Açaí Flan, A Functional Food with Lacticaseibacillus rhamnosus HN001 Probiotic: Physicochemical Characteristics, Probiotic Survival, Sensory Acceptance and Consumer Perception.

Research background: Açaí berry is rich in antioxidant compounds and is therefore closely associated with beneficial health effects. In this study, we aim to investigate the potential of using Lacticaseibacillus rhamnosus HN001 as a probiotic culture on açaí flan. Experimental approach: The chemical composition, physicochemical and microbiological characteristics, and sensory acceptance during refrigerated storage (5 °C for 42 days) of flan were investigated. In addition, the consumer perception of the product was evaluated using word association when consumers were shown a photo of the product with or without the added ingredients accompanied with a brief description of the product. Results and conclusions: The flan had a suitable chemical composition, mainly carbohydrates and proteins, probiotic viability reached 8 log CFU/g in the product and 4 log CFU/g after gastrointestinal simulation, typical açaí coloration, significant antioxidant activity and high sensory acceptability. The information about the ingredients and properties of the products increased the health value and positive feelings of the consumers towards the product. Novelty and scientific contribution: Açaí flan has proven to be a suitable carrier for L. rhamnosus HN001 as a probiotic culture, further enhancing the characteristic beneficial properties of the fruit. Therefore, combining this information with marketing strategies that inform consumers about the benefits of the product can further improve its acceptance. As far as we know, this is the first study on açaí flan with added probiotic culture.

Maturation of honey from Uruçú-Amarela (Melipona mondury): Metagenomics, metabolomics by NMR 1H, physicochemical and antioxidant properties.

The objective of this study was to characterize the microbiota biodiversity of Uruçú-Amarela honey through metagenomics. Furthermore, the impact of maturation temperatures (20 and 30 °C) and time (0-180 days) on the physicochemical and antioxidant properties was investigated. 1H NMR was performed to verify metabolites formed during maturation. Uruçú-Amarela honey was mainly composed by lactic acid bacteria and osmophilic yeasts of genus Zygosaccharomyces. Maturation at 30 °C led to a higher fermentation activity, resulting in greater carbohydrate consumption, ethanol formation (0.0-0.6 %) and increased acidity (34.78-45.74 meq/kg) over the 180 days. It also resulted in honey with higher brown color (a* 0.7 to 3.89, b* 17.50-25.29) and antioxidant capacity, corroborating that the maturation is a suitable preservation technique for stingless bee honey, because it does not cause negative changes as it extends the shelf life of the stingless bee honey.

Mandacaru fruit pulp (Cereus jamacaru D.C.) as an adjunct and its influence on Beer properties.

Beer is a complex product due to its raw materials (malt, hops, yeast, and water). Beer production can also use other matters as adjuncts. This study investigated the influence of Mandacaru fruit pulp (MFP) as an adjunct on volatile and phenolic compounds, and antioxidant properties of Beer. Worts were produced using four treatments including a control. Fermentations were conducted for 10 days at 18 °C using yeast Lachancea spp, maturated at 3 °C for 15 days, and bottled at 20 °C for 15 days. All compounds were evaluated by HPLC, and GC-MS. Worts' supplementation influenced the volatile and phenolic profile and increased the antioxidant activity of wort and Beer. Beers A (100 g of MFP/L), B (200 g of MFP/L), and C (300 g of MFP/L) presented higher ethanol and glycerol content. Beer C contained the highest antioxidant activity and total phenolic content. Worts' supplemented with MFP increased aroma formation.

