Current practices in physical fitness assessment and monitoring among coaches of individual and team sports: a survey in Portugal, Spain, and Romania.

The objective of this study was to characterize surveyed coaches and elucidate the practices of physical fitness assessment and monitoring for both male and female athletes across three countries. A total of 165 coaches participated by completing a comprehensive 32-question survey. Pre-season assessments are a priority for coaches, with a significant range from 60.5% to 87.7% in Romania, while Portuguese and Spanish coaches tend to prefer testing during the competition (26.3% and 16.9%, respectively). Portuguese and Spanish coaches predominantly favor aerobic tests (50% and 46.8% respectively), whereas Romanian coaches exhibit a preference for sprint (56.9%) and skill tests (52.3%). Notably, change of direction tests are less commonly employed, ranging from 10.5% to 21% across the countries. In terms of exercise intensity determination, Portuguese coaches predominantly employ maximal heart rate (31.6%), while Spanish coaches often rely on the 220-age formula or perceived exertion (27.4%). For strength assessment, Portuguese coaches lean towards direct (34.2%) or estimated (31.6%) maximal repetition methods. When it comes to maximal speed sprint, Portuguese and Romanian coaches show preference (50% and 43.1% respectively), while Spanish coaches exhibit a relative lack of emphasis on individualized speed measures (37.1%). Perceptual scales are the preferred method for recovery monitoring, with adoption rates of 57.9% in Portugal, 53.2% in Spain, and 44.6% in Romania. In summary, this study underscores the distinct assessment and monitoring practices employed by coaches in Portugal, Spain, and Romania. These findings are in alignment with established literature standards, highlighting the diversity of approaches used in different countries.

Relationships between physical fitness and match running demands during a futsal congested-weeks training camp.

This study examines the relationships between body composition, anthropometry and physical fitness measures, and internal and external load (match physical and running demands) during a congested period of an overseas futsal training camp. Eleven under-20 national futsal players participated in a eleven-day training camp. During the matches, exercise heart rate (HR) and locomotion profiles were recorded via a Polar Team Pro system as the players' internal and external load. The friendly matches were scheduled on the training camp's 2nd, 4th, 6th, 8th, and 10th days. A repeated-measures analysis of variance (ANOVA) and Pearson's correlation coefficient were used for statistical analysis. The results revealed significant differences in sprint (F = 2.74, p = 0.04, η2 = 0.21), accelerations (F = 3.63, p = 0.04, η2 = 0.27), and decelerations (F = 2.73, p = 0.04, η2 = 0.21) performance between the five matches (p < 0.05). Also, the overall body composition and physical fitness measures had large to very large correlations with match running demands during congested periods of futsal competition (p < 0.05). In conclusion, the baseline body composition and the overall physical fitness measures may be essential factors in match running demands during a congested futsal match period. Futsal players who perform better in the 30-15 Intermittent Fitness Test can sustain a greater volume of high-intensity accelerations during a congested period.

Assessing physical fitness adaptations in collegiate male soccer players through training load parameters: a two-arm randomized study on combined small-sided games and running-based high-intensity interval training.

Objective: To evaluate the effects of a 4-week intervention combining small-sided games (SSGs) and high-intensity interval training (HIIT) on physical fitness in collegiate male soccer players. Methods: Twenty-one soccer players were randomly assigned to either the HIIT + SSGs group (n = 11) or a control group (n = 10). Physical fitness was assessed at baseline and 1-week post-intervention, including countermovement jump (CMJ), change of direction (COD) test, sprint test, repeated sprint ability (RSA) test, and 30-15 Intermittent Fitness Test (30-15IFT). The intervention comprised eight sessions over 4 weeks: four SSGs and four HIIT. Results: The intervention group showed small to moderate improvements: mean RSA improved by 4.5% (p = 0.07), CMJ increased by 3.2% (p = 0.12), and 30-15IFT scores enhanced by 6.8% (p = 0.09). Key predictors of group membership included heart rate load per minute (OR 1.602) and various GPS variables. Conclusion: The 4-week intervention combining SSGs with HIIT did not produce statistically significant improvements in most physical fitness variables compared to the control group. Although there were positive trends in variables such as RSA and 30-15IFT, these changes were modest and not statistically significant. The results suggest that while the combined SSGs and HIIT approach shows potential, its impact on physical fitness over a 4-week period is limited, with some variables, like CMJ, even showing decreases.

