Establishment of an interaction model of human apolipoprotein H with lipid monolayer by capillary SDS gel electrophoresis.

Apolipoprotein H (ApoH) is a plasma glycoprotein isolated from human serum. It plays a key role in the interaction with lipids. For the first time, the concentration of ApoH adsorbed on the lipid monolayer has been determined, and was done so using capillary electrophoresis. Based on this determination, an interaction model of ApoH and lipid monolayer was constructed, and this interaction is one of nonspecific adsorption. A neutral coated capillary (50 cm x 100 microm i.d.) and a negative voltage of 15 kV were used to separate ApoH. The calibration curve of ApoH was built using a detection limit of 50 microg/mL(-1) (near to 1 microM), and the RSD of the relative migration time of ApoH was 1.4% (n = 7).