DSLET and ACTH(4-10) increase mitotic activity of hepatocytes and suppress antibody production.

The mitotic index of hepatocytes remained unchanged after 10 intraperitoneal injections of DSLET and ACTH(4-10) in doses of 0.5, 1.5, and 5 microg/kg, but increased after injection of these substances in doses of 50 and 150 microg/kg. DSLET in doses of 5, 50, and 150 microg/kg decreased the number of antibody-producing cells in the spleen. ACTH(4-10) possessed immunosuppressive activity not only in these doses, but also in a dose of 1.5 microg/kg. As differentiated from mitotic activity of hepatocytes, the degree of immunosuppression increased with increasing the dose of test peptides.

[Pharmacological correction of immuno-metabolic disorders with the peptide Gly-His-Lys in hepatic damage induced by tetrachloromethane]. / Farmakologicheskaia korrektsiia peptidom Gly-His-Lys immunometabolicheskikh narushenii v usloviiakh porazheniia pecheni tetrakhlormetanom.

10-day intraperitoneal administration of the tripeptie Gly-His-Lys in doses 2.5 and 150 mu/kg raises of mitotic index of hepatocytes in acute toxic damage to the liver. In the dose 1.5 mu/kg this peptie corrects both functional activity of hepatocytes and immunological responsiveness. In a dose of 150 mu/kg the peptie has a more potent immunosuppressive action and deteriorates biochemical indices of blood serum as well as dystrophic changes in the liver.

Tripeptide Gly-His-Lys is a hepatotropic immunosuppressor.

Ten intraperitoneal injections of tripeptide Gly-His-Lys in doses of 1.5, 5, 50, 150, and 450 mg/kg stimulated mitotic activity of hepatocytes and dose-dependently suppressed immune reactivity (number of antibody-producing cells and delayed-type hypersensitivity reaction).

[Effect of humoral factors of the spleen of rats with acute and chronic toxic liver involvement on the physiologic and reparative regeneration of hepatocytes]. / Vliianie gumoral'nykh faktorov selezenki krys s ostrym i khronicheskim toksicheskim porazheniem pecheni na fiziologicheskuiu i reparativnuiu regeneratsiiu gepatotsitov.

It was demonstrated in experiments on Wistar rats with carbon tetrachloride induced acute and chronic affection of the liver that the supernatant of the splenic cells of the poisoned animals contains 2 undialyzable factors with a molecular mass of more than 150 and 10-12 kDa. The splenocytic substances stimulate hepatocyte proliferation both in healthy allogeneic recipients and under conditions of increased proliferative activity of hepatocytes in experimental pathology of the liver.

[Effect of free and immobilized terrilytin on the proliferative activity of normal hepatocytes and under physical loading and exposure to vibration]. / Vliianie svobodnogo i immobilizovannogo terrilitina na proliferativnuiu aktivnost' gepatotsitov v norme, pri fizicheskoi nagruzke i vibratsionnom vozdeistvii.

Administration of free and to a greater extent immobilized terrilytin to intact animals significantly enhanced the proliferative activity of hepatocytes and cells of the eye cornea under normal conditions, during physical loading and vibration. At administration of preparations of proteases splenocytes release two protein functionally different substances--a low molecular one exerting the immunostimulating effect and a relatively high molecular one enhancing the proliferative activity of nonimmunocompetent cells (hepatocytes and epitheliocytes of the cornea). The results of the studies indicate the presence of a close relationship between the immunological and nonimmunological effects caused by proteolytic enzymes.

[Role of macrophages in the immune response to soluble protein antigen and in staphylococcal infection]. / Rol' makrofagov pri immunnom otvete na belkovyi rastvorimyi antigen i pri stafilokokkovoi infektsii.

In experiments on rabbits immunized with soluble protein antigen immune reactions were found to be accompanied by the production of lipofuscin in macrophages. This process was the morphological manifestation of the digestion of antigen by macrophages which thus acquired the ability to migrate in the organ and to form lymphoid follicules in the medullary zone of lymph nodes. The newly formed follicules seem to be the basis of pronounced specific immune response. In staphylococcal bacteriemia the phagocytic activity of macrophages was delayed, thus causing disturbances in lipofuscin production; as a result, the subsequent phases of immune response also lagged somewhat behind in time.