Expression levels of nitrogen assimilation-related genes, physiological responses, and morphological adaptations of three indica rice (Oryza sativa L. ssp. indica) genotypes subjected to nitrogen starvation conditions.

Nitrogen (N) is an essential nutrient available to the plants in form of nitrate and ammonium. It is a macronutrient important for the plant growth and development, especially in cereal crops, which consume it for the production of amino acids, proteins/enzymes, nucleic acids, cell wall complexes, plant hormones, and vitamins. In rice production, 17 kg N uptake is required to produce 1 ton of rice. Considering this, many techniques have been developed to evaluate leaf greenness or SPAD value for assessing the amount of N application in the rice cultivar to maximize the grain yield. The aim of the present study was to investigate the morpho-physiological characteristics and relative expression level of N assimilation in three different rice genotypes (MT2, RD31, KDML105) under 1.00 × (full N), 0.50 × , 0.25 × (N depletion), and 0.00 × (N deficiency) at seedling stage and the morpho-physiological traits and the grain yield attributes under 1.00 × (full N) and 0.25 × (N depletion) were compared. Leaf chlorosis and growth inhibition in rice seedlings under N deficiency were evidently observed. Shoot height, number of leaves, shoot fresh weight, shoot dry weight, and root fresh weight in KDML105 under N deficiency were decreased by 27.65%, 42.11%, 65.44%, 47.90%, and 54.09% over the control (full N). Likewise, leaf greenness was lowest in KDML105 under N deficiency (78.57% reduction over the full N), leading to low photosynthetic abilities. In addition, expression of nitrogen assimilation-related genes, OsNR1, OsGln1;1, and OsGln2, in KDML105 under N depletion were increased within 3 h and then declined after the long incubation period, whereas those were unchanged in cvs. MT2 and RD31. Similarly, relative expression level of OsNADH-GOGAT, OsFd-GOGAT, and OsAspAt1 in KDML105 was peaked when subjected to 0.50 × N for 6 h and then declined after the long incubation period. Moreover, overall growth characters and physiological changes in cv. RD31 at vegetative stage under 0.25 × N were retained better than those in cvs. KDML105 and MT2, resulting in high yield at the harvesting process. In summary, N assimilated-related genes in rice seedlings under N depletion were rapidly regulated within 3-6 h, especially cv. KDML105 and MT2, then downregulated, resulting in physiological changes, growth inhibition, and yield reduction.

Bioactive Compounds Produced in Leaves of Mulberry (Morus alba L.) Transplants under Modified Environments of Root and Aerial Zones.

Different shoot/root micro-environments were investigated for growth performances and nutraceutical compounds in leaves of mulberry (Morus alba L.) transplants. Single-node segments were taken from seedling-grown pots of three cultivars: Myanmar large leaf (MLL), Myanmar medium leaf (MML), and C14. Transplant production was compared in soil, vermiculite (V), or the dynamic root floating technique (DRFT). The highest survival percentage of the transplants was obtained from V-system, and MLL showed a higher shoot/root formation over two tested cultivars. The MLL transplants grown in V-system under white LED light (445 and 554 nm) at 200 µmol·m-2·s-1 gave a fresh weight with superior qualified transplants compared to other treatments. The bioactive compounds in leaves of MLL, MML, and C14 were analyzed using GC-MS after incubation with different LED spectra. Ethanol extracts of the leaves revealed that more than 50% of the bioactive compounds were fatty acids and conjugates and varied according to spectra and cultivar. Blue LED light (445 nm) induced the production of total phenolics, whereas white LED light favored the production of total proteins, soluble sugar, and biomass. The modified environments at the root and aerial zones significantly influenced the growth and biochemical parameters of transplants, and this applied technique can elevate useful functional ingredients of mulberry leaves.

Stable and reproducible expression of bacterial ipt gene under the control of SAM-specific promoter (pKNOX1) with interference of developmental patterns in transgenic Peperomia pellucida plants.

