RESUMO
Reconditioning of food processing water streams for reuse is an increasingly common water management practice in the food industry and UV disinfection is often employed as part of the water treatment. Several factors may impact the effect of UV radiation. Here, we aim to assess the impact of cell aggregation on UV inactivation kinetics and investigate if UV exposure induces aggregation. Three strains, isolated from food processing water reuse lines (Raoultella ornithinolytica, Pseudomonas brenneri, Rothia mucilaginosa) and both an aggregating and a non-aggregating strain of Staphylococcus aureus were exposed to UVC light at 255 nm using UV LED equipment. Total Viable Count and phase-contrast microscopy, coupled with image analysis, were used to compare the UV inactivation kinetics with the average particle size for a range of UV doses. Tailing effect, seen as a strong reduction in inactivation rate, was observed for all strains at higher UV doses (industrial strains ≥ 50 or 120 mJ/cm2, S. aureus strains ≥ 40 or 60 mJ/cm2). The naturally aggregating strains were more UV tolerant, both within and between species. When aggregates of S. aureus were broken, UV tolerance decreased. For the processing water isolates, the lowest applied UV dose (25 mJ/cm2) significantly increased the average particle size. Application of higher UV doses obtained with longer exposure times did not further increase the particle size compared with untreated samples. For the S. aureus strains, however, no consistent change in average particle size was observed due to UV. Our results demonstrate that aggregating strains have a higher degree of protection and that UV radiation induces aggregation in some, but not all bacteria. A better understanding of the mechanisms governing microbial aggregation and survival during UV treatment could help to improve UV applications and predictions of microbial inactivation.
Assuntos
Staphylococcus aureus , Raios Ultravioleta , Bactérias , Desinfecção , Enterobacteriaceae , Cinética , Micrococcaceae , PseudomonasRESUMO
The yeast species Saccharomyces cerevisiae and Kluyveromyces marxianus are associated with fermentation of West African indigenous foods. The aim of this study was to characterize potential probiotic properties of S. cerevisiae and K. marxianus isolates from the West African milk products lait caillé and nunu and a cereal-based product mawè. The strains (14 in total) were identified by 26S rRNA gene sequencing and characterized for survival at gastrointestinal stress (bile salts and low pH) and adhesion to Caco-2 intestinal epithelial cells. Selected yeast isolates were tested for their effect on the transepithelial electrical resistance (TEER), using the intestinal epithelial cell line Caco-2 and for maintenance of intracellular pH (pHi ) during perfusion with gastrointestinal pH (3.5 and 6.5). All tested yeasts were able to grow in bile salts in a strain-dependent manner, exhibiting a maximum specific growth rate (µmax ) of 0.58-1.50 h-1 . At pH 2.5, slow growth was observed for the isolates from mawè (µmax of 0.06-0.80 h-1 ), whereas growth of yeasts from other sources was mostly inhibited. Yeast adhesion to Caco-2 cells was strain specific and varied between 8.0% and 36.2%. Selected strains of S. cerevisiae and K. marxianus were able to maintain the pHi homeostasis at gastrointestinal pH and to increase TEER across the Caco-2 monolayers, indicating their potential to improve intestinal barrier functions. Based on overall results, strains of K. marxianus and S. cerevisiae from mawè exhibited the highest probiotic potential and might be recommended for further development as starter cultures in West African fermented products.
