Effect of aerobic exercise on anthropometric parameters among Indian primary school children.

Childhood obesity is a global public health concern with significant implications for long-term health. This study addresses the rising rates of obesity among school-age children (10-12 years) and investigates the effectiveness of aerobic exercise interventions in improving anthropometric parameters, specifically focusing on BMI and mid-arm circumference (MAC). The study emphasizes the role of schools in shaping children's behaviors and aims to contribute empirical evidence to inform health promotion strategies for this demographic. The research employs a quasi-experimental design, involving 60 school-age children in Visnagar, Gujarat, India. The 12-week aerobic exercise intervention, conducted three times a week, comprises activities like running, jumping jacks, and dance routines. Data collection includes sociodemographic information, BMI, and MAC measurements. The study design, participant criteria, and intervention details are carefully outlined. Socio-demographic variables such as age and monthly family income significantly influence BMI, highlighting the importance of considering these factors in interventions. Pretest results show 80% of children classified as overweight, reducing to 58.3% post-intervention. The mean BMI significantly decreases from 24.41 to 22.84 (p < 0.05), indicating the positive impact of aerobic exercise. The study also explores the association between BMI, MAC, and socio-demographic variables through chi-square tests. Data shows the prevalence of overweight and obesity among school-age children and demonstrates the effectiveness of a 12-week aerobic exercise program in improving BMI. Findings align with existing literature on the positive impact of physical activity on weight management in children.

Deep breathing exercises in easing educational stress among Indian high school students.

In this educational landscape, the pervasive issue of stress among higher secondary students has emerged as a serious matter. Acknowledging the challenges posed by educational stress, this research explores holistic and accessible interventions, with a focus on the promising avenue of deep breathing exercises known for their effectiveness in promoting relaxation and reducing stress. The study employs a quasi-experimental design, comparing an experimental group engaged in daily deep breathing exercises with a control group following a regular routine. Sixty higher secondary students in Visnagar participate through purposive sampling, adhering to specific inclusion criteria. The intervention includes a pre-tested questionnaire to assess stress levels, the implementation of daily deep breathing exercises in the experimental group, and a post-intervention stress level reassessment in both groups. The findings reveal a notable reduction in stress levels post-intervention, particularly in the experimental group practicing deep breathing exercises. Statistically significant reductions in mean stress scores underscore the effectiveness of this intervention, with the experimental group demonstrating a significantly lower mean stress score compared to the control group. This study contributes vital insights into stress management strategies for higher secondary students, highlighting the efficacy of incorporating deep breathing exercises into their routine. The observed reductions in stress levels emphasize the potential benefits of practical stress reduction techniques within the educational milieu.

Exploring eating behaviors, knowledge and attitudes of adolescent Indian girls.

Data on the eating behaviors, knowledge, and attitudes of adolescent girls in Visnagar, India, focusing on the prevalence of non-communicable diseases [NCDs] and their association with dietary practices is of interest. Adolescence, a crucial developmental phase, sets the foundation for lifelong health habits, necessitating an understanding of the determinants influencing eating behaviors. The research aims to identify gaps in knowledge, attitudes, and practices [KAP], providing insights for culturally sensitive public health strategies. Through structured questionnaires and Likert scales, data were collected from a purposive sample of adolescent girls [ages 12-18] in a selected school. Descriptive statistics and correlation analyses were employed to assess knowledge, attitudes, and behaviors, considering demographic variables. Non-significant associations were found between these variables and demographics. The mean knowledge score was 25.11, reflecting a moderate level, while the mean attitudes score was 99.54, indicating generally positive attitudes. Adolescent girls demonstrated an overall mean behaviour score of 110.93, with a positive correlation [0.72] between knowledge and behaviors and a stronger correlation [0.99] between attitudes and behaviors. Findings highlight the universal importance of knowledge in influencing eating behaviors and emphasize the need for culturally tailored interventions considering regional influences. The study contributes valuable insights into the interplay of knowledge, attitudes, and behaviors related to eating disorders in adolescent girls, serving as a foundation for targeted public health strategies.

Knowledge, attitudes and demographic effect on menopausal experiences among Indian rural women.