Reproducible and stable transgene expression is an important goal in plant basic research and applications. Hence, we report the first stable expression of bacterial transgenes in medicinal plant, Peperomia pellucida (L.) Kunth. Two key elements relevant to the dynamic expression of the bacterial cytokinin biosynthesis gene, ipt (isopentenyltransferase) were examined. First, by designing a specific expression cassette driven by a tissue-specific promoter for the required levels of gene expression in the particular function of development, and second by using P. pellucida as a model plant due to its short developmental cycle that supported expedient tracking of transgene expression in the progeny. Transgenic frequencies of ipt gene obtained from different expression cassettes of pKNOX1 for tissue-specific promoter in shoot apical meristem were compared with the cauliflower mosaic virus (CaMV35S) promoter (p35S), a constitutive promoter investigated for T3 generation. It was clearly shown that transgenic plants with pKNOX1 showed percentage of survivals in T3 at about 2.2 folds more than those of p35S-transgenic. Transgenic P. pellucida under controllable expression of pKNOX1 showed increased leaf and seed size with a high percentage of fertile seed, whereas transgenic plants with p35S showed phenotypic features of bushy and small leaves, sterile pollen and lower reproductive fitness. Quantitative examination of ipt-positive gene expression in T3 generation of transformants with pKNOX1 were 100% (line k-14) and 50% (line k-20), while 33.3% was observed in transgenic line c-11 with p35S. Interestingly, the endogenous cytokinin biosynthesis gene (ipt3) was significantly upregulated (2-3 folds higher) in pKNOX1-transformants. The overall relative mRNA expression of bacterial ipt gene and overproducing of cytokinin contents (t-ZR and 2-iP) detected in p35S-transformants caused abnormality and low percentages of transgene reproducible Interestingly, pKNOX1-transgenic plants tended to maintain chlorophyll contents 4-5 folds and extending the developmental cycle to 12.4 weeks (wk), which was 2 folds more than wildtype (5.8 wk) and p35S-transformants (7.4 wk). The promotor effect on stable and reproducible transgene-expressions demonstrated prominent features of P. pellucida and also empowered further omics studies.

A short-term cooling of root-zone temperature increases bioactive compounds in baby leaf Amaranthus tricolor L.

Leafy vegetables that are offered as seedling leaves with petioles are referred to as baby leaf vegetables. One of the most nutritious baby leaves, amaranth (Amaranthus tricolor L.), contains several bioactive compounds and nutrients. Here, we investigated the growth and quality of baby leaf amaranth using a variety of short-term cooling root-zone temperatures (RZT; 5, 10, 15, and 20°C), periods (1, 3, 5, and 7 days), and combinations thereof. We observed that exposing amaranth seedlings to RZT treatments at 5 and 10°C for 1-3 days increased the antioxidant capacity and the concentrations of bioactive compounds, such as betalain, anthocyanin, phenolic, flavonoid, and ascorbic acid; however, extending the treatment period to 7 days decreased them and adversely affected growth. For RZT treatments at 20°C, leaf photosynthetic pigments, bioactive compounds, nutrients, and antioxidant capacity increased gradually as the treatment period was extended to 7 days. The integration of RZTs at 5 and 10°C for one day preceded or followed by an RZT treatment at 20°C for 2 days had varied effects on the growth and quality of amaranth leaves. After one day of RZT treatment at 5°C followed by 2 days of RZT treatment at 20°C, the highest concentrations of bioactive compounds, nutrients, and antioxidant capacity were 1.4-3.0, 1.7, and 1.7 times higher, respectively, than those of the control, and growth was not impaired. The short-term cooling RZT treatments under controlled environments were demonstrated to be adequate conditions for the improvement of target bioactive compounds in amaranth baby leaf without causing leaf abnormality or growth impairment.

Induction of dwarf and early flowering phenotypes in Tricyrtis sp. by ectopic expression of LEAFY from Arabidopsis thaliana.

