RESUMO
UNLABELLED: OBJECTIVES. Chromosomal aberrations (CAs) are the one of the most sensitive biomarikers of biological effects from the hazardous environmental exposure. In this relation the comparison of cytogenetical indices in persons, exposed to the complex of factors of chemically hazard enterprises, with the presence of occupationally caused diseases seems to be very perspective for the understanding of the role of the contribution of genotoxical impact of occupational factors into staff morbidity. METHODS. Cytogenetical analysis was performed in 138 employees of chemically hazardous enterprises, including 84 patients, who need for establishment of the causation of diseases with the work in conditions of chemical plant and 54 persons without need for hospitalization according to results of the previous examination. Comparison group was consisted of 55 persons. There was performed the comparison of cytogenetical indices and commensuration of ratios of persons with individual high CAs levels and also carriers of exchange aberrations of chromosomal type in different groups of examined cases. RESULTS. There was shown statistically significant increase of the rate of CAs level including unexpectedly high level of dicentric and ring chromosomes in the group of 84 hospitalized patients. There was revealed statistically significant gain in the share of persons with CAs rate more then 5% (p < 0.01) and/or carriers of dicentrics and ring chronosomes (p < 0.5) in this group. CONCLUSION: It is possible to suggest that genotoxical impact plays a significant role in the mechanism of appearance of diseases etiologically related with the work on the enterprise of the high chemical risk.
Assuntos
Transtornos Cromossômicos/epidemiologia , Substâncias Perigosas/efeitos adversos , Nível de Saúde , Doenças Profissionais/epidemiologia , Exposição Ocupacional/efeitos adversos , Transtornos Cromossômicos/induzido quimicamente , Transtornos Cromossômicos/diagnóstico , Análise Citogenética , Feminino , Humanos , Incidência , Masculino , Doenças Profissionais/induzido quimicamente , Doenças Profissionais/diagnóstico , Federação Russa/epidemiologiaRESUMO
In organotypic tissue culture, the effect of the 20 coded amino acids combinations, in the effective concentration of 0.05 ng/ml, on the cell proliferation development in the spleen, myocardium, pancreas and brain cortex explants in 24 months rats was investigated. The combinations of two amino acids (stimulating and inhibiting proliferation) produced in the tissue culture the stimulating effect, exceeding by 8-10% as compared to the isolating effect of the stimulating amino acid. The results obtained form the base for the further synthesis of new effective dipeptides to be used to increase the regeneration of tissues when aging.
Assuntos
Aminoácidos , Apoptose/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Fatores Etários , Aminoácidos/farmacocinética , Aminoácidos/farmacologia , Animais , Encéfalo , Linhagem Celular , Fígado , Miocárdio , Pâncreas , Ratos , Ratos Wistar , Transdução de Sinais , Técnicas de Cultura de Tecidos/métodosRESUMO
The article presents the results of a comprehensive staff survey of the objects of storage and destruction of toxic chemicals. It was established that the structure of morbidity predominated respiratory diseases, cardiovascular system, nervous system and gastrointestinal tract. There are features of development and a current of some somatic diseases.
Assuntos
Doenças Cardiovasculares/diagnóstico , Substâncias para a Guerra Química/efeitos adversos , Gastroenteropatias/diagnóstico , Militares , Doenças do Sistema Nervoso/diagnóstico , Exposição Ocupacional/efeitos adversos , Doenças Respiratórias/diagnóstico , Adulto , Doenças Cardiovasculares/induzido quimicamente , Doenças Cardiovasculares/patologia , Doenças Cardiovasculares/terapia , Gastroenteropatias/induzido quimicamente , Gastroenteropatias/patologia , Gastroenteropatias/terapia , Humanos , Masculino , Pessoa de Meia-Idade , Doenças do Sistema Nervoso/induzido quimicamente , Doenças do Sistema Nervoso/patologia , Doenças do Sistema Nervoso/terapia , Doenças Respiratórias/induzido quimicamente , Doenças Respiratórias/patologia , Doenças Respiratórias/terapiaRESUMO
Cytogenetical examination was made in 49 workers of higher chemical hazard enterprises and 26 dwellers from industrial communities, who were engaged in enterprise security. Analysis of chromosome aberrations (CA) in the examined groups revealed a 1.8-fold increase in total CA rates in the industrial community dwellers securing the enterprise as compared to the controls mainly due to the rise in the rate of single fragments. At the same time in the enterprise workers, the total level of CA was 2.36- and 4.1-fold greater than that in the population of industrial communities and in the controls, respectively; and it was also characterized by a broad spectrum of CA with the increased number of both chromatid and chromosomal exchange aberrations.