The menopausal experiences of women in selected rural areas of Visnagar, addressing a research gap within cultural and socio-economic landscapes is of interest. The global aging trend was reflected in the 26% representation of women aged 50 and over in the study. Employing a cross-sectional design, 200 menopausal women were sampled through stratified random sampling, emphasizing demographic diversity. Results from a structured Knowledge Questionnaire and a Likert Scale for Attitude revealed that women generally possessed commendable knowledge (mean score 10.94) and positive attitudes (mean score 28.66) toward menopause. Significant associations between age, education, occupation, and income with knowledge and attitudes were uncovered through chi-square tests. This study underscored the need for tailored interventions in rural settings, considering the influence of cultural, regional, and demographic factors on menopausal experiences. The findings contributed to bridging the research gap and emphasized the importance of individualized approaches for promoting the well-being of menopausal women in rural communities.

Impact of oil massage on newborn behavioural responses in rural India.

The initial weeks of a newborn's life are marked by rapid physiological and behavioural adjustments as the infant adapts to the external environment. This critical period necessitates attentive care, prompting exploration into traditional practices such as oil massage, which holds cultural significance and is believed to enhance neonatal well-being. Despite its prevalence, empirical evidence supporting the efficacy of oil massage remains limited. This study, conducted in a rural setting, aims to bridge traditional practices with evidence-based care, exploring the impact of oil massage on newborn behavioural responses. A quasi-experimental design involving 60 newborns (30 in each group) assessed behavioural responses through a pre and post-test approach. Results indicate a significant improvement in selected behavioural responses among newborns receiving oil massage, emphasizing its potential integration into routine care. The control group showed a pre-test mean of 14.83 (SD = 2.41) and a post-test mean of 16.23, while the experimental group exhibited a pre-test mean of 15.83 (SD = 1.80) and a post-test mean of 26.07. T-test values of 5.194 for the control group and 26.137 for the experimental group were indicative of statistically significant changes. The study contributes insights into neonatal care practices, urging further exploration of contextual intricacies and demographic influences on newborn behaviour.

Breast feeding practices after normal vaginal and caesarean delivery in Gujarat, India.

Breast feeding is the mainspring of child survival, nutrition, development and maternal health. Early initiation of breastfeeding is an extremely important factor associated with the maintenance of long-term breastfeeding practice. Breastfeeding practices can be influenced by a variety of variables such as parity, mode of delivery; body mass index (BMI), breast or nipple abnormalities and behavioural factors are equally as important. The present study was conducted to analyze Breast Feeding Practices after Normal Delivery and Caesarean Delivery at a Tertiary Care Hospital. This cross sectional study was conducted at tertiary care hospital, by Convenient non-random sampling method, which included two groups; Group A: 100 mothers who delivered vaginally Group B: 100 mothers who gave birth through caesarean section (n=100 each). Participants were asked to complete standardized questionnaire consists of information on socio demographic and breast feeding practice. All anthropometric measurements were taken. A semi-structured questionnaire was used to collect data on maternal socio demographic characteristics, breastfeeding knowledge, practices along with source of information regarding breastfeeding and maternal experience. The study results shows that initiation of breastfeeding is most common in normal vaginal delivery (70%) among total 100 subjects of vaginal delivery category and also common in subjects with planned C-section (49%) of 100 subject"s caesarean delivery category. Association between the modes of delivery and initiation of breastfeeding within an hour was statistically significant (p<0.01). The present study indicates that C-sections are linked to higher breastfeeding challenges, greater resource usage, and shorter nursing duration.

Effect of curry leaves in lowering blood pressure among hypertensive Indian patients.

Hypertension, commonly known as high or rising blood pressure, is characterized by a consistently elevated blood arterial pressure. It is a significant medical condition called hypertension raises your risk of developing problems with your heart, brain, kidneys, and other organs. Curry leaves work well as a treatment for high blood pressure. Curry leaves are thought to have a potent therapeutic impact for the treatment of high blood pressure since they are low in salt and high in potassium. Therefore, it is of interest to reduce the level of blood pressure among adults in rural area with the help of curry leaves. According to the statistical analysis, the experimental group's post-test mean score for hypertension was 155 with a standard deviation of 13.892 while the control group's post-test mean score was 162 with a standard deviation of 17.20. The estimated unpaired value of t = 11.07 was deemed to be statistically significant at the p 0.05 level and the mean difference was 7. As a result, the null hypothesis was rejected and the research hypothesis was accepted. Giving Curry leaf powder to patients with hypertension as a consequence was an effective intervention.

