Regulatory role of Mycobacterium tuberculosis MtrA on dormancy/resuscitation revealed by a novel target gene-mining strategy.

Introduction: The unique dormancy of Mycobacterium tuberculosis plays a significant role in the major clinical treatment challenge of tuberculosis, such as its long treatment cycle, antibiotic resistance, immune escape, and high latent infection rate. Methods: To determine the function of MtrA, the only essential response regulator, one strategy was developed to establish its regulatory network according to high-quality genome-wide binding sites. Results and discussion: The complex modulation mechanisms were implied by the strong bias distribution of MtrA binding sites in the noncoding regions, and 32.7% of the binding sites were located inside the target genes. The functions of 288 potential MtrA target genes predicted according to 294 confirmed binding sites were highly diverse, and DNA replication and damage repair, lipid metabolism, cell wall component biosynthesis, cell wall assembly, and cell division were the predominant pathways. Among the 53 pathways shared between dormancy/resuscitation and persistence, which accounted for 81.5% and 93.0% of the total number of pathways, respectively, MtrA regulatory genes were identified not only in 73.6% of their mutual pathways, but also in 75.4% of the pathways related to dormancy/resuscitation and persistence respectively. These results suggested the pivotal roles of MtrA in regulating dormancy/resuscitation and the apparent relationship between dormancy/resuscitation and persistence. Furthermore, the finding that 32.6% of the MtrA regulons were essential in vivo and/or in vitro for M. tuberculosis provided new insight into its indispensability. The findings mentioned above indicated that MtrA is a novel promising therapeutic target for tuberculosis treatment since the crucial function of MtrA may be a point of weakness for M. tuberculosis.

Metal-organic framework derived hollow nitrogen-doped carbon sphere with cobalt phosphide in carbon nanotube for efficient oxygen evolution.

The sluggish kinetics of the electrocatalytic oxygen evolution reaction (OER) pose a significant challenge in the field of overall water splitting. Transition metal phosphides have emerged as promising catalysts for OER by modulating the charge distribution of surrounding atoms. In this study, we employed self-sacrificing templates to fabricate hollow N-doped carbon spheres containing small-sized Co2P embedded within carbon nanotubes through high-temperature calcination and phosphorization, referred to as HNCS-CNT-CoP. The obtained HNCS-CNT-CoP electrocatalyst exhibited excellent OER performance in an alkaline electrolyte due to the optimization of OH* adsorption energy and the large specific surface area created by the hollow structure. It demonstrated a low overpotential of 302 mV at a current density of 10 mA cm-2 and a low Tafel slope of 68.5 mV dec-1, attributed to the electron transport facilitated by the in situ formed carbon nanotubes. Furthermore, theoretical calculations revealed a suitable reaction energy (1.17 eV) in the critical formation of Co2P-*OOH for HNCS-CNT-CoP, significantly lower than the the rate-determining step of HNCS-CNT-Co (10.08 eV). These findings highlight the significance of hollow structures and Co2P-doping in the design of highly active non-noble metal OER electrocatalysts, enabling the reduction of energetic reaction barriers for future applications.

P, N, and C-related functional genes in SBR system promoted antibiotics resistance gene transmission under polystyrene microplastics stress.

Wastewater treatment plants (WWTPs) are important sinks of microplastics (MPs) and antibiotics resistance genes (ARGs). Information regarding connections between functional modules of WWTPs and spread of ARGs under MPs stress is still lacking. In this study, correlations between P-, N-, and C-related functional genes and ARGs in a sequencing batch reactor (SBR) system were evaluated under polystyrene (PS) MPs stress. Total P and chemical oxygen demand (COD) in effluent showed no significant changes under 0.5-50 mg L-1 PS MPs stress within 32 cycle treatment periods of SBR, while 0.5 mg L-1 PS MPs affected the N cycling process. PS MPs (0.5-50 mg L-1) promoted the richness and diversity of microbial community in SBR, and the denitrification process was exuberant. PS MPs with a low dosage (0.5-5 mg L-1) enhanced secretion of extracellular polymeric substances and promoted expression levels of functional genes related to C fixation, C degradation, P cycling, and N cycling. Simultaneously, aac(3)-II, blaTEM-1, and tetW increased by 27.13%, 38.36%, and 9.57% under low dosages of PS MPs stress; more importantly, the total absolute abundance of intI1 nearly doubled. 78.4% of these P-, N-, and C-related functional genes were positively correlated with intI1, thus favoring transmission of ARGs. This study firstly disclosed the underlying correlations between functional modules of WWTPs and spread of ARGs under MPs stress.

