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1.
Genome Announc ; 5(1)2017 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-28057749

RESUMO

The genome of Ralstonia solanacearum CaRs_Mep, a race 4/biovar 3/phylotype I bacterium causing wilt in small cardamom and other Zingiberaceae plants, was sequenced. Analysis of the 5.7-Mb genome sequence will aid in better understanding of the genetic determinants of host range, host jump, survival, pathogenicity, and virulence of race 4 of R. solanacearum.

2.
J Biosci ; 38(2): 267-78, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23660661

RESUMO

Ribosomal gene sequences are a popular choice for identification of bacterial species and, often, for making phylogenetic interpretations. Although very popular, the sequences of 16S rDNA and 16-23S intergenic sequences often fail to differentiate closely related species of bacteria. The availability of complete genome sequences of bacteria, in the recent years, has accelerated the search for new genome targets for phylogenetic interpretations. The recently published full genome data of nine strains of R. solanacearum, which causes bacterial wilt of crop plants, has provided enormous genomic choices for phylogenetic analysis in this globally important plant pathogen. We have compared a gene candidate recN, which codes for DNA repair and recombination function, with 16S rDNA/16-23S intergenic ribosomal gene sequences for identification and intraspecific phylogenetic interpretations in R. solanacearum. recN gene sequence analysis of R. solanacearum revealed subgroups within phylotypes (or newly proposed species within plant pathogenic genus, Ralstonia), indicating its usefulness for intraspecific genotyping. The taxonomic discriminatory power of recN gene sequence was found to be superior to ribosomal DNA sequences. In all, the recN-sequence-based phylogenetic tree generated with the Bayesian model depicted 21 haplotypes against 15 and 13 haplotypes obtained with 16S rDNA and 16-23S rDNA intergenic sequences, respectively. Besides this, we have observed high percentage of polymorphic sites (S 23.04%), high rate of mutations (Eta 276) and high codon bias index (CBI 0.60), which makes the recN an ideal gene candidate for intraspecific molecular typing of this important plant pathogen.


Assuntos
Proteínas de Bactérias/genética , Enzimas de Restrição do DNA/genética , Tipagem Molecular/métodos , Doenças das Plantas/microbiologia , RNA Ribossômico 16S/genética , Ralstonia solanacearum/genética , Sequência de Bases , Teorema de Bayes , Códon , Reparo do DNA , DNA Espaçador Ribossômico/genética , Haplótipos , Filogenia , Polimorfismo Genético , RNA Bacteriano/genética , Ralstonia solanacearum/classificação , Ralstonia solanacearum/isolamento & purificação , Recombinação Genética , Análise de Sequência de DNA
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