RESUMO
We conducted a randomised controlled study to evaluate whether watching video compact discs intra-operatively using a liquid crystal display (LCD) unit decreased anxiety. Forty-four patients undergoing elective surgery under regional anaesthesia were assigned to either the LCD or control group. Anxiety was measured using the Chinese version of the State-Trait Anxiety Inventory (STAI) and visual analogue score (VAS). The mean (SD) anxiety trait scores were 46.15 (6.28) and 46.40 (7.32) in the control and LCD groups, respectively. The state anxiety of the LCD group [35.50 (7.96)] measured immediately postoperatively was significantly lower than the control group [41.50 (9.02); p = 0.03]. The median (range) reduction in VAS anxiety score was not significantly greater in the LCD group [20 (20 to 80) mm] compared with the control group [12.5 (70 to 60) mm]. Watching video intra-operatively reduces patient anxiety as measured by the STAI.
Assuntos
Anestesia por Condução , Ansiedade/prevenção & controle , Cuidados Intraoperatórios/métodos , Complicações Intraoperatórias/prevenção & controle , Gravação de Videodisco , Adolescente , Adulto , Idoso , Ansiedade/etiologia , Procedimentos Cirúrgicos Eletivos/psicologia , Feminino , Humanos , Complicações Intraoperatórias/psicologia , Masculino , Pessoa de Meia-Idade , Satisfação do Paciente , Escalas de Graduação Psiquiátrica , Fatores de RiscoRESUMO
We have isolated cDNA clones encoding the rodent 14-3-3 zeta and epsilon isoforms by screening bacteriophage expression libraries with a probe derived from the carboxy-terminus of the Vav oncoprotein. These isoforms, however, did not recognize the full length Vav protein under physiological conditions. In agreement with previous studies (see D Morrison, Science 266, 56-57, 1994), these 14-3-3 proteins bound very efficiently to Raf. The interaction between 14-3-3 zeta and Raf involves the central region of 14-3-3 zeta which includes a motif related to annexins. 14-3-3 zeta binds to Raf independently of Ras and forms stable ternary complexes with these two molecules. In contrast to published reports, we have observed that the catalytic activity of Raf was not activated in Raf/14-3-3 zeta immunocomplexes. Likewise, purified preparations of 14-3-3 zeta had no effect on the kinase activity of Raf immunoprecipitates. In addition, Ras activated Raf regardless of whether it was bound or not to 14-3-3 zeta. Finally, overexpression of 14-3-3 zeta cDNA clones in NIH3T3 cells did not result in detectable morphologic transformation even when co-transfected with plasmids encoding Raf and/or Ras proteins. These observations argue against a critical regulatory role of the 14-3-3 proteins in the Raf mitogenic pathway.
Assuntos
Proteínas Serina-Treonina Quinases/metabolismo , Proteínas/fisiologia , Proteínas Proto-Oncogênicas/metabolismo , Tirosina 3-Mono-Oxigenase , Proteínas ras/metabolismo , Proteínas 14-3-3 , Células 3T3 , Sequência de Aminoácidos , Animais , Sítios de Ligação , Camundongos , Dados de Sequência Molecular , Proteínas Proto-Oncogênicas c-raf , Coelhos , Ratos , TransfecçãoRESUMO
Ras-GRF, a guanine-nucleotide exchange factor that activates Ras p21, was tested for its ability to couple to either tyrosine kinase or heterotrimeric G protein signal transduction pathways. Ras-GRF failed to bind the SH2 and SH3 containing adaptor protein Grb2, either in vitro or in vivo. Furthermore, Ras-GRF did not form a stable complex with activated EGF receptor. However, as has been shown previously (Cen et al., 1994), the presence of Ras-GRF in NIH3T3 cells enhanced the activation of Ras induced by serum stimulation. A similar effect was not observed with PDGF stimulation. Moreover, serum stimulation lead to the hyperphosphorylation of Ras-GRF. Both the serum induced super-activation of Ras, and the hyperphosphorylation of Ras-GRF were blocked by pretreatment of cells with the Gi,o inhibitor pertussis toxin, but not by pretreatment with the tyrosine kinase inhibitor genistein. These results suggest that Ras-GRF has the capacity to mediate Ras activation initiated by signals using heterotrimeric G proteins.
