Pitfalls in chest CT findings of COVID-19 patients infected during hospitalisation.

OBJECTIVE: This study evaluated the differences in the appearance of COVID-19 pneumonia on chest computed tomography (CT) images of outpatient and cases that developed during hospitalisation. METHOD: Chest CT images of 66 patients (median age, 76 years; range, 29-94 years) who underwent the severe acute respiratory syndrome coronavirus-2 reverse-transcription polymerase chain reaction (RT-PCR) test were included in this retrospective study. The chest CT appearance was categorised as "typical," "indeterminate," "atypical," or "negative" in accordance with the recommendations of the Radiological Society of North America for COVID-19 pneumonia and compared among the following four subgroups: PCR-positive outpatient (n = 14); PCR-positive hospitalised (n = 7); PCR-negative outpatient (n = 9); and PCR-negative hospitalised (n = 36). FINDINGS: The frequency of "typical" findings in the PCR-positive outpatient cases (13/14, 92.9%) was significantly higher than that of those in the PCR-positive hospitalised cases (2/7, 28.6%, P = 0.022). There was no significant difference between the frequency of the "typical" appearance in PCR-positive hospitalised cases and that of those in the PCR-negative hospitalised cases (1/36, 2.8%, P = 0.192). CONCLUSIONS: When COVID-19 patients acquire infections while hospitalised, their chest CT images are less likely to show typical findings than those of outpatient cases. Comprehensive and careful assessments of CT findings and consideration of the possibility of concomitant infections with other pathogens and clinical information, such as underlying diseases, background lung structure, and time course of the infection, are required for the management of such cases.

Evolution of local conductance pathways in a single-molecule junction studied using the three-dimensional dynamic probe method.

Understanding of the dynamics of the bonding states of molecules with electrodes while the molecular conformation is changed is particularly important for elucidating the details of electrochemical devices as well as molecular devices in which the reaction dynamics of the electrodes and molecules plays an important role, such as in fuel cells, catalysis and bioelectrochemical devices. However, it has been difficult to make measurements when the distance between counter electrodes is short, namely, the molecule is raised from a lying form, almost parallel and close to the electrodes, toward a standing form and vice versa. We previously have developed a method called the three-dimensional (3D) dynamic probe method, which enables conductance measurement while the conformation of a single-molecule junction is precisely controlled by scanning tunneling microscopy (STM) techniques. Here, by combining this method with density functional theory (DFT) calculations, it has become possible to simultaneously consider the effects of the dynamics of molecular structures and the bonding states at the electrodes on the local transmission pathways, local-bond contributions to conductance. Here, by performing an analysis on 1,4-benzenediamine (BDA) and 1,4-benzenedithiol (BDT) single molecule junctions, we have observed, for the first time, the effect of a change in the molecular conformations and bonding states on the local transmission pathways for a short Au electrode distance condition.

The effect of nitrogen lone-pair interaction on the conduction in a single-molecule junction with amine-Au bonding.

We have applied our previously developed three-dimensional dynamic probe method to analyze the conductance in a Au-/1,4-benzenediamine (BDA)/Au single molecule junction. This structure is a typically used example to demonstrate the high performance of the break junction (BJ) method for measuring conductance with small variations, however, details of the interaction of the nitrogen (N) lone-pair in the amine group with a Au electrode, which is considered to have a fundamental role in determining the conductance of the single molecule junction with the amine, have not yet been clarified and still remain an important issue to be resolved. In this study, we have succeeded, for the first time, in observing the site-dependent change in conductance of this system while the molecular conformation was accurately controlled, and the results were well reproduced by a simulation taking account of the effect of the N lone-pair in an amine bonding with a Au electrode.

Revealing the Conformational Dynamics in a Single-Molecule Junction by Site- and Angle-Resolved Dynamic Probe Method.

Single-molecule junctions have been extensively studied because of their high potential for future nanoscale device applications as well as their importance in basic studies for molecular science and technology. However, since the bonding sites at an electrode and the molecular tilt angles, for example, cannot be determined experimentally, analyses have been performed assuming the structures of such interactive key factors, with uncertainties and inconsistencies remaining in the proposed mechanisms. We have developed a methodology that enables the probing of conformational dynamics in single-molecule junctions simultaneously with the direct characterization of molecular bonding sites and tilt angles. This technique has revealed the elemental processes in single-molecule junctions, which have not been clarified using conventional methods. The mechanisms of the molecular dynamics in 1,4-benzenedithiol and 4,4'-bipyridine single-molecule junctions, which, for example, produce binary conductance switching of different types, were clearly discriminated and comprehensively explained.