Relationship of American Indian blood quantum with osteoporosis risk: a cross-sectional study of American Indian women in Oklahoma.

Information regarding the prevalence and risk of osteoporosis among American Indian (AI) women is limited. This study showed that with increasing AI blood quantum, the prevalence of osteoporosis at the hip based on BMD T-scores decreased and this appeared to be independent of other risk factors. INTRODUCTION: This study was designed to investigate the effects of AI blood quantum (BQ) on osteoporosis prevalence and risk in a cohort of AI women in Oklahoma. METHODS: Women (n = 301), aged 50 years and older, were recruited to participate in the Oklahoma American Indian Women's Osteoporosis Study. Baseline bone density, fracture history, bone biochemical markers, and potential risk factors were assessed. Participants were stratified by AI BQ into BQ1 ≤ 25%, BQ2 = 25-49%, BQ3 = 50-74%, and BQ4 = 75-100%. The effects of BQ on the prevalence and risk of osteoporosis were evaluated. RESULTS: Based on T-scores, one in approximately eight women in the study was osteoporotic at one or more sites. The prevalence of osteoporosis decreased (p < 0.05) with increasing BQ, especially at the hip, trochanteric, and intertrochanter regions. No differences in bone-specific alkaline phosphatase and C-telopeptide were observed across BQ that could account for the differences in bone density. 25-OH vitamin D decreased with increasing BQ, but mean for each BQ1-4 was > 40 ng/mL. Fracture history did not differ across BQ, and though 52% of the population consumed less than the RDA for calcium, no effect of BQ was observed. CONCLUSIONS: In this cohort of women who identified as AI, greater Indian BQ was associated with a decrease in the prevalence of osteoporosis.

Correlates of dietary resilience among socioeconomically disadvantaged adolescents.

BACKGROUND/OBJECTIVES: Despite increased risk for unhealthy diets, some socioeconomically disadvantaged adolescents manage to consume a healthy diet, thereby showing 'dietary resilience'. This investigation aimed to describe the vegetable and fruit intakes of socioeconomically disadvantaged adolescents, and explore the intrapersonal, social and environmental factors associated with more favourable dietary intakes among socioeconomically disadvantaged adolescents. SUBJECTS/METHODS: The present investigation draws on data from 1014 socioeconomically disadvantaged adolescents (55% girls), a sub-sample of 3264 adolescents aged 12-15 years recruited from 37 secondary schools in Victoria, Australia. The adolescents completed an online survey in 2004-2005 comprising an FFQ and questions pertaining to intrapersonal, social and environmental factors informed by the Social Cognitive Theory (SCT). Frequent vegetable and fruit intake was defined as 2 times per day and 1 time per day, respectively. RESULTS: Approximately one-third of socioeconomically disadvantaged adolescents frequently consumed vegetables and fruit (boys, 29% and 27% respectively; girls, 29% and 36% respectively). Greater perceived importance of health, and frequently being served vegetables with dinner, were associated with frequent intakes. Friends' support for healthy eating was associated with boys' frequent vegetable intake. Less stringent adherence to family meal-time rules was associated with frequent intakes; however, the opposite was observed when girls were expected to eat all foods served. CONCLUSIONS: Nutrition promotion messages targeting socioeconomically disadvantaged adolescents could focus on fostering appreciation for health and providing families with strategies to increase meal-time vegetable availability. Friends could be encouraged to support healthy eating among boys. Family meal-time rules warrant further investigation.

An amperometric opiate assay.

Current techniques for the detection and measurement of diacetylmorphine (heroin), morphine and their principal metabolite morphine-3-glucuronide (M3G) are based mainly on chromatography or immunoassay. No enzymatic method for the detection of these compounds has yet been reported. Two novel microbial enzymes have been isolated and characterized in this laboratory: an acetylmorphine carboxyesterase (heroin esterase) and a morphine dehydrogenase (MDH). These highly specific enzymes have been incorporated in an amperometric assay for heroin and morphine using phenazine methosulphate as a mediator. The assay gives a rapid and sensitive response to heroin and morphine, with a detection limit for morphine of 6.8 micrograms ml-1 (23.7 microM).

Microbial degradation of the morphine alkaloids. Purification and characterization of morphine dehydrogenase from Pseudomonas putida M10.

The NADP(+)-dependent morphine dehydrogenase that catalyses the oxidation of morphine to morphinone was detected in glucose-grown cells of Pseudomonas putida M10. A rapid and reliable purification procedure involving two consecutive affinity chromatography steps on immobilized dyes was developed for purifying the enzyme 1216-fold to electrophoretic homogeneity from P. putida M10. Morphine dehydrogenase was found to be a monomer of Mr 32,000 and highly specific with regard to substrates, oxidizing only the C-6 hydroxy group of morphine and codeine. The pH optimum of morphine dehydrogenase was 9.5, and at pH 6.5 in the presence of NADPH the enzyme catalyses the reduction of codeinone to codeine. The Km values for morphine and codeine were 0.46 mM and 0.044 mM respectively. The enzyme was inhibited by thiol-blocking reagents and the metal-complexing reagents 1,10-phenanthroline and 2,2'-dipyridyl, suggesting that a metal centre may be necessary for activity of the enzyme.

Biosensors.

This review introduces biosensors as analytical devices that respond selectively to analytes in appropriate samples and convert their concentrations into electrical signals via a combination of a biological recognition system and a suitable transducer. The last decade has seen dramatic advances in the design of sensor configurations, the marriage of biological systems with modern monolithic silicon and optical technologies, the development of effective electron-exchange systems and the introduction of direct immunosensors.

Microbial degradation of the morphine alkaloids: identification of morphine as an intermediate in the metabolism of morphine by Pseudomonas putida M10.

A strain of Pseudomonas putida was isolated by selective enrichment with morphine that was capable of utilising morphine as a primary source of carbon and energy for growth. Experiments with whole cells showed that both morphine and codeine, but not thebaine, could be utilised. A novel NADP-dependent dehydrogenase, morphine dehydrogenase, was purified from crude cell extracts and was shown to be capable of oxidising morphine and codeine to morphinone and codeinone, respectively. This NADP-dependent morphine dehydrogenase was not observed in any other species of pseudomonads examined and was quite distinct from the beta-hydroxysteroid dehydrogenase found in Pseudomonas testosteroni, which had previously been shown to have activity against morphine.

Joseph LeConte on evolution, education, and the structure of knowledge.

During the last quarter of the nineteenth century the theory of evolution profoundly influenced American thought. In the form of Social Darwinism it significantly shaped views on race, civilization, and political theory. Sociology and psychology were developing concurrently as disciplines. All of these were destined to have an effect upon education, for many intellectuals believed that science in general and evolutionary theory in particular were directly applicable to all areas of thought. Among the advocates of such a role for science and evolutionary theory was Joseph LeConte, whose views on the subject are outlined and compared with his contemporaries.