HC-Pro of Triticum Mosaic Virus is a Viral Determinant of Wheat Curl Mite Transmission.

Triticum mosaic virus (TriMV; genus Poacevirus; family Potyviridae) is an economically important virus in the Great Plains region of the USA. TriMV is transmitted by wheat curl mite (Aceria tosichella Keifer) Type 2 genotype but not by Type 1. HC-Pro is a vector transmission determinant for several potyvirids, but the role of HC-Pro in TriMV transmission is unknown. In this study, we examined the requirement of HC-Pro cistron of TriMV for wheat curl mite (Type 2) transmission through deletion and point mutations and constructing TriMV chimeras with heterologous HC-Pros from other potyvirids. TriMV with complete deletion of HC-Pro failed to be transmitted by wheat curl mites at detectable levels. Furthermore, TriMV chimeras with heterologous HC-Pros from aphid-transmitted turnip mosaic virus and tobacco etch virus, or wheat curl mite-transmitted wheat streak mosaic virus, failed to be transmitted by wheat curl mites. These data suggest that heterologous HC-Pros did not complement TriMV for wheat curl mite transmission. A decreasing series of progressive nested in-frame deletions at the N-terminal region of HC-Pro comprising amino acids 3-125, 3-50, 3-25, 3-15, 3-8, and 3 and 4 abolished TriMV transmission by wheat curl mites. Additionally, mutation of conserved His20, Cys49, or Cys52 to Ala in HC-Pro abolished TriMV transmissibility by wheat curl mites. These data suggest that the N-terminal region of HC-Pro is crucial for TriMV transmission by wheat curl mites. Collectively, these data demonstrate that the HC-Pro cistron of TriMV is a viral determinant for wheat curl mite transmission.

Morphological and genetic variability in cosmopolitan tardigrade species-Paramacrobiotus fairbanksi Schill, Förster, Dandekar & Wolf, 2010.

Paramacrobiotus fairbanksi was described from Alaska (USA) based on integrative taxonomy and later reported from various geographical localities making it a true cosmopolitan species. The 'Everything is Everywhere' (EiE) hypothesis assumes that the geographic distribution of microscopic organisms is not limited by dispersal but by local environmental conditions, making them potentially cosmopolitan. In the present work we report four new populations of P. fairbanksi from the Northern Hemisphere which suggests that the 'EiE' hypothesis is true, at least for some tardigrade species. We also compared all known populations of P. fairbanksi at the genetic and morphological levels. The p-distances between COI haplotypes of all sequenced P. fairbanksi populations from Albania, Antarctica, Canada, Italy, Madeira, Mongolia, Spain, USA and Poland ranged from 0.002 to 0.005%. In total, twelve haplotypes (H1-H12) of COI gene fragments were identified. We also report statistically significant morphometrical differences of species even though they were cultured and bred in the same laboratory conditions. Furthermore, we also discuss differences in the potential distribution of two Paramacrobiotus species.

Morphological and genetic variability in cosmopolitan tardigrade species - Paramacrobiotus fairbanksi Schill, Förster, Dandekar & Wolf, 2010.

Paramacrobiotus fairbanksi was described from Alaska (USA) based on integrative taxonomy and later reported from various geographical locations making it a true cosmopolitan species. The 'Everything is Everywhere' (EiE) hypothesis assumes that microscopic organisms have unique features that help them to inhabit many different environments, meaning they can be considered cosmopolitan. In the present work we report four new populations of Pam. fairbanksi from the Northern Hemisphere which suggests that the 'EiE' hypothesis is true, at least for some tardigrade species. We also compared all known populations of Pam. fairbanksi at the genetic and morphological levels. The p-distances between COI haplotypes of all sequenced Pam. fairbanksi populations from Albania, Antarctica, Canada, Italy, Madeira, Mongolia, Spain, USA and Poland ranged from 0.002% to 0.005%. In total, twelve haplotypes (H1-H12) of COI gene fragments were identified. We also report statistically significant morphometrical differences of species even though they were cultured and bred in the same laboratory conditions, and propose epigenetic factor as a main cause rather than temperature, predation risk and food availability. Furthermore, we also discuss differences in the potential distribution of two Paramacrobiotus species.

