From species descriptions to diversity patterns: the validation of taxonomic data as a keystone for ant diversity studies reproducibility and accuracy.

Research findings in natural sciences need to be comparable and reproducible to effectively improve our understanding of ecological and behavioural patterns. In this sense, knowledge frontiers in biodiversity studies are directly tied to taxonomic research, especially in species-rich tropical regions. Here we analysed the taxonomic information available in 470 studies on Brazilian ant diversity published in the last 50 years. We aimed to quantify the proportion of studies that provide enough data to validate taxonomic identification, explore the frequency of studies that properly acknowledge their taxonomic background, and investigate the primary resources for ant identification in Brazil. We found that most studies on Brazilian ant diversity (73.6%) explicitly stated the methods used to identify their specimens. However, the proportion of papers that provide complete data for the repository institutions and vouchered specimens is vanishingly small (5.8%). Additionally, only 40.0% of the studies consistently presented taxon authorities and years of description, rarely referencing taxonomic publications correctly. In turn, the number of specialists and institutions consulted for ant identification in Brazil has increased in the last years, along with the number of studies that explicitly provide their taxonomic procedures for ant identification. Our findings highlight a shift between generations regarding the recognition of taxonomy as fundamental science, deepening our understanding of biodiversity.

Different trophic groups of arboreal ants show differential responses to resource supplementation in a neotropical savanna.

Resource-ratio theory predicts that consumers should achieve optimal ratios of complementary nutrients. Accordingly, different trophic groups are expected to vary in their N-limitation depending on the extent to which they feed primarily on carbohydrate (CHO) or protein. Among arboreal ants, N-limitation ranges from high (for trophobiont tenders), intermediate (leaf foragers) and low (predators). We report results from a manipulative field experiment in a Brazilian savanna that tests the differential attractiveness of nitrogen and CHO to arboreal ants, as well as experimentally examines changes in broader ant foraging patterns in response to protein and CHO supplementation. Every tree within 32 20 × 20 m plots were supplemented with either protein, CHO; protein + CHO or a water control (n = 8 in each case) for a 7-day period in each of the wet and dry seasons. As predicted, different trophic groups responded differentially to supplementation treatment according to the extent of their N-limitation. The richness and abundance of the most N-limited group (trophobiont tenders) was highest at protein supplements, whereas less N-limited trophic groups showed highest species richness (leaf foragers) or abundance (predators) at CHO supplements. Protein supplementation markedly increased the general foraging abundance of trophobiont tenders, but decreased the abundance of leaf foragers. We attribute the latter to increased competition from behaviorally dominant trophobiont tenders. Our study provides experimental evidence that nutrient availability is a major factor influencing arboreal ant communities, both directly through the provision of different resources, and indirectly through increased competitive pressure.

Similar alpha and beta diversity changes in tropical ant communities, comparing savannas and rainforests in Brazil and Indonesia.

Local biodiversity can be expected to be similar worldwide if environmental conditions are similar. Here, we hypothesize that tropical ant communities with different types of regional species pools but at similar habitat types in Brazil and Indonesia show similar diversity patterns at multiple spatial scales, when comparing (1) the relative contribution of alpha and beta diversity to gamma diversity; (2) the number of distinct communities (community differentiation); and (3) the drivers of ß-diversity (species replacement or species loss/gain) at each spatial scale. In both countries, rainforests and savannas (biome scale) were represented by three landscapes (landscape scale), each with four transects (site scale) and each transect with 10 pitfall traps (local scale). At the local scale, α-diversity was higher and ß-diversity lower than expected from null models. Hence, we observed a high coexistence of species across biomes. The replacement of species seemed the most important factor for ß-diversity among sites and among landscapes across biomes. Species sorting, landscape-moderated species distribution and neutral drift are potential mechanisms for the high ß-diversity among sites within landscapes. At the biome scale, different evolutionary histories produced great differences in ant community composition, so the replacement of species is, at this scale, the most important driver of beta diversity. According to these key findings, we conclude that distinct regional ant species pools from similar tropical habitat types are similarly constrained across several spatial scales, regardless of the continent considered.