RESUMO
Dopamine (DA) is a key regulator of circuits controlling movement and motivation. A subset of midbrain DA neurons has been shown to express the vesicular glutamate transporter (VGLUT)2, underlying their capacity for glutamate release. Glutamate release is found mainly by DA neurons of the ventral tegmental area (VTA) and can be detected at terminals contacting ventral, but not dorsal, striatal neurons, suggesting the possibility that target-derived signals regulate the neurotransmitter phenotype of DA neurons. Whether glutamate can be released from the same terminals that release DA or from a special subset of axon terminals is unclear. Here, we provide in vitro and in vivo data supporting the hypothesis that DA and glutamate-releasing terminals in mice are mostly segregated and that striatal neurons regulate the cophenotype of midbrain DA neurons and the segregation of release sites. Our work unveils a fundamental feature of dual neurotransmission and plasticity of the DA system.-Fortin, G. M., Ducrot, C., Giguère, N., Kouwenhoven, W. M., Bourque, M.-J., Pacelli, C., Varaschin, R. K., Brill, M., Singh, S., Wiseman, P. W., Trudeau, L.-E. Segregation of dopamine and glutamate release sites in dopamine neuron axons: regulation by striatal target cells.
Assuntos
Corpo Estriado/metabolismo , Dopamina/metabolismo , Neurônios Dopaminérgicos/metabolismo , Ácido Glutâmico/metabolismo , Transmissão Sináptica , Área Tegmentar Ventral/metabolismo , Proteína Vesicular 2 de Transporte de Glutamato/fisiologia , Animais , Corpo Estriado/citologia , Neurônios Dopaminérgicos/citologia , Masculino , Camundongos , Camundongos Knockout , Área Tegmentar Ventral/citologiaRESUMO
The first synthesis of a chelating and reactive surfactant derived from citric acid and a short silicone as hydrophobic tail is described. Aqueous solutions of this reactive amphiphile spontaneously induce gold ion reduction, particle nucleation, and further direct crystal growth. The process, both pH and light dependent, occurs through lipid-directed assembly of metal ions, their reduction and subsequent lipid-directed growth to yield ultrathin (approximately 7 nm thick) quasi two-dimensional gold nanocrystals.
Assuntos
Materiais Biomiméticos/síntese química , Elétrons , Ouro/química , Nanopartículas Metálicas/química , Nanopartículas Metálicas/ultraestrutura , Materiais Biomiméticos/química , Ácido Cítrico/química , Cristalização , Microscopia Eletrônica de Varredura , Microscopia Eletrônica de Transmissão , Estrutura Molecular , Espectrofotometria , Tensoativos/síntese química , Tensoativos/químicaRESUMO
Exposure of a clean gold surface to tetrahydrofuran (THF) under ambient conditions was observed to cause roughening of atomic step edges. This change was followed in situ using a scanning tunneling microscope during the exposure of a gold surface to a controlled stream of THF vapor. THF is a common solvent used in depositing molecules, self-assembled monolayers, and polymer films on surfaces, in electrochemistry, and in chemical reactions. Unlike other solvents, such as methanol, ethanol and diethyl ether, however, we found that THF itself has a profound effect on the surface morphology that needs to be taken into account when reporting on the interactions of solutes with a gold surface. At the same time, this finding may present new opportunities in catalysis or nanostructuring of surfaces.
Assuntos
Furanos/química , Ouro/química , Propriedades de SuperfícieRESUMO
Nanoscale surface patterning is of great importance for applications ranging from catalysts to biomaterials. We show the formation of ordered nanoscale dimple arrays on titanium, tungsten, and zirconium during electropolishing, demonstrating versatility of a process previously only reported for tantalum. This is a rare example of an electrochemical pattern formation process that can be translated to other materials. The dimpled surfaces have been characterized with scanning electron microscopy, transmission electron microscopy, atomic force microscopy, and X-ray photoelectron spectroscopy, and electrochemical conditions were optimized for each material. While conditions for titanium and tungsten resemble those for tantalum, zirconium requires a different type of electrolyte. Given the appropriate electropolishing chemistry, formation of these patterns should be possible on any metal surface. The process is very robust on homogeneous surfaces, but sensitive to inhomogeneities in chemical composition, such as in the case of differentially etched alloys. An alternative process for some materials such as platinum is the coating of a dimpled substrate with a thin film of the required material.
Assuntos
Eletroquímica , Nanotecnologia/métodos , Elementos de Transição/química , Microscopia de Força Atômica , Microscopia Eletrônica de Varredura , Propriedades de SuperfícieRESUMO
Ultrathin films are important nanoscale structures that are used extensively in a variety of technological contexts. However, it has traditionally been difficult and costly to fabricate detachable and purely inorganic high aspect ratio films with controlled thickness and good uniformity. Here we report a versatile method to make separable purely inorganic membranes of various metal oxides such as Nb(2)O(5), TiO(2), WO(3), and Ta(2)O(5) with thicknesses ranging from 30 to 150 nm. Fluoride ions are migrated through the oxide film and upon arrival at the oxide-metal interface form a sacrificial soluble oxyfluoride layer. Fluorine also plays a role in controlling the porosity of the films. The study exposes the mechanism behind the detachment process that is largely due to the fast migration of fluoride anions relative to oxygen anions. The resulting films have a wide range of potential applications as catalysts or catalyst supports, filtration membranes, sensors, and more.
Assuntos
Metais/química , Nanotecnologia/métodos , Óxidos/química , Catálise , Eletroquímica/métodos , Eletrólitos , Fluoretos/química , Íons , Microscopia de Força Atômica , Microscopia Eletrônica de Varredura , Microscopia Eletrônica de Transmissão , Modelos Químicos , Polímeros/química , Porosidade , Propriedades de SuperfícieRESUMO
We report a simple electrochemical method of making individual free-standing and uniform tantalum oxide membranes between 35 and 100 nm thick. These films can be separated, floated on water, and transferred onto various substrates such as Si wafers, glass slides, and TEM grids. Our membranes are mechanically, chemically, and thermally robust, have a high dielectric constant, and a high refractive index, making them potentially useful in sensors, optics, filtration, and catalysis.
Assuntos
Cristalização/métodos , Eletroquímica/métodos , Compostos Inorgânicos/química , Membranas Artificiais , Nanoestruturas/química , Nanoestruturas/ultraestrutura , Óxidos/química , Tantálio/química , Adsorção , Substâncias Macromoleculares/química , Teste de Materiais , Conformação Molecular , Nanotecnologia/métodos , Tamanho da Partícula , Refratometria , Propriedades de SuperfícieRESUMO
We show that electropolishing of tantalum metal in a single step of about 5 min can reproducibly lead to dimples tens of nanometers in diameter, regular in shape, monodispersed in size, and arranged in highly ordered arrays which even transverse grain boundaries. Dimpled tantalum is ductile, high melting, and chemically inert, which makes it suitable for nanostructure synthesis even under extreme conditions, as demonstrated with a simple sputter coating and flame annealing procedure for gold nanoparticles.