RESUMO
The article discusses the possibilities of automation microscopy complexes manufactured by Cellavision and MEKOS to perform the medical analyses of blood films and other biomaterials. The joint work of the complex and physician in the regimen of automatic load stages, screening, sampling and sorting on types with simple morphology, visual sorting of sub-sample with complex morphology provides significant increase of method sensitivity, load decrease and enhancement of physician work conditions. The information technologies, the virtual slides and laboratory telemedicine included permit to develop the representative samples of rare types and pathologies to promote automation methods and medical research targets.
Assuntos
Células Sanguíneas , Ciência de Laboratório Médico/métodos , Microscopia , Telemedicina , Automação/instrumentação , Automação/métodos , Células Sanguíneas/metabolismo , Células Sanguíneas/patologia , Humanos , Ciência de Laboratório Médico/instrumentação , Microscopia/instrumentação , Microscopia/métodos , Robótica/instrumentação , Robótica/métodos , Telemedicina/instrumentação , Telemedicina/métodosRESUMO
Acute massive hemorrhage in rats is accompanied by consistent changes in the morphofunctional characteristics of erythrocytes: diameter, area, polarization, form factor, and integral and specific optical density. Thus, young initially intact rats showed significant increases in the mean diameter, area, and integral optical density at particular time points after hemorrhage, along with increases in erythrocyte polarization and form factor. Vagotomized rats of the same age group responded to hemorrhage only with an increase in the form factor. Initially intact aged rats in these conditions showed increases in the integral optical density and form factor. Aged vagotomized rats showed increases in erythrocyte mean diameter and area. The nature and dynamics of responses to hemorrhage in initially intact and vagotomized (14 days post-operative) animals were significantly different and had age-dependent features. Thus, the most marked changes in young initially intact animals were seen 3-10 h after hemorrhage, while maximum changes were seen at 3 and 96 h in vagotomized animals of the same age. In aged initially intact rats, the greatest changes in study parameters were seen at 3 and 96 h, while the greatest changes were seen in vagotomized rats at 3 and 24 h. The physiological mechanisms of these pathological states are discussed.
Assuntos
Eritrócitos/metabolismo , Eritrócitos/patologia , Hemorragia/metabolismo , Hemorragia/patologia , Vagotomia , Envelhecimento/metabolismo , Envelhecimento/patologia , Animais , Masculino , Ratos , Fatores de TempoRESUMO
The authors consider recommendations on blood smear microscopic tests, possibilities of implementing these recommendations when manual microscopy and automatic microscopy complexes (AMC) are used. Whether one may switch over from the recommendations to the quality standard implemented in practice when AMCs are applied is discussed. Conditions for effectively using AMC models with different functions, performance, and cost are considered.
Assuntos
Automação Laboratorial/métodos , Automação Laboratorial/normas , Células Sanguíneas/patologia , Microscopia/métodos , Microscopia/normas , Robótica/métodos , Robótica/normas , Humanos , Microscopia/tendências , Guias de Prática Clínica como Assunto , Robótica/tendênciasRESUMO
Sharp massive hemorrhage (30-35 % of total amount of blood) in rats are accompanied by natural changes of morpho-functional characteristics of erythrocytes: diameter, the area, polarization, the factor of the form, integrated and specific optical density. Thus, in certain terms, after hemorrhage at young initially intact animals average diameter, the area, integrated optical density actually increase as well as the value of polarization and the factor of the form of erythrocytes growth. In vagotomized rats of the same age group, in reply to hemorrhage, the increase in value of the factor of the form only is obvious. In initially intact old animals in these conditions, an increase of integrated and specific optical density and of the factor of the form also takes place. In old vagotomized rats, the average diameter and the erythrocyte area accrue. Thus, the character and dynamics of response of erythrone in initially intact and vagotomized (through 14 days after operation) animals essentially differ and have certain features dependent on age. Thus, in young initially intact rats, the most expressed changes are marked in 3-10 hours whereas in vagotomized animals of the same age group - in 3 and 96 hours after hemorrhage. In old initially intact rats, the greatest deviations of the investigated parameters are observed in 3 and 96 hours, in the vagotomized animals - in 3 and 24 hours.