Assuntos
Grão Comestível/microbiologia , Fermentação , Alimentos Fermentados/microbiologia , Kluyveromyces/metabolismo , Leite/microbiologia , Probióticos/isolamento & purificação , Saccharomyces cerevisiae/metabolismo , África Ocidental , Animais , Células CACO-2 , Técnicas de Cultura de Células , Meios de Cultura/química , Células Epiteliais/microbiologia , Microbiologia de Alimentos , Humanos , Concentração de Íons de Hidrogênio , Kluyveromyces/genética , Probióticos/análise , Saccharomyces cerevisiae/genéticaRESUMO
Fermented cereal doughs constitute a predominant part of West African diets. The environment of fermented doughs can be hostile for microbial survival due to high levels of microbial metabolites such as weak carboxylic organic acids and ethanol. In order to get a better understanding of the intrinsic factors affecting the microbial successions of yeasts during dough fermentation, survival and physiological responses of the yeasts associated with West African fermented cereal doughs were investigated at exposure to relevant concentrations of microbial inhibitory compounds. Three strains each of the predominant species, i.e. Saccharomyces cerevisiae, Kluyveromyces marxianus, Pichia kudriavzevii as well as the opportunistic pathogen Candida glabrata were studied. The strains were exposed to individual stress factors of cereal doughs, i.e. (i) pHâ¯3.4, (ii) 3% (v/v) ethanol (EtOHpH3.4), (iii) 285â¯mM lactic acid (LApH3.4) and (iv) 150â¯mM acetic acid (AApH3.4) as well as to combinations of these stress factors, i.e. (v) (LAâ¯+â¯AA)pHâ¯3.4 and (vi) (LAâ¯+â¯AA+EtOH)pHâ¯3.4. Growth and single cell viability were studied by flow cytometry using combined SYTO 13 and propidium iodide (PI) staining. Intracellular pH (pHi), plasma membrane integrity and micro-colony development of stressed cells were studied by fluorescence microscopy using PI and carboxyfluorescein diacetate succinimidyl ester (CFDA-se). Viability of the yeast strains was not affected by pHâ¯3.4 and 3% (v/v) ethanol (EtOHpH3.4). 285â¯mM lactic acid (LApH3.4) reduced the specific growth rate (µmax) from 0.27-0.41â¯h-1 to 0.11-0.26â¯h-1 and the viability from 100% to 2.6-41.7% at 72â¯h of exposure in most yeast strains, except for two strains of C. glabrata. 150â¯mM acetic acid (AApH3.4) as well as the combinations (LAâ¯+â¯AA)pHâ¯3.4 and (LAâ¯+â¯AA+EtOH)pHâ¯3.4 reduced µmax to 0.0â¯h-1 and induced significant cell death for all the yeast strains. Exposed to (LAâ¯+â¯AA+EtOH)pHâ¯3.4, the most resistant yeast strains belonged to S. cerevisiae followed by P. kudriavzevii, whereas C. glabrata and K. marxianus were more sensitive. Strain variations were observed within all four species. When transferred to non-stress conditions, i.e. MYGP, pHâ¯5.6, after exposure to (LAâ¯+â¯AA+EtOH)pHâ¯3.4 for 6â¯h, 45% of the single cells of the most resistant S. cerevisiae strain kept their plasma membrane integrity, recovered their pHi to near physiological range (pHiâ¯=â¯6.1-7.4) and resumed proliferation after 3-24â¯h of lag phase. The results obtained are valuable in order to change processing conditions of the dough to favor the survival of preferable yeast species, i.e. S. cerevisiae and K. marxianus and inhibit opportunistic pathogen yeast species as C. glabrata.
Assuntos
Candida/efeitos dos fármacos , Grão Comestível/microbiologia , Alimentos Fermentados/microbiologia , Kluyveromyces/efeitos dos fármacos , Viabilidade Microbiana/efeitos dos fármacos , Pichia/efeitos dos fármacos , Saccharomyces cerevisiae/efeitos dos fármacos , Ácido Acético/farmacologia , Reatores Biológicos , Candida/crescimento & desenvolvimento , Candida/isolamento & purificação , Etanol/farmacologia , Fermentação , Kluyveromyces/crescimento & desenvolvimento , Kluyveromyces/isolamento & purificação , Ácido Láctico/farmacologia , Pichia/crescimento & desenvolvimento , Pichia/isolamento & purificação , Saccharomyces cerevisiae/crescimento & desenvolvimento , Saccharomyces cerevisiae/isolamento & purificação , Fermento Seco , Leveduras/isolamento & purificaçãoRESUMO
This study attempted to investigate the physiological response of six Lactobacillus brevis strains to hop stress, with and without the addition of Mn2+ or ethanol. Based on the use of different fluorescent probes, cell viability and intracellular pH (pHi) were assessed by fluorescence microscopy combined with flow cytometry, at the single cell level. The combined approach was faster than the traditional colony based method, but also provided additional information about population heterogeneity with regard to membrane damage and cell size reduction, when exposed to hop compounds. Different physiological subpopulations were detected under hop stress in both hop tolerant and sensitive strains. A large proportion of cells were killed in all the tested strains, but a small subpopulation from the hop tolerant strains eventually recovered as revealed by pHi measurements. Furthermore, a short term protection against hop compounds was obtained for both hop tolerant and sensitive strains, by addition of high concentration of Mn2+. Addition of ethanol in combination with hop compounds caused an additional short term increase in damaged subpopulation, but the subsequent growth suggested that the presence of ethanol provides a slight cross resistance toward hop compounds.