Assuntos
Proteínas Adaptadoras de Transdução de Sinal , Receptores ErbB/metabolismo , Proteínas de Ligação ao GTP/metabolismo , Proteínas/metabolismo , Células 3T3 , Animais , Proteínas de Ciclo Celular/metabolismo , Proteína Adaptadora GRB2 , Proteínas Ativadoras de GTPase , Fatores de Troca do Nucleotídeo Guanina , Camundongos , Toxina Pertussis , Fosfoproteínas Fosfatases/metabolismo , Fosforilação , Proteínas/efeitos dos fármacos , Transdução de Sinais , Fatores de Virulência de Bordetella/farmacologia , Proteínas Ativadoras de ras GTPase , Fatores ras de Troca de Nucleotídeo Guanina , ras-GRF1RESUMO
We have used the yeast two-hybrid system to isolate proteins that interact with the carboxy-terminal SH3-SH2-SH3 region of Vav. One of the clones encoded heterogeneous nuclear ribonucleoprotein K (hnRNP K), a poly(rC)-specific RNA-binding protein. The interaction between Vav and hnRNP K involves the binding of the most carboxy-terminal SH3 domain of Vav to two proline-rich sequences present in the central region of hnRNP K. Overexpression of Vav in mouse fibroblasts leads to the formation of a stable complex with the endogenous hnRNP K and to the preferential redistribution of this protein to the cytoplasmic fraction. More importantly, Vav and hnRNP K proteins also interact in hematopoietic cells. In addition, Vav associates in vitro with a second 45-kDa poly(rC)-specific RNA-binding protein via its SH3-SH2-SH3 region. These results suggest that Vav plays a role in the regulation of the late steps of RNA biogenesis by modulating the function of poly(rC)-specific ribonucleoproteins.
Assuntos
Proteínas de Ciclo Celular , Poli C/metabolismo , Proteínas Proto-Oncogênicas/metabolismo , Proto-Oncogenes , Proteínas de Ligação a RNA/metabolismo , Ribonucleoproteínas/metabolismo , Sequência de Aminoácidos , Sequência de Bases , Clonagem Molecular , DNA Complementar , Eletroforese em Gel de Poliacrilamida , Biblioteca Gênica , Células HeLa , Ribonucleoproteínas Nucleares Heterogêneas Grupo K , Ribonucleoproteínas Nucleares Heterogêneas , Humanos , Dados de Sequência Molecular , Oligodesoxirribonucleotídeos , Prolina , Ligação Proteica , Proto-Oncogene Mas , Proteínas Proto-Oncogênicas/biossíntese , Proteínas Proto-Oncogênicas/isolamento & purificação , Proteínas Proto-Oncogênicas c-vav , RNA Nuclear Heterogêneo/metabolismo , Proteínas de Ligação a RNA/biossíntese , Proteínas de Ligação a RNA/isolamento & purificação , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/metabolismo , Ribonucleoproteínas/biossíntese , Ribonucleoproteínas/isolamento & purificação , Saccharomyces cerevisiae , Especificidade por SubstratoRESUMO
NMR spectroscopy has been used to characterize the protein-protein interactions between the mouse Grb2 (mGrb2) N-terminal SH3 domain complexed with a 15-residue peptide (SPLLPKLPP-KTYKRE) corresponding to residues 1264-1278 of the mouse Sos-2 (mSos-2) protein. Intermolecular interactions between the peptide and 13C-15N-labeled SH3 domain were identified in half-reverse-filtered 2D and 3D NOESY experiments. Assignments for the protons involved in interactions between the peptide and the SH3 domain were confirmed in a series of NOESY experiments using a set of peptides in which different leucine positions were fully deuterated. The peptide ligand-binding site of the mGrb2 N-terminal SH3 domain is defined by the side chains of specific aromatic residues (Tyr7, Phe9, Trp36, Tyr52) that form two hydrophobic subsites contacting the side chains of the peptide Leu4 and Leu7 residues. An adjacent negatively charged subsite on the SH3 surface is likely to interact with the side chain of a basic residue at peptide position 10 that we show to be involved in binding. The peptide-binding site of the SH3 is characterized by large perturbations of amide chemical shifts when the peptide is added to the SH3 domain. The mGrb2 N-terminal SH3 domain structure in the complex is well-defined (backbone RMSD of 0.56 +/- 0.21 calculated over the backbone N, C alpha, and C atoms of residues 1-54). The structure of the peptide in the complex is less well-defined but displays a distinct orientation.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Proteínas Adaptadoras de Transdução de Sinal , Oligopeptídeos/química , Fragmentos de Peptídeos/química , Proteínas/química , Sequência de Aminoácidos , Animais , Sítios de Ligação , Gráficos por Computador , Proteína Adaptadora GRB2 , Fatores de Troca do Nucleotídeo Guanina , Espectroscopia de Ressonância Magnética , Camundongos , Dados de Sequência Molecular , Ligação Proteica , Conformação Proteica , Proteínas RecombinantesRESUMO