Size-Selective Suzuki-Miyaura Coupling Reaction over Ultrafine Pd Nanocatalysts in a Water-Stable Indium-Organic Framework.

Metal nanoparticles stabilized by crystalline metal-organic frameworks (MOFs) are highly promising for green heterogeneous catalysis. In this work, in situ formed ultrafine Pd nanocatalysts with an average size of 3.14 nm have been successfully immobilized into the mesopores or defects of a water-stable indium-based MOF by the double-solvent method and subsequent reduction. Significantly, the obtained Pd@InOF-1 displays an obvious and satisfactory size-selective effect in the Suzuki-Miyaura coupling reaction between arylboronic acids and aryl bromides. On the basis of the synergistic effect, microporous InOF-1 nanorods afford a confined space for improving the selectivity of target products while Pd nanoparticles endow abundant active sites for catalysis. Herein, choosing the smallest size reactant with only one benzene ring gives the highest isolated yield of 90%, and if the size is larger, the yield is obviously reduced or even the target product could not be collected. Looking forward, this demonstrated study not only assembles a well-designed Pd@MOF composite with unique micro-nanostructures but also delivers an impressive option for cross-coupling reaction, which has implications for the further development of MOF hybrids for sustainable applications.

Diagnostic Value and Application of Prenatal MRI and Ultrasound in Fetal Cleft Lip and Palate.

Objective: The purpose was to explore the diagnostic value and application of prenatal magnetic resonance imaging (MRI) and ultrasound (US) in fetal cleft lip and palate. Methods: From January 2018 to December 2019, 39 pregnant women without normal fetal maxillofacial structure or with fetal maxillofacial deformity under US examination in our hospital were selected as the study subjects. Not knowing the clinical data of the pregnant women, MRI and US physicians performed diagnostic analysis on the MRI or US images of all the study subjects and analyzed the results of prenatal MRI and US diagnosis and postpartum follow-up to compare the diagnostic efficacy and confidence of MRI and US. Results: The follow-up found that there were 20 cases of cleft lip, 15 cases of cheilopalatognathus, 3 cases of cleft palate, and 1 case of unilateral cleft lip with alveolar cleft, with a total of 39 cases having cleft lip and palate deformity. MRI and US had the same efficacy in the diagnosis of cleft lip. As for cleft palates, the diagnostic accuracy of MRI (94.87%) was significantly better than that of US (48.72%, P < 0.001). The diagnostic confidence of fetal cleft lip and palate by MRI (89.73%) was significantly better than that of US (43.59%, P < 0.001). The AUC of US (0.597) was significantly less than that of MRI (0.940), indicating that the diagnostic accuracy of US was not as good as that of MRI (P < 0.05). The sensitivity and 1 - specificity of MRI were significantly higher than those of US. Conclusion: MRI is more accurate than US in the diagnosis of fetal cleft lip and palate, and MRI can be the preferred method for prenatal detection of cleft lip and palate, thus providing more accurate opinions and information for perinatal pregnant women.

An air quality index prediction model based on CNN-ILSTM.

Air quality index (AQI) is an essential measure of air pollution evaluation, which describes the air pollution degree and its impact on health, so the accurate prediction of AQI is significant. This paper presents an AQI prediction model based on Convolution Neural Networks (CNN) and Improved Long Short-Term Memory (ILSTM), named CNN-ILSTM. ILSTM deletes the output gate in LSTM and improves its input gate and forget gate, and introduces a Conversion Information Module (CIM) to prevent supersaturation in the learning process. ILSTM realizes efficient learning of historical data, improves prediction accuracy, and reduces the training time. CNN extracts the eigenvalues of input data effectively. This paper uses air quality data from 00:00 on January 1, 2017, to 23:00 on June 30, 2021, in Shijiazhuang City, Hebei Province, China, as experimental data sets, and compares this model with eight prediction models: SVR, RFR, MLP, LSTM, GRU, ILSTM, CNN-LSTM, and CNN-GRU to prove the validity and accuracy of CNN-ILSTM prediction model. The experimental results show the MAE of CNN-ILSTM is 8.4134, MSE is 202.1923, R2 is 0.9601, and the training time is 85.3 s. In this experiment, the performance of this model performs better than other models.