RESUMO
Beer is a hostile environment for most microorganisms, but some lactic acid bacteria can grow in this environment. This is primarily because these organisms have developed the ability to grow in the presence of hops. It has been speculated that hop resistance is inversely correlated to resistance against oxidation, and this would have great impact on the use of various disinfectants in the brewing industry. In this study, we cultivated bacteria under aerobic and anaerobic conditions, and then investigated the in situ outgrowth of individual cells into microcolonies on de Man Rogosa Sharpe (MRS) agar after exposure to the oxidizing agent peracetic acid (PAA). An automated microscope stage allowed us to analyse a much larger number of cells over extended periods of incubation. After PAA treatment, the lag time increased markedly, and extensive variation in morphology, µmax as well as stress resistance was observed between and within the tested Lactobacillus brevis strains. The results suggest that aerobic cultivation increased the oxidative stress tolerance in Lactobacillus brevis. The results also show that dead cells are randomly distributed in a microcolony and the majority of non-growing individual cells do not stain with a membrane impermanent dye (Propidium iodide), which indicates that PAA may not destroy the plasma membrane. In conclusion, the developed microscopic analysis of individual cells on MRS agar can provides faster results and more details of cell physiology compared to the traditional CFU method.
RESUMO
In the present work, an in vitro model of the gastrointestinal tract (GIT) was developed to obtain real-time observations of the pH homeostasis of single cells of probiotic Lactobacillus spp. strains as a measure of their physiological state. Changes in the intracellular pH (pHi) were determined using fluorescence ratio imaging microscopy (FRIM) for potential probiotic strains of Lactobacillus plantarum UFLA CH3 and Lactobacillus brevis UFLA FFC199. Heterogeneous populations were observed, with pHi values ranging from 6.5 to 7.5, 3.5 to 5.6 and 6.5 to 8.0 or higher during passage of saliva (pH 6.4), gastric (pH 3.5) and intestinal juices (pH 6.4), respectively. When nutrients were added to gastric juice, the isolate L. brevis significantly decreased its pH(i) closer to the extracellular pH (pH(ex)) than in gastric juice without nutrients. This was not the case for L. plantarum. This study is the first to produce an in vitro GIT model enabling real-time monitoring of pH homeostasis of single cells in response to the wide range of pH(ex) of the GIT. Furthermore, it was possible to observe the heterogeneous response of single cells. The technique can be used to determine the survival and physiological conditions of potential probiotics and other microorganisms during passage through the GIT.
Assuntos
Citosol/química , Suco Gástrico/química , Suco Gástrico/microbiologia , Homeostase , Lactobacillus plantarum/efeitos dos fármacos , Lactobacillus plantarum/fisiologia , Levilactobacillus brevis/fisiologia , Concentração de Íons de Hidrogênio , Viabilidade Microbiana/efeitos dos fármacos , Microscopia de Fluorescência/métodos , Imagem Óptica/métodosRESUMO
This study enables in situ studying of the growth and death of a large number of individual cells in a solid matrix. A wild type of Lactococcus lactis and several mutants with varying expression of GuaB was investigated. Large variability in the final size of individual microcolonies arising from clonal cells was observed. However, when growth was averaged over 16 locations in a specimen, the SEM was small and notable differences could be observed between the investigated strains, where mutants with lower expression of GuaB had a slower growth rate. The results show that the slow-growing mutants exhibited a lower fraction of dead cells, which indicate that slow-growing mutants are slightly more robust than the faster-growing strains. The large variability in the final size of individual microcolonies arising from clonal cells was quite surprising. We suggest that the control of the size of a microcolony is, at least partially, related to the actual microcolony depended on phenotypic heterogeneity. These findings are important to consider whenever a solid medium with discrete microcolonies is investigated.
