[Glycodelin as a potential marker of female genital system tumors (a review of literature)].

The authors present the currently available data on the expression of glycodelin, a unique secretory protein of the human reproductive system, which has gender-dependent glycosylation, immunosuppressive and morphogenetic activities, in gynecological cancers. They also discuss the theoretical and practical aspects of studying glycodelin as an effector and a marker molecule of oncogenesis.

[Non-developing pregnancy: histological and immunohistochemical markers of endocrine disorders in endometrial scrapes].

Histological and immunohistological (monoclonal antibodies against desmin, vimentin, glycodelin, IGFBP-1, estrogen receptors and progesterone receptors) studies were carried out to examine uterine scrapes from 47 healthy women - medical abortions from 6 to 10 weeks of conception and from 44 patients after non-developing pregnancy in the first trimester (recurrent miscarriage), which had been induced by verified endocrine disorders. Among them, three isolated pathogenetic types were identified: 1) uterine gland retardation as preserved islets of cylindrical glands with a narrow lumen or, on the contrary, areas with enlarged glands and atrophic epitheliocytes; immunohistochemically, they exhibited a drastic reduction in the expression of glycodelin, the major local immunosuppressor; 2) incomplete decidualization when it in the presence of progesterone deficiency was delayed at the state of predecidual and decidual cells retaining the smooth muscle cell marker - desmin; 3) verified progesterone receptor insufficiency. Each of the given clinical and morphological types of endocrine causes of non-developing pregnancy requires that an individual approach be applied to examining and treating these patients.

[Endometrial decidualization as a factor that regulates cytothrophoblastic invasion during the first trimester of pregnancy].

Endometrial stromal cell decidualization is a multistage process that starts with the late secretory phase of a menstrual cycle and then continues in the first months of pregnancy. Under the influence of the hormone progesterone, precursor cells undergo a unique structural and functional transformation from the adventitional cells of spiral arteries containing a marker of smooth muscles (desmin) through pre-decidual cells to the decidual intermediate cells expressing a marker of the extraembryonic mesoderm (vimentin), and IGFBR-1 and then to the typical epithelioid decidual cells.

[Fertility alpha-2-microglobulin expression in the human bulbourethral glands]. / Ekspressiia al'fa 2-mikroglobulina fertil'nosti v bul'bouretral'nykh zhelezakh cheloveka.

Fertility alpha-2-microglobulin (FAMG) was demonstrated in the human bulbourethral glands by the method of indirect immunoperoxidase staining using monoclonal antibodies against FAMG. The product of the immunohistochemical reaction was primary found in the duct cells. Weak immunopositive reaction was observed in single epitheliocytes of the secretory units. FAMG-positive granularity was typically found in the supranuclear cytoplasmic zone, that corresponds to the localization of secretory granules both in the glandulocytes and in the duct cells. These results suggest, that the bulbourethral glands are probably the additional producers of human seminal plasma FAMG.

[Level of placental alpha1-microglobulin in maternal serum as a marker of intrauterine fetal pathology]. / Uroven' platsentarnogo alpha1-mikroglobulina v materinskoi syvorotke kak pokazatel' vnutriutrobnoi patologii ploda.

Serum levels of placental alpha 1-microglobulin (PAMG) were measured during the third trimester in 21 women with normal pregnancy, 24 with extragenital diseases, and 57 with gestosis. Abnormally high levels of PAMG were detected in 23 women, 4 of these with clinically uneventful pregnancy. All pregnancies in the group with high concentrations of PAMG had unfavorable outcomes with the following diagnoses at birth: intrauterine hypoxia (14 cases), low-weight babies (5 cases), small-for-date fetuses (2 cases), preterm delivery (1 case), and asphyxia (1 case). Therefore, a high level of PAMG is an indicator of intrauterine disease even in cases when there are no clinical symptoms of pregnancy abnormalities.

