Welfare of broiler chickens reared in two different industrial house types during the winter season in Southern Brazil.

1. The following trial compared broiler chicken welfare in closed-sided (CS) versus open-sided (OS) industrial house types during the winter season in the South of Brazil.2. Ten flocks in each house type were evaluated as follows: a) bird health: contact dermatitis on the breast and abdominal areas (CDE), bird soiling (BSO), footpad dermatitis (FPD), hock burn (HBU), lameness (LAM), fractures (FRA), bruising (BRU), scratches (SCR), dead on arrival (DOA), and diseases (DIS); b) house environmental measurements: relative humidity (RHU), temperature (TEM), air velocity (AVE), illuminance (ILL), ammonia concentration (NH3), and carbon dioxide concentration (CO2), and c) bird behaviour and affective states: bird behaviour (BBE), touch test (TTE), and qualitative behaviour assessment (QBA).3. Statistical analyses were based on regression models for CDE, BSO, FPD, HBU, LAM and generalised linear models for DOA, FRA, BRU, SCR, and DIS. The Mann-Whitney test was used for RHU, TEM, AVE, ILL, NH3, CO2, and the t-test for TTE and LMO, with a specific regression model for BBE data and Principal Component Analysis for QBA.4. According to odds ratio for worse scores for CS relative to OS, birds were less likely to have severe scores for CDE (P = 0.040 and P = 0.007), BSO (P = 0.031, P = 0.016, and P = 0.038), and HBU (P = 0.017), and had higher median values for AVE (2.3, 0.0-7.8 m s-1 vs. 0.0, 0.0-4.3 m s-1), lower NH3 concentration (9.0, 0.0-64.0 ppm vs. 12.0, 0.0-60.0 ppm) and TTE scores (98, 96-100 vs. 67, 25-100). Worse results were observed in CS houses for higher stocking density (13.8 ± 0.2 birds/m2 vs. 12.0 ± 0.2 birds/m2), RHU (74.5, 50.7-99.9% vs 72.3, 47.4-99.9%), and TEM (23.9, 14.6-29.2°C vs. 21.7, 12.9-30.1°C), lower ILL (16.0, 1.0-60.0 lx vs. 161.0, 8.0-2380.0 lx), less drinking (P = 0.007), more inactive behaviour (P < 0.001) and lower positive emotions, according to QBA (P = 0.028).5. In the studied region and season, CS houses seemed to offer fewer welfare problems in terms of the health indicators; however, OS houses showed fewer behavioural restrictions and higher positive emotional states.

Spatial distance and reactivity traits alter the positive perception of brushing by ewes.

Gentle handling seems to elicit positive states in sheep. The study investigated whether spatial distance alters sheep responses to brushing and whether spatial distance is influenced by reactivity. Twenty Romane ewes were assessed in three sessions: in Sessions 1 and 3, one grid separated the test animal from pen mates, with no distance between them, and in Session 2 two grids separated the test animal from pen mates by a distance of about 1.7 m. Ewes had been genetically selected for low (R-) or high (R+) behavioural reactivity to social isolation. Body postures, head orientation, ear postures, closed and half-closed eyes, tail wagging and feeding behaviour, in addition to heart rate (HR) and HR variability, as the root mean square of successive differences (RMSSD), standard deviation of all normal-to-normal (NN) intervals (SDNN), RMSSD/SDNN ratio and ratio between low-frequency (LF) and high-frequency (HF) powers (LF/HF) were assessed. Data were analysed using generalized linear models and linear mixed models. Session, genetic line and phase (pre-, brushing and post-brushing) were considered fixed effects. Increased distance in Session 2 might not have influenced ewes' responses. Fewer changes in ear postures were noted in Session 3 than 1 (P<0.01), suggesting that ewes were more relaxed in Session 3. The RMSSD/SDNN ratio was higher mainly during brushing in Sessions 1 and 3 (P<0.05), indicating that ewes were more relaxed during brushing, and at no distance between pen mates. However, spatial distance influenced R- and R+ ewes' responses; R+ ewes performed more asymmetric ear postures in Session 2 than 1 and 3 (P<0.01), and in Session 3 than 1 (P<0.01), indicating that spatial distance had a negative effect on R+ ewes. Low reactive ewes spent less time on horizontal ear postures in Session 2 than 1 and 3 (P<0.01), and R+ ewes spent more time on horizontal postures in Session 1 than 3 (P<0.01). Curiously, R- ewes spent more time eating and ruminating in Session 3 than 1 (P<0.01), and in Session 2 than 1 and 3 (P<0.01), whereas R+ ewes ate and ruminated more in Session 1 than 3 (P<0.05). Higher HR was found among R- ewes in Session 2 than 1 and 3, and in Session 3 than 1 (P<0.01). High reactive ewes showed higher HR in Session 1 than 3 (P<0.01). The findings suggest that the social context might influence sheep responses to gentle handling, and the effects depend on their reactivity traits.

