Efficacy of Albizia malacophylla (A.Rich.) Walp. (Leguminosae) methanol (80%) leaf extract and solvent fractions against Plasmodium berghei-induced malaria in mice model.

ETHNOPHARMACOLOGICAL RELEVANCE: Novel drugs are needed to address the issue of malarial infection resistance; natural items can be a different source of these medications. Albizia malacophylla (A. Rich.) Walp. (Leguminosae) is listed as one of the antimalarial medicinal plants in Ethiopian folk medicine. However, there are no reports regarding the biological activity or phytochemistry of the plant. AIM OF THE STUDY: Thus, this study aimed to evaluate the A. malacophylla crude extract and solvent fractions' in vivo antimalarial activity utilizing 4-day suppressive, preventative, and curative tests in mice infected with P. berghei. MATERIALS AND METHODS: The parasite Plasmodium berghei, which causes rodent malaria, was used to infect healthy male Swiss Albino mice, weighing 23-28 g and aged 6-8 weeks. Solvent fractions such as methanol, water, and chloroform were given in addition to an 80% methanolic extract at 100, 200, and 400 mg/kg doses. A Conventional test such as parasitemia, survival time, body weight, temperature, and packed cell capacity were employed to ascertain factors such as the suppressive, curative, and preventive tests. RESULTS: Every test substance dramatically reduced the number of parasites in every experiment. crude extract (with the highest percentage suppression of 67.78%) performs better antimalarial effect than the methanol fraction, which is the most efficient solvent fraction with a percentage suppression of 55.74%. With a suppression value of 64.83% parasitemia level, the therapeutic effects of 80% methanolic crude extract were greater than its curative and preventative effects in a four-day suppressive test. The survival period (17 days) was longer with the hydroalcoholic crude extract dose of 400 mg/kg than with other doses of the materials under investigation. CONCLUSIONS: The results of this investigation validate the antimalarial characteristics of A. malacophylla leaf extract. The crude extract prevented weight loss, a decline in temperature, and a reduction in PCV. The results demonstrate that the plant has a promising antimalarial effect against P. berghei, hence supporting the traditional use of the plant. Therefore, it could serve as a foundation for the development of new antimalarial drugs.

Antinociceptive and antiinflammatory activities of crude leave extract and solvent fractions of Commelina latifolia Hochst. ex C.B.Clarke (Commelinaceae) leaves in murine model.

Ethnopharmacological relevance: In the past, Ethiopian traditional medicine employed the leaves of the native Commelina latifolia Hochst. ex C.B. Clarke plant to treat wounds, pain, and malaria. Aim of the study: The crude extract and solvent fractions of C. latifolia Hochst. ex C.B. Clarke leaves were examined in the present investigation to determine their ability to have an antiinflammatory effect and provide an antinociceptive effect in animal models. Materials and methods: The leaves of C. latifolia were extracted with 80% methanol, and the CL crude extract was further fractionated with chloroform, pure methanol, and distilled water. The carrageenan-induced paw edema model was used to test the extracts' ability to reduce inflammation. The hotplate model and the acetic acid-induced writhing test on rodents were used to test the extracts' potential antinociceptive effect to reduce pain. Results: Inflammation was decreased by 64.59% with CL crude extract (400 mg/kg); 56.34% (400 mg/kg) of methanol fraction, 64.59% of aqueous fraction (400 mg/kg), and 38.27% of chloroform fraction in the carrageenan-induced inflammatory model. All extracts demonstrated a considerable lengthening of the nociception reaction time in the hot plate test, with a maximum antinociceptive effect of 78.98% (crude extract) and 71.65% (solvent fractions). At a dosage of 400 mg/kg, the natural C. latifolia crude extract and aqueous fraction demonstrated considerable antinociceptive effects against acetylsalicylic acid (ASA) during the writhing test (48.83% and 45.37than%, respectively). The current findings support Ethiopia's traditional user's assertions that the herb can alleviate inflammation and pain.

Antimalarial activity of the 80%methanol extract and solvent fractions of Cucumis ficifolius A. rich roots against Plasmodium berghei in mice.