Assuntos
Lactococcus lactis/fisiologia , Viabilidade Microbiana , Proteínas de Bactérias/biossíntese , Proteínas de Bactérias/genética , Meios de Cultura/química , Expressão Gênica , Variação Genética , Processamento de Imagem Assistida por Computador , Lactococcus lactis/crescimento & desenvolvimento , Microscopia , Proteínas Mutantes/biossíntese , Proteínas Mutantes/genética , FenótipoRESUMO
The effects of flow direction and shear stress on the adhesion of different strains of Listeria monocytogenes to fine polished stainless steel under liquid flow conditions were investigated. Furthermore, the relationship between cell surface properties and cell size and the initial adhesion rate (IAR) was studied. A method, including fluorescence microscopy and a flow perfusion system, was developed and used to examine the real-time initial cell adhesion of different L. monocytogenes species in situ to opaque surfaces under flow conditions. The results demonstrated that shear stress was the determining factor for the initial adhesion of L. monocytogenes under flow conditions. The flow direction in relation to the orientation of surface features (the scratches) could be disregarded. IARs were dependent on the shear stress and strain type. The strain EGDe, which had the lowest IAR, had the largest cell size, was the least hydrophobic and possessed the most electron-donating cell surface. Except for the L. monocytogenes strain EGDe, no clear correlations were found between the IAR and cell surface properties, or cell size. In conclusion, many factors may be involved in determining the initial adhesion of L. monocytogenes to stainless steel under flow conditions. Two of the main factors are flow rate/shear stress and strain specificity.
Assuntos
Aderência Bacteriana/fisiologia , Biofilmes/crescimento & desenvolvimento , Listeria monocytogenes/crescimento & desenvolvimento , Indústria Alimentícia , Interações Hidrofóbicas e Hidrofílicas , Resistência ao Cisalhamento , Aço Inoxidável , Propriedades de Superfície , Movimentos da ÁguaRESUMO
In this study we have modified the BioPhotonics workstation (BWS), which allows for using long working distance objective for optical trapping, to include traditional epi-fluorescence microscopy, using the trapping objectives. We have also added temperature regulation of sample stage, allowing for fast temperature variations while trapping. Using this modified BWS setup, we investigated the internal pH (pH(i)) response and membrane integrity of an optically trapped Saccharomyces cerevisiae cell at 5 mW subject to increasing temperatures. The pH(i) of the cell is obtained from the emission of 5-(and-6)-carboxyfluorescein diacetate, succinimidyl ester, at 435 and 485 nm wavelengths, while the permeability is indicated by the fluorescence of propidium iodide. We present images mapping the pH(i) and permeability of the cell at different temperatures and with enough spatial resolution to localize these attributes within the cell. The combined capability of optical trapping, fluorescence microscopy and temperature regulation offers a versatile tool for biological research.
Assuntos
Resposta ao Choque Térmico , Espaço Intracelular/química , Fenômenos Ópticos , Óptica e Fotônica/instrumentação , Saccharomyces cerevisiae/citologia , Saccharomyces cerevisiae/fisiologia , Calibragem , Sobrevivência Celular , Concentração de Íons de Hidrogênio , TemperaturaRESUMO
Internal pH regulation is vital for many cell functions, including transport mechanisms and metabolic enzyme activity. More specifically, transport mechanisms are to a wide degree governed by internal pH distributions. We introduce the term standard deviation of the intracellular pH (s.d.(pH(int))) to describe the internal pH distributions. The cellular pH distributional response to external stress such as heat has not previously been determined. In this study, the intracellular pH (pH(i)) and the s.d.(pH(int)) of Saccharomyces cerevisiae cells exposed to supralethal temperatures were measured using fluorescence ratio imaging microscopy (FRIM). An exponential decline in pH(i) was observed after an initial small decline. For the first time, we report the use of FRIM for determining in vivo plasma membrane proton permeability coefficients in yeast. Furthermore, the exponential decay of pH(i) and the rupture of the cell plasma membrane, as measured by propidium iodide staining, at 70°C were not simultaneous but were separated by a significant temporal difference. Finally, a nonlinear relationship between the pH(i) and s.d.(pH(int)) was found; i.e. the s.d.(pH(int)) was significantly more sensitive to supralethal temperatures than pH(i). s.d.(pH(int)) is therefore proposed as an early health/vitality indicator in S. cerevisiae cells exposed to heat stress.