The Influence of Playing Position on Physical, Physiological, and Technical Demands in Adult Male Soccer Matches: A Systematic Scoping Review with Evidence Gap Map.

BACKGROUND: There has been an increase in studies examining the demands of soccer relative to each playing position in recent years. Understanding the physical, physiological, and technical demands on soccer players according to their positional role during competitive matches is necessary to understand match requirements and develop position-specific training practices. Thereby, there is a clear need to synthesize the information on the different profiles of each playing position. OBJECTIVE: This review aimed to organize the literature investigating physical, physiological, and technical demands according to playing positions and provide a framework to identify gaps and suggestions for future studies. METHODS: A systematic search was conducted in October 2023 using four electronic databases: Web of Science, SPORTDiscus, PubMed and Scopus. The review followed PRISMA 2020 guidelines and the PRISMA-ScR extension for Scoping Reviews. The studies were included if the sample comprised adult male soccer players categorized from Tier 3 to Tier 5 (i.e., highly trained/national level, elite/international level, or world class) and compared the physical, physiological, or technical parameters across playing positions. RESULTS: A total of 178 studies met the inclusion criteria and were included in the review. The number of teams, players, and matches analyzed per study varied considerably. Although a range of classifications were reported across studies, 59% of studies classified players as central defenders, full-backs, central midfielders, wide midfielders, and forwards. The findings suggests that central and external midfielders, and external defenders cover greater total and high-speed distance than forwards or central defenders. Sprint distance was higher in external midfielders versus all other positions. Defenders and central midfielders perform more passes than external midfielders and forwards. Heart rate was the most commonly reported physiological variable across playing positions. When expressed as a percentage of maximal heart rate, midfielders presented higher mean values than all other playing positions. CONCLUSION: This scoping review demonstrates that there are differences in the demands on players across playing positions in soccer. Training practices in soccer should be based on the specific requirements of each positional role to ensure players can fulfill their tactical responsibilities during the game.

Material Performance Evaluation for Customized Orthoses: Compression, Flexural, and Tensile Tests Combined with Finite Element Analysis.

Orthoses are commonly used for treating injuries to improve the quality of life of patients, with customized orthoses offering significant benefits. Additive manufacturing, especially fused deposition modelling, enhances these benefits by providing faster, more precise, and more comfortable orthoses. The present study evaluates nine polymeric materials printed in horizontal and vertical directions by assessing their performance through compressive, flexural, and tensile tests. Among all materials, polycarbonate, polylactic acid, and ULTEMTM 1010 showed the most promising results, not only because they had the highest mechanical values, but also due to their minimal or no difference in performance between printing directions, making them advantageous in orthoses fabrication. Based on this, a finite element model of an ankle-foot orthosis was developed to simulate the deformation, strain, and stress fields under static conditions. The findings aim to optimize material selection for orthotic fabrication, where ULTEMTM 1010 is presented as the material with improved performance and durability.

Functionally Graded Materials and Structures: Unified Approach by Optimal Design, Metal Additive Manufacturing, and Image-Based Characterization.

Functionally Graded Materials (FGMs) can outperform their homogeneous counterparts. Advances in digitalization technologies, mainly additive manufacturing, have enabled the synthesis of materials with tailored properties and functionalities. Joining dissimilar metals to attain compositional grading is a relatively unexplored research area and holds great promise for engineering applications. Metallurgical challenges may arise; thus, a theoretical critical analysis is presented in this paper. A multidisciplinary methodology is proposed here to unify optimal design, multi-feed Wire-Arc Additive Manufacturing (WAAM), and image-based characterization methods to create structure-specific oriented FGM parts. Topology optimization is used to design FGMs. A beam under pure bending is used to explore the layer-wise FGM concept, which is also analytically validated. The challenges, limitations, and role of WAAM in creating FGM parts are discussed, along with the importance of numerical validation using full-field deformation data. As a result, a conceptual FGM engineering workflow is proposed at this stage, enabling digital data conversion regarding geometry and compositional grading. This is a step forward in processing in silico data, with a view to experimentally producing parts in future. An optimized FGM beam, revealing an optimal layout and a property gradient from iron to copper along the build direction (bottom-up) that significantly reduces the normal pure bending stresses (by 26%), is used as a case study to validate the proposed digital workflow.