Gene polymorphism among hypertensive patients with coronary artery disease.

The obstruction of the coronary arteries causes Coronary Artery Disease (CAD). It has been reported that interleukin-6 gene is related to the development of cardiovascular diseases such as atherosclerosis and coronary artery disease. This was due to the large variability and short half-life of interleukin 6 (IL-6). There are few studies on the link between interleukin 6 and CAD on the patients with hypertension. Therefore, goal of this study was to see if there is a link between IL-6 gene polymorphisms and coronary artery disease with hypertension patients. The polymorphisms were carried out by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The data was determined for statistical significance using chi-square analysis. A significant difference was found in the GG genotype of IL-6 -174, which was more frequent in cases of CAD (48.67 %) than in controls (8%) and 95% CI was 0.473455 - 0.500326; P<0.010620511. The GG genotype of IL-6-572C/G polymorphism was more frequent in cases of CAD (42.6%) compared with controls (8%) and 95% CI 0.386724 - 0.480945; P<0.017939631). likewise, significant association of variant allele G with CAD patients was reported. Hypertension was significantly higher among patients as compared to controls (P<0.022847535). Our findings indicated that both gene polymorphisms may be associated with development of CAD.

Effect of cotton dust on lungs among female workers in cotton industry in northern Gujarat, India.

Byssinosis is a disabling occupational lung disease caused by cotton dust. It is a well-known occupational respiratory disease in cotton industry workers caused by cotton dust pollution. Many studies have been documented the effects of cotton dust on pulmonary function among workers employed in cotton-spinning mills. However, little data exist on the prevalence of this disorder in female workers particularly in western part of India. The present study was conducted to analyze the effects of exposure to cotton dust on pulmonary functions among female workers. The study was designed to assess the effects of exposure to cotton dust on lung functions among female cotton industry workers. Study group comprises 50 Female workers of cotton industry and control group comprises 50 age matched females who were neither worked in cotton industry nor exposed to cotton dust. Information was collected using standardized questionnaires, physical examination and spirometric measurements. Student's T test was used to find the difference between spirometric parameters. All the respiratory parameters (FVC, FEV1, FEV1/FVC ratio, FEF 25-75 % PEFR and MVV) were reduced in cotton industry workers as compared with control subjects (p<0.0001) and no significant difference of SpO2 between groups. Cotton dust exposure makes huge impact on respiratory parameters of the cotton industry workers. This deterioration in respiratory health deteriorates with increasing duration of exposure. The health hazards caused by cotton dust should be controlled by creating awareness among the workers & employers.

Effect of Beta vulgaris extracts on dysmenorrhea among adolescent girls.

Dysmenorrhea is painful menstrual periods which are caused by uterine contractions. The ache is commonly felt in the pelvic or lower abdomen around the time menstruation begins. Periods aren't the best time for a woman to feel strong and energized. With all of the blood loss, cramps, and exhaustion, finding enough excitement to get through the day's responsibilities is difficult. Vulgaris Beta Potassium and nitrates, both of which are necessary for blood pressure regulation, are abundant in juice. For energy, only 50ml of beet juice is required. The analysis of data was done by descriptive and inferential statistics. The study identified that 46.66 % had moderate pain, 33.33 % had mild pain and none of them had severe pain for the pre- experimental group. The study result shows that the pre-test mean value for is 5.91 and pre-test SD 0.96. The post-test mean value is 2.86 and post-test SD is 1.04. The mean difference is 3.05. The calculated 't' value is 16.85 is higher than the table value 1.67. The study concluded that Beta vulgaris juice found to be effective non-pharmacological measures to reduce dysmenorrheal among adolescent girls.

Awareness on mosquito borne diseases among urban & rural population in Northern Gujarat.

It is of interest to examine the awareness and precautions of urban and rural residents on a number of mosquito-borne diseases (MBD). A sample of 300 adult people (Rural 150+Urban 150) was selected from Mahesana district of North Gujarat. Majority of samples (47.3 %) had average, 16% poor and 36.7% had good level of awareness in urban areas. In rural areas majority of the samples (40.67%) had average, 28%poor and 31.33% had good level of awareness. (67.3%) urban population were using mosquito repellent liquids and creams and (68.6%) of rural population were using mosquito net. Data shows that both urban and rural populations have moderate awareness on Mosquito Borne Diseases and majority is taking precaution towards these conditions. Data also revealed that there is no significant difference between urban & rural population's precaution measures against Mosquito borne diseases.