Fermented beverage based on water-soluble soybean extract / Bebida fermentada à base de extrato hidrosolúvel de soja

Functional foods are those that, beyond basic nutrition, promote health benefits. This study aimed to produce a fermented beverage from a water-soluble soybean extract and low-lactose semi-skimmed milk. The beverage was prepared using a blend (v/v) containing 62% water-soluble soybean extract, 33% low-lactose semi-skimmed milk, and 5% of an inoculum containing Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus salivarius subsp. thermophilus. The fermentation was carried out with monitoring of the lactic acid bacteria (LAB) counts, pH, and titrable acidity (% lactic acid). The consumption-ready fermented beverage was added of strawberry syrup and posteriorly characterized by its centesimal composition and sensory attributes as appearance, aroma, flavour, texture, overall impression, and purchase intent. Additionally, the LAB count and presence of pathogens in the final product were evaluated. After 14 h of fermentation, the refrigerated product presented L. bulgaricus and S. thermophilus counts of 8.5 × 103 and 7.4 × 107 CFU/mL, respectively. The product contained 14.93% carbohydrates, 0.76% fats, 0.96% ash, 22.76% totalsolids, and 2.19% protein. Because the product did not present any pathogens, it was adequate according to the standards of microbiological safety determined by Brazilian legislation. Regarding the sensory evaluation, the panelists assigned a mean score of 7.0 for the attributes evaluated, indicating 'liked moderately' to the drink. In addition, 61% of the evaluators responded that they 'certainly or probably would buy' the product if it were available on the market.

Alimentos funcionais são aqueles que, além da nutrição básica, promovem benefícios à saúde. Este trabalho teve como objetivo produzir uma bebida fermentada a partir de extrato hidrosolúvel de soja (EHS) e leite semidesnatado com baixa lactose. A bebida foi preparada utilizando uma mistura (v/v) contendo 62% de EHS, 33% de leite semidesnatado com baixa lactose e 5% de inóculo contendo Lactobacillus delbrueckii subsp. Bulgaricus e Streptococcus salivarius subsp. Thermophilus. A fermentação foi acompanhada durante 14 horas, através das contagens das bactérias ácido lácticas, pH e a acidez titulável (% de ácido láctico). A bebida fermentada pronta para consumo foi adicionada de uma calda de morango e caracterizada por sua composição centesimal, bem como por avaliação sensorial para os atributos: aparência, aroma, sabor, impressão global e intenção de compra. Além disso, a contagem das bactérias lácticas e presença de patógenos no produto foi avaliada. Após 14 horas de fermentação, o produto final refrigerado apresentou contagens de 8,5x103 UFC/mL e 7,4x107 UFC/mL para as bactérias L. bulgaricus e S. thermophilus respectivamente. O produto apresentou 14,93% de carboidratos, 0,76% de gordura, 0,96% de cinzas, 22,76% de sólidos totais e 2,19% de proteína. O produto não apresentou quaisquer patógenos, portanto, foi adequado aos padrões determinados pela legislação brasileira quanto à segurança microbiólogica. Com relação à avaliação sensorial, os provadores atribuíram em média nota 7,0 para todos os parâmetros avaliados, indicando que "gostarammoderadamente" da bebida. Ademais 61% dos avaliadores apontaram que "certamente ou provavelmente comprariam" o produto se estivesse disponível no mercado.

Sociograma das representações afetivas da matriz de identidade

Neste trabalho, descreve-se o uso do sociograma em sessões de psicoterapia individual. O sociograma das representações afetivas da matriz de identidade é um diagrama das primeiras figuras internalizadas com que o indivíduo conviveu na infância e tem um fim terapêutico(AU)

Prevalência de leveduras do gênero candida em secreçäo vaginal / Prevelence of yeasts of candida sp at vaginal secretion

Os autores estabeleceram a prevalência de Candida sp. em secreçao vaginal de 85 mulheres, com idade entre 16 a 68 anos, através de cultura de ágar Sabouraud-cloranfenicol, microscopia de exame a fresco e esfregaço corado pelo método de Gram. Verificaram a presença da levedura em 24,71 por cento das amostras estudadas. Observaram que entre as culturas positivas, 47,62 por cento dos casos analisados pertenciam a mulheres na faixa etária entre 20 e 30 anos e entre pacientes grávidas a positividade foi de 61,ll por cento. Concluíram que as leveduras do gênero Candida sao de expressiva prevalência em secreçao vaginal e que a gravidez é um fator de predisposiçao de grande importância.