The Effect of the Position Determination Error for Flexible Linear Array Elements on the Tomogram Focusing.

In the article, the study of the quality of tomogram focusing during the inspection of objects with curved surfaces by flexible acoustic array was described. The main goal of the study was theoretically and experimentally define the acceptable deviation limits of the elements' coordinates values. The tomogram reconstruction was performed by the total focusing method. The Strehl ratio was chosen as a criterion for assessing the quality of tomogram focusing. The ultrasonic inspection procedure were simulated and validated experimentally by means of convex and concave curved arrays. In the study, it was proven that the elements coordinates of the flexible acoustic array were determined with an error of no more than 0.18λ and the tomogram image was obtained in sharp focus.

Studies of Angular Resolution for Acoustic Arc Arrays.

Currently, phased arrays are increasingly used in ultrasonic nondestructive testing. One of the most important parameters of ultrasonic nondestructive testing with the application of phased arrays is the angular resolution. This paper presents the results of studies of the angular resolution of concave and convex acoustic arrays in ultrasonic testing with the application of the total focusing method. Computer modeling of concave and convex acoustic arrays consisting of 16, 32 and 64 elements with distances between elements of 0.5 and 1 mm and arc radii of 30 and 60 mm have been performed. The results obtained by computer modeling were confirmed via in situ experiments.

The BTB and CNC homology 1 (BACH1) target genes are involved in the oxidative stress response and in control of the cell cycle.

The regulation of gene expression in response to environmental signals and metabolic imbalances is a key step in maintaining cellular homeostasis. BTB and CNC homology 1 (BACH1) is a heme-binding transcription factor repressing the transcription from a subset of MAF recognition elements at low intracellular heme levels. Upon heme binding, BACH1 is released from the MAF recognition elements, resulting in increased expression of antioxidant response genes. To systematically address the gene regulatory networks involving BACH1, we combined chromatin immunoprecipitation sequencing analysis of BACH1 target genes in HEK 293 cells with knockdown of BACH1 using three independent types of small interfering RNAs followed by transcriptome profiling using microarrays. The 59 BACH1 target genes identified by chromatin immunoprecipitation sequencing were found highly enriched in genes showing expression changes after BACH1 knockdown, demonstrating the impact of BACH1 repression on transcription. In addition to known and new BACH1 targets involved in heme degradation (HMOX1, FTL, FTH1, ME1, and SLC48A1) and redox regulation (GCLC, GCLM, and SLC7A11), we also discovered BACH1 target genes affecting cell cycle and apoptosis pathways (ITPR2, CALM1, SQSTM1, TFE3, EWSR1, CDK6, BCL2L11, and MAFG) as well as subcellular transport processes (CLSTN1, PSAP, MAPT, and vault RNA). The newly identified impact of BACH1 on genes involved in neurodegenerative processes and proliferation provides an interesting basis for future dissection of BACH1-mediated gene repression in neurodegeneration and virus-induced cancerogenesis.

Transcriptome analysis by strand-specific sequencing of complementary DNA.

High-throughput complementary DNA sequencing (RNA-Seq) is a powerful tool for whole-transcriptome analysis, supplying information about a transcript's expression level and structure. However, it is difficult to determine the polarity of transcripts, and therefore identify which strand is transcribed. Here, we present a simple cDNA sequencing protocol that preserves information about a transcript's direction. Using Saccharomyces cerevisiae and mouse brain transcriptomes as models, we demonstrate that knowing the transcript's orientation allows more accurate determination of the structure and expression of genes. It also helps to identify new genes and enables studying promoter-associated and antisense transcription. The transcriptional landscapes we obtained are available online.