Cryptic diversity within grass-associated Abacarus species complex (Acariformes: Eriophyidae), with the description of a new species, Abacarus plumiger n. sp.

Accurate estimation of species richness is often complex as genetic divergence is not always accompanied by appreciable morphological differentiation. In consequence, cryptic lineages or species evolve. Cryptic speciation is common especially in taxa characterized by small and simplified bodies, what makes their proper identification challenging. The cereal rust mite, Abacarus hystrix, was regarded for a long time as a species associated with a wide range of grass hosts, whereas wide host ranges are rather rare in eriophyoid mites. Therefore, the generalist status of A. hystrix was questioned. In this paper we demonstrate that the diversity within Abacarus species associated with grasses is more complex than it was previously thought. The 78 Abacarus mtDNA COI sequences used in this study formed 10 highly supported clades (bootstrap value 99%) and four more distinct genetic lineages were represented by unique sequences. The genetic distances between them ranged from 6.6 to 26.5%. Moreover, morphological study and genetic approach based on the combination of the Poisson Tree Processes model for species delimitation (PTP) and a Bayesian implementation of PTP (bPTP), and Neighbour Joining analyses led to delimitation of a new species within the Abacarus complex: Abacarus plumiger, specialized on smooth brome (Bromus inermis). Furthermore, our analyses demonstrated a pattern of host-associated differentiation within the complex. Overall, our study indicates that cryptic speciation occurs in the grass-associated Abacarus genus, and suggests the need for more extensive sampling using integrative methods.

Genetics of lineage diversification and the evolution of host usage in the economically important wheat curl mite, Aceria tosichella Keifer, 1969.

BACKGROUND: Understanding the mechanisms that underlie the diversification of herbivores through interactions with their hosts is important for their diversity assessment and identification of expansion events, particularly in a human-altered world where evolutionary processes can be exacerbated. We studied patterns of host usage and genetic structure in the wheat curl mite complex (WCM), Aceria tosichella, a major pest of the world's grain industry, to identify the factors behind its extensive diversification. RESULTS: We expanded on previous phylogenetic research, demonstrating deep lineage diversification within the taxon, a complex of distinctive host specialist and generalist lineages more diverse than previously assumed. Time-calibrated phylogenetic reconstruction inferred from mitochondrial DNA sequence data suggests that lineage diversification pre-dates the influence of agricultural practices, and lineages started to radiate in the mid Miocene when major radiations of C4 grasses is known to have occurred. Furthermore, we demonstrated that host specificity is not phylogenetically constrained, while host generalization appears to be a more derived trait coinciding with the expansion of the world's grasslands. Demographic history of specialist lineages have been more stable when compared to generalists, and their expansion pre-dated all generalist lineages. The lack of host-associated genetic structure of generalists indicates gene flow between mite populations from different hosts. CONCLUSIONS: Our analyses demonstrated that WCM is an unexpectedly diverse complex of genetic lineages and its differentiation is likely associated with the time of diversification and expansion of its hosts. Signatures of demographic histories and expansion of generalists are consistent with the observed proliferation of the globally most common lineages. The apparent lack of constrains on host use, coupled with a high colonization potential, hinders mite management, which may be further compromised by host range expansion. This study provides a significant contribution to the growing literature on host-association and diversification in herbivorous invertebrates.

Population growth rate of dry bulb mite, Aceria tulipae (Acariformes: Eriophyidae), on agriculturally important plants and implications for its taxonomic status.