Leonurine attenuates cisplatin nephrotoxicity by suppressing the NLRP3 inflammasome, mitochondrial dysfunction, and endoplasmic reticulum stress.

PURPOSE: Cisplatin has been widely accepted as an effective chemotherapy drug with various side effects, including nephrotoxicity. The mechanisms of cisplatin-induced acute kidney injury (AKI) are complex, and there are limited renoprotective approaches. Leonurine is the main active compound of a Chinese herb and has recently been reported to have a protective effect on the kidneys. This study aimed to verify the renoprotective effect of leonurine in attenuating cisplatin-induced AKI and explore the potential associated mechanisms. METHODS: C57BL/6 mice were divided into four groups (Sham, Cisplatin, Leonurine, and Cisplatin + Leonurine). Mice in the leonurine-treated groups were pretreated with a daily intraperitoneal injection of 25 mg/kg leonurine. AKI was induced by injecting cisplatin once intraperitoneally at 20 mg/kg body weight. Mice were killed on day 5. Kidney injury was assessed using a serum biochemical and histological assay. Apoptosis was evaluated using a terminal deoxyribonucleotide transferase-mediated dUTP nick-end labeling (TUNEL) staining assay and Western blot. Antioxidant enzymes were detected using commercial kits. The improvement in inflammasome activation, mitochondrial dysfunction, and endoplasmic reticulum stress (ERS) were assessed by polymerase chain reaction (PCR) and Western blot, respectively. RESULTS: Leonurine treatment improved kidney function by preventing renal tubular injury and apoptosis. Expression of nucleotide-binding leucine-rich repeat and pyrin domain containing protein 3 (NLRP3) inflammasome components and inflammatory cytokines, mitochondrial dysfunction, and ERS were all alleviated by leonurine. CONCLUSION: The results indicate that leonurine plays a protective role in cisplatin-induced AKI and may represent an effective multi-targeted intervention strategy.

Inhibition of miR-1298-5p attenuates sepsis lung injury by targeting SOCS6.

Sepsis is one of the leading causes of morbidity and mortality and a major cause of acute lung injury (ALI). carried by exosomes play a role in a variety of diseases. However,there are not many studies of exosomal miRNAs in sepsis and sepsis lung injury.miR-1298-5p and suppressor of cytokine signaling 6 (SOCS6) were silenced or overexpressed in human bronchial epithelial cells (BEAS-2B). PKH-67 Dye was used to trace exosome endocytosis. Cell permeability was evaluated by measuring trans-epithelial electrical resistance (TEER) and FITC dextran flux. ELISA kits were used for cytokine detection. Quantitative RT-PCR and western blots were used to evaluate gene expression. miR-1298-5p was elevated in exosomes from patients with sepsis lung injury (Sepsis_exo). Treatment of BEAS-2B cells using Sepsis_exo significantly inhibited cell proliferation, and induced cell permeability and inflammatory response. miR-1298-5p directly targeted SOCS6. Overexpressing SOCS6 reversed miR-1298-5p-induced cell permeability and inflammatory response. Inhibition of STAT3 blocked SOCS6-silencing caused significant increase of cell permeability and inflammation. Exosomes isolated from patients of sepsis lung injury increased cell permeability and inflammatory response in BEAS-2B cells through exosomal miR-1298-5p which targeted SOCS6 via STAT3 pathway. The findings highlight the importance of miR-1298-5p/SOCS6/STAT3 axis in sepsis lung injury and provide new insights into therapeutic strategies for sepsis lung injury.

The Serum Level of IL-1B Correlates with the Activity of Chronic Pulmonary Aspergillosis.