Ethnopharmacological relevance: Malaria is still a known health threat, especially in parts of sub-Saharan Africa. It is one of the frequently mentioned issues with hospital admission and outpatient care in Ethiopia. Cucumis ficifolius A. Rich roots are historically used in Ethiopia to treat meningitis, inflammation, and malaria. However, the antimalarial activity of this plant has not been scientifically studied so far. Aim of the study: This study aimed to determine the in vivo antimalarial activity of 80% methanol extract and solvent fractions of the roots of Cucumis ficifolius against Plasmodium berghei infection in mice. Methods: The in vivo antimalarial activity of the 80% methanol extract and solvent fractions of Cucumis ficifolius A. Rich was evaluated by standard chemo suppressive, curative and repository tests using Plasmodium berghei (ANKA strain) in Swiss albino mice at doses of 100, 200 and 400 mg/kg/day. The level of parasitemia, survival time, variation in weight, rectal temperature, and packed cell volume of mice were determined to establish the activity of the extracts. Result: The 80% methanol extract of Cucumis ficifolius A. Rich roots had a promising suppression of parasitemia at 400 mg/kg with a chemosuppression value of 65.21 ± 1.20%. Among the solvent fractions, the chloroform fraction showed the highest antimalarial activity in the four-day suppressive test with a chemosuppression value of 55.9 ± 0.28%, followed by the n-butanol (42.9 ± 0.24%), and aqueous (40.57 ± 0.52%) fractions at a dose of 400 mg/kg. The highest survival times were observed with crude extract (15.4 ± 0.24 days) at 400 mg/kg, and chloroform fraction (13.4 + 0.24 days), though all extracts increased survival time. Conclusion: The findings of the present study collectively indicate the root extract of Cucumis ficifolius has a promising antiplasmodial activity which substantiates the traditional claim of the plant.

Antimalarial activity of the hydroalcoholic crude extract and solvent fractions of Commelina latifolia Hochst. ex C.B.Clarke (Commelinaceae) leaves against Plasmodium berghei in mice.

Ethnopharmacological relevance: In Ethiopia, the indigenous medicinal plant Commelina latifolia Hochst. ex C.B. Clarke leaves are used to treat malaria and wounds. Aim of the study: In this work, the antiplasmodial activity of Commelina latifolia crude leaf extract and solvent fractions against Plasmodium berghei-infected mice was investigated. Materials and methods: 80% methanol was used to extract the leaves of C. latifolia, and the crude extract was fractionated using chloroform, pure methanol, and distilled water. All test compounds were undergone an acute oral toxicity test before being put through Peter's 4-day suppressive test to see if they have antiplasmodial activity. The hydroalcoholic crude extract and chloroform fraction were additionally assessed for antimalarial activity using curative and prophylactic tests in P. berghei-infected laboratory mice. Results: All of the tested crude extracts were safe at a dose of 2000 mg/kg. At 400 mg/kg dose both the 80% methanol extract and chloroform fraction exhibited antimalarial activity with parasitemia suppression values of 86.31%, and 76.56% in the four-day suppressive test, 81.97% and 72.05% in Rane's test, and 69.05% and 62.88% in the prophylactic test, respectively. Conclusion: Collectively, the oral dose of Commelina latifolia is safe, and reveals promising antimalarial activity. The findings backed up the utilization of the plant in traditional medicine to treat malaria.

Suppressive, Curative, and Prophylactic Effects of Maesa lanceolata Forssk. against Rodent Malaria Parasite Plasmodium berghei.

The artemisinin partial resistance is believed to be spread to artemisinin-based combination therapy partner drugs. As a result, new antiplasmodial compounds are required to treat resistant malaria infections. In the invention of antimalarial substances, claimed medical plants are precious resources. So, the current study was designed to assess the antiplasmodial effects of Maesa lanceolata in mice. In this study, preliminary phytoconstituent and in vivo acute oral toxicity tests were done. Early infection, established infection, and residual infection tests were employed to determine the antimalarial effects of the test drugs. Three doses (200, 400, and 600 mg/kg) of the extracts were provided orally to the test mice. Analysis of variance (one-way) followed by post hoc Tukey's test was used to analyze the difference between and within groups. Terpenoids, tannins, saponins, flavonoids, and alkaloids were detected in the phytochemical constituent analysis. Both 80% methanolic crude extract and solvent fractions had no toxic result at the 2000 mg/kg dose. All test drug doses suppressed parasite levels in a significant manner at all tests. The activity of chloroform fraction (maximum percentage suppression, 81.28%) overwhelms the crude extract activity. The curative effects of 80% methanolic crude extract, with a maximum of 80.22% parasitemia suppression, were greater than its suppressive and prophylactic effects. The 400 mg/kg dose of chloroform fraction resulted in a maximum survival period (18 days) than other doses of tested materials. The results of this investigation provide support for the activity of M. lanceolata leaf extract against malaria.

Self-medication practice with modern and herbal medicines and associated factors among pregnant women attending antenatal care at Mizan-Tepi University Teaching Hospital, Southwest Ethiopia.