Dry bulb mite (DBM), Aceria tulipae, is an economically important mite with a worldwide distribution and a broad host range. As a generalist, it is the most important eriophyoid mite attacking bulbous plants such as garlic, onion and tulip. To date, DBM has been recorded on host plants belonging to the families Liliaceae, Amaryllidaceae, Melanthiaceae and Asparagaceae. However, a precise understanding of DBM host range is lacking as it is largely based on casual records of mites on plants, some of which may include accidental hosts. Moreover, the possible existence of cryptic species has not been considered. In this study the hypothesis that DBM may be a complex of distinct genetic lineages or cryptic species was tested by comparing the common barcode sequence marker mtDNA COI of specimens from several populations originating from the Netherlands and Poland. The population growth rate of DBM on seven agriculturally important plant species and on various parts of the garlic plant was also experimentally assessed in the laboratory. The results did not support the first hypothesis, and indicated that DBM populations originating from Poland and the Netherlands shared essentially the same genome. In addition, they indicated that DBM reached the highest population growth rate on leek and also displayed high growth rates on garlic, chive and red onion, whereas white onion and wheat were not colonized by the mites. Answering the question of whether DBM is a single polyphagous species rather than a complex of cryptic lineages is of particular importance since the misidentification of pests may lead to ineffective control strategies. Moreover, improved knowledge of DBM host range is essential for assessing risk to crops.

Spatial and Host-Related Variation in Prevalence and Population Density of Wheat Curl Mite (Aceria tosichella) Cryptic Genotypes in Agricultural Landscapes.

The wheat curl mite (WCM), Aceria tosichella Keifer, is a major pest of cereals worldwide that also comprises a complex of at least 16 genetic lineages with divergent physiological traits, including host associations and specificity. The goal of this study was to test the extent to which host-plant species and landscape spatial variation influence WCM presence and population density across the entire area of Poland (>311,000 km2). Three important findings arose from the results of the study. (1) The majority of WCM lineages analyzed exhibited variation in patterns of prevalence and/or population density on both spatial and host-associated scales. (2) Areas of occurrence and local abundance were delineated for specific WCM lineages and it was determined that the most pestiferous lineages are much less widespread than was expected, suggesting relatively recent introductions into Poland and the potential for further spread. (3) The 16 WCM lineages under study assorted within four discrete host assemblages, within which similar host preferences and host infestation patterns were detected. Of these four groups, one consists of lineages associated with cereals. In addition to improving basic ecological knowledge of a widespread arthropod herbivore, the results of this research identify high-risk areas for the presence of the most pestiferous WCM lineages in the study area (viz. the entirety of Poland). They also provide insight into the evolution of pest species of domesticated crops and facilitate testing of fundamental hypotheses about the ecological factors that shape this pest community.

Eriophyoid mites (Acari: Prostigmata: Eriophyoidea) from Turkey: description of five new species.

Five new eriophyoid mite species (Eriophyidae) from Turkey are described and illustrated in this paper: Aceria vanensis n. sp., Aceria onosmae n. sp., Aculus lydii n. sp., Aculus gebeliae n. sp. and Aculus spectabilis n. sp.. The descriptions are based on the morphology of females collected from weedy plants, respectively: Amaranthus retroflexus L. (Amaranthaceae), Onosma isauricum Boiss. et Heldr. (Boraginaceae), Hypericum lydium Boiss. (Hypericaceae), Lotus gebelia Vent. (Fabaceae) and Stachys spectabilis Choisy ex DC. (Lamiaceae). The new species were found to be vagrant on their host plants with no visible damage symptoms observed.

Thermal Niches of Two Invasive Genotypes of the Wheat Curl Mite Aceria tosichella: Congruence between Physiological and Geographical Distribution Data.

The wheat curl mite (WCM), Aceria tosichella Keifer, is a major pest of cereals worldwide. It is also a complex of well-defined genetic lineages with divergent physiological traits, which has not been accounted for in applied contexts. The aims of the study were to model the thermal niches of the two most pestiferous WCM lineages, designated MT-1 and MT-8, and to assess the extent to which temperature determines the distribution of these lineages. WCM population dynamics were modeled based on thermal niche data from March to November on the area of Poland (>311,000 km2). The most suitable regions for population development were predicted and compared to empirical field abundance data. Congruence between modeled parameters and field data for mite presence were observed for both WCM lineages although congruence between modeled thermal suitability and mite field abundance was observed only for MT-8. Thermal niche data for MT-1 and MT-8 provide biological insights and aid monitoring and management of WCM and the plant viruses it vectors. The presented models accurately estimate distributions of WCM and can be incorporated into management strategies for both current and predicted climate scenarios.

