RESUMO
This study utilized computational simulation and surface molecular imprinting technology to develop a magnetic metal-organic framework molecularly imprinted polymer (Fe3O4@ZIF-8@SMIP) capable of selectively recognizing and detecting multiple fluoroquinolones (FQs). The Fe3O4@ZIF-8@SMIP material was synthesized using the "common" template-ofloxacin, identified by computational simulation, demonstrating notable adsorption capacity (88.61-212.93 mg g-1) and rapid mass-transfer features (equilibration time: 2-3 min) for all tested FQs, consistent with Langmuir adsorption model. Subsequently, this material was employed as a magnetic solid-phase-extraction adsorbent for adsorption and detection of multiple FQs by combining with high performance liquid chromatography. The developed method exhibited good linearity for various FQs within the concentration range of 0.1-500 µg L-1, with low limit of detection (0.0605-0.1529 µg L-1) and limit of quantitation (0.2017-0.5097 µg L-1). Satisfactory recoveries (88.38-103.44%) were obtained when applied to spiked food samples, demonstrating the substantial potential of this Fe3O4@ZIF-8@SMIP material for rapid enrichment and identification for multiple FQs residues.
Assuntos
Fluoroquinolonas , Contaminação de Alimentos , Estruturas Metalorgânicas , Impressão Molecular , Extração em Fase Sólida , Adsorção , Estruturas Metalorgânicas/química , Fluoroquinolonas/análise , Fluoroquinolonas/química , Extração em Fase Sólida/instrumentação , Extração em Fase Sólida/métodos , Contaminação de Alimentos/análise , Cromatografia Líquida de Alta Pressão , Polímeros Molecularmente Impressos/química , Simulação por Computador , Limite de DetecçãoRESUMO
In this work, the perovskite fluorescent nanocrystals (CsPbBr3) were successfully synthesized and wrapped with SiO2 shell, utilized for the assembly of solid-state detection strip capable of conveniently and specifically detection of aflatoxin B1 (AFB1). The SiO2 coating aimed to enhance the stability of CsPbBr3 nanocrystals. The resulting CsPbBr3@SiO2 material exhibited remarkable fluorescence properties, and further self-assembled onto solid-state plate, generating AFB1-specific quenched fluorescence at a specific wavelength of 515 nm. When combined with the capture of AFB1 by magnetic nanoparticles conjugated with aptamers (MNPs-Apt), it was achieved the good separation and specific detection of AFB1 toxin in food matrices. The constructed fluorescent solid-state detection strip based on CsPbBr3@SiO2 exhibited good response to AFB1 toxin within a linear range of 0.1-100 ng mL-1 and an impressive detection limit as low as 0.053 ng mL-1. This presents a new strategy for the rapid screening and convenient detection of highly toxic AFB1.
Assuntos
Aflatoxina B1 , Aptâmeros de Nucleotídeos , Compostos de Cálcio , Contaminação de Alimentos , Nanopartículas , Óxidos , Dióxido de Silício , Titânio , Aflatoxina B1/análise , Aflatoxina B1/química , Contaminação de Alimentos/análise , Dióxido de Silício/química , Compostos de Cálcio/química , Óxidos/química , Nanopartículas/química , Titânio/química , Aptâmeros de Nucleotídeos/química , Limite de Detecção , FluorescênciaRESUMO
The development of sensitive, facile, cost-effective and eco-friendly sensors is essential for monitoring imidacloprid (IDP) residue on a large scale. Compared with popular modification of electrodes with advanced materials, electrochemical activation is promising at this point. In this paper, we found that strongly basic electrolytes (e.g. KOH and K3PO4) and applying cyclic potential during the activating process are beneficial to greatly amplify the electro-reduction response of IDP by nearly 16 times. Combining the characterization of activated electrodes with electrochemical behavior analysis of IDP, it is speculated that specific oxygen-contained functional groups were formed to bond with IDP molecules, leading to fast electron transfer kinetics. Then a sensitive IDP sensor has been developed with a low limit of detection (LOD) of 0.03 µM in the range of 0.1-100 µM. The methodological evaluation including reproducibility, stability and recovery has been also carefully studied, verifying the potential of proposed activated electrodes for application in rice samples.
Assuntos
Oryza , Eletrodos , Neonicotinoides , Nitrocompostos , Reprodutibilidade dos TestesRESUMO
A new 3aminophenylboronic acid-functionalized stationary phase based on silica for hydrophilic interaction liquid chromatography (HILIC) was developed and showed great HILIC characteristics on separation for chitooligosaccharides. The material was synthesized by grafting 3aminophenylboronic acid group to silica, and it was characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), elemental analysis and thermal gravimetric analysis (TGA). Nucleobases and nucleosides were used to evaluate the retention property and to investigate retention mechanism by the models designed for description of partitioning and surface adsorption through adjusting ratio of water in the mobile phase. Parameters affecting chromatography behavior such as ionic strength, buffer pH and column temperature were also investigated. Results have indicated that the retention mechanism was a combination of partitioning and surface adsorption, and the hydrogen bond seemed to be the main force for the retention behavior. Finally, the new 3aminophenylboronic acid-functionalized based on silica stationary phase was applied to separate chito-oligosaccharide samples with optimized mobile phase conditions and showed acceptable chromatograms.