[The content of the PAMG-1 protein that binds insulin-like growth factor I (somatomedin C) in the blood serum of diabetic patients]. / Soderzhanie belka PAMG-1, sviazyvaiushchego insulinopodobnyi faktor rosta 1 (somatomedin C), v syvorotke krovi bol'nykh sakharnym diabetom.

The study was undertaken to measure PAMG-1 (PP 12, IGEBP-1) that is insulin-like growth factor binding protein in blood sera of diabetic patients. We could show a stable significant (10-fold or more in 54% of patients) increase of PAMG-1 concentrations in patients suffering with insulin-dependent diabetes and a moderate (1.5-2 times) increase of PAMG-1 levels in 80% of patients suffering with non-insulin-dependent diabetes. Significant individual differences of DAMG-1 serum concentrations were also demonstrated (10-230 mg/ml in insulin-dependent and 0-360 ng/ml in non-insulin-dependent diabetes).

[Histochemical and clinical-diagnostic study of placental alpha 1-microglobulin using monoclonal antibodies]. / Gistokhimicheskoe i kliniko-diagnosticheskoe izuchenie platsentarnogo alpha 1-mikroglobulina s pomoshch'iu monoklonal'nykh antitel.

Five stable hybridomas against placental protein PAMG-1 were obtained. Monoclonal antibodies were not cross reactive with other placental proteins and human serum albumin. The content of PAMG-1 during a physiological pregnancy increased to 40 ng/ml. 23 women with repeated abortions had an increased content of PAMG-1.

[Isolation and analysis of monoclonal antibodies against various gangliosides]. / Poluchenie i issledovanie monoklonal'nykh antitel protiv nekotorykh gangliozidov.

Female BALB/c mice were immunized with human melanoma (Mewo) cells containing ganglioside GD3 as a surface antigen. Immune splenocytes were fused with syngeneic P3-X63.Ag 8 myeloma cells. Antibodies produced by hybrid clones were analyzed by solid phase immunoassay. B, C, D and Q clones producing antibodies against Raja clavata brain gangliosides were obtained. Monoclonal B and C antibodies bound monosialogangliosides. Monoclonal D antibody bound a number of gangliosides but reacted predominantly with GD1a. Monoclonal Q antibody reacted selectively with GQ1c. It is assumed that ganglioside GQ1c is expressed on the melanoma cell surface and may be found only in the early stage of ontogenesis of high vertebrates.

[Stathmokinetic reaction reversibility in malignantly transformed cells]. / Obratimost' statmokineticheskoi reaktsii v zlokachestvenno transformirovannykh kletkakh.

The statmokinetic reaction of malignant-transformed cells of a hamster tumor (CHT-1) was induced by colcemid. The reversibility of the reaction is primarily related to the repolymerization of spindle microtubules from the tubuline pool of the precursors. The character of the statmokinetic reaction reversibility in the test cells evidences the activity of polymerization processes in them. The course of these processes in the presence of copper ions did not differ from that in nonmalignant-transformed cells.

[Pathology of mitosis after cellular recovery from a metaphase block]. / Patologiia mitoza posle vykhoda kletok iz metafaznogo bloka.

The experiments in Chinese hamster fibroblast-like cells have shown that the c-mitosis inducing agents (colchicine, colcemid and low temperature) produce evident stathmokinetic and radiomimetic effects. By the reversibility moment of the former the latter becomes apparent, manifesting in bridge accumulation. This form of pathology is likely to be caused by the disturbance of the cellular nucleoprotein metabolism in c-mitosis.

[Electron microscopic study of interphase in a culture of fibroblast-like cells]. / Elektronnomikroskopicheskoe issledovanie interfazy v kul'ture fibroblastopodobnykh kletok.

The analysis of the ultrastructural changes of fibroblast-like cells of Chinese hamster at different periods of the cell cycle demonstrates that the most reliable morphological criteria for identification of different periods of the interphase are changes of the nuclear chromatin and nucleolus and the dynamics of development of the daughter centrioles. No prominent changes of the morphology of the protein-synthesizing apparatus during interphase were noted.