Genetic and morphological diversity of Trisetacus species (Eriophyoidea: Phytoptidae) associated with coniferous trees in Poland: phylogeny, barcoding, host and habitat specialization.

Eriophyoid species belonging to the genus Trisetacus are economically important as pests of conifers. A narrow host specialization to conifers and some unique morphological characteristics have made these mites interesting subjects for scientific inquiry. In this study, we assessed morphological and genetic variation of seven Trisetacus species originating from six coniferous hosts in Poland by morphometric analysis and molecular sequencing of the mitochondrial cytochrome oxidase subunit I gene and the nuclear D2 region of 28S rDNA. The results confirmed the monophyly of the genus Trisetacus as well as the monophyly of five of the seven species studied. Both DNA sequences were effective in discriminating between six of the seven species tested. Host-dependent genetic and morphological variation in T. silvestris and T. relocatus, and habitat-dependent genetic and morphological variation in T. juniperinus were detected, suggesting the existence of races or even distinct species within these Trisetacus taxa. This is the first molecular phylogenetic analysis of the Trisetacus species. The findings presented here will stimulate further investigations on the evolutionary relationships of Trisetacus as well as the entire Phytoptidae family.

Phylogenetic analyses reveal extensive cryptic speciation and host specialization in an economically important mite taxon.

The wheat curl mite (WCM) is a major pest in cereal crops around the world and the vector of at least four known pathogens capable of reducing yields in crops such as wheat, corn, barley, oats, millet and rye. Current taxonomy recognizes WCM as a single species, Aceriatosichella; however, recent genetic, physiological and ecological studies have shown that WCM is likely to be a species complex. In this study we assessed genetic variation and phylogenetic relationships among WCM from four continents and a wide range of host plants using DNA sequence data from one mitochondrial gene, one nuclear gene and a single nuclear intergenic spacer region. Phylogenetic analyses revealed 11 unique mite lineages associated with specific plant hosts including wheat and barley. Host associations were consistent across continents, often with a single haplotype dominating a host plant regardless of geographic origin. The genetic and ecological differences identified in this study support the notion that WCM is a species complex in need of major taxonomic revision. These findings have implications for control of WCM globally, particularly within the context of identifying plants that form 'green bridge' refuges, assessing disease transmission risk, and identifying resistance in cereal genotypes to WCM and associated pathogens.

Wheat curl mite, Aceria tosichella, and transmitted viruses: an expanding pest complex affecting cereal crops.

The wheat curl mite (WCM), Aceria tosichella, and the plant viruses it transmits represent an invasive mite-virus complex that has affected cereal crops worldwide. The main damage caused by WCM comes from its ability to transmit and spread multiple damaging viruses to cereal crops, with Wheat streak mosaic virus (WSMV) and Wheat mosaic virus (WMoV) being the most important. Although WCM and transmitted viruses have been of concern to cereal growers and researchers for at least six decades, they continue to represent a challenge. In older affected areas, for example in North America, this mite-virus complex still has significant economic impact. In Australia and South America, where this problem has only emerged in the last decade, it represents a new threat to winter cereal production. The difficulties encountered in making progress towards managing WCM and its transmitted viruses stem from the complexity of the pathosystem. The most effective methods for minimizing losses from WCM transmitted viruses in cereal crops have previously focused on cultural and plant resistance methods. This paper brings together information on biological and ecological aspects of WCM, including its taxonomic status, occurrence, host plant range, damage symptoms and economic impact. Information about the main viruses transmitted by WCM is also included and the epidemiological relationships involved in this vectored complex of viruses are also addressed. Management strategies that have been directed at this mite-virus complex are presented, including plant resistance, its history, difficulties and advances. Current research perspectives to address this invasive mite-virus complex and minimize cereal crop losses worldwide are also discussed.