Vav is a proto-oncogene specifically expressed in cells of hematopoietic origin. Its gene product contains a series of structural motifs, including SH2 and SH3 domains, suggestive of a role in signal transduction. The Vav protein also possesses a Dbl-homology (DH) domain previously found in regulators of the Ras superfamily of small GTP-binding proteins. Recently, Vav has been reported to be the major Ras GDP/GTP exchange factor (GEF) in hematopoietic cells [Gulbins et al., Science 260, 822 (1993); J. Immunol. 152, 2123 (1994)]. The following observations are inconsistent with such a role: (i) Vav proteins do not exhibit Ras GEF activity in standard GDP/GTP exchange assays; (ii) Cells overexpressing Vav do not have increased levels of GTP-bound Ras proteins; (iii) Overexpression of Vav does not overcome the growth inhibitory activity of RasN17, a mutant that blocks Ras signaling by inhibiting Ras GEFs; (iv) Transformation of NIH3T3 cells by Vav oncoproteins is not inhibited by a farnesyl transferase inhibitor that completely blocks transformation by both Ras and its well characterized GEF, RasCDC25 and (v) The morphology of Vav-transformed NIH3T3 cells is dramatically different from that induced by Ras and RasCDC25. Whereas these observations make it unlikely that Vav functions either as a RasGEF or as an upstream regulatory element of Ras, we have observed that Vav can cooperate with normal Ras proteins to transform NIH3T3 cells. These results suggest that Vav and Ras may mediate signal transduction by distinct, but interactive mitogenic pathways.
Assuntos
Alquil e Aril Transferases , Proteínas de Ciclo Celular , Transformação Celular Neoplásica , Genes ras , Guanosina Difosfato/metabolismo , Guanosina Trifosfato/metabolismo , Proteínas/fisiologia , Proteínas Proto-Oncogênicas/fisiologia , Células 3T3 , Animais , Farnesiltranstransferase , Fibroblastos , Fatores de Troca do Nucleotídeo Guanina , Camundongos , Proteínas Proto-Oncogênicas/genética , Proteínas Proto-Oncogênicas c-vav , Proteínas Proto-Oncogênicas p21(ras)/fisiologia , Transdução de Sinais , Transferases/antagonistas & inibidores , Fatores ras de Troca de Nucleotídeo GuaninaRESUMO
This article describes a new technique of intraosseous screw fixation of the cervical spine, as well as a retrospective review of 27 patients who had anterior cervical interbody fusion after diskectomy and fixation with one intraosseous Herbert screw, with a minimum follow-up of 1 year. The study included 19 men and eight women. There were no neurologic complications at final follow-up evaluation. All patients had radiographic evidence of fusion. No screw breakage, back-out, or dislodgement occurred. Optimal intraoperative radiographic evaluation for accurate intraosseous screw placement is recommended. The use of intraosseous screw fixation is a useful addition to the armamentarium of the spine surgeon when fixation of anterior cervical graft after diskectomy is required. One hundred percent rate of union and prevention of complications related to the currently used anterior fixation systems are the major advantages of this method.
Assuntos
Parafusos Ósseos , Transplante Ósseo , Vértebras Cervicais/cirurgia , Discotomia , Ortopedia/métodos , Fusão Vertebral/métodos , Adulto , Idoso , Vértebras Cervicais/diagnóstico por imagem , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Radiografia , Estudos Retrospectivos , Resultado do TratamentoRESUMO
Interleukin-2 (IL-2) is a growth factor involved in the clonal expansion of antigen-activated T lymphocytes. Interaction of IL-2 with its receptor triggers tyrosine phosphorylation of a series of proteins and results in the activation of p21ras. We report here that Shc, an SH2-containing adaptor protein, is tyrosine-phosphorylated following IL-2 stimulation. IL-2-induced tyrosine phosphorylation of Shc was detectable within seconds following IL-2 addition, reaching its highest level by 15 min. Tyrosine phosphorylation of Shc was induced in multiple IL-2-dependent T cell lines and was found to correlate with IL-2-dependent cell proliferation. Tyrosine-phosphorylated Shc was found to be capable of associating with the SH2 domain of Grb2 following IL-2 stimulation. These results indicate that tyrosine phosphorylation of Shc and its association with Grb2 may be important events in IL-2-initiated signal transduction events in T cells.