Monitoring the detrimental impact of congested training periods on the strength levels and landing forces of young female aerobic gymnastics.

Identifying indicators of non-functional overreaching during periods of increased training volume and/or intensity is particularly relevant for understanding the detrimental impacts incurred, as well as how these factors contribute to heightened injury risks among exposed athletes. This study aimed to compare the effects of a congested training period versus a standard training period on the strength levels and landing forces of female young aerobic gymnastics athletes. A prospective cohort study design was implemented, spanning four weeks. Fifty athletes (aged 16.2 ± 1.1 years old) at a trained/developmental level, competing at the regional level, were observed throughout the study. During two of these weeks (specifically weeks 2 and 3), half of the group was subjected to a congested training period consisting of six sessions per week (HTF), while the other half continued with their regular four sessions per week (STF). During each week of observation, participants underwent evaluation using the countermovement jump test (CMJ), squat jump test (SJ), and the leg land and hold test (LHT), with measurements taken on a force platform. The main outcomes repeatedly observed over the four weeks were CMJ peak landing force, CMJ peak power, SJ peak power, SJ maximum negative displacement, LHT time to stabilization, and LHT peak drop landing force. Significant interactions (time*group) were observed in CMJ peak power (p < 0.001), CMJ peak landing force (p < 0.001), SJ peak power (p < 0.001), SJ maximum negative displacement (p < 0.001), LHT time to stabilization (p < 0.001), and LHT peak drop landing force (p < 0.001). Furthermore, the results of the final assessment revealed significantly lower CMJ peak power (p = 0.008) and SJ peak power (p = 0.002) in the HTF group compared to the STF group. Additionally, significantly higher values of CMJ peak landing force (p = 0.041), SJ maximum negative displacement (p = 0.015), and LHT peak drop landing force (p = 0.047) were observed in the HTF group compared to the STF group. In conclusion, the increase in training frequency over two weeks significantly contributed to declines in neuromuscular power performance and peak landing forces. This indicates that intensified training periods may acutely expose athletes not only to performance drops but also to an increased risk of injury due to reduced capacity to absorb landing forces.

Subcycle dynamics of excitons under strong laser fields.

Excitons play a key role in the linear optical response of two-dimensional (2D) materials. However, their role in the nonlinear response to intense, nonresonant, low-frequency light is often overlooked as strong fields are expected to tear the electron-hole pair apart. Using high-harmonic generation as a spectroscopic tool, we theoretically study their formation and role in the nonlinear optical response. We show that the excitonic contribution is prominent and that excitons remain stable even when the driving laser field surpasses the strength of the Coulomb field binding the electron-hole pair. We demonstrate a parallel between the behavior of strongly laser-driven excitons in 2D solids and strongly driven Rydberg states in atoms, including the mechanisms of their formation and stability. Last, we show how the excitonic contribution can be singled out by encapsulating the 2D material in a dielectric, tuning the excitonic energy and its contribution to the high-harmonic spectrum.

Central Venous Catheter Misplacement Into Pleural Cavity Causing Hypertensive Pleural Effusion: A Case Report.

Central venous catheter (CVC) insertion is a routine procedure in the management of critically ill patients. We report a clinical case of inadvertent placement of an internal jugular vein CVC into the right pleural cavity, despite employing clinical and imaging-based techniques to ensure proper catheter positioning. Infusion of fluids and vasopressors through this misplaced catheter led to hypertensive pleural effusion and subsequent cardiorespiratory arrest. Return of spontaneous circulation was achieved after two cycles of cardiopulmonary resuscitation. While multiple imaging modalities are recommended for confirming appropriate CVC placement, each method has inherent limitations. This case highlights the imperative need for a high index of suspicion to avert such complications and pretends to review some of each method's limitations.