Background: Until now, there have been no objective criteria to determine the activity of chronic pulmonary aspergillosis (CPA). This study aims to analyze the correlation between serum level of IL-1B and the activity of CPA and to determine whether serum IL-1B could be used to assess the activity of CPA. Methods: A total of 469 newly diagnosed CPA patients were enrolled. Correlation analysis in the whole subjects showed that only IL-1B level was associated with the activity of CPA. Then, 381 cases with factors significantly affecting IL-1B expression was excluded through multiple linear regression; the remaining 88 patients were divided into high IL-1B group and low IL-1B group, according to the median value of serum IL-1B, for subgroup analysis. A retrospective comparative analysis was subsequently performed between the two groups, including the clinical manifestation, microbiology and laboratory tests results, and imaging findings. We further investigated the relationship between IL-1B levels and CT characteristic which acted as the indicator of CPA activity, as well as changes in IL-1B level before and after surgery. Results: For all patients, correlation analysis revealed that IL-1B level correlated with both cavitary diameter (P=0.035) and aspergilloma size (P<0.047) but not with the thickness of the cavity (P=0.479). In subgroup comparative analysis, CT characteristics suggested that high activity of CPA, such as cavitary (27/44 vs 13/44, P=0.003) and aspergilloma lesions (25/44 vs. 11/44, P<0.002), were more frequently found in high IL-1B group. The cavity diameter (P<0.001), aspergilloma size (P=0.006), and cavity wall thickness (P=0.023) were significantly different between the two groups. When Spearman correlation analysis was performed once again in subgroup, an even stronger relationship of serum IL-1B with the cavity diameter (Rs=0.501, P=0.002) and aspergilloma size (Rs=0.615, P=0.001) was observed. Interestingly, a significant reduction of IL-1B level was observed after successful resection of CPA lesions. Conclusion: Higher level of serum IL-1B is associated with more severe cavitary and aspergilloma lesions, which are indicative of more active CPA. In addition, IL-1B level reduced accordingly after lesion resection. Measuring IL-1B level therefore could be served as a convenient method to monitor the activity of CPA and be a potential predictive/prognostic marker for treatment response.

Dimethyl phthalate elimination from micro-polluted source water by surface discharge plasma: Performance, active species roles and mechanisms.

Plasticizer pollution brought huge risks to ecological environment and human health. Surface discharge plasma (SDP) was employed to eliminate plasticizer in natural water, with dimethyl phthalate (DMP) as a typical plasticizer. Experimental results showed that DMP degradation efficiency reached 82.8% within 60 min's SDP treatment, and the elimination process fitted well the first-order kinetic model. Low initial DMP concentration, alkaline condition, and low natural organic matter content were all conducive for DMP degradation. The contributions of OH radical and O2- to DMP elimination were 91.9% and 78.1%, respectively. Total organic carbon (TOC), UV-vis spectroscopy, and atomic force microscopy analysis demonstrated that DMP molecular structure was destroyed after the SDP treatment, and some small molecular fractions were generated. Approximately 47.8% of TOC and 73.5% of COD were eliminated after 60 min's SDP treatment. Phthalic acid monomethyl ester, phthalic acid, o-phthalic anhydride, acetic acid, formic acid, and oxalic acid were detected as the byproducts. Carbon balance analysis among these intermediates showed that total carbon content was approximately 4.64 × 10-2 mmol before treatment, and it was 4.578 × 10-2 mmol after treatment, suggesting that some C-containing intermediates still existed but not detected. DMP degradation pathways in the SDP system were proposed.

Novel Cu(II)-EDTA Decomplexation by Discharge Plasma Oxidation and Coupled Cu Removal by Alkaline Precipitation: Underneath Mechanisms.

Strong complexation between heavy metals and organic complexing agents makes the heavy metals difficult to be removed by classical chemical precipitation. In this study, a novel decomplexation method was developed using discharge plasma oxidation, which was followed by alkaline precipitation to treat water containing heavy metal-organic complex, that is, Cu-ethylenediaminetetraacetic acid (Cu-EDTA). The decomplexation efficiency of Cu complex reached up to nearly 100% after 60 min's oxidation by discharge plasma, which was accompanied by 82.1% of total organic carbon removal and energy efficiency of 0.62 g kWh-1. Presence of free Cu2+ favored Cu-EDTA decomplexation, whereas the presence of excessive EDTA depressed this process. Cu-EDTA decomplexation was mainly driven by the produced 1O2, O2•-, O3, and •OH by discharge plasma. Cu-EDTA decomplexation process was characterized by UV-vis, ATR-FTIR, total organic carbon, and three-dimensional fluorescence diagnosis. The main intermediates including Cu-EDDA, Cu-IDA, Cu-NTA, small organic acids, NH4+, and NO3- were identified, accompanied by Cu2+ releasing. The followed precipitation process removed 78.1% of Cu2+, and Cu-associated precipitates included CuCO3, Cu2CO3(OH)2, CuO, and Cu(OH)2. A possible pathway of Cu complex decomplexation and Cu2+ removal in such a system was proposed.