Assuntos
Proteínas Adaptadoras de Transdução de Sinal , Proteínas Adaptadoras de Transporte Vesicular , Interleucina-2/farmacologia , Proteínas/metabolismo , Linfócitos T/citologia , Tirosina/metabolismo , Animais , Divisão Celular/efeitos dos fármacos , Linhagem Celular , Camundongos , Fosforilação , Proteínas Adaptadoras da Sinalização Shc , Transdução de Sinais , Proteína 1 de Transformação que Contém Domínio 2 de Homologia de Src , Linfócitos T/metabolismoRESUMO
Surgical treatment of type II odontoid fractures (OFs) has usually entailed C1-2 arthrodesis rather than fracture fixation. An alternative treatment of direct screw fixation is used to treat the fractures for preservation of atlantoaxial rotation. Type II OFs that cannot be completely reduced by close means are generally believed to be a contraindication for anterior screw fixation. Seven patients (group I) with displaced type II OFs that could be completely reduced were treated with fracture fixation by one 4.5-mm double-threaded compression screw and five patients (group II) with displaced type II OFs that could only be partially reduced were treated with fracture fixation by one 3.0-mm double-threaded compression screw. All patients had a minimum of 1-year follow-up. No major complications occurred. No loss of reduction occurred in group I patients. Group II patients had an average loss of reduction of 0.8 mm anterior displacement and 5 degrees anterior angulation. The overall rate of fracture union was 100%, and fracture resolution averaged 4.1 months. Ten patients had a normal range of cervical rotation, and there was no difference in preservation of cervical rotation between the two groups. Our results suggest that close reduction and compressive osteosynthesis by one double-threaded compression screw is an optimal method of treatment for displaced type II OFs that can be completely reduced and for some cases that can only be partially reduced. A 100% rate of fracture union and preservation of cervical rotation are the major advantages of this method. However, significant complications have been reported by other investigators. (ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Parafusos Ósseos , Fixação Interna de Fraturas , Processo Odontoide/lesões , Fraturas da Coluna Vertebral/cirurgia , Adulto , Desenho de Equipamento , Feminino , Consolidação da Fratura , Humanos , Masculino , Processo Odontoide/diagnóstico por imagem , Processo Odontoide/cirurgia , Pressão , Radiografia , Fraturas da Coluna Vertebral/diagnóstico por imagemRESUMO
We report the isolation and molecular characterization of the mouse grb2 gene. The product of this gene, the Grb2 protein, is highly related to the Caenorhabditis elegans sem-5 gene product and the human GRB2 protein and displays the same SH3-SH2-SH3 structural motifs. In situ hybridization studies revealed that the mouse grb2 gene is widely expressed throughout embryonic development (E9.5 to P0). However, grb2 transcripts are not uniformly distributed, and in certain tissues (e.g., thymus) they appear to be regulated during development. Recent genetic and biochemical evidence has implicated the Grb2 protein in the signaling pathways that link cell surface tyrosine kinase receptors with Ras. We have investigated the association of the Grb2 protein with epidermal growth factor (EGF) and nerve growth factor (NGF) receptors in PC12 pheochromocytoma cells. EGF treatment of PC12 cells results in the rapid association of Grb2 with the activated EGF receptors, an interaction mediated by the Grb2 SH2 domain. However, Grb2 does not bind to NGF-activated Trk receptors. Mitogenic signaling of NGF in NIH 3T3 cells ectopically expressing Trk receptors also takes place without detectable association between Grb2 and Trk. These results suggest that whereas EGF and NGF can activate the Ras signaling pathway in PC12 cells, only the EGF receptor is likely to do so through a direct interaction with Grb2. Finally, binding studies with glutathione S-transferase fusion proteins indicate that Grb2 binds two distinct subsets of proteins which are individually recognized by its SH2 and SH3 domains. These observations add further support to the concept that Grb2 is a modular adaptor protein.