Comparative Analysis of Impact Strength among Various Polymeric Materials for Orthotic Production.

Orthotic devices play an important role in medical treatment, addressing various pathologies and promoting patient recovery. Customization of orthoses to fit individual patient morphologies and needs is essential for optimal functionality and patient comfort. The advent of additive manufacturing has revolutionized the biomedical field, offering advantages such as cost reduction, increased personalization, and enhanced dimensional adaptability for orthotics manufacturing. This research focuses on the impact strength of nine polymeric materials printed by additive manufacturing, including an evaluation of the materials' performance under varying conditions comprising different printing directions (vertical and horizontal) and exposure to artificial sweat for different durations (0 days, 24 days, and 189 days). The results showed that Nylon 12 is good for short-term (24 days) immersion, with absorbed energies of 78 J and 64 J for the vertical and horizontal directions, whereas Polycarbonate (PC) is good for long-term immersion (189 days), with absorbed energies of 66 J and 78 J for the vertical and horizontal directions. Overall, the findings contribute to a better understanding of the suitability of these materials for biomedical applications, considering both short-term and long-term exposure to physiological and environmental conditions.

Mechanical Properties of Additively Manufactured Polymeric Materials-PLA and PETG-For Biomechanical Applications.

The study presented herein concerns the mechanical properties of two common polymers for potential biomedical applications, PLA and PETG, processed through fused filament fabrication (FFF)-Material Extrusion (ME). For the uniaxial tension tests carried out, two printing orientations-XY (Horizontal, H) and YZ (Vertical, V)-were considered according to the general principles for part positioning, coordinates, and orientation typically used in additive manufacturing (AM). In addition, six specimens were tested for each printing orientation and material, providing insights into mechanical properties such as Tensile Strength, Young's Modulus, and Ultimate Strain, suggesting the materials' potential for biomedical applications. The experimental results were then compared with correspondent mechanical properties obtained from the literature for other polymers like ASA, PC, PP, ULTEM 9085, Copolyester, and Nylon. Thereafter, fatigue resistance curves (S-N curves) for PLA and PETG, printed along 45°, were determined at room temperature for a load ratio, R, of 0.2. Scanning electron microscope observations revealed fibre arrangements, compression/adhesion between layers, and fracture zones, shedding light on the failure mechanisms involved in the fatigue crack propagation of such materials and giving design reference values for future applications. In addition, fractographic analyses of the fatigue fracture surfaces were carried out, as well as X-ray Computed Tomography (XCT) and Thermogravimetric (TGA)/Differential Scanning Calorimetric (DSC) tests.

Jumping Interval Training: An Effective Training Method for Enhancing Anaerobic, Aerobic, and Jumping Performances in Aerobic Gymnastics.

The aim of this study was to compare the effects of jumping interval training (JIT) and running high-intensity interval training (HIIT) on the aerobic, anaerobic and jumping performances of youth female aerobic gymnasts. A randomized controlled study was conducted over an 8-week period, involving 73 youth female athletes (16.2 ± 1.3 years old) of aerobic gymnastics. The study comprised two experimental groups (JIT and HIIT) and a control group. Participants in the experimental groups engaged in two additional training sessions per week alongside their regular training regimen, while the control group followed their usual training routine. Before and after the intervention period, gymnasts were assessed for their performance in the countermovement jump test (CMJ), the specific aerobic gymnastics anaerobic test (SAGAT) and the 20-m multistage fitness test. Significant interactions time × group were found in SAGAT (p < 0.001; = 0.495), CMJ (p < 0.001; = 0.338) and 20-m multistage fitness test (p < 0.001; = 0.500). The time × group analysis post-intervention revealed significantly lower scores in SAGAT for the control group compared to the JIT (p = 0.003) and HIIT (p = 0.034). Additionally, significantly higher scores were observed for the JIT group in the CMJ test compared to the HIIT (p = 0.020) and control (p = 0.028) groups following the intervention. Finally, the 20 m multistage fitness test post-intervention revealed significantly lower scores for the control group compared to JIT (p < 0.001) and HIIT (p < 0.001). Both JIT and HIIT are recommended training strategies to adopt in aerobic gymnastics for significantly improving the aerobic and anaerobic performances of athletes. However, JIT may be particularly relevant to use as it offers additional benefits in improving vertical jumping performances.

