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1.
Global Surg Educ ; 2(1): 27, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-38013871

RESUMO

Purpose: With the COVID-19 pandemic, in-person fellowship interviews were curtailed, leading candidates to seek information from other resources. Our main purposes were (1) to determine what information recent participants in the match needed to evaluate programs and (2) to assess which of these were available online. Methods: A focus group of ten recent graduates/applicants identified information that was important in choosing a fellowship program. In August 2020 and December 2021, websites belonging to the American Pediatric Surgical Association (APSA) and individual programs were assessed. Results: Recent applicants identified 55 pieces of information considered important to their decision making. Of 57 pediatric surgery fellowships, 98% were listed on APSA's website. Program descriptions on APSA's website listed on average 60% of program information desired by applicants. All listed fellowship director, accreditation status, faculty list, and current fellow(s). Other descriptors frequently noted were alumni (95%), graduate's board performance (83%), ECMO exposure (77%), and curriculum (70%). Information desired but less frequently available were fellow case logs (63%), trauma center designation (53%), burn center designation (40%), research opportunities (30%), candidate interview assistance (25%), and supplemental fellowships (12%). There were 7% of program descriptions that were not updated for at least a year. Conclusions: APSA and individual program websites were complimentary. Websites often lacked data that applicants sought to inform their rank list. To best adapt to the evolving virtual interview paradigm, we suggest reporting key information on a central APSA website with more nuanced information available via links to program specific websites. Supplementary Information: The online version contains supplementary material available at 10.1007/s44186-023-00104-w.

2.
J Robot Surg ; 17(5): 2427-2433, 2023 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-37452975

RESUMO

Robotic-assisted surgery (RAS) has a variety of theoretical advantages, including tremor filtration, optimal visualization, and improvement of surgeon ergonomics. Though it has achieved wide application in pediatric urology, the majority of pediatric general surgeons do not employ RAS. This study reports our institution's experience with RAS on a pediatric general surgery team. Following IRB approval, a retrospective review of all pediatric patients at our academic children's hospital who underwent RAS between 2017 and 2022 for pediatric general surgical conditions was performed. Patient demographics, operation performed, operating time, complications, and recovery were evaluated. A total of 159 children underwent RAS, increasing from 10 patients in 2017 to 59 patients in 2022. The median age and weight were 15.3 years and 76.4 kg, and 121 (76.1%) were female. The application of RAS was successful in all cases. There were no intraoperative complications and no conversions to an open approach. Eleven patients (6.9%) had unplanned presentations to the emergency department within 30 days. Five of these patients (3.1%) required admission to the hospital. This study demonstrates that the application of RAS in an academic pediatric general surgery practice is feasible and safe. The application of RAS to pediatric general surgery should continue to increase as operative teams increase their experience and comfort.Level of evidence Level IV.


Assuntos
Laparoscopia , Procedimentos Cirúrgicos Robóticos , Robótica , Cirurgiões , Humanos , Criança , Feminino , Masculino , Procedimentos Cirúrgicos Robóticos/métodos , Estudos Retrospectivos , Complicações Intraoperatórias
3.
Pediatr Surg Int ; 39(1): 155, 2023 Mar 22.
Artigo em Inglês | MEDLINE | ID: mdl-36944730

RESUMO

PURPOSE: Thoracoscopic and open approaches for the management of congenital lung malformations (CLM) has been debated. The aim of this study is to compare 30-day outcomes for non-emergent lobectomies in children. METHODS: The National Surgical Quality Improvement Program-Pediatric database was queried for patients undergoing CLM resection from 2013 to 2020. Outcomes were compared by operative technique in an intention-to-treat model and then propensity matched. RESULTS: 2157 patients met inclusion criteria and underwent non-emergent pulmonary lobectomy for CLM. The intended operative approach was thoracoscopic in 57.7% of patients. Patients in the open group compared to the thoracoscopic were more likely to be born premature, have chronic lung disease, require preoperative oxygen support, and be ventilator dependent. After propensity matching, there was no statistically significant difference in 30-day mortality, unplanned readmission, and other complications between the thoracoscopic and open groups. Thoracoscopic approach was associated with a shorter length of stay. The proportion of cases approached via thoracoscopy increased over time from 48.8% in 2013 to 69.9% in 2020. CONCLUSIONS: This large multicenter retrospective matched analysis demonstrates thoracoscopic lobectomy in children has similar favorable 30-day outcomes and shorter length of stay for the non-emergent management of CLM, compared to open thoracotomy. LEVEL OF EVIDENCE: Level III.