Effect of bibliotherapy on reducing anxiety among pre-operative patients in Gujarat, India.

Numerous studies have shown that patients can have preoperative anxiety in mild, moderate, or severe forms. A supplemental tool to a disease's clinical treatment is bibliotherapy. This approach includes the core ideas of cognitive behavioral therapy and offers exercises meant to assist readers in overcoming unpleasant emotions. Therefore, it is of interest to evaluate how well bibliotherapy reduced pre-operative patients' anxiety. For the experimental group (30) and control group (30), a sample of 60 preoperative patients who had been determined to have considerable levels of anxiety was chosen. The Hamilton Anxiety Rating Scale is used to measure patient anxiety. Prior to surgery, bibliotherapy was given to the experimental group's sample twice daily for around 20 minutes. No intervention was given to the control group. The study's findings showed that the experimental group's mean percentage anxiety score at the pre-test was 80.10 percent, whereas the control group's mean percentage anxiety score was 85.66 percent. After the test, the experimental group's mean anxiety score was 50.66 percent, while the control group's mean anxiety score was 83.20 percent. It is evident that bibliotherapy was successful in lowering pre-operative patients' anxiety levels. Nurses can utilize this non-pharmacological technique to help patients feel less anxious about surgery and experience fewer post-operative problems.

Effect of outdoor games among school children in Northern Gujarat, India.

Outdoor play works as an important tool for the children education. Providing a natural learning environment for children helps them to have an active and fulfilling life. Also, higher levels of attention and well-being are promoted when children play in green outdoor spaces. The importance of play for children's healthy development is grounded in a strong body of research. The study used an experimental research methodology, and data was obtained from 60 school-aged children using a purposive sample strategy and a checklist. The mean, standard deviation, and chi square test were used to analyze the data. After giving act out method, majority (85%) of the school age children had adequate knowledge, 15% of the school children had moderate knowledge regarding outdoor games and its importance. In data analysis the mean of pretest score was 6.43 and mean of post test score was 15.88. The mean difference was 9.45. Post test mean was more than pretest mean, which indicate the effectiveness of actout method on deficiency of outdoor game among school children. The standard deviation of pretest knowledge score was 3.9; the post test knowledge score was 2.47. The computed 't' value was 16.1, the DF was 59, and the P value was 1.67, all of which are significant. Religion, monthly income, and the age of the children all had an impact on the calculated chi square value. According to the findings of this study, the act out method was successful in boosting understanding about the lack of outdoor games among school-aged children.

Boosting awareness on healthy habits among school children in north Gujarat, India.

Hygiene is the science of health and its maintenance. Hygiene status of children is an index of national investment in the development of its man power. It is influenced by social, familial and individual factors as well as the children's knowledge of health on personal hygiene, comfort and basic needs. The utility of games is as a teaching strategy of health professionals. The main objectives of the study were to assess existing level of awareness regarding healthy habits among school children and to check the effect of Modified snake & ladder game in improving the awareness of children regarding healthy habits. Research design selected for this study was pre experimental one group pretest posttest design with a sample size of 60. Samples of the study were given opportunity to play and earn awareness through the modified snake & ladder game. Their awareness was assessed before and after the game section. Data was analyzed by using descriptive and inferential statistics such as mean, standard deviation and chi square test. In data analysis the mean of pre-test score was 13.83 and mean of post-test score was 18.63. The mean difference was 4.8. The standard deviation of pre-test stress score was 01.07 and post-test stress score was 01.60. The calculated' 't' value was 21.24, which was greater than the table value(1.67).The study concluded that snake and ladder game was effective in improving the awareness of school children on healthy habits.

Edge detection methods applied to the analysis of spherical raindrop images.

Optical imaging of raindrops provides important information on the statistical distribution of raindrop size and raindrop shape. These distributions are critical for extracting rainfall rates from both dual- and single-polarization radar signals. A large number of raindrop images are required to obtain these statistics, necessitating automatic processing of the imagery. The accuracy of the measured drop size depends critically on the characteristics of the digital image processing algorithm used to identify and size the drop. Additionally, the algorithm partially determines the effective depth of field of the camera/image processing system. Because a large number of drop images are required to obtain accurate statistics, a large depth of field is needed, which tends to increase errors in drop size measurement. This trade-off between accuracy and depth of field (dof) is also affected by the algorithm used to identify the drop outline. In this paper, eight edge detection algorithms are investigated and compared to determine which is best suited for accurately extracting the drop outline and measuring the diameter of an imaged raindrop while maintaining a relatively large depth of field. The algorithm which overall gave the largest dof along with the most accurate estimate of the size of the drop was the Hueckel algorithm [J. Assoc. Comput. Mach. 20, 634 (1973)].