Assuntos
Equilíbrio Ácido-Base/fisiologia , Permeabilidade da Membrana Celular/fisiologia , Líquido Intracelular/química , Saccharomyces cerevisiae/química , Fluoresceínas , Nível de Saúde , Concentração de Íons de Hidrogênio , Microscopia de Fluorescência/métodos , Propídio , Succinimidas , Temperatura , Fatores de TempoRESUMO
The aim of this study was to investigate the response of Campylobacter jejuni at single-cell level when exposed to different concentrations of chlorine dioxide (ClO(2)). The parameter of choice, intracellular pH (pH(i)), was determined by using fluorescence ratio imaging microscopy with a pH-sensitive, ratiometric 5(6)-carboxyfluorescein diacetate succinimidyl ester probe. In addition, the culturability expressed in colony counts was determined. Our results revealed that several subpopulations with different physiological states, as judged by their pH(i), were created by ClO(2) treatment. The greater the concentration of ClO(2), the smaller the subpopulation of healthy cells with pH(i) > 6.8 and the smaller the colony count as determined on nonselective agar plates. ClO(2) at concentrations (60 ppm) induced injuries that resulted in complete loss of culturability and adversely affected the ability to resuscitate under subsequent more favorable conditions. The presence of injured cells in food could present a risk for public health. Additional hurdles have to be included in food preservation to suppress the survival and recovery of injured cells.
Assuntos
Campylobacter jejuni/efeitos dos fármacos , Campylobacter jejuni/metabolismo , Compostos Clorados/farmacologia , Desinfetantes/farmacologia , Óxidos/farmacologia , Campylobacter jejuni/crescimento & desenvolvimento , Compostos Clorados/administração & dosagem , Contagem de Colônia Microbiana , Desinfetantes/administração & dosagem , Relação Dose-Resposta a Droga , Corantes Fluorescentes/química , Conservação de Alimentos/métodos , Concentração de Íons de Hidrogênio , Viabilidade Microbiana , Microscopia de Fluorescência , Óxidos/administração & dosagem , Análise de Célula Única , Especificidade da EspécieRESUMO
The effect of a 1070-nm continuous and pulsed wave ytterbium fiber laser on the growth of Saccharomyces cerevisiae single cells is investigated over a time span of 4 to 5 h. The cells are subjected to optical traps consisting of two counterpropagating plane wave beams with a uniform flux along the x, y axis. Even at the lowest continuous power investigated-i.e., 0.7 mW-the growth of S. cerevisiae cell clusters is markedly inhibited. The minimum power required to successfully trap single S. cerevisiae cells in three dimensions is estimated to be 3.5 mW. No threshold power for the photodamage, but instead a continuous response to the increased accumulated dose is found in the regime investigated from 0.7 to 2.6 mW. Furthermore, by keeping the delivered dose constant and varying the exposure time and power-i.e. pulsing-we find that the growth of S. cerevisiae cells is increasingly inhibited with increasing power. These results indicate that growth of S. cerevisiae is dependent on both the power as well as the accumulated dose at 1070 nm.