Assuntos
Pneumopatias , Neoplasias Pulmonares , Anormalidades do Sistema Respiratório , Humanos , Criança , Estudos Retrospectivos , Pneumonectomia/métodos , Resultado do Tratamento , Pneumopatias/cirurgia , Pneumopatias/congênito , Pulmão/cirurgia , Pulmão/anormalidades , Toracotomia/métodos , Anormalidades do Sistema Respiratório/cirurgia , Tempo de Internação , Neoplasias Pulmonares/cirurgia , Cirurgia Torácica Vídeoassistida/métodos
4.
J Pediatr Surg ; 58(6): 1133-1138, 2023 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-36914464

RESUMO

PURPOSE: This study describes the job market from the perspective of recent pediatric surgery graduates. METHODS: An anonymous survey was circulated to the 137 pediatric surgeons who graduated from fellowships 2019-2021. RESULTS: The survey response rate was 49%. The majority of respondents were women (52%), Caucasian (72%), and had a median student debt burden of $225,000. Considering job opportunities, respondents strongly emphasized camaraderie (93%), mentorship (93%), case mix (85%), geography (67%), faculty reputation (62%), spousal employment (57%), compensation (51%), and call frequency (45%). 30% were satisfied with the employment opportunities available, and 21% felt strongly prepared to negotiate for their first job. All respondents were able to secure a job. Most jobs were university-based (70%) or hospital employed (18%) positions where surgeons covered median of two hospitals. 49% wanted protected research time, and 12% of respondents were able to secure substantial, protected research time. The median compensation for university-based jobs was $12,583 below the median AAMC benchmark for assistant professors for the corresponding year of graduation. CONCLUSION: These data highlight the ongoing need for assessment of the pediatric surgery workforce and for professional societies and training programs to further assist graduating fellows in preparing to negotiate their first job. TYPE OF STUDY: Survey LEVEL OF EVIDENCE: Level V.


Assuntos
Internato e Residência , Especialidades Cirúrgicas , Criança , Humanos , Masculino , Feminino , Emprego , Bolsas de Estudo , Inquéritos e Questionários , Hospitais
5.
J Surg Res ; 283: 690-698, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-36459862

RESUMO

INTRODUCTION: Among premature infants, the incidence of inguinal hernias is reported to be as high as 30%. Despite being one of the most commonly performed procedures, the optimal setting of inguinal hernia repair (IHR) that is inpatient versus outpatient remains debatable. We sought to compare the 30-day outcomes of each approach by querying the National Surgical Quality Improvement Program-Pediatric database. MATERIALS AND METHODS: A retrospective cohort study comparing inpatient versus outpatient IHR using the National Surgical Quality Improvement Program-Pediatric database from 2013 to 2019 was performed. Demographic and clinical data were initially compared using univariate analysis. Continuous variables are presented as median and interquartile range and categorical variables are presented as n (%). Subsequently, cohorts were propensity matched using clinically and statistically significant patient characteristics. RESULTS: 928 patients underwent IHR, 634 (68.3%) while inpatient, 294 (31.7%) following hospital discharge. Inpatient IHR was associated with lower age at the time of surgery (120 versus 147 d; P < 0.0001), younger gestational age (27 versus 33 wk; P < 0.0001), decreased probability of repair in elective setting (87.2% versus 97.3%; P < 0.0001), and increased preoperative supplemental oxygen need (42% versus 4.4%; P < 0.0001). Comparison of propensity matched cohorts revealed that inpatient IHR was associated with increased procedure time (82 versus 51 min; P < 0.0001) and anesthetic duration (146 versus 102 min; P < 0.0001), wound infection rates (3.8% versus 0%; P = 0.007), blood transfusions (4.2% versus 0.5%; P = 0.036), unplanned intubations (2.8% versus 0%; P = 0.03), ventilator days (0 versus 0; range [0,30 versus 0,2]; P = 0.002), reoperation rate (5.6% versus 0%; P < 0.001), postoperative hospital length of stay (4 versus 1 d; P < 0.0001), and unplanned readmissions (8.9% versus 0.9%; P = 0.002). CONCLUSIONS: Inpatient IHR in premature neonates were associated with different postoperative outcomes than outpatient IHR. At least in the elective setting among premature infants, outpatient IHR can be considered safe in select patients while we await higher quality prospective data.


Assuntos
Hérnia Inguinal , Recém-Nascido , Humanos , Criança , Hérnia Inguinal/cirurgia , Estudos Retrospectivos , Estudos Prospectivos , Recém-Nascido Prematuro , Complicações Pós-Operatórias/etiologia , Herniorrafia/efeitos adversos
6.
Am Surg ; 88(10): 2531-2537, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35619551

RESUMO

BACKGROUND: Selection of pediatric surgical trainees is a multifaceted process heavily influenced by in-person interviews to determine personality makeup and institutional compatibility. We present our experience in utilizing a validated personality inventory in the selection of our candidates. METHODS: All applicants selected for an in-person interview for the 2020 Pediatric Surgery Match were offered the Big Five Inventory (BFI) questionnaire. An initial rank order list (ROL) was formulated employing application information and in-person interview score. A reformulated ROL was created after incorporating BFI data. Correlation of specific personality characteristics vs position on the ROL was assessed for both the initial ROL and the reformulated ROL. RESULTS: Thirty-four applicants were interviewed and 24 (70.6%) were ranked. Linear regression analysis identified a lower neuroticism score directly related to a higher position on the initial ROL {R = .4626, P = .023}. The reformulated ROL identified a higher conscientiousness score {R = .5331; P = .007} and a lower neuroticism score {R = -.4383; P = .032} correlated with ascending the final ROL. CONCLUSIONS: The self-administered BFI adds objectivity to personality determination gleamed from the standard face-to-face interview. Conscientiousness and neuroticism had a significant impact on the final ROL position. Adding objective personality data appears to provide additional aid in this difficult process.