LEAFY (LFY), which encodes a plant-specific transcription factor, plays an important role in the transition from vegetative to reproductive development. Ectopic expression of LFY has been reported to induce dwarfism and early flowering in some model plants. In order to examine the possibility of using LFY for molecular breeding of ornamental plants, we produced and characterized transgenic plants ectopically expressing LFY from Arabidopsis thaliana (AtLFY) in the liliaceous ornamental plant Tricyrtis sp. Nine independent transgenic plants have been obtained, and all of them exhibited dwarf phenotypes compared with the vector control. These transgenic plants could be classified into three types according to the degree of dwarfism: one showed an extreamly dwarf phenotype with smaller leaves (Type I); two showed moderately dwarf phenotypes (Type II); and six showed slightly dwarf phenotypes (Type III). All of Type I, Type II and Type III transgenic plants produced flower buds 1-3 weeks earlier than the vector control. Vector control and Type III transgenic plants produced 1-4 apical flower buds, whereas Type I and Type II transgenic plants produced only a single apical flower bud. Type I and Type II transgenic plants often produced non-fully-opened flowers. Quantitative real-time reverse transcription-polymerase chain reaction analysis showed that the AtLFY expression level generally correlated with the degree of dwarfism. These results indicate that morphological alterations observed in the transgenic plants was induced by ectopic expression of AtLFY. Lower levels of ectopic expression of LFY may be valuable for producing dwarf and early flowering ornamental plants.

Enhancement of bioactive compounds in baby leaf Amaranthus tricolor L. using short-term application of UV-B irradiation.

Baby-leaf vegetables are a trade name for leafy vegetables sold as leaves with petioles at the seedling stage. Amaranth (Amaranthus tricolor L.) is a nutritious baby-leaf vegetable containing many bioactive compounds. The effects of short-term ultraviolet B (UV-B) treatments on the growth and quality of baby leaf amaranth were studied, including the conditions of a 24-h recovery period after irradiation, and different irradiation intensities (3.0-9.0 W m-2), irradiation periods (4-16 h), and cumulative energies (130-170 kJ m-2). A recovery period experiment was conducted to observe the changes in the growth and quality of leaves at 0 and 24 h after UV-B irradiation. The results showed that the concentrations of phenolic compounds, flavonoids, anthocyanin, and ascorbic acid in the leaves, as well as the leaf antioxidant capacity increased 24 h after UV-B irradiation. Increases in target compound concentrations and antioxidant capacity without negative growth and appearance effects were observed in leaves irradiated with UV-B at 3, 6, and 9 W m-2 for irradiation periods of 12 and 16, 8 and 12, and 4 h, respectively. The highest bioactive compound concentration was found in leaves irradiated with UV-B at 6 W m-2 for 7 h (cumulative energy: 150 kJ m-2). It was concluded that UV-B irradiation at 6 W m-2 with a cumulative energy of 150 kJ m-2 and a 24 h post-irradiation recovery period could be an appropriate treatment to increase bioactive compounds in baby leaf amaranth without causing appearance abnormalities.

Variation in terpenoids in leaves of Artemisia annua grown under different LED spectra resulting in diverse antimalarial activities against Plasmodium falciparum.

BACKGROUND: Productivities of bioactive compounds in high-value herbs and medicinal plants are often compromised by uncontrollable environmental parameters. Recent advances in the development of plant factories with artificial lighting (PFAL) have led to improved qualitative and/or quantitative production of bioactive compounds in several medicinal plants. However, information concerning the effect of light qualities on plant pharmaceutical properties is limited. The influence of three different light-emitting diode (LED) spectra on leaf fresh weight (FW), bioactive compound production and bioactivity of Artemisia annua L. against the malarial parasite Plasmodium falciparum NF54 was investigated. Correlation between the A. annua metabolites and antimalarial activity of light-treated plant extracts were also determined. RESULTS: Artemisia annua plants grown under white and blue spectra that intersected at 445 nm exhibited higher leaf FW and increased amounts of artemisinin and artemisinic acid, with enhanced production of several terpenoids displaying a variety of pharmacological activities. Conversely, the red spectrum led to diminished production of bioactive compounds and a distinct metabolite profile compared with other wavelengths. Crude extracts obtained from white and blue spectral treatments exhibited 2 times higher anti-Plasmodium falciparum activity than those subjected to the red treatment. Highest bioactivity was 4 times greater than those obtained from greenhouse-grown plants. Hierarchical cluster analysis (HCA) revealed a strong correlation between levels of several terpenoids and antimalarial activity, suggesting that these compounds might be involved in increasing antimalarial activity. CONCLUSIONS: Results demonstrated a strategy to overcome the limitation of A. annua cultivation in Bangkok, Thailand. A specific LED spectrum that operated in a PFAL system promoted the accumulation of some useful phytochemicals in A. annua, leading to increased antimalarial activity. Therefore, the application of PFAL with appropriate light spectra showed promise as an alternative method for industrial production of A. annua or other useful medicinal plants with minimal environmental influence.