Chemical conversion of human lung fibroblasts into neuronal cells.

It has been previously reported that human embryonic fibroblasts and mouse embryonic fibroblasts can be converted into neuronal cells using chemical agents, along with forced expression specific transcriptional factors. However, the materials required for reprogramming in these approaches presents major technical difficulties and safety concerns. The current study investigated whether a cocktail of small molecules can convert human lung fibroblast cells into neurons. The small molecules valproic acid, CHIR99021, DMH1, Repsox, forskolin, Y­27632 and SP600125 (VCHRFYS) were used to induce MRC­5 cells into neuronal cells in vitro. Neuronal markers were analyzed by immunofluorescence staining. The gene profiles were analyzed by reverse transcription­quantitative polymerase chain reaction. MRC­5 is a human lung fibroblast cell line derived from normal lung tissue of a 14­week­old male fetus. The results of the current study demonstrated that MRC­5 fibroblasts can be directly converted into neuronal cells using a cocktail of seven small molecules (VCHRFYS), with a yield of ~90% Tuj1­positive cells after 7 days of induction. Following a further maturation period, these chemically-induced neurons possessed neuronal morphology and expressed multiple neuron­specific genes. In conclusion, a cocktail of small molecules that can convert fibroblasts MRC­5 cells into functional neurons without the exogenous genetic factors was identified, which has the potential to be useful in neurological disease therapy.

Alleviation of adverse effects of drought stress on wheat seed germination using atmospheric dielectric barrier discharge plasma treatment.

Atmospheric dielectric barrier discharge (DBD) was attempted to improve the resistance of wheat seed to drought stress. Effects of DBD plasma on wheat seed germination, seedling growth, osmotic-adjustment products, lipid peroxidation, reactive oxygen species (ROS), antioxidant enzyme activity, abscisic acid, and drought resistant related genes expression under drought stress were investigated. The changes of the wheat seed coat before and after the DBD plasma treatment were explored. Experimental results showed that the DBD plasma treatment could alleviate the adverse effects of drought stress on wheat seed germination and seedling growth; the germination potential and germination rate increased by 27.2% and 27.6%, and the root length and shoot length of the wheat seedlings also increased. Proline and soluble sugar levels under drought stress were improved after the DBD plasma treatment, whereas the malondialdehyde content decreased. ROS contents under drought stress were reduced after the DBD plasma treatment, whereas the activities of superoxide dismutase, catalase, and peroxidase were promoted. DBD plasma treatment promoted abscisic acid generation in wheat seedlings, and it also regulated functional gene LEA1 and stimulated regulation genes SnRK2 and P5CS to resist drought stress. Etching effect and surface modification occurred on the seed coat after the DBD plasma treatment.

Enhanced removal of humic acid from micro-polluted source water in a surface discharge plasma system coupled with activated carbon.

Surface discharge plasma (SDP) combined with activated carbon (AC) was employed to eliminate dissolved organic matter from micro-polluted source water, with humic acid (HA) as the model pollutant. Synergistic effect on HA removal was observed in the SDP-AC system; HA removal efficiency reached 60.9% within 5-min treatment in the SDP-AC system with 5.0 g AC addition, whereas 16.7 and 17.4% of HA were removed in sole SDP system and AC adsorption, respectively. Scanning electron microscope and Boehm titration analysis showed that chemical reactions between active species and functional groups of AC occurred. The existence of isopropanol or benzoquinone exhibited inhibitive effects on HA removal in the SDP system, while these inhibitive effects were weakened in the SDP-AC system. The influences of AC on ozone equivalent concentration and H2O2 concentration were evaluated, and there were approximately 39 and 20% decline in ozone equivalent concentration and H2O2 concentration within 6-min treatment in the SDP-AC system, respectively, compared with those in the sole SDP system. Dissolved organic carbon, specific ultraviolet absorbance, and UV absorption ratios analysis demonstrated that the SDP treatment destroyed the chromophoric groups, double bonds, and aromatic structure of HA molecules, and these destructive actions were strengthened by AC.

Endobronchial aspergilloma: A case report and literature review.