Assuntos
Saccharomyces cerevisiae/fisiologia , Saccharomyces cerevisiae/efeitos da radiação , Proliferação de Células/efeitos da radiação , Relação Dose-Resposta à Radiação , Luz , Doses de RadiaçãoRESUMO
We generated monoclonal scFv (single chain variable fragment) antibodies from an antibody phage display library towards three small synthetic peptides derived from the sequence of αs1-casein. Key difficulties for selection of scFv-phages against small peptides were addressed. Small peptides do not always bind efficiently to passive adsorption surfaces, and we developed a simple method to quantify the binding capacity of surfaces with the peptides. Background binding (the binding of scFvs to the background matrix) is an obstacle for successful selection, and we evaluated two methods that drastically reduced the background binding. An optimized method therefore enabled a panning procedure where the specific (peptide binding) scFv-phages were always dominant. Using 15-mer peptides immobilized on Nunc Immobilizer Streptavidin plates, we successfully generated scFvs specifically against them. The scFvs were sequenced and characterized, and specificity was characterized by ELISA. The methods developed in this study are universally applicable for antibody phage display to efficiently produce antibody fragments against small peptides.
Assuntos
Especificidade de Anticorpos , Biblioteca de Peptídeos , Anticorpos de Cadeia Única/química , Sequência de Aminoácidos , Anticorpos Imobilizados , Ensaio de Imunoadsorção Enzimática , Humanos , Dados de Sequência MolecularRESUMO
Mycobacterium avium subsp. paratuberculosis (MAP) is the etiologic agent of Johne's disease; moreover, it seems to be implicated in the development of Crohn's disease in humans. In the present study, fluorescence ratio imaging microscopy (FRIM) was used to assess changes in intracellular pH (pH(i)) of one strain of MAP after exposure to nisin and neutralized cell-free supernatants (NCSs) from five bacteriocin-producing lactic acid bacteria (LAB) with known probiotic properties. The evaluation of pH(i) by FRIM provides information about the physiological state of bacterial cells, bypassing the long and problematic incubations needed for methods relying upon growth of MAP such as determination of colony forming units. The FRIM results showed that both nisin and the cell-free supernatant from Lactobacillus plantarum PCA 236 affected the pH(i) of MAP within a few hours. However, monitoring the population for 24h revealed the presence of a subpopulation of cells probably resistant to the antimicrobial compounds tested. Use of nisin and bacteriocin-producing LAB strains could lead to new intervention strategies for the control of MAP based on in vivo application of probiotic cultures as feed additives at farm level.
Assuntos
Antibacterianos/farmacologia , Bacteriocinas/farmacologia , Lactobacillus/química , Mycobacterium avium subsp. paratuberculosis/efeitos dos fármacos , Nisina/farmacologia , Probióticos/farmacologia , Resistência Microbiana a Medicamentos , Concentração de Íons de Hidrogênio , Microscopia de Fluorescência , Mycobacterium avium subsp. paratuberculosis/fisiologiaRESUMO
As mild decontamination treatments are gaining more and more interest due to increased consumer demands for fresh foods, it is of great importance to establish the influence of decontamination treatments on the subsequent bacterial behaviour under suboptimal storage conditions. For this purpose Campylobacter jejuni cells treated with lactic acid (LA, 3% lactic acid, pH 4.0, 2 min) or chlorine dioxide (ClO(2), 20 ppm, 2 min) were inoculated in Bolton broth (pH 6.0) and incubated under 80% O(2)/20% N(2), 80% CO(2)/20% N(2), air or micro-aerophilic (10% CO(2)/85% N(2)/5% O(2)) atmosphere, at 4 degrees C during 7 days. Treatment with water served as a control. The most suppressive atmosphere for the survival of C. jejuni was O(2)-rich atmosphere, followed by air, micro-aerophilic and CO(2)-rich atmosphere. The survival of C. jejuni was dependent on the type of initial decontamination treatment, with water treated cells showing the greatest survival followed by LA and ClO(2) treated cells. Intracellular pH (pH(i)) of individual C. jejuni cells was determined using Fluorescence Ratio Imaging Microscopy (FRIM). At all tested conditions, different subpopulation of the cells could be distinguished based on their pH(i) values. The pH(i) response was independent on the surrounding atmosphere since similar distribution of the subpopulations was observed for all tested atmospheres. However, the pH(i) response was dependent on the initial decontamination treatment. The investigation of intracellular parameters gave an insight into pathogen behaviour under stressful conditions at intracellular level. The results obtained in this study highlighted the importance of combining decontamination technologies with subsequent preservation techniques to the control survival and growth of foodborne pathogens.