Myocardial cytokine gene expression is higher in aortic stenosis than in idiopathic dilated cardiomyopathy.

OBJECTIVE: To investigate cytokine gene expression in patients with aortic valve stenosis (AS) and with idiopathic dilated cardiomyopathy (DCM), and to correlate wall stress with myocardial proinflammatory cytokine gene expression. METHODS: Human left ventricular (LV) myocardial biopsies were obtained for subsequent reverse transcription polymerase chain reaction of tumour necrosis factor alpha (TNFalpha), interleukin (IL)-1beta, and IL-6 mRNA. The study population consisted of 24 patients with AS and 10 patients with idiopathic DCM. RESULTS: Patients with AS had a larger ejection fraction (56 (5) v 37 (4)%, p < 0.01), smaller LV end diastolic volumes (146 (11) v 267 (21) ml, p < 0.01), and lower end systolic wall stress (44 (7) v 112 (11) kdyn/cm2, p < 0.001). Upregulation of TNFalpha, IL-1beta, and IL-6 gene expression was detected in both groups. However, TNFalpha gene expression was significantly higher in AS than in DCM (p = 0.009). No correlation was found between haemodynamic parameters and TNFalpha gene expression. In patients with AS there was a strong inverse relation between circulating TNFalpha and TNFalpha gene expression (r = -0.685, p = 0.014), between circulating TNFalpha and IL-1beta gene expression (r = -0.664, p = 0.018), and between soluble TNF receptor 2 and TNFalpha gene expression (r = -0.685, p = 0.020). Myocardial gene expression of TNFalpha was significantly higher in patients with well compensated AS than in patients with decompensated AS (p = 0.017). Similarly, patients with decompensated DCM were characterised by significantly lower TNFalpha gene expression than were patients with well compensated DCM (p = 0.011). CONCLUSION: TNFalpha gene expression is significantly higher in patients with pressure overload than in normal hearts, in patients with idiopathic DCM, and in patients with compensated versus decompensated heart failure. Secondly, in patients with AS proinflammatory cytokine gene expression did not affect systolic performance. The higher TNFalpha gene expression in patients with compensated heart failure suggests that cytokine gene expression has an adaptive role in the early phase of LV remodelling.

Differential regulation of mitogen-activated protein kinases in the failing human heart in response to mechanical unloading.

Background- Mechanical unloading of the heart with a left ventricular assist device (LVAD) leads to favorable changes in the biology of the failing cardiac myocyte. To determine a potential mechanism for these improvements, we examined the regulation of mitogen-activated protein kinases (MAPKs) in the failing heart in the presence and absence of LVAD support. Methods and Results- We examined the degree of activation (ie, phosphorylation) of p44/42 extracellularly regulated kinase, p38 kinase, and c-Jun N-terminal kinase (JNK1/2), and the corresponding activity levels of these MAPKs in myocardial samples obtained from 11 patients with LVAD support and in 11 patients without LVAD support. MAPK activity was also examined in an additional 6 patients from whom paired samples were obtained before and after LVAD support. The activity of p44/42 and JNK1/2 were reduced significantly, whereas p38 activity levels were significantly increased after LVAD support. We examined functional parameters that are linked to MAPK activation, namely cardiac myocyte hypertrophy and apoptosis. Both cardiac myocyte cell size and the incidence of cardiac myocyte apoptosis were significantly reduced after LVAD support. Conclusions- Mechanical unloading of the failing heart leads to differential regulation of MAPKs. These changes in MAPK activity are associated with changes in myocyte hypertrophy and viability, suggesting a potential mechanistic basis for some of the observed salutary changes after LVAD support.

Left ventricular remodeling in transgenic mice with cardiac restricted overexpression of tumor necrosis factor.