A synthetic cytokinin influences the accumulation of leaf soluble sugars and sugar transporters, and enhances the drought adaptability in rice.

Oryza sativa cv. PTT1 (Pathumthani1) was treated with phenyl-urea-based synthetic cytokinin under drought stress. Soluble sugar contents were examined in rice flag leaves at tillering and grain-filling stages. The same leaf samples were used to analyze the differential abundance intensities of proteins related to metabolism and transport of soluble sugars, and the process of senescence. The results showed drought-induced accumulation of hexose sugars (glucose and fructose) in rice flag leaves, which could be corroborated with enhanced accumulation of MST8 under drought stress. On the other hand, cytokinin-treated plants maintained the normal contents of hexose sugar in their flag leaves under drought stress, alike well-watered plants. In the case of sucrose, cytokinin treatment reduced its accumulation at tillering stage, but the results were reversed at the grain-filling stage, where the cytokinin-treated plants maintained significantly higher contents of sucrose under drought stress. Growth stage dependent variations in sucrose contents corroborated with the accumulation of SPS (SPS1, SPS2, and SPS5) proteins, implicated in sucrose biosynthesis. In our study, among the proteins involved in sucrose transport, SUT1 transporter was induced by drought stress at both the growth stages, whereas SUT2 transporter accumulated equally in all the treatments. However, cytokinin treatment reversed the effect of drought on the accumulation of SUT1. Similarly, SWEET5, and SWEET13 proteins, which were induced by drought stress treatment, were inhibited by cytokinin treatment. However, the accumulation SWEET6, SWEET7, and SWEET15 was not influenced by the treatment of cytokinin in the flag leaves of rice. In addition, cytokinin treatment reduced the leaf wilting, enhanced the fresh weight and grain yield, and curtailed the accumulation of proteins involved in drought-induced senescence. In conclusion, the cytokinin treatment had a positive agro-economic impact on the rice plants and provided better drought adaptability.

A Synthetic Cytokinin Improves Photosynthesis in Rice under Drought Stress by Modulating the Abundance of Proteins Related to Stomatal Conductance, Chlorophyll Contents, and Rubisco Activity.

Drought susceptible rice cultivar PTT1 (Pathumthani1) was treated with drought (-72 kPa) and CPPU (N-2-(chloro-4-pyridyl)-N-phenyl urea) @ 5 mg/L at tillering and grain-filling stages. Plants were tested for the effect of synthetic cytokinin on the parameters influencing the process of photosynthesis. Exogenous spray of CPPU improved the stomatal conductance of rice leaves, which was severely reduced by drought. The abundance intensities of proteins, associated with the stomatal conductance (ZEP, NCED4, PYL9, PYL10, ABI5, SnRK4, Phot1, and Phot2), were also in agreement with the positive impact of CPPU on the stomatal conductance under drought stress. Among the photosynthetic pigments, Chl b contents were significantly reduced by drought stress, whereas CPPU treated plants retained the normal contents of Chl b under drought stress. Subsequently, we examined the abundance intensities of chlorophyll synthase and HCR proteins, implicated in the biosynthesis of chlorophyll pigments and the conversion of Chl b to Chl a, respectively. The results indicated a drought-mediated suppression of chlorophyll synthase. However, CPPU treated plants retained normal levels of chlorophyll synthase under drought stress. In addition, drought stress induced HCR proteins, which might be the cause for reduced Chl b contents in drought stressed plants. Further, CPPU treatment helped the plants sustain photosynthesis at a normal rate under drought stress, which was comparable with well-watered plants. The results were further confirmed by examining the abundance intensities of two key proteins, RAF1 and Rubisco activase, implicated in the assembly and activation of Rubisco, respectively. CPPU treatment reversed the drought mediated suppression of these proteins at both of the growth stages of rice under drought stress. Based on the results, it can be suggested that synthetic cytokinins help the plants sustain photosynthesis at a normal rate under drought stress by positively influencing the determinants of photosynthesis at a molecular level.

