RESUMO
Mutation Y168H of the human phenylalanine hydroxylase (PAH) gene determining phenylketonuria was described only twice: in a patient from Catalonia (Spain) and by us in a patient from Western Siberia (Russia). The association of Y168H in these families with allelic variants of STR and VNTR repeats and a number of neutral point polymorphisms of the PHA gene (IVS3nt-22C > T, Q232Q, V245V, L385L) was studied in this work. The Y186H mutation in these families was found to be associated with different haplotypes. Strong linkage of the selected markers and the mutation region excludes recombination as a possible cause of association of Y168H with different haplotypes. It was concluded that Y168H occurred independently in different populations.
Assuntos
Ligação Genética , Mutação de Sentido Incorreto , Fenilalanina Hidroxilase/genética , Fenilcetonúrias/genética , Polimorfismo Genético , Haplótipos , Humanos , Sibéria , EspanhaRESUMO
The critical role for GATA family proteins in maintaining the normal (non-transformed) cell state is corroborated by the recent findings of mutations or methylation in GATA genes both in primary cancers and tumor lines including breast. Previously, microarray profiling studies determined that the highest expression of both GATA3 and ESR1 (estrogen receptor alpha) is seen in tumors associated with the most favorable survival outcomes, whereas the lowest expression of GATA3 is detected in tumor subtypes showing the worst outcomes. At this time, genes and pathways that are regulated by GATA3 in the mammary gland are not well defined. We have previously established a requirement for FOG (Friend Of GATA) cofactors during mouse development. Here we report that in the murine mammary gland Fog2 gene expression is upregulated upon pregnancy and lactation with prominent expression in the epithelial cells of the gland during post-lactational regression. Mammary-specific deletion of Fog2 identified a role for this gene during gland involution; excision of the Fog2 gene leads to the accelerated involution of the gland despite diminished levels of the remodeling enzymes. Importantly, the levels of several genes linked to the control of cancerous transformation in the breast (Esr1, Prg and Foxa1) are significantly reduced upon Fog2 excision. This implicates FOG2 in the maintenance of epithelial cell differentiation in the mammary gland and in performing a protective role in breast cancer.
Assuntos
Proteínas de Ligação a DNA/genética , Receptor alfa de Estrogênio/genética , Expressão Gênica , Glândulas Mamárias Animais/citologia , Fatores de Transcrição/genética , Animais , Apoptose , Feminino , Genótipo , Imuno-Histoquímica , Masculino , Glândulas Mamárias Animais/metabolismo , Camundongos , Reação em Cadeia da Polimerase Via Transcriptase ReversaRESUMO
Mutations were studied in phenylalanine hydroxylase gene of phenylketonuria patients from Kemerovo oblast and Altaiskii krai (15 and 2 families, respectively). The following mutations were identified in exons of this gene: R408W, R261Q, R243Q, Y414C, Y386C, P281L, Y168H, R68S (lead to amino acid substitutions), R243X (leads to stop codon formation), and three splice site mutations (IVS12nt 1g-->a, IVS2nt-13t-->g, IVS7nt 1g-->a).
Assuntos
Mutação , Fenilalanina Hidroxilase/genética , Análise de Sequência de DNA/instrumentação , Automação , Sequência de Bases , Códon de Terminação , Primers do DNA , ÉxonsRESUMO
Quantities of Ureaplasma spp DNA were determined by the methods of competitive and multiplex polymerase chain reactions (PCR). The results obtained by each of the methods were shown to be compatible. It was established that the number of Ureaplasma spp. cells per one epithelial cell in the female cervical canal varied from 0.05 to 10.
Assuntos
DNA Bacteriano/análise , Reação em Cadeia da Polimerase/métodos , Ureaplasma/genética , Primers do DNA , DNA Bacteriano/genética , Feminino , Humanos , Fenilalanina Hidroxilase/genética , RNA Ribossômico 16S/genética , Sensibilidade e Especificidade , Ureaplasma/química , Infecções por Ureaplasma/microbiologiaRESUMO
The spectrum and frequency of mutations of exon 7 of the gene for phenylalanine hydroxylase (PAH) were studied in 34 phenylketonuria (PKU) patients living in Novosibirsk oblast. The five most prevalent mutations constituted 17.64% of defective alleles: R243Q (1.47%), R252W (1.47%), R261Q (5.88%), E280K (1.47%), and P281L (7.35%). A neutral polymorphic locus V245V was found within exon 7.
Assuntos
Éxons , Mutação , Fenilalanina Hidroxilase/genética , Fenilcetonúrias/genética , Humanos , Fenilcetonúrias/enzimologia , Fenilcetonúrias/etnologia , Polimorfismo Genético , SibériaRESUMO
A previously unknown sequence of the human phenylalanine hydroxylase (PAH) gene intron 7 (GeneBank AN AF204239) has been reported. Screening of the group of phenylketonuria patients from Nobosibirsk region for polymorphic sites within intron 7 revealed single nucleotide substitutions at intron positions 332, 451, 574 and 791. Polymorphic site at intron position 791 corresponds to one of the eight restriction sites (MspI) utilized for haplotype construction. Analysis of the MspI allele frequencies in 29 phenylketonuria patients showed that the frequency of the MspI+ allele in this group was 79.4%. Polymorphic sites at nucleotide position +97 from the beginning of intron 10, and at nucleotide position -54 from the end of intron 5, were also described. The polymorphic sites revealed can be used as markers for identification of the PAH alleles in population genetic studies, and also serve for diagnostics of phenylketonuria (PKU). The presence of numerous nucleotide substitutions within the intronic sequences confirms highly polymorphic structure of the PAH gene.
Assuntos
Fenilalanina Hidroxilase/genética , Fenilcetonúrias/genética , Polimorfismo Genético , Alelos , Sequência de Bases , Primers do DNA , Desoxirribonuclease HpaII/metabolismo , Frequência do Gene , Humanos , ÍntronsRESUMO
Phenylketonuria (PKU) is a widespread autosome recessive hereditary disease caused by a deficiency of the liver enzyme phenylalanine hydroxylase, which results in the distortion of phenylalanine metabolism and accumulation of toxic metabolites. The knowledge of molecular bases of PKU is of a high social importance as it enables phenotypic correction of the disease in the case of its early diagnostics. This disease is known to be associated with mutations in the phenylalanine hydroxylase gene, the distribution and mutation spectrum having pronounced ethnic and regional features. We studied the spectrum of mutations in the phenylalanine hydroxylase gene in a group of patients with PKU from the Novosibirsk region to reveal 10 missense point mutations, 1 mutation in the splice donor site, and 1 microdeletion. For these mutations, most widely distributed in the region, we used straightforward detection methods based on the restriction fragment length polymorphism (RFLP), artificial constructed restriction sites (ACRS) PCR, and denaturing gradient gel electrophoresis (DGGE).
Assuntos
Fenilalanina Hidroxilase/genética , Fenilcetonúrias/enzimologia , Humanos , Mutação de Sentido Incorreto , Mutação Puntual , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição , Deleção de SequênciaRESUMO
The previously unknown sequences of the coding and 3'-flanking regions of the rat tyrosine aminotransferase gene were determined. The boundaries of exons and repetitive elements were established using computer analysis.
Assuntos
Tirosina Transaminase/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Éxons , Dados de Sequência Molecular , Ratos , Ratos WistarRESUMO
The sequence of the region from -1990 to -580 bp of the human tyrosine aminotransferase gene was determined. Computer analysis revealed putative binding sites for the glucocorticoid-receptor complex which mediates regulation of expression of the gene.