explore which genes are key regulators of SL production under sulfur deficiency, we analyzed the expression of SL-related genes in sulfur-sufficient and sulfur-deficient conditions. An SL biosynthesis gene, DWARF27 (D27), was strongly expressed under sulfur deficiency, and its expression was decreased by sulfur supply. The levels of D10, D17, and OsMAX1 transcripts did not differ between sulfur-sufficient and sulfur-deficient conditions. These results suggest that the increased SL levels under sulfur deficiency are due to a high expression of D27. A combination of nitrogen, phosphorus, and sulfur deficiencies had no additive synergistic effect on SL production. Under combined phosphorus and sulfur deficiency, the expression levels of most SL biosynthesis genes were elevated. The number of tiller buds in the d27 mutant was higher than in the wild type, but lower than in other d mutants. Under sulfur deficiency, the chlorophyll content of d27 was lower than those of other d mutants. These results indicate that D27 plays an important role in adaptation to sulfur deficiency in rice.