Modification of a Haematoxylin, Eosin, and Natural Saffron Staining Method for The Detection of Connective Tissue.

INTRODUCTION: The aim of our study was to optimise an existing staining procedure: haematoxylin-eosin saffron (HES). The method follows the classical haematoxylin and eosin protocol with the addition of a staining step using natural saffron to better identify the collagen fibres. MATERIAL AND METHODS: The saffron solution was obtained by dissolving ground saffron stigmas in absolute alcohol. In order to test the HES method for its staining ability on four main types of collagen (I, II, III, and IV), specific tissues (skin, tooth, cartilage, aorta, spleen, and penis) were chosen. RESULTS: The procedure showed a sharp differentiation between muscle, stained red or pink, and connective tissue, stained bright yellow or orange. CONCLUSION: HES allows the diagnosis of reticulin fibrosis undetected in HE and in previous saffron staining procedures. HES represents an advantageous alternative to HE staining giving highly reproducible results with high diagnostic value.

Histological and morphometric evaluation of the urethra and penis in male New Zealand White rabbits.

This stereudy aimed at performing a histological and morphometric evaluation of the urethra and penis of male rabbits. Seven male New Zealand White rabbits weighing 2.1-3 kg were used in the study. The whole urethra, from the urinary bladder to the external urethral orifice, was dissected from the rabbits, and the tissue was sliced into sections at an interval of 2 mm. The sections were stained with haematoxylin-eosin, Masson-Goldner trichrome stain, Van Gieson's stain and Movat-Russell modified pentachrome stain. A detailed evaluation of the morphology and morphometry was performed. The parameters assessed were the type and height of epithelium, thickness and composition of connective tissue, and thickness and structure of muscularis. The histological structure of the rabbit urethra was found to be similar to humans. However, although the rabbits were found to have the same type of penis as the humans, the internal structure of the corpora cavernosa, the relative thickness of the tunica albuginea and the rudimentary glands of the penis were found to differ in these animals. The results of the present study may be useful in the designing of implants, drug testing or surgical procedures on the physiological and pathological urethra.

The Influence of the Privatization Process on Accident Rates in the Forestry Sector in Poland.

The aim of this paper is to analyze the changes in accident rates resulting from the privatization of forest operations. Data from the years 1990-2017 were obtained from the Statistical Forestry Yearbooks issued by Statistics Poland, and were analyzed for two periods: the time of intensive privatization (1991-2002) and the post-privatization period (2003-2017). The data from 1990 were treated as a benchmark. There were 14,626 accidents in total, of which 236 (1.61%) were fatal. The non-fatal accident rate in the whole forestry industry showed a decreasing trend in the study period (t = 2.27, p < 0.05). In the case of the fatal accident rate we can observe an upward trend; in the period of intensive privatization the average annual fatality rate was 0.11, and after privatization it was 0.18 (t = -2.68, p < 0.05). In both periods the fatality rate was twice as high in the private forestry sector as in the public sector. The number of working days lost declined in the public sector and increased in the private sector. An accident in the private sector resulted in 20 days' longer absence than one in the public sector. The study confirms that despite economic transition, accident rates in Polish forestry remain a serious issue. The main problem to be addressed is the increase in the fatal accident rate, especially in the private sector.

Accessory Genital Glands in the New Zealand White Rabbit: A Morphometrical and Histological Study.

INTRODUCTION: The aim of this research was to provide a detailed description of the morphology, topography, and histometry of rabbit accessory genital glands. MATERIAL AND METHODS: Seven male New Zealand White rabbits, 3-4 months of age and weighing 2.1-3 kg were used for the study. The whole urethra from the urinary bladder to the external urethral orifice accompanied by accessory genital glands was sliced at intervals of 1 mm. The serial sections were prepared with haematoxylin-eosin (H&E) and Movat-Russell modified pentachrome stain. RESULTS: A detailed description of the morphology and morphometry was provided. The topography of the organs was explained on the basis of characteristic cross-sections on histological slides. The inconsistent nomenclature and descriptions of these glands by different authors were also discussed. CONCLUSION: The morphometric analysis indicated that some of the glands described have similar dimensions in different individuals, while others like paraprostates revealed high diversity in the number of lobes, their size, and their structure. The accessory glands are also good topographic markers which precisely define the segment of the urethra. The terms "proprostate", "prostate", and "paraprostates" as the nomenclature of the prostate complex reflect the location of these glands well and indicate their common origin and function.

Analysis of adolescents' opinions on suicide prevention. / Analiza opinii mlodziezy na temat profilaktyki samobójstw.

