Bio-fabrication of thermozyme-based nano-biosensors: their components and present scenario.

An amalgamation of microbiology, biocatalysis, recombinant molecular biology, and nanotechnology is crucial for groundbreaking innovation in developing nano-biomedicines and sensoristics. Enzyme-based nano-biosensor finds prospective applications in various sectors (environmental, pharmaceutical, food, biorefineries). These applications demand reliable catalytic efficiency and functionality of the enzyme under an extreme operational environment for a prolonged period. Over the last few years, bio-fabrication of nano-biosensors in conjunction with thermozymes from thermophilic microbes is being sought after as a viable design. Thermozymes are known for their robustness, are chemically resistant toward organic solvents, possess higher durability for constant use, catalytic ability, and stability at elevated temperatures. Additionally, several other attributes of thermozymes like substrate specificity, selectivity, and sensitivity make them desirable in developing a customized biosensor. In this review, crucial designing aspects of enzyme-based nano-biosensors like enzyme immobilization on an electrode surface, new materials derived from microbial sources (biopolymers based nanocomposites), improvisation measures for sensitivity, and selectivity have been addressed. It also covers microbial biosynthesis of nanomaterials used to develop sensoristic devices and its numerous applications such as wastewater treatment, biorefineries, and diagnostics. The knowledge will pave the way toward creating consistent eco-friendly, economically viable nanostructured-based technologies with broad applicability and exploitation for industrial use in the near future.

Cellulolytic, amylolytic and xylanolytic potential of thermophilic isolates of Surajkund hot spring.

A total of 41 isolates were obtained from various samples (soil, mud, and water) of Surajkund hot spring, Jharkhand, at three different isolation temperatures of 50°C, 60°C, and 70°C. However, our interest was in the thermophilic strains that were isolated at 60°C and 70°C. Four isolates at 70°C (BITSNS038, BITSNS039, BITSNS040, BITSNS041) are the producers of thermozymes, namely amylase, xylanase, and cellulase, respectively. The highlights of the present study also showed that three out of four isolates demonstrated all three enzymatic activities, i.e. amylolytic, xylanolytic and cellulolytic on agar plate assay conditions at 70°C. One of the isolates, BITSNS038, was further chosen for phenotypic characterization as well as 16S rRNA gene sequencing and was affiliated to Geobacillus icigianus. The presence of Geobacillus icigianus was reported first time from hot spring, Surajkund, which showed amylolytic index of 1.58, xylanolytic index of 1.5 and cellulolytic index of 2.3 based on plate assay, and amylase activity of 0.81 U/mL, xylanase activity of 0.72 U/mL and very less cellulase activity of 0.15 U/mL after 24 h of growth in submerged conditions. One isolate at 60°C BITSNS024 was found to exhibit maximum amylase activity with an enzymatic index value of 3.5 and was identified as Anoxybacillus gonensis.

Analysis of gallstone composition and structure in Jharkhand region.

AIM: The aim of this study was to analyze gallstones structurally and chemically as this may help to direct the measures for its treatment. METHODOLOGY: On the basis of morphology, 459 gallstones were categorized into pigmented, cholesterol, and mixed gallstones and analyzed for their chemical structure and composition. Elementary analysis was done with the help of inductively coupled plasma, optical emission spectrophotometry. Fourier transform infrared spectroscopy was used for compound analysis. The effects of thermal stability were analyzed by thermal gravimetric analysis (TGA). Statistical analysis was done to correlate gallstone composition with their shape and number in gallbladder. RESULTS: Pigmented gallstones were predominant and generally occurred in multiple forms, cholesterol as solitaire and black pigmented as slug (χ (2) = 3.56; p < 0.001). Scanning electron microscopy showed the crystals of cholesterol in cholesterol gallstones while pigmented gallstones were more compact in structure. Both types of structure were seen in mixed stones while black pigmented stones were amorphous in nature. This difference in structures might be due to difference in chemical compositions. Cholesterol and mixed stones contained basically cholesterol, brown pigmented constituted bilirubin as a major component, and black stones differed from brown pigmented stones by the presence of Ca palmitate. Bilirubin and palmitate were thermally more stable than cholesterol; hence pigmented gallstones were thermally more stable than cholesterol gallstones, as seen in TGA study. CONCLUSION: Pigmented gallstones were most common in this study of gallstones from the Jharkhand region.