1.
J Comp Pathol
; 119(4): 467-72, 1998 Nov.
Artigo
em Inglês
| MEDLINE
| ID: mdl-9839207
RESUMO
Immunohistochemical evaluation of equine tissue necessitates the use of antibodies reactive with cells from a heterogeneous population. Lymphoid tissues from 12 horses were fixed in Bouin's fluid, ethanol or formalin and examined for immunohistochemical reactivity with anti-equine and anti-human monoclonal antibodies (MAbs) specific for MHC Class II antigens, T and B lymphocytes, and macrophages. Only a few of the anti-equine MAbs tested were reactive with fixed, paraffin wax-embedded tissue. Anti-human MAbs expanded the desired range of reactivity and increased the consistency in different animals. The anti-equine MAbs conferred species specificity and anti-human MAbs provided an increased spectrum of reactivity.