OBJECTIVES: The aim of the study is to investigate the views of adolescents aged 16-19, attending public high schools in Warsaw, on effectiveness of suicide prevention. METHODS: The studied population covered adolescents aged 16-19 back in 2015 (M = 17.29; SD = 0.94). The respondents attended eight public high schools in Warsaw in eight different districts. The study group was representative. 1,439 respondents participated in the study - 821 girls (57.1%) and 592 boys (41.1%). PAPI method was used in the conducted study. The questionnaire consisted of 34 questions. RESULTS: In most cases respondents did not know whether there was an organized system of suicide prevention in Poland (42.0%), however, a large proportion of the respondents (39.7%) was aware of nonexistence of such a system in Poland. The respondents considered the activity of a school (46.7%), the Church (38.3%) and the police (55.9%) in the field of suicide prevention ineffective, the activity of health care institutions (47.7%) and the media (46.6%) hardly effective, while the family was considered effective (70.9%). Persons who perceive significant role of the Church in suicide prevention at the same time indicate considerable share of the family and a much lesser role of the remaining institutions. CONCLUSIONS: 1) More than . of studied adolescents realize that there is no integrated system of suicide prevention; 2) The family, according to high school students, plays the greatest role in preventing self-destructive behaviors; 3) There is a need of greater involvement of schools, the Church, the police, health care institutions and the media in education and raising adolescents' awareness in the field of self-destructive behaviors.`.

Analysis of the opinions of adolescents on the risk factors of suicide. / Analiza opinii mlodziezy na temat czynników ryzyka popelnienia samobójstwa.

OBJECTIVES: The aim of this paper was to identify the opinions of adolescents aged 16-19, attending public high schools in Warsaw, on the risk factors of suicide. METHODS: The study group consisted of adolescents who were aged 16-19 in the year 2015 (M = 17.29; SD = 0.94). The respondents attended eight public high schools in Warsaw, from eight different districts of the city. The study group was representative. 1,439 respondents participated in the study -821 girls (57.1%) and 592 boys (41.1%). PAPI method was used in the conducted study. The questionnaire consisted of 34 questions divided into three sections - concerning epidemiology, risk factors and prevention of suicidal behaviors. RESULTS: According to 37.0% of respondents, the main problem occurring in a family that could become a risk factor in committing suicide is lack of understanding from the parents. The respondents indicated that conflicts between peers are school-related situations that could be the source of suicidal thoughts (48.6%). More than half of respondents (59.1%) believed that being rejected by close and important persons was the main factor related to the feeling of loneliness that could contribute to a suicidal act. CONCLUSIONS: 1) An important element in the prevention of self-destructive behaviors of young people should be parental training in understanding and accepting their children. 2) Itis advisable to conduct classes developing psycho-social skills of adolescents, in particular their ability to solve interpersonal problems, in order to reduce the number of conflicts with peers that occur in the school environment.

Risk assessment of night-eating syndrome occurrence in women in Poland, considering the obesity factor in particular.

INTRODUCTION: Night-eating syndrome (NES) involves uncontrolled and most often repeated binge eating during the night. It is related with mood disorders as well as sleep disorders and it may cause obesity. Risks related to NES are obesity, binge eating disorder, bulimia nervosa, affective disorders, and sleep disorders. The objective of this study is to analyze eating habits in terms of the risk assessment of NES occurrence in the population of women in the Masovian Voivodeship (in Poland). PATIENTS AND METHODS: Six hundred and eleven women living in the Masovian Voivodeship participated in the study. The average age of the respondents was 22.7 years (median = 23.0; interquartile range = 3.0). The Night Eating Questionnaire (NEQ) was used to assess the risk of NES. RESULTS: In the studied group of women, 1.3% of cases (N = 12) reached a NEQ total score of ≥25, which indicates a probability of 40.7% for NES, while 0.7% (N = 4) reached a score of ≥30, which indicates a probability of 72.2% for occurrence of this syndrome. The highest average total score was observed in the group of obese people. The level of education of the participants did not significantly affect the NEQ score. A weak correlation was observed between the place of residence variable and the mood/sleep subscale (r = 0.11, P < 0.01). CONCLUSION: NES may be one of the causes of overweight and obesity; therefore, the need for further studies on this health issue is justified. It is worth pointing out that knowing the conditions responsible for the occurrence of NES, it is possible to suggest a prevention procedure for this condition.

Assessment of knowledge about the effects of UV radiation on health behaviors associated with sunbathing in gymnasium students.

INTRODUCTION: The health behaviors or health-related behaviors is behavior (or activity) that are part of everyday life, affecting the health of the individual. An example of the behavior of health is also sunbathing, or exposing the body to excessive solar radiation dosage. It may be positive and negative effects on health. AIM: Evaluation of knowledge about gymnasium students. The impact of UV radiation on health and health behaviors associated with sunbathing. MATERIAL AND METHODS: The study was a diagnostic survey, with author's questionnaire. The sample was comprised students from classes II and III. A total of 312 questionnaires were collected among 181 girls and 131 boys. Used purposeful sampling. Results were considered statistically significant at p < 0.05. RESULTS: There is a statistically significant relationship between the stated sunbathing to get a sun tan, and sex of the respondent (p = 0.0002). Definitely more girls (77.35%) admit that tans in the sun to get a tan as compared to boys (58.02%). CONCLUSION: It is recommended that further research aimed at checking the causes and incidence of sunburn among young people. Consideration should be given to create and implement the appropriate health programs taking about tanning that could be implemented under the School Health Promotion Program, or to supplement this knowledge on subjects such as Biology or Nature.