Assuntos
Eritrócitos/metabolismo , Eritrócitos/patologia , Hemorragia/metabolismo , Hemorragia/patologia , Vagotomia , Envelhecimento/metabolismo , Envelhecimento/patologia , Animais , Masculino , Ratos , Fatores de TempoRESUMO
MEKOC microscopy complexes have a group of specialized automatic functions for medical analyses of biomaterials integrated with general virtual microscopy accessories. Such functions provide a way of making specialized reference virtual slides (RVS). The latter contain the results of virtual analysis or expert evidence of the automatic analysis results presented in the virtual slide. The use of RVS yields an open system with a step-by-step control of the quality of automatic operations. RVS as realistic preparation models are also used to train staff. The results of step-by-step trials of the MEKOC--2 are presented in the paper.
Assuntos
Células Sanguíneas/citologia , Citodiagnóstico/instrumentação , Microscopia/instrumentação , Autoanálise , Humanos , Contagem de Leucócitos , Controle de Qualidade , Padrões de Referência , Telepatologia/instrumentaçãoRESUMO
Characteristics of modern equipment components for general- or special-purpose modular systems of automated microscopy are considered. Medical, technical, and economic assessment of various configurations of automated microscopy systems is performed. These systems can be used in telemedicine and hematological, cytological, and parasitologic analysis. Schemes for optimization of the configuration of automated microscopy systems are described. The structure of MECOS-Ts2 automated microscopy systems is considered. The system board of MECOS-Ts2 systems makes it possible to use a wide variety of equipment components and software from different manufacturers.
Assuntos
Automação/instrumentação , Microscopia de Vídeo/instrumentação , Microscopia/instrumentação , Óptica e Fotônica/instrumentação , Automação/economia , Humanos , Microscopia/economia , Microscopia de Vídeo/economia , SoftwareRESUMO
Computer-aided and information systems of a MECOS-Ts2 microscopy complex designed to transmit a representative image of a specimen along communication lines are considered. The above systems allow the use of the complex: 1) in on-line and off-line modes to provide tele-medical services (cytology, histology, etc.); moreover, the requirements for channel traffic may be decreased over and over again as compared to the routine on-line videomicroscopy mode; 2) for telemedical tests of specialized local automatic procedures of microscopical analysis (analysis of smears of blood and bone-marrow smears, parasitological analysis, etc.); 3) for collection of a representative electronic archive of the "virtual" specimens of rare nosological entities, which have been obtained in different medical institutions, w hich will provide valid statistical assessments of diagnostic decisions; 4) for remote accompaniment of MECOS-Ts2 complexes by the manufacture (update of versions, training, quality control of sample preparation, collection of commission statistics); 5) for training and certification of remote laboratory staff. The functions considered cannot be implemented in analyzers of other types, in flow analyzers, in particular.
Assuntos
Microscopia/instrumentação , Patologia/instrumentação , Humanos , Processamento de Imagem Assistida por Computador , Software , Telepatologia/instrumentaçãoRESUMO
The paper presents the results of medical tests of a group of computer-aided procedures for microscopic analysis by means of a MECOS-Ts2 complex (ZAO "MECOS", Russia), which have been conducted at the Republican Children's Clinical Hospital, the Research Institute of Emergency Pediatric Surgery and Traumatology, and Moscow City Clinical Hospital No. 23. Computer-aided procedures for calculating the differential count and for analyzing the morphology of red blood cells were tested on blood smears from a total of 443 patients and donors, computer-aided calculation of the count of reticulocytes was tested on 318 smears. The tests were carried out under the US standard NCCLS-H20A. Manual microscopy (443 smears) and flow blood analysis on a Coulter GEN*S (125 smears) were used as reference methods. The quality of collection of samples and laboriousness were additionally assessed. The certified MECOS-Ts2 subsystems were additionally used as reference tools. The tests indicated the advantage of computer-aided MECOS-Tsl2 complex microscopy over manual microscopy.