The Mode of Cytokinin Functions Assisting Plant Adaptations to Osmotic Stresses.

Plants respond to abiotic stresses by activating a specific genetic program that supports survival by developing robust adaptive mechanisms. This leads to accelerated senescence and reduced growth, resulting in negative agro-economic impacts on crop productivity. Cytokinins (CKs) customarily regulate various biological processes in plants, including growth and development. In recent years, cytokinins have been implicated in adaptations to osmotic stresses with improved plant growth and yield. Endogenous CK content under osmotic stresses can be enhanced either by transforming plants with a bacterial isopentenyl transferase (IPT) gene under the control of a stress inducible promoter or by exogenous application of synthetic CKs. CKs counteract osmotic stress-induced premature senescence by redistributing soluble sugars and inhibiting the expression of senescence-associated genes. Elevated CK contents under osmotic stress antagonize abscisic acid (ABA) signaling and ABA mediated responses, delay leaf senescence, reduce reactive oxygen species (ROS) damage and lipid peroxidation, improve plant growth, and ameliorate osmotic stress adaptability in plants.

Comparative proteomics and protein profile related to phenolic compounds and antioxidant activity in germinated Oryza sativa 'KDML105' and Thai brown rice 'Mali Daeng' for better nutritional value.

BACKGROUND: Brown rice (BR) and germinated brown rice (GBR) are considered as prime sources of carbohydrate and bioactive compounds for more than half of the populations worldwide. Several studies have reported on the proteomics of BR and GBR; however, the proteomic profiles related to the synthesis of bioactive compounds are less well documented. In the present study, BR and GBR were used in a comparative analysis of the proteomic and bioactive compound profiles for two famous Thai rice varieties: Khao Dawk Mali 105 (KDML) and Mali Daeng (MD). RESULTS: The proteomes of KDML and MD revealed differences in the expression patterns of proteins after germination. Total phenolic compound content, anthocyanin contents and antioxidant activity of red rice MD was approximately 2.6-, 2.2- and 9.2-fold higher, respectively, compared to that of the white rice KDML. Moreover, GBR of MD showed higher total anthocyanin content and greater antioxidant activity, which is consistent with the increase expression of several proteins involved in the biosynthesis of phenolic compounds and protection against oxidative stress. CONCLUSION: Red rice MD exhibits higher nutrient values compared to white rice KDML and the appropriate germination of brown rice could represent a method for improving health-related benefits. © 2017 Society of Chemical Industry.

A Phenylurea Cytokinin, CPPU, Elevated Reducing Sugar and Correlated to Andrographolide Contents in Leaves of Andrographis paniculata (Burm. F.) Wall. Ex Nees.

Cytokinins are phytohormones that play multiple roles to control plant growth and development. In this study, leaf biomass and the production of andrographolide compounds in a medicinal plant Andrographis paniculata were significantly increased after exogenously treating with the synthetic cytokinin cytokinin-1-(2-chloro-4-pyridyl)-3-phenylurea (CPPU) at 0 (water), 5, or 10 mg L-1 and observed the results for 24 h, 48 h, and 7 days of treatment. It was found that CPPU could significantly enhance new axillary bud formation and further promote branching 4.6-5.6-fold higher, resulting in higher fresh weight (FW) and dry weight (DW) than the control. Application of CPPU at 5 mg L-1 significantly promoted the highest contents of total reducing sugar at 2.5-fold in leaves and at 1.5-fold in roots. Although treatments of CPPU significantly affected the increasing contents of chlorophyll and carotenoid (1.2-1.6-fold), CPPU at 10 mg L-1 slightly caused leaf stress and chlorophyll reduction. Interestingly, 5 mg L-1 CPPU could enhance andrographolide content, an active anti-infectious compound in Andrographis paniculata (2.2-fold higher than the control) that reached the highest content at 24 h after treatment. This study suggested that CPPU should be suitable for field application to promote leaf yields and induce the production of useful pharmaceutical compounds in Andrographis paniculata.