Assuntos
Proteínas Adaptadoras de Transdução de Sinal , Receptores ErbB/metabolismo , Proteínas/genética , Receptores de Fator de Crescimento Neural/metabolismo , Células 3T3 , Sequência de Aminoácidos , Animais , Sequência de Bases , Caenorhabditis elegans/metabolismo , Transformação Celular Neoplásica , Clonagem Molecular , Proteína Adaptadora GRB2 , Expressão Gênica , Genes , Camundongos , Dados de Sequência Molecular , Oligodesoxirribonucleotídeos/química , Proteínas Oncogênicas/metabolismo , Células PC12 , Fosfoproteínas/metabolismo , Fosfotirosina , Ligação Proteica , RNA Mensageiro/genética , Proteínas Recombinantes de Fusão/imunologia , Alinhamento de Sequência , Tirosina/análogos & derivados , Tirosina/metabolismoRESUMO
We have examined the expression of the vav protooncogene during mouse embryogenesis using RNase protection assays, in situ hybridization, and immunocytochemical analysis. vav gene transcripts were first detected in E11.5 embryos in the blood-forming islands and megakaryocytes of the fetal liver. During diversification of hematopoietic activity in the embryo, vav gene expression became down-regulated in the liver and activated in thymus and spleen. In newborn animals, vav expression was also confined to hematopoietic tissues, with the exception of the ameloblastic cell layer at the latest stages of tooth morphogenesis. In the adult, vav transcripts were found in spleen, thymus, lymph nodes, and bone marrow, but not in liver. In spleen, vav transcripts were concentrated in the white pulp areas, whereas in the red pulp, the vav transcripts appeared to be primarily localized in the megakaryocytes. In thymus, vav expression was found to be more abundant in the cortical areas than in the medulla. In agreement with these observations, purified thymic lymphocytes showed heterogeneous immunoreactivity against the Vav protein, whereas splenic lymphocytes and bone marrow-derived cells displayed rather uniform levels of expression. These observations suggest that the vav protooncogene plays an important role in the signal transduction pathways that regulate the development and maintenance of the hematopoietic system.
Assuntos
Expressão Gênica , Sistema Hematopoético/crescimento & desenvolvimento , Proto-Oncogenes , Animais , Medula Óssea/crescimento & desenvolvimento , Desenvolvimento Embrionário e Fetal/genética , Sistema Hematopoético/embriologia , Sistema Hematopoético/metabolismo , Imuno-Histoquímica , Hibridização In Situ , Fígado/embriologia , Fígado/crescimento & desenvolvimento , Linfonodos/crescimento & desenvolvimento , Camundongos , Camundongos Endogâmicos , RNA Mensageiro/biossíntese , Baço/embriologia , Baço/crescimento & desenvolvimento , Timo/embriologia , Timo/crescimento & desenvolvimento , Saco Vitelino/fisiologiaRESUMO
Recently, a novel type of calcium-dependent protein kinase (CDPK) that requires neither calmodulin nor phospholipids for activation, has been described in plants. We have isolated a cDNA clone for carrot CDPK by probing a library of somatic embryo cDNAs with oligonucleotides corresponding to highly conserved regions of protein kinases. The product of this gene overexpressed in Escherichia coli reacted strongly with monoclonal antibodies to soybean CDPK. The deduced amino acid sequence of carrot CDPK reveals two major functional domains. An N-terminal catalytic domain with greatest homology to calcium/calmodulin-dependent protein kinase type II from rat brain is coupled to a C-terminal calcium-binding domain resembling calmodulin. These features of the primary sequence explain how CDPK binds calcium and suggest a model for CDPK regulation based on similarities to animal calcium/calmodulin-dependent protein kinases.
Assuntos
Calmodulina/genética , Plantas/enzimologia , Proteínas Quinases/genética , Homologia de Sequência do Ácido Nucleico , Sequência de Aminoácidos , Anticorpos Monoclonais/metabolismo , Sequência de Bases , Northern Blotting , Southern Blotting , Cálcio/metabolismo , Calmodulina/metabolismo , Clonagem Molecular , Escherichia coli/metabolismo , Immunoblotting , Dados de Sequência Molecular , Plantas/genéticaRESUMO
Pyruvate formate-lyase (EC 2.3.1.54), a key enzyme in the anaerobic metabolism of Salmonella typhimurium, catalyzes the conversion of pyruvate to acetyl coenzyme A and formate. pfl::Mu dA operon fusions were isolated for the study of transcriptional regulation. pfl was transcribed both aerobically and anaerobically, but the activity increased about sixfold under anaerobic conditions. The addition of pyruvate, formate, and acetate in nutrient broth did not have any effect on the anaerobic expression of pfl. However, the addition of pyruvate to minimal glucose medium increased the anaerobic expression of pfl. The expression of pfl varied in different growth media. Anaerobic expression of pfl was lower when the culture was grown in minimal glucose medium than when it was grown in nutrient broth. When Casamino Acids (Difco Laboratories, Detroit, Mich.) were added to minimal glucose medium, the expression of pfl increased proportionally with the amount of Casamino Acids added. The transcription of pfl was positively controlled by the oxrA gene product and was affected by both the cya and crp mutations. However, mutations in genes affecting the cyclic AMP-cyclic AMP receptor protein complex or oxrA could not completely abolish the anaerobic derepression of pfl. In merodiploid strains, pfl::Mu dA/F' pfl+, the beta-galactosidase activities were decreased. The mutations gyrA, oxrC, and oxrE, which affected anaerobic metabolism, did not affect anaerobic expression of pfl.