Introduction: Practicing self-medication using conventional and/or herbal drugs during pregnancy could contribute/result in illness and death for the mother and embryo. The focus of the current study was to investigate the level of practice and factors affecting self-medication with conventional and herbal drugs among pregnant women who were on follow-up at the antenatal care (ANC) clinic of Mizan-Tepi University teaching hospital (MTUTH), Southwest Ethiopia. Methodology: A cross-sectional study was conducted from January 1st to February 30th, 2022, among 264 pregnant mothers who were on follow-up at antenatal care of MTUTH. A lottery method was used to pick study subjects who fulfilled the inclusion criteria. An interviewer-administered structured questionnaire was used to collect data which was entered and analyzed using SPSS version 24 software. Bivariate followed by multivariate logistic regression was employed to point out factors affecting self-medication practice with conventional and herbal drugs. P-value <0.05 in multivariate analysis was used as a cut-off point to decide statistical significance. Results: It was revealed that 44.3 percent and 49.2 percent of pregnant women self-medicate with conventional and herbal medications, respectively. Women with no history of self-medication were 6.69 folds less likely to practice self-medication using conventional medicine than those with prior experience (AOR: 6.69, 95% CI, (3.847-11.659). Having no health insurance increased the odds of self-medication using conventional medicine by about 46% among pregnant women (AOR: 0.687, 95% CI, (0.373-1.264). Pregnant mothers who joined college/university education were more likely to practice self-medication than mothers without formal education (AOR: 0.656, 95% CI, (0.304-1.414). Gravidity, education level, and history of herbal medicine use were factors that influenced pregnant mothers' use of herbal medicines. Conclusion: According to the present investigation, self-medication by expectant mothers is very common; thus, education and guidance of pregnant women and their partners should be considered during their ANC follow-up to minimize self-medication-associated potential threats.

A systematic review on traditional medicinal Animal's parts and products used for the treatment of respiratory tract disorders in Ethiopia.

BACKGROUND: Respiratory diseases affect more than one billion people worldwide. Although medicinal animals have huge importance in primary health care, limited effort applied so far been in Ethiopia to properly identify, document, and conserve these animals and the related knowledge. This systematic review was aimed to identify the medicinal animals and document reports on the use of medicinal animals for the management of respiratory disorders. METHODS: Both published and unpublished articles on the ethnozoological use of medicinal animals were searched using electronic database (Science Direct, Pub Med, Google Scholar, Scopus, hinari, and research gate) and institutional repositories. Different keywords were used separately and in combination using Boolean operators "OR" or "AND" as well as medical subject heading [MeSH] terms. Studies that did not address respiratory disorders were excluded. Data were extracted using Microsoft excel spread sheet. RESULTS: Among 211 articles obtained from database searches, nine articles were eligible and included in the review. Thirty two medicinal animals were reported for treatment of respiratory disorders. Mammals were the most commonly (43.8%) used medicinal animals. Meat was reported as the most commonly (22.6%) used medicinal remedy. CONCLUSIONS: Most of the medicinal remedies were obtained from mammals and administered orally. As the majority of the medicinal animals were obtained from wild sources, due attention should be given to conserve them. Besides, this traditional medicinal knowledge should be integrated to modern medicine to investigate the acute and chronic toxicity profile and efficacy of these remedies to identify potential lead compounds to modern drugs.

Identification of lobetyolin as a major antimalarial constituent of the roots of Lobelia giberroa Hemsl.

Lobelia giberroa Hemsl. is an endogenous Ethiopian medicinal plant with a long history of use in the treatment of malaria, bacterial and fungal diseases, and cancer. Here, we present the in vivo bioassay-guided fractionation of the 80% methanol extract of L. giberroa roots, which led to the isolation of lobetyolin. L. giberroa roots were extracted with 80% methanol, and the dried 80% methanol extract was fractionated with hexane, ethyl acetate, methanol, and water. Acute oral toxicity study was conducted according to the Organisation for Economic Co-operation and Development Guideline 425 by using female Swiss albino mice. Antimalarial activity was assessed in Plasmodium berghei-infected Swiss albino mice. Through in vivo bioassay-guided fractionation processes lobetyolin, a C14-polyacetylene glucoside, was isolated from the methanol fraction by silica gel column chromatography as the main active ingredient from the plant. The chemical structure of lobetyolin was elucidated by interpretation of spectroscopic data (1HNMR, 13CNMR, IR. MS) including two dimensional NMR. The plant extract was considered safe for administration up to 2000 mg/kg. In the four-day suppressive test, the 80% methanol extract (400 mg/kg), methanol fraction (400 mg/kg), and lobetyolin (100 mg/kg) exhibited antimalarial activity, with chemosuppression values of 73.05, 64.37, and 68.21%, respectively. Compared to the negative control, which had a mean survival time of 7 days, the lobetyolin (100 mg/kg) and methanol fraction (400 mg/kg) treated groups had mean survival times of 18 and 19 days, respectively. The current study supports the traditional use of the plant for the treatment of malaria. The structural differences between lobetyolin and existing antimalarials, as well as its previously unknown antimalarial activity, make it of interest as an early lead compound for further chemical optimization.