Assuntos
Campylobacter jejuni/efeitos dos fármacos , Compostos Clorados/farmacologia , Descontaminação/métodos , Conservação de Alimentos/métodos , Ácido Láctico/farmacologia , Viabilidade Microbiana/efeitos dos fármacos , Óxidos/farmacologia , Estresse Fisiológico , Ar , Campylobacter jejuni/fisiologia , Dióxido de Carbono/fisiologia , Manipulação de Alimentos , Microbiologia de Alimentos , Concentração de Íons de Hidrogênio , Microscopia de Fluorescência , Oxigênio/fisiologia , Temperatura , ÁguaRESUMO
A novel method to monitor in situ hydrolyzable casein fragments during cheese ripening by using immunofluorescent labeling and confocal laser scanning microscopy (CLSM) was developed. Monoclonal single chain variable fragments of antibody (scFvs) were generated by antibody phage display toward three small synthetic peptides of the alpha(s1)-casein sequence. These peptides traverse enzymatic cleavage sites of casein during cheese ripening. The specificity of the generated anti-peptide antibodies was determined by ELISA and Western blot. Finally, an immunofluorescent labeling protocol was successfully developed for the detection of scFvs binding to different alpha(s1)-casein fragments inside a cheese matrix by CLSM. To our knowledge, this is the first demonstrated immunofluorescent labeling method for in situ analysis of proteolysis phenomena in the cheese matrix. Additionally, this technique offers a high potential to study in situ dynamic spatial changes of target components in complex food systems.
Assuntos
Anticorpos Monoclonais/análise , Caseínas/química , Queijo/análise , Microscopia Confocal/métodos , Biblioteca de Peptídeos , Hidrólise , Anticorpos de Cadeia Única/análise , Anticorpos de Cadeia Única/genética , Anticorpos de Cadeia Única/metabolismoRESUMO
The aim of the study was to determine intracellular pH (pH(i)) as an indicator of the physiological state of two Campylobacter jejuni strains (603 and 608) at the single cell level after bactericidal treatment with lactic acid (3% v/v lactic acid, pH 4.0, 0.85% w/v NaCl) and during recovery and survival using Fluorescence Ratio Imaging Microscopy (FRIM). After exposure to lactic acid solution a decline in pH(i) to 5.5 (FRIM detection limit) was observed in the majority of cells (75-100%) within 2 min. The enumeration data revealed that after 2 min of lactic acid exposure, approx. 90% of the initial population became unculturable. In the following 10 min of exposure, a further decrease in the cell count was observed resulting in 3.53 and 3.21 log CFU/ml reduction of culturable cells at the end of the treatment. On the contrary, the FRIM results revealed that the subpopulations with pH(i)>5.5 increased between 2 and 12 min of exposure to lactic acid. Removing the acid stress and incubating the cells suspension under the more favourable conditions resulted in an immediate increase in cell population with pH(i)>pH(ex) for both C. jejuni strains. Further 24 h incubation at 37 degrees C resulted in increased pH(i) and colony count (recovery study). On the contrary, 24 h incubation at suboptimal temperature of 4 degrees C, showed pH(i) decrease to pH(ex)=6.0 (no pH gradient) in the whole population of C. jejuni cells. Rather than dying, cells exposed for longer time (72 and 120 h) to 4 degrees C increased the subpopulation of the cells with positive pH gradient, mostly comprised of the cells with DeltapH>0.5, indicating the ability of C. jejuni cells to regulate their metabolic activity under suboptimal conditions.