BACKGROUND: The mechanisms responsible for tumor necrosis factor (TNF)-induced LV structural remodeling in the adult heart are not known. METHODS AND RESULTS: We generated a line of transgenic mice (MHCsTNF) with cardiac restricted overexpression of TNF that develop progressive LV dilation/remodeling from 4 to 12 weeks of age. During the early phases of LV structural remodeling, there was a significant increase in total matrix metalloproteinase (MMP) activity that corresponded to a decrease in total myocardial fibrillar collagen content. As the MHCsTNF mice aged, there was a significant decrease in total MMP zymographic activity that was accompanied by an increase in total fibrillar collagen content. The changes in total MMP activity and myocardial fibrillar collagen content were related to a time- dependent increase in myocardial tissue inhibitor of metalloproteinases (TIMP)-1 levels, resulting in a significant time-dependent decrease in the MMP activity/TIMP level ratio in the MHCsTNF mice. To determine a possible mechanism for the increase in myocardial fibrosis, we also measured levels of TGF-beta(1) and TGF-beta(2) protein levels, which were shown to be significantly elevated in the hearts of the MHCsTNF mice. CONCLUSIONS: Our results suggest that progressive time-dependent changes in the balance between MMP activity and TIMP activity are responsible, at least in part, for the spectrum of TNF-induced changes in the myofibrillar collagen content that occur during LV structural remodeling in the MHCsTNF mice.

Homocysteine induces expression and secretion of monocyte chemoattractant protein-1 and interleukin-8 in human aortic endothelial cells: implications for vascular disease.

BACKGROUND: Proinflammatory cytokines play key roles in atherogenesis and disease progression. Because hyperhomocysteinemia is an independent risk factor for cardiovascular disease, we hypothesized that homocysteine could be atherogenic by altering the expression of specific cytokines in vascular endothelial cells. METHODS AND RESULTS: Northern blot and RNase protection assays showed that DL-homocysteine induced mRNA expression of the proinflammatory cytokines monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) in cultured human aortic endothelial cells (HAECs). Homocysteine had no effect on expression of other cytokines, namely tumor necrosis factor-alpha, granulocyte-macrophage colony-stimulating factor, interleukin-1beta, and transforming growth factor-beta. MCP-1 mRNA expression increased 1 hour after homocysteine treatment, reached a maximum within 2 to 4 hours, and declined to basal levels over the next 24 hours. Induction of mRNA expression for both chemokines was observed with as little as 10 micromol/L DL-homocysteine, and maximal expression was achieved with 50 micromol/L DL-homocysteine. Homocysteine also triggered the release of MCP-1 and IL-8 protein from HAECs into the culture medium. The induction was specific for homocysteine, because equimolar concentrations of L-homocystine, L-cysteine, and L-methionine had no effect on mRNA levels and protein release. Furthermore, L-homocysteine induced chemokine expression, but D-homocysteine did not, thus demonstrating enantiomeric specificity. The culture medium from homocysteine-treated HAECs promoted chemotaxis in human peripheral blood monocytes and U937 cells. Anti-human recombinant MCP-1 antibody blocked the migration. CONCLUSIONS: Pathophysiological levels of L-homocysteine alter endothelial cell function by upregulating MCP-1 and IL-8 expression and secretion. This suggests that L-homocysteine may contribute to the initiation and progression of vascular disease by promoting leukocyte recruitment.

In vivo expression of proinflammatory mediators in the adult heart after endotoxin administration: the role of toll-like receptor-4.

Tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, and nitric oxide (NO) may play a role in lipopolysaccharide (LPS)-induced cardiac depression. Toll-like receptor-4 (TLR-4) mediates the cytokine response to LPS in immune cells. TLR-4 also is expressed in human and murine myocardial tissue. Therefore, the hypothesis that LPS induces proinflammatory cytokines in the heart via TLR-4 was tested. C3H/HeJ (TLR-4 deficient) and C3HeB/FeJ mice were studied. LPS induced a robust increase in myocardial TNF-alpha and IL-1beta mRNA in C3HeB/FeJ mice. The response in C3H/HeJ mice was blunted and delayed. Myocardial TNF-alpha and IL-1beta protein levels were higher in C3HeB/FeJ mice, as were inducible NO synthase protein and NO production. Activation of myocardial NF-kappaB was observed within 30 min in C3HeB/FeJ mice but not in C3H/HeJ mice. These findings suggest that myocardial TLR-4 is involved in signaling cytokine production within the heart during endotoxic shock.