The present study aimed to investigate the clinical and radiological characteristics in addition to the bronchoscopic appearance in patients with endobronchial aspergilloma (EBA). Clinical and radiological characteristics were analyzed alongside the bronchoscopic appearance in 17 patients with EBA diagnosed by bronchoscopy with histological examination. The present study assessed the relevant literature and 13 males and 4 females were included in the comparison, with a median age of 59. Associated diseases included 8 previous diagnoses of pulmonary tuberculosis (47.6%), 4 previous diagnoses lung cancer (23.5%), 1 pulmonary resection (5.9%) and 1 bronchial foreign body (5.9%). The primary symptom was hemoptysis (9/17, 53%). Chest computed tomography (CT) indicated a markedly higher incidence of aspergillosis lesion in the left lung (13/17; 76.5%) compared with the right lung (4/17; 23.5%). CT manifestation included space occupying disease in 10 patients (58.8%), aspergilloma in 3 patients (17.6%), pneumonic consolidation in 2 patients (11.8%) and ground glass opacity in 1 patient (5.9%). Bronchoscopy examination identified masses in all 17 patients' bronchial lumen and 15 patients had endobronchial obstruction by necrotic material. The case presented in the current study demonstrated the merits of combining bronchosopic intervention with voriconazole. The dominant symptom of EBA was hemoptysis. Chest CT demonstrated that aspergillosis lesions were more frequently identified in the left lung compared with the right. EBA often occurs in individuals with underlying lung diseases, which cause lumen structural change or bronchial obstruction. EBA may be clearly diagnosed by bronchoscopy biopsy, although the potential for a co-exististing tumor requires consideration. Bronchoscopic intervention and anti-fungal therapy may have an advantage in the effective treatment of patients with EBA.

Organic acids enhanced decoloration of azo dye in gas phase surface discharge plasma system.

A gas phase surface discharge plasma combined with organic acids system was developed to enhance active species mass transfer and dye-containing wastewater treatment efficacy, with Acid Orange II (AO7) as the model pollutant. The effects of discharge voltage and various organic acid additives (acetic acid, lactic acid and nonoic acid) on AO7 decoloration efficiency were evaluated. The experimental results showed that an AO7 decoloration efficiency of approximately 69.0% was obtained within 4 min of discharge plasma treatment without organic acid addition, which was improved to 82.8%, 83.5% and 88.6% within the same treatment time with the addition of acetic acid, lactic acid and nonoic acid, respectively. The enhancement effects on AO7 decoloration efficiency could be attributed to the decrease in aqueous surface tension, improvement in bubble distribution and shape, and increase in ozone equivalent concentration. The AO7 wastewater was biodegradable after discharge plasma treatment with the addition of organic acid. AO7 decomposition intermediates were analyzed by UV-vis spectrometry and GC-MS; 2-naphthol, 1,4-benzoquinone, phthalic anhydride, coumarin, 1,2-naphthoquinone, and 2-formyl-benzoic acid were detected. A possible pathway for AO7 decomposition in this system was proposed.

Evaluation of the potentials of humic acid removal in water by gas phase surface discharge plasma.

Degradation of humic acid (HA), a predominant type of natural organic matter in ground water and surface waters, was conducted using a gas phase surface discharge plasma system. HA standard and two surface waters (Wetland, and Weihe River) were selected as the targets. The experimental results showed that about 90.9% of standard HA was smoothly removed within 40 min's discharge plasma treatment at discharge voltage 23.0 kV, and the removal process fitted the first-order kinetic model. Roles of some active species in HA removal were studied by evaluating the effects of solution pH and OH radical scavenger; and the results presented that O3 and OH radical played significant roles in HA removal. Scanning electron microscope (SEM) and FTIR analysis showed that HA surface topography and molecular structure were changed during discharge plasma process. The mineralization of HA was analyzed by UV-Vis spectrum, dissolved organic carbon (DOC), specific UV absorbance (SUVA), UV absorption ratios, and excitation-emission matrix (EEM) fluorescence. The formation of disinfection by-products during HA sample chlorination was also identified, and CHCl3 was detected as the main disinfection by-product, but discharge plasma treatment could suppress its formation to a certain extent. In addition, approximately 82.3% and 67.9% of UV254 were removed for the Weihe River water and the Wetland water after 40 min of discharge plasma treatment.

[Effects of blood glucose control on glucose variability and clinical outcomes in patients with severe acute pancreatitis in intensive care unit].