Lean body mass: reference values for Italian population between 18 to 88 years old.

OBJECTIVE: Body's lean mass compartment is a strong predictor of morbidity and mortality risk in various clinical conditions. This paper proposes a simple and easily applied reference table for lean body mass (LBM) and lean body mass index (LBMI) for the Italian population. PATIENTS AND METHODS: Retrospective analysis of a database containing anthropometric and DXA body composition measurements obtained from a cross-sectional study conducted between 2002 and 2009 with Italian individuals. Parametric and nonparametric tests were performed using R 3.1.1 and SPSS 22.0 software packages. RESULTS: The 3712 study participants, 37.3% men and 62.7% women, aging from 18 to 88 years. Individuals with normal weight, overweight and obesity were evenly distributed in the sample. LBM and LBMI measures were significantly higher in males. In both genders, there was a significant and progressive decline in these measures associated with aging. Significant differences in LBMI between genders were found in all age groups except for individuals over 75 years. CONCLUSIONS: Based on the participants LBM profile, a reference table for LBM values was proposed. This reference will be useful to detect changes in the LBM compartment of individuals from the South Central Region of Italy, supporting health professionals during the process of diagnosing sarcopenia.

Identification of the SP22 sperm protein in Santa Inês and Dorper rams.

The sperm membrane protein referred to as SP22 has been identified in different species and, at least in rats, is highly correlated with fertility. The goals of this study were to identify and to quantify the SP22 protein on spermatozoa from adult rams (Dorper and Santa Inês breeds), and to correlate its levels to morphological and kinematics parameters. SP22 on ram sperm was effectively quantified by both enzyme-linked immunosorbent assay (ELISA) and fluorescein isothiocyanate immunostaining analysis and the two methods were significantly correlated (R(2) = 0.70). Clustering analysis of motility parameters obtained by computer-assisted semen analysis system was used to establish that three distinct kinematic subpopulations with different vigour and progressiveness coexistent within ejaculate. While there were significant differences in the distribution of the three subpopulations in the rams, there was no significant correlation between the proportion of each subpopulation in the rams and the SP22 levels. Quantification of SP22 immunostaining intensity was not correlated with any of the sperm parameters. However, SP22 levels obtained by ELISA were negatively correlated with morphological abnormalities and positively correlated with membrane integrity (three variable R(2) = 0.47). Future breeding studies are now needed to validate that this protein is a biomarker of fertility in this species.

Impact of 24-h cooling prior to freezing on the survival of domestic cat (Felis catus) epididymal sperm.

The aim of this study was to investigate the impact of a 24-h cooling period prior to freezing on domestic cat epididymal sperm viability. Fifteen tomcats were submitted to routine orchiectomy and sperm samples were retrieved from both epididymides in a Tris-glucose-20% egg yolk extender. For each tomcat, the diluted sperm was split into two equal volumes and cooled to 5 degrees C at a rate of 0.5 degrees C/min; one sample for 60 min (control) and the other for 24 h (cooled). After the cooling period, samples from both groups were frozen using an identical freezing protocol. Sperm samples were evaluated in three different periods: immediately after harvesting, after cooling at 5 degrees C for 24 h (cooled group) and after freezing-thawing of control and cooled groups. Evaluations consisted of sperm motility and progressive status, sperm morphology and plasma membrane integrity (PMI) using two fluorescent probes. After cooling for 24 h, a decrease (p < 0.05) in sperm motility, progressive status and PMI was observed when compared to sperm samples immediately after collection. Comparing the results obtained after thawing, no difference (p < 0.05) was found regarding sperm motility, progressive status, PMI and sperm morphology between control and cooled groups. The results from the present study show that cooling cat epididymal spermatozoa at 5 degrees C for 24 h prior to freezing does not lead to major damage of spermatozoa impairing the freeze-thaw process.