Assuntos
Testes Hematológicos/instrumentação , Adolescente , Adulto , Idoso , Autoanálise , Contagem de Células Sanguíneas , Criança , Pré-Escolar , Humanos , Microscopia , Pessoa de Meia-Idade , Telemedicina/instrumentaçãoRESUMO
Disadvantages of conventional procedures for testing of medical automated microscopy systems are considered. A testing procedure based on step-by-step testing of the system units is suggested. The procedure makes use of remote data analysis through communication lines. The structure of MECOS-Ts2 automated microscopy system and the procedure for its testing are considered.
Assuntos
Células Sanguíneas/citologia , Células da Medula Óssea/citologia , Citodiagnóstico/instrumentação , Microscopia/instrumentação , HumanosRESUMO
Measurements of the form and size of erythrocytes are needed in the diagnosis of a number of diseases. However, such measurements, if made manually, are a labor-consuming and often inaccurate method, which ensures the determination of a very limited number of parameters. Hardware/software unit MEKOS-C1 enables an automated examination of blood smear, thus speeding up significantly the analysis and ensuring a more complete and accurate information. The possibilities of unit MEKOS-C1 were evaluated for the diagnosis of ovalocytosis. Blood smears of 19 patients from the Russian Pediatric Clinical Hospital, including 8 patients with inherited ovalocytosis, 1 patient with spherocytic ovalocytosis and 10 patients without the disease, were made use of Erythrocytes were isolated in the images of preparations and the contour of each cell was approximated by ellipse. The ratio between ellipse semi-axes served as a measure of erythrocytes' ovality. The mean ratio of semi-axes (RS) and the index of ovalocytosis (IO), i.e. a ratio of the mean maximum diameter to the mean minimal diameter, were calculated for each smear. Manual IO measurements were made in all preparations as a control. Since an additional error can enter the result because of the irregular smear nature and impossibility to standardize completely the technology of smear preparation, the data, obtained from two different smear parts and from parallel preparations, were compared. The reliability, stability and good reproducibility of the automated measurement results were demonstrated. The mean erythrocytes' RS correlated well with IO, obtained manually, and did not virtually differ from RS, measured in the automated manner. The mean RS value of erythrocytes, obtained from patients with inherited ovalocytosis, significantly differed from the control values, which is indicative of a high information density of the discussed parameter. Therefore, RS, when measured automatically, is a reliable and convenient characteristic of erythrocytes' ovality under the conditions of using the ordinary technique of smear preparation.
Assuntos
Técnicas Citológicas/instrumentação , Eliptocitose Hereditária/sangue , Eritrócitos/patologia , Testes Hematológicos/instrumentação , Microscopia/instrumentação , Adolescente , Autoanálise , Tamanho Celular , Criança , Feminino , Humanos , Masculino , RobóticaRESUMO
The most informative for differential diagnosis parameters (surface and nucleus polarization) were determined on the basis of morphometric analysis of 42 cytograms of invasive duct cancer (IDC) and 39 cytograms of invasive lobular breast cancer (ILC) using the computer analyzer MEKOC-C. Combination of these two parameters allowed the authors to elaborate a decisive rule for differential diagnosis of these two cancers (the mistake is reduced to 8.6%). The analysis of 8 informative morphometric nuclear parameters has shown that in ILC the nuclei are more monomorphic, less in square and perimeter, nucleoli are less numerous than in IDC. The study gives objective criteria for preoperative differential cytological diagnosis between IDC and ILC.