OBJECTIVE: To explore the effects of blood glucose control on glucose variability and clinical outcomes in patients with severe acute pancreatitis in intensive care unit (ICU). METHODS: A total of 72 ICU patients with severe acute pancreatitis were recruited and divided randomly into observation and control groups (n = 36 each). Both groups were treated conventionally. And the observation group achieved stable blood glucose at 6.1-8.3 mmol/L with intensive glucose control. The length of ICU and hospital stays, ICU mortality rate, transit operative rate, concurrent infection rate, admission blood glucose, glycosylated hemoglobin, mean insulin dose, mean blood glucose, blood glucose value standard deviation (GLUSD), glycemic liability index (GLUGLI) and mean amplitude of glycemic excursion (GLUMAGE) of two groups were compared. At the same time, the relationship between blood glucose variability, ICU mortality rate and its predictive value were analyzed by correlation analysis and receiver operating characteristic curve (ROC). RESULTS: The lengths of ICU and hospital stays of observation group were all significantly less than those of the control group [(11.7 ± 9.9) vs (15.9 ± 8.02) days, (21.8 ± 10.8) vs (28.2 ± 12.7) days, P < 0.05]. In observation group, the rates of pulmonary infection (27.78%) and hematogenous infection (5.56%) were all significantly lower than those of control group (72.22%, 38.89%, P < 0.05). The values of mean blood glucose value and GLUSD of observation group were significantly lower than those of control group [(7.4 ± 1.1) vs (9.6 ± 1.2), (1.8 ± 1.0) vs (2.5 ± 1.3) mmol/L]. The differences were statistically significant (P < 0.05). While the dose of insulin [(70.2 ± 47.6) vs (34.4 ± 38.6) U/d] was significantly higher than that of control group (P < 0.05). Bivariate correlation analysis showed that ICU mortality rate was positively correlated with GLUGLI (r = 0.368, P < 0.05). ROC curve analysis showed that, AUC of GLUGLI was 0.748 and 95% CI 0.551-0.965 (P < 0.05). CONCLUSION: Intensive glucose control in patients with severe acute pancreatitis helps reduce the blood sugar fluctuations, lower the risks of infectious complications and promote the patient rehabilitation. And GLUGLI is positively correlated with ICU mortality rate. It has good predictive values.

Evaluation of the potential of p-nitrophenol degradation in dredged sediment by pulsed discharge plasma.

Hazardous pollutants in dredged sediment pose great threats to ecological environment and human health. A novel approach, named pulsed discharge plasma (PDP), was employed for the degradation of p-nitrophenol (PNP) in dredged sediment. Experimental results showed that 92.9% of PNP in sediment was smoothly removed in 60 min, and the degradation process fitted the first-order kinetic model. Roles of some active species in PNP degradation in sediment were studied by various gas plasmas, OH radical scavenger, hydrated electron scavenger and O2(·-) scavenger; and the results presented that O3, OH radical, eaq(-) and O2(·-) all played significant roles in PNP removal, and eaq(-) and O2(·-) mainly participated in other oxidising active species formation. FTIR analysis showed that PNP molecular structure was destroyed after PDP treatment. The main degradation intermediates were identified as hydroquinone, benzoquinone, phenol, acetic acid, NO2(-) and NO3(-). PNP degradation pathway in dredged sediment was proposed. It is expected to contribute to an alternative for sediment remediation by pulse discharge plasma.

The strategies for treating primary hepatocellular carcinoma with portal vein tumor thrombus.

PURPOSE: To further improve the effectiveness and prognosis of primary hepatocellular carcinoma (HCC) with portal vein tumor thrombus (PVTT), the current status of treatment for HCC with PVTT was reviewed. METHODS: A Medline search was undertaken to identify articles using the keywords "HCC", "PVTT" and "therapy". Additional papers were identified by a manual search of the references from the key articles. RESULTS: PVTT, as a common complication of HCC, was divided into type I ∼ IV. The therapeutic approach is mainly composed of five types: surgical resection, regional interventional therapy, radiotherapy, combination therapy, targeted therapy. All of these therapeutic approaches were separately evaluated in detail. CONCLUSIONS: For those resectable tumors, the better choice for treatment of HCC with PVTT should be hepatectomy and removal of PVTT. For those unresectable tumors, TACE (especially the super-selective TACE) has been the preferred palliative treatment, the other regional interventional therapy and/or radiotherapy could improve the therapeutic effects. The multidisciplinary treatments may further improve the quality of life and prolong the survival period for the HCC patients associated with PVTT.