Assuntos
Adaptação Fisiológica , Campylobacter jejuni/fisiologia , Descontaminação , Concentração de Íons de Hidrogênio , Ácido Láctico , Viabilidade Microbiana , Campylobacter jejuni/crescimento & desenvolvimento , Contagem de Colônia Microbiana , Microscopia de Fluorescência/métodos , Estresse Fisiológico , TemperaturaRESUMO
Responses of Listeria innocua and Lactobacillus delbrueckii subsp. bulgaricus to a rapid change in extracellular pH (pHex) from pHex 6 to a range of concentrations down to pHex 3.0 were examined, using HCl and lactic acid (LA) as acidulants. A new fluorescent probe 5-(and-6)-carboxy-2', 7'-dichlorofluorescein diacetate succinimidyl ester (CDCFDA-SE) was employed that enabled reliable measurements of intracellular pH (pHi) to a minimum pHi of 4.0. Changes in pHi and H+ fluxes from immobilised bacteria were measured using fluorescence ratio imaging microscopy (FRIM) and a non-invasive ion flux measuring technique (MIFE), respectively. L. innocua maintained a relatively constant pHi of 5.5-6.1 at pHex 4 and 5 via H+ extrusion. In contrast, L. delbrueckii subsp. bulgaricus progressively lowered pHi towards pHex over the entire pHex range examined. The type of acidulant used influenced pH regulation with both pHi and H+ -fluxes being more severely affected by LA compared to HCl. Overall, our data demonstrated different adaptive strategies in these two bacteria. While L. innocua expels protons to maintain a constant pHi, L. delbrueckii subsp. bulgaricus allows proton entry after acidic treatment so that pHi follows pHex.
Assuntos
Ácido Clorídrico/farmacologia , Ácido Láctico/farmacologia , Lactobacillus delbrueckii/efeitos dos fármacos , Listeria/efeitos dos fármacos , Adaptação Fisiológica , Microbiologia de Alimentos , Homeostase , Concentração de Íons de Hidrogênio , Lactobacillus delbrueckii/crescimento & desenvolvimento , Lactobacillus delbrueckii/fisiologia , Listeria/crescimento & desenvolvimento , Listeria/fisiologia , Microscopia de Fluorescência/métodos , Modelos BiológicosRESUMO
A mixed culture of single cells of Listeria monocytogenes and the bacteriocin producing Leuconostoc carnosum 4010 showed growth inhibition of L. monocytogenes, although the intracellular pH (pHi) of L. monocytogenes followed by fluorescence ratio imaging microscopy was not affected. Furthermore, L. monocytogenes was exposed to the bacteriocins leucocins 4010 and nisin either in a liquid filled chamber or on the surface of an agar containing bacteriocins. Both bacteriocins caused dissipation of the pH gradient in L. monocytogenes and the effect was clearly dependent on the matrix, as the decrease in pHi occurred much more rapidly in liquid than in agar.
Assuntos
Bacteriocinas/farmacologia , Leuconostoc/fisiologia , Listeria monocytogenes/efeitos dos fármacos , Listeria monocytogenes/crescimento & desenvolvimento , Bacteriocinas/metabolismo , Técnicas de Cocultura , Contagem de Colônia Microbiana , Matriz Extracelular/efeitos dos fármacos , Concentração de Íons de Hidrogênio , Cinética , Leuconostoc/citologia , Leuconostoc/crescimento & desenvolvimento , Listeria monocytogenes/citologiaRESUMO
We investigated protein and gene expression in the lag phase of Lactococcus lactis subsp. lactis CNRZ 157 and compared it to the exponential and stationary phases. By means of two-dimensional polyacrylamide gel electrophoresis, 28 highly expressed lag-phase proteins, implicated in nucleotide metabolism, glycolysis, stress response, translation, transcription, cell division, amino acid metabolism, and coenzyme synthesis, were identified. Among the identified proteins, >2-fold induction and down-regulation in the lag phase were determined for 12 proteins in respect to the exponential phase and for 18 proteins in respect to the stationary phase. Transcriptional changes of the lag-phase proteins in L. lactis were studied by oligonucleotide microarrays. Good correlation between protein and gene expression studies was demonstrated for several differentially expressed proteins, including nucleotide biosynthetic enzymes, adenylosuccinate synthase (PurA), IMP dehydrogenase (GuaB), and aspartate carbamoyl transferase (PyrB); heat-shock protein DnaK; serine hydroxymethyl transferase (GlyA); carbon catabolite control protein (CcpA); elongation factor G (FusA); and cell division protein (FtsZ).