Assuntos
Neoplasias da Mama/patologia , Carcinoma Ductal de Mama/patologia , Carcinoma Lobular/patologia , Neoplasias da Mama/diagnóstico , Carcinoma Ductal de Mama/diagnóstico , Carcinoma Lobular/diagnóstico , Diagnóstico Diferencial , Feminino , Humanos , Citometria por Imagem , Processamento de Imagem Assistida por ComputadorRESUMO
Peripheral blood platelets of 25 normal subjects were examined by optic microscopy and flow cytometry. Blood was collected routinely into tubes for Cobas Micros flow scintillator with EDTA anticoagulant. Smears were made as a monolayer and stained by May-Grünwald's method in our modification. Morphologically, 5 classes of platelets were distinguished under optic microscope: young, mature, old, macroplatelets, and irritated forms. The percentage of each class was estimated (i.e., normal platelet formula was determined). Comparison of platelet parameters obtained on a flow cytometer and optic microscopy data for subjects with high percentage of large platelets showed increased mean volume of platelets (MPV) and width of platelet volume (PDW). The curve reflecting platelet distribution by volume in these donors was characterized by strong inclination to the right and a flat plateau.
Assuntos
Plaquetas/citologia , Adulto , Diferenciação Celular , Tamanho Celular , Feminino , Citometria de Fluxo/instrumentação , Citometria de Fluxo/métodos , Humanos , Masculino , Pessoa de Meia-Idade , Contagem de PlaquetasRESUMO
Morphology of peripheral blood erythrocytes was studied in patients with acute leukemia and aplastic anemia by a Russian cytoanalyzer Mekos-C. Twenty-eight patients with acute myeloblastic leukemia, 15 with acute lymphoblastic leukemia, and 11 with aplastic anemia were examined. Erythrocytes (n = 500) were examined in fixed non-stained blood smears. Hemoglobin content and morphometric parameters of each cell were studied and automatic classification of cells was carried out. The data of computer morphodensitometry are compatible with the data of cytochemical studies of the bone marrow erythroid cells (PAS reaction after McManus). The results indicate circulation of erythrocyte subpopulations differing by shape and other signs (hemoglobin content, section area, shape factor) in the blood of patients with acute leukemia and aplastic anemia. The share of pathological erythrocytes in the peripheral blood reflects failure of erythropoiesis.
Assuntos
Células da Medula Óssea/patologia , Eritroblastos/patologia , Eritrócitos/patologia , Hematopoese , Leucemia/sangue , Leucemia/patologia , Doença Aguda , HumanosRESUMO
Automation of routine operations of photomorphometry of blood smears realized in the MEKOS-C cytoanalyzer helped create a new level of using a set of cytological tests, such as assessment of the leukocyte and erythrocyte formulae and of the histogram of hemoglobin distribution in red cells, estimation of leukocyte subtypes, assessment of the characteristics of the nucleolar system and the nucleoli-forming sites. Automated complex analysis of an increase in the measured characteristics of red and white blood cells impossible in common microscopy and flow cytoanalyzers appreciably improves the sensitivity and accuracy of tests and extends the range of their application. Low cost of analysis of a blood smear permits the use of MEKOS-C not only in clinical studies, but even in overall prophylactic screenings of the population.
Assuntos
Células Sanguíneas/citologia , Técnicas Citológicas , Citofotometria , Automação , Nucléolo Celular/ultraestrutura , Citofotometria/instrumentação , Citofotometria/métodos , Eritrócitos/química , Eritrócitos/citologia , Hemoglobinas/análise , Humanos , Leucócitos/classificação , Leucócitos/citologiaRESUMO
Quantitative cytofluorimetric analysis of red cells by the MEKOS-C device provides complete information about peripheral blood erythrocytes, which cannot be obtained by other methods and equipment. An analysis is cheap and sensitive, this recommending it for practical hematology, toxicology, and narcology. The MEKOS-C device permits the preclinical diagnosis of a number of diseases and monitoring their treatment. The device is cheap and available for therapeutic and diagnostic institutions of all levels.