Sleep, anxiety, depression, and stress in critically ill patients: a descriptive study in a Portuguese intensive care unit.

BACKGROUND: Sleep disorders are common among patients admitted to intensive care units (ICUs). This study aimed to assess the perceptions of sleep quality, anxiety, depression, and stress reported by ICU patients and the relationships between these perceptions and patient variables. METHODS: This cross-sectional study used consecutive non-probabilistic sampling to select participants. All patients admitted for more than 72 hours of ICU hospitalization at a Portuguese hospital between March and June 2020 were asked to complete the "Richard Campbell Sleep Questionnaire" and "Anxiety, depression, and Stress Assessment Questionnaire." The resulting data were analyzed using descriptive statistics, Pearson's correlation coefficient, Student t-tests for independent samples, and analysis of variance. The significance level for rejecting the null hypothesis was set to α ≤0.05. RESULTS: A total of 52 patients admitted to the ICU for at least 72 hours was recruited. The mean age of the participants was 64 years (standard deviation, 14.6); 32 (61.5%) of the participants were male. Approximately 19% had psychiatric disorders. The prevalence of self-reported poor sleep was higher in women (t[50]=2,147, P=0.037) and in participants with psychiatric problems, although this difference was not statistically significant (t[50]=-0.777, P=0.441). Those who reported having sleep disorders before hospitalization had a worse perception of their sleep. CONCLUSIONS: Sleep quality perception was worse in female ICU patients, those with psychiatric disorders, and those with sleep alterations before hospitalization. Implementing early interventions and designing nonpharmacological techniques to improve sleep quality of ICU patients is essential.

Development of the Low-Pressure Die Casting Process for an Aluminium Alloy Part.

The low-pressure die casting (LPDC) process was experimentally and numerically studied to produce AlSi7Mg0.3 components such as steering knuckles. Steering knuckles are important safety components in the context of a vehicle's suspension system, serving as the mechanical interface that facilitates the articulation of the steering to control the front wheel's orientation, while simultaneously bearing the vertical load imposed by the vehicle's weight. This work focuses on the development of a numerical model in ProCAST®, replicating the production of the aforementioned part. The model analyses parameters such as the filling dynamics, solidification process, and presence of shrinkage porosities. For the purpose of evaluating the quality of the castings, six parts were produced and characterised, both mechanically (tensile and hardness tests) and microstructurally (porosity and optical microscopy analysis). When correlating simulation results with the available experimental data, it is possible to conclude that the usage of the LPDC process is a viable alternative to the use of steels and other metals for the production of very high-quality castings while using lighter alloys such as aluminium and magnesium in more demanding applications.

Relationships among Physical Fitness, External Loads, and Heart Rate Recovery: A Study on Futsal Players during an Overseas Congested-Weeks Training Camp.

This study examined relationships among players' physical characteristics, match external loads, and heart rate recovery (HRR) during match substitutions in a congested fixture of an overseas futsal training camp. Eleven under-20 national futsal players' anthropometric characteristics (age, body height, body mass, % fat, and % muscle) and physical fitness [HRmax, VO2max, maximal aerobic speed (MAS) during the 30-15 intermittent fitness test (IFT)] were determined. Additionally, locomotion profiles during field play and HRR sitting on the bench were recorded during five matches. A repeated-measures analysis of variance and Pearson's correlation coefficient were used for statistical analysis. The results revealed that the overall observed correlations among anthropometry, body composition, physical fitness, and HRR were inconsistent across all the matches and substitutions. However, the numbers of moderate (1.00-1.99 m/s2), moderate-to-high (2.00-2.99 m/s2), and high (3.00-50.00 m/s2) intensities of acceleration presented negative correlations in the last match (r < -0.76; p < 0.05). HRR during match substitutions may have been influenced by uncontrolled factors across all the match play and recovery. HRR measures may be affected mainly by fatigue caused by the accumulation of accelerations throughout a congested fixture during a congested-schedule of a futsal training camp.

