RESUMO
We developed a pyrosequencing protocol for monitoring of stability of attenuating mutations in the genome of vaccine reassortants based on master donor virus of Russian live attenuated influenza vaccine B/USSR/60/69. The developed protocol allows rapid and accurate assessment of mutations and can be used for analysis of genetic stability of reassortants during vaccine strain development and manufacturing, as well as genetic stability of vaccine isolates of influenza B virus during pre-clinical and clinical trials.
Assuntos
Análise Mutacional de DNA/métodos , Instabilidade Genômica , Vacinas contra Influenza/genética , Análise de Sequência de DNA/métodos , Vacinas Atenuadas/genética , Animais , Embrião de Galinha , DNA Viral/análise , Humanos , Vacinas contra Influenza/análise , Influenza Humana/prevenção & controle , Tipagem Molecular/métodos , Vacinas Atenuadas/análise , Virologia/métodosRESUMO
The testing of specificity and diagnostic effectiveness was applied to new national chromogenic growth mediums. The algorithm was developed to isolate and express identify the clinically significant and sanitary demonstrative opportunistic enterobacteria using chromogenic growth mediums.
Assuntos
Algoritmos , Compostos Cromogênicos/química , Meios de Cultura/química , Enterobacteriaceae/isolamento & purificação , Infecções Oportunistas/microbiologia , Enterobacteriaceae/crescimento & desenvolvimento , HumanosRESUMO
Studies to design a dry chromogenic nutrient medium for the diferentiation of Klebsiella were under way, by detecting the intracellular Klebsiella genus-specific enzyme of human potential pathogenicity--5-aminosalicylate decarboxylase. The composition of the proposed medium that ensured its high sensitivity and improved its differentiating properties as compared with the known traditional media was worked through. Klebsiella are isolated and identified on the proposed medium in one step, which substantially reduces diagnosis time and material costs and takes some burden from microbiologists.
Assuntos
Klebsiella/crescimento & desenvolvimento , Técnicas Bacteriológicas , Carboxiliases/análise , Meios de Cultura , Humanos , Klebsiella/classificação , Klebsiella/enzimologia , Mesalamina/metabolismoRESUMO
The effectiveness of the bacteriological study of the blood depends in many aspects on the quality of nutrient media. The currently approved nutrient media for the isolation and yield of hemocultures have low isolation rates and require prolonged time for the isolation of causative agents. A two-phase nutrient medium for the yield and isolation of hemoculture has been developed. This medium has been found to have advantages over the control "double medium" in sensitivity, yield effect, growth rate and isolation rate with respect to the causative agents of bacteriemia and sepsis. The medium is standard and ready for use. The above-mentioned advantages of the medium increase the quality of the bacteriological diagnosis of bacteriemia and sepsis.
Assuntos
Bacteriemia/diagnóstico , Bactérias/isolamento & purificação , Técnicas Bacteriológicas/métodos , Bacteriemia/microbiologia , Técnicas Bacteriológicas/normas , Meios de Cultura/normas , Kit de Reagentes para DiagnósticoRESUMO
A dried selective culture medium, electrolyte-deficient sorbitol agar (EDS agar), for the isolation and preliminary identification of E. coli O157:H7 from clinical material has been developed. The medium is not inferior in its quality to analogous foreign media and requires no scarce ingredients for its manufacture.