T-DNA insertion alters the terpenoid content composition and bioactivity of transgenic Artemisia annua.

In this study, the interference of T-DNA insertion upon Agrobacterium-mediated transformation on the biochemical expression of the host genome is discussed. Plant extracts of transgenic Artemisia annua L. with or without an overexpressed famesyl pyrophosphate synthase gene have been investigated for their bioactivity and metabolic profile in comparison with wild type A. annua. The highest antimicrobial activity against Staphylococcus aureus, Bacillus subtilis and Candida albicans was observed in the T253 transgenic lines. Moreover, the crude extract from T253 showed higher antimalarial activity against the Plasmodium faciparum K1 strain than those of the others. The terpenoid constituents and antimicrobial properties of the plant samples were grouped by hierarchical clustering analysis. The clustering showed that squalene is a putative compound that might be involved in increasing the bioactivity of the transgenic line. In addition, T253 had a triterpene content that was about twice as great as that of the T253-2 line, which had a higher content of sesquiterpenes. However, both lines were transformed by the same FPS gene. These results suggested that the different bioactive properties observed in each transgenic line may be caused by variations in their terpenoid composition, which is affected by T-DNA insertion at different positions in the host plant.

CPPU elevates photosynthetic abilities, growth performances and yield traits in salt stressed rice (Oryza sativa L. spp. indica) via free proline and sugar accumulation.

Application of N-2-(chloro-4-pyridyl)-N-phenyl urea (CPPU) to salt susceptible cultivar of indica rice (cv. PTT1) effected on free proline and soluble sugar accumulation and regulated the physio-morphological responses when subjected to salt stress condition was firstly demonstrated in this study. Soluble sugars, including sucrose, glucose and fructose, in leaf blade and leaf sheath were enriched in 0.1 mM CPPU pretreated plants subsequently exposed to 16.6 dS m(-1) NaCl for 10 days. In the long period (15 days) salt stress, free proline content in the leaf blade and leaf sheath were evidently peaked to act as osmotic adjustment in the salt-stressed plants. In addition, the photosynthetic pigments, including chlorophyll a, chlorophyll b, total chlorophyll and total carotenoids, were maintained by the functional regulation of soluble sugar and free proline in the cellular levels, thereby leading to higher net photosynthetic rate. Further, the stomatal closure and transpiration rate in CPPU pretreated plants were retained under salt stress, thereby resulting in alleviation of growth performance and yield traits. This study suggested that exogenous application of CPPU may alternatively play effective role to improve the salt tolerant abilities of salt susceptible rice crop.

Overexpression of the gibberellin 2-oxidase gene from Torenia fournieri induces dwarf phenotypes in the liliaceous monocotyledon Tricyrtis sp.

Gibberellins (GAs) are the plant hormones that control many aspects of plant growth and development, including stem elongation. Genes encoding enzymes related to the GA biosynthetic and metabolic pathway have been isolated and characterized in many plant species. Gibberellin 2-oxidase (GA2ox) catalyzes bioactive GAs or their immediate precursors to inactive forms; therefore, playing a direct role in determining the levels of bioactive GAs. In the present study, we produced transgenic plants of the liliaceous monocotyledon Tricyrtis sp. overexpressing the GA2ox gene from the linderniaceous dicotyledon Torenia fournieri (TfGA2ox2). All six transgenic plants exhibited dwarf phenotypes, and they could be classified into two classes according to the degree of dwarfism: three plants were moderately dwarf and three were severely dwarf. All of the transgenic plants had small or no flowers, and smaller, rounder and darker green leaves. Quantitative real-time reverse transcription-polymerase chain reaction (PCR) analysis showed that the TfGA2ox2 expression level generally correlated with the degree of dwarfism. The endogenous levels of bioactive GAs, GA1 and GA4, largely decreased in transgenic plants as shown by liquid chromatography-mass spectrometry (LC-MS) analysis, and the level also correlated with the degree of dwarfism. Exogenous treatment of transgenic plants with gibberellic acid (GA3) resulted in an increased shoot length, indicating that the GA signaling pathway might normally function in transgenic plants. Thus, morphological changes in transgenic plants may result from a decrease in the endogenous levels of bioactive GAs. Finally, a possibility of molecular breeding for plant form alteration in liliaceous ornamental plants by genetically engineering the GA metabolic pathway is discussed.

