RESUMO
Angelica sylvestris L. var. stenoptera Avé-Lall ex Boiss. (ASS) is an endangered endemic plant to Turkey. The objective of the study was to determine antioxidant activities, total phenolics, and phytochemical properties of methanolic extracts (MEs) and essential oil (EO) from ASS for the first time with the methods of 2,2-diphenyl-1-picrylhydrazyl (DPPHâ ) radical scavenging activities, 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid (ABTSâ + ), ferric reducing/antioxidant power (FRAP), the Folin-Ciocalteu, liquid chromatography-tandem mass spectrometry (LC-MS/MS), and gas chromatography-mass spectrometry (GC-MS), respectively. The leaf extract of ASS was found to be the richest in phenolic content (543.91±6.33 GAE, µg/mL) and showed the highest DPPHâ and FRAP activities (IC50 : 0.1140±0.0011â mg/mL, 675.62±15.01â µM TEAC). EO of ASS root showed DPPHâ and FRAP activities (IC50 : 1.3248±0.0572â mg/mL, 346.67±12.75â µM TEAC). 19 phenolics were detected in MEs of different parts of ASS by LC/MS/MS. In the chemical composition of ASS root EO by GC/MS, globulol (70.70 %) was found to be the major compound. Our results indicate that ASS can be used a source of phytochemicals and antioxidants for conservation and sustainability of endangered plants.
Assuntos
Angelica , Óleos Voláteis , Plantas Medicinais , Antioxidantes/química , Cromatografia Líquida , Óleos Voláteis/farmacologia , Óleos Voláteis/química , Fenóis/farmacologia , Fenóis/análise , Compostos Fitoquímicos/química , Extratos Vegetais/farmacologia , Extratos Vegetais/química , Plantas Medicinais/química , Ácidos Sulfônicos , Espectrometria de Massas em Tandem , TurquiaRESUMO
This work reports the chemical composition, antimicrobial and antioxidant activities of essential oils from the aerial parts of Gentiana gelida BIEB. for the first time in literature. The oils from the aerial parts (flower, leaf and stem) were obtained by Clevenger-type apparatus and characterized by GC-FID and GC-MS. While tricosane (21.67%) was the main component of flower oil, hexadecanoic acid was the most abundant component of leaf and stem oils in ratios 26.46% and 31.89%, respectively. Additionally, all essential oils of G. gelida were investigated for their antimicrobial activity against eight Gram negative bacteria, four Gram positive bacteria and five fungi, using agar dilution method and antioxidant activities by using 2,2-diphenyl-1-picrylhydrazyl radical scavenging and Folin-Ciocalteu assays. The flower oil of G. gelida showed stronger antimicrobial and antioxidant activities than those of stem and leaf. The amount of total phenolic content and scavenging activity of the flower oil were found 525.35±8.24 mg GAE/L and 49.30±1.25%, respectively.