Light-wave-controlled Haldane model in monolayer hexagonal boron nitride.

In recent years, the stacking and twisting of atom-thin structures with matching crystal symmetry has provided a unique way to create new superlattice structures in which new properties emerge1,2. In parallel, control over the temporal characteristics of strong light fields has allowed researchers to manipulate coherent electron transport in such atom-thin structures on sublaser-cycle timescales3,4. Here we demonstrate a tailored light-wave-driven analogue to twisted layer stacking. Tailoring the spatial symmetry of the light waveform to that of the lattice of a hexagonal boron nitride monolayer and then twisting this waveform result in optical control of time-reversal symmetry breaking5 and the realization of the topological Haldane model6 in a laser-dressed two-dimensional insulating crystal. Further, the parameters of the effective Haldane-type Hamiltonian can be controlled by rotating the light waveform, thus enabling ultrafast switching between band structure configurations and allowing unprecedented control over the magnitude, location and curvature of the bandgap. This results in an asymmetric population between complementary quantum valleys that leads to a measurable valley Hall current7, which can be detected by optical harmonic polarimetry. The universality and robustness of our scheme paves the way to valley-selective bandgap engineering on the fly and unlocks the possibility of creating few-femtosecond switches with quantum degrees of freedom.

The socioeconomic epidemiology of inherited retinal diseases in Portugal.

BACKGROUND: Inherited retinal diseases (IRDs) are a group of rare degenerative disorders of the retina that can lead to blindness from birth to late middle age. Knowing the target population and its resources is essential to better plan support measures. The aim of this study was to evaluate the socioeconomic characteristics of regions in Portugal where IRD patients reside to inform the planning of vision aid and rehabilitation intervention measures. RESULTS: This study included 1082 patients from 973 families, aged 3 to 92 years, with a mean age of 44.8 ± 18.1 years. Patients living with an IRD were identified in 190 of the 308 municipalities. According to this study, the estimated IRD prevalence in Portugal was 10.4 per 100,000 inhabitants, and by municipalities, it ranged from 0 to 131.2 per 100,000 inhabitants. Overall, regions with a higher prevalence of IRD have a lower population density (r=-0.371, p < 0.001), a higher illiteracy rate (r = 0.404, p < 0.001) and an overall older population (r = 0.475, p < 0.001). Additionally, there is a lower proportion of doctor per capita (r = 0.350, p < 0.001), higher social security pensions beneficiaries (r = 0.439, p < 0.001), worse water quality for human consumption (r=-0.194, p = 0.008), fewer audiences at the cinema (r=-0.315, p < 0.001) and lower proportion of foreign guests in tourist accommodations (r=-0.287, p < 0.001). CONCLUSION: The number of identified patients with IRD varied between regions. Using data from national statistics (PORDATA), we observed differences in socioeconomic characteristics between regions. Multiple targeted aid strategies can be developed to ensure that all IRD patients are granted full clinical and socioeconomic support.

Valleytronics in bulk MoS2 with a topologic optical field.

The valley degree of freedom1-4 of electrons in materials promises routes towards energy-efficient information storage with enticing prospects for quantum information processing5-7. Current challenges in utilizing valley polarization are symmetry conditions that require monolayer structures8,9 or specific material engineering10-13, non-resonant optical control to avoid energy dissipation and the ability to switch valley polarization at optical speed. We demonstrate all-optical and non-resonant control over valley polarization using bulk MoS2, a centrosymmetric material without Berry curvature at the valleys. Our universal method utilizes spin angular momentum-shaped trefoil optical control pulses14,15 to switch the material's electronic topology and induce valley polarization by transiently breaking time and space inversion symmetry16 through a simple phase rotation. We confirm valley polarization through the transient generation of the second harmonic of a non-collinear optical probe pulse, depending on the trefoil phase rotation. The investigation shows that direct optical control over the valley degree of freedom is not limited to monolayer structures. Indeed, such control is possible for systems with an arbitrary number of layers and for bulk materials. Non-resonant valley control is universal and, at optical speeds, unlocks the possibility of engineering efficient multimaterial valleytronic devices operating on quantum coherent timescales.