Regulation of some carbohydrate metabolism-related genes, starch and soluble sugar contents, photosynthetic activities and yield attributes of two contrasting rice genotypes subjected to salt stress.

Soluble carbohydrates play a key role as osmolytes and significantly contribute in salt defence mechanism, especially in halophyte species. The objective of this study is to investigate the transcriptional expression of starch-related genes, sugar profile and physiological performances of two contrasting rice genotypes, Pokkali (salt tolerant) and IR29 (salt sensitive), in response to salt stress. Total soluble sugars, glucose and fructose levels in the flag leaf of salt-stressed Pokkali rice were enhanced relative to soluble starch accumulation in plants exposed to EC = 13.25 dS m(-1) (salt stress) for 3 days. In Pokkali, the net photosynthetic rate and starch metabolism may play a key role as energy resources under salt stress. In contrast, photosynthetic performance, indicated by photosynthetic pigment levels and chlorophyll fluorescence parameters, in salt-stressed IR29 was significantly reduced, leading to delayed starch biosynthesis. The reduction in photosynthetic ability and lack of defence mechanisms in IR29 caused growth inhibition and yield loss. Soluble starch and soluble sugar enrichment in Pokkali rice may function alternatively as osmotic adjustment in salt defence mechanism and strengthen carbon energy reserves, greater survival prospects under salt stress and enhanced productivity.

Chemical and bioactivity evaluation of the bark of Neonauclea purpurea.

Bioassay-guided fractionation of the MeOH extract from the stem bark of Neonauclea purpurea used in traditional medicine, resulted in the isolation of 2 indole alkaloids, cadambine (1) and alpha-dihydrocadambine (2), as well as a quinolic compound, 2,6-dimethoxy-1,4-benzoquinone (3). Antimalarial activity evaluation showed that compounds 2 and 3 exhibited mild in vitro antimalarial activity against Plasmodium falciparum, the chloroquine-resistant strain K1 with IC50 values of 6.6 and 11.3 microM, respectively. Compounds 1 and 2 showed no cytotoxicity to monkey (Vero) cells, but compound 3 showed weak cytotoxicity with an IC50 value of 1.19 microM.

Transcriptional regulations of the genes of starch metabolism and physiological changes in response to salt stress rice (Oryza sativa L.) seedlings.

The aim of this investigation was to compare the transcriptional expression of starch metabolism, involving genes and physiological characters, in seedlings of two contrasting salt-tolerant rice genotypes, in response to salt-stress. The soluble sugar content in rice seedlings of both salt-tolerant and salt-sensitive genotypes was enriched, relating to starch degradation, in plants subjected to 200 mM NaCl. In the salt-tolerant cultivar Pokkali, a major source of carbon may be that derived from the photosynthetic system and starch degradation. In starch degradation, only Pho and PWD genes in Pokkali were upregulated in plants subjected to salt stress. In contrast, the photosynthetic abilities of IR29 salt-susceptible cultivar dropped significantly, relating to growth reduction. The major source of carbohydrate in salt-stressed seedlings of the IR29 cultivar may be gained from starch metabolism, regulated by ADP-glucose pyrophosphorylase (AGP), starch synthase (SS), starch branching enzyme (SBE), starch debranching enzyme (ISA), glucan-water dikinase (GWD), dispropotionating enzyme (DPE), phospho glucan-water dikinase (PWD) and starch phosphorylase (Pho). Also, the major route of soluble sugar in salt-stressed Pokkali seedlings was derived from photosynthesis and starch metabolism. This was identified as novel information in the present study.