Innovative Design and Development of Personalized Ankle-Foot Orthoses for Survivors of Stroke With Equinovarus Foot: Protocol for a Feasibility and Comparative Trial.

BACKGROUND: Ankle-foot orthoses (AFOs) are vital in gait rehabilitation for patients with stroke. However, many conventional AFO designs may not offer the required precision for optimized patient outcomes. With the advent of 3D scanning and printing technology, there is potential for more individualized AFO solutions, aiming to enhance the rehabilitative process. OBJECTIVE: This nonrandomized trial seeks to introduce and validate a novel system for AFO design tailored to patients with stroke. By leveraging the capabilities of 3D scanning and bespoke software solutions, the aim is to produce orthoses that might surpass conventional designs in terms of biomechanical effectiveness and patient satisfaction. METHODS: A distinctive 3D scanner, complemented by specialized software, will be developed to accurately capture the biomechanical data of leg movements during gait in patients with stroke. The acquired data will subsequently guide the creation of patient-specific AFO designs. These personalized orthoses will be provided to participants, and their efficacy will be compared with traditional AFO models. The qualitative dimensions of this experience will be evaluated using the Quebec User Evaluation of Satisfaction With Assistive Technology (QUEST) assessment tool. Feedback from health care professionals and the participants will be considered throughout the trial to ensure a rounded understanding of the system's implications. RESULTS: Spatial-temporal parameters will be statistically compared using paired t tests to determine significant differences between walking with the personalized orthosis, the existing orthosis, and barefoot conditions. Significant differences will be identified based on P values, with P<.05 indicating statistical significance. The Statistical Parametric Mapping method will be applied to graphically compare kinematic and kinetic data across the entire gait cycle. QUEST responses will undergo statistical analysis to evaluate patient satisfaction, with scores ranging from 1 (not satisfied) to 5 (very satisfied). Satisfaction scores will be presented as mean and SD values. Significant variations in satisfaction levels between the personalized and existing orthosis will be assessed using a Wilcoxon signed rank test. The anticipation is that the AFOs crafted through this innovative system will either match or outperform existing orthoses in use, with higher patient satisfaction rates. CONCLUSIONS: Embracing the synergy of technology and biomechanics may hold the key to revolutionizing orthotic design, with the potential to set new standards in patient-centered orthotic solutions. However, as with all innovations, a balanced approach, considering both the technological possibilities and individual patient needs, will be paramount to achieving optimal outcomes. INTERNATIONAL REGISTERED REPORT IDENTIFIER (IRRID): PRR1-10.2196/52365.

PERK inhibition in zebrafish mimics human Wolcott-Rallison syndrome phenotypes.

Wolcott-Rallison Syndrome (WRS) is the most common cause of permanent neonatal diabetes mellitus among consanguineous families. The diabetes associated with WRS is non-autoimmune, insulin-requiring and associated with skeletal dysplasia and growth retardation. The therapeutic options for WRS patients rely on permanent insulin pumping or on invasive transplants of liver and pancreas. WRS has a well identified genetic cause: loss-of-function mutations in the gene coding for an endoplasmic reticulum kinase named PERK (protein kinase R-like ER kinase). Currently, WRS research is facilitated by cellular and rodent models with PERK ablation. While these models have unique strengths, cellular models incompletely replicate the organ/system-level complexity of WRS, and rodents have limited scalability for efficiently screening potential therapeutics. To address these challenges, we developed a new in vivo model of WRS by pharmacologically inhibiting PERK in zebrafish. This small vertebrate displays high fecundity, rapid development of organ systems and is amenable to highly efficient in vivo drug testing. PERK inhibition in zebrafish produced typical WRS phenotypes such as glucose dysregulation, skeletal defects, and impaired development. PERK inhibition in zebrafish also produced broad-spectrum WRS phenotypes such as impaired neuromuscular function, compromised cardiac function and muscular integrity. These results show that zebrafish holds potential as a versatile model to study WRS mechanisms and contribute to the identification of promising therapeutic options for WRS.