Assuntos
Personalidade , Especialidades Cirúrgicas , Criança , Humanos , Inquéritos e Questionários
7.
Int J Surg Case Rep ; 87: 106412, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34560589

RESUMO

INTRODUCTION: Incidentally found congenital pulmonary airway malformations (CPAM) in older children are extremely rare and have traditionally been managed with minimally invasive versus open lobectomy of the affected lobe. PRESENTATION OF CASE: In this report, we present a 11-year-old male who presented with a recurrent spontaneous pneumothorax and was found to have a large symptomatic CPAM confined to a single segment of the right lower lobe. The patient was successfully treated with thoracoscopic segmentectomy without any residual disease seen on follow up imaging. DISCUSSION: Minimally invasive thoracoscopic approach has many advantages over open approach including better pain control, reduced hospital length of stay, and decreased intraoperative blood loss. With increasing use of minimally invasive approaches, lung-sparing surgery has demonstrated to be a viable and an attractive option for definitive resection of CPAM, without compromising resection margins and/or future lung function. CONCLUSION: This report demonstrates that minimally invasive lung-sparing surgical treatment of a large CPAM is feasible in older children.

8.
Am J Case Rep ; 22: e929247, 2021 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-33568621

RESUMO

BACKGROUND Teratoma, a tumor containing a variety of tissues, is a broad diagnosis containing mature teratoma, immature teratoma, and teratomas with malignant transformation. The tumor forms during embryological development secondary to unsuccessful migration of primordial germ cells. A specific type of mature teratoma, containing human-like features, is called a fetiform teratoma. The fetiform teratoma is often compared and confused with fetus in fetu, a reabsorbed twin. While these tumors have commonly been described in the gonads, the retroperitoneal location finding on antenatal imaging is rare. The distinction between the aforementioned subtypes is not well established, proving a challenging diagnosis prior to resection. CASE REPORT We present a case of a newborn male with a prenatal diagnosis of retroperitoneal cystic mass. Although prenatal imaging was obtained, the diagnosis remained unclear. After birth, planned surgical excision on day of life 7 showed the suprarenal mass contained contiguous intestinal elements. Histopathology examination revealed a mature cystic teratoma with multiple tissue types, including colonic, brain, respiratory, lymphatics, and nerves, reminiscent of fetiform teratoma. This case report presents an interesting example of differentiating elements straddling the diagnoses mentioned above. CONCLUSIONS This is the first reported case of fetiform teratoma diagnosed in a newborn and is especially unique for having the element of intestinal duplication within the retroperitoneal mass. The differentiating features of fetus in fetu and fetiform teratoma depend on subjective distinctions. The case provides an opportunity to discuss the differentials and management strategies.


Assuntos
Doenças Peritoneais , Teratoma , Feminino , Feto/diagnóstico por imagem , Humanos , Recém-Nascido , Masculino , Gravidez , Diagnóstico Pré-Natal , Teratoma/diagnóstico por imagem , Gêmeos
9.
J Pediatr Surg ; 53(6): 1085-1086, 2018 06.
Artigo em Inglês | MEDLINE | ID: mdl-29627176
10.
J Surg Res ; 218: 298-305, 2017 10.
Artigo em Inglês | MEDLINE | ID: mdl-28985865

RESUMO

BACKGROUND: The Big Five framework examines five factors that represent a description of human personality. These factors correlate with success measures and job satisfaction. The Big Five Inventory is a 44-item instrument designed to measure the Big Five framework. Our aim was to document the distribution of Big Five personality traits among Pediatric Surgery fellowship applicants, compare with community norms, surgical residents, between genders, and correlate to the fellowship match results. MATERIALS AND METHODS: Forty Pediatric Surgery fellowship applicants at a university hospital completed the Big Five Inventory during the interview process. It was analyzed and compared with general surgery residents' results and community norms. The data were compared regarding gender and match results. Continuous variables were compared by unpaired t-tests and Mann-Whitney tests. A P value <0.05 was considered significant. RESULTS: The 40 applicants were equally divided between male and female. When compared with general surgery residents and community norms, applicants of both genders scored higher on agreeableness (P < 0.01), conscientiousness (P < 0.01), and emotional stability (P < 0.01). Applicants scored higher on openness when compared with surgical residents (P < 0.01). Male applicants scored higher on emotional stability than females (P = 0.026). Matched applicants scored higher for conscientiousness than unmatched applicants (P = 0.016). CONCLUSIONS: Pediatric Surgery fellowship applicants expressed higher levels of desirable professional traits compared with general surgery residents and community norms. Male applicants demonstrated higher emotional stability than females. Conscientiousness was higher in matching applicants. This first reported experience with personality testing in Pediatric Surgery fellow selection demonstrated potential utility in applicant matching.


Assuntos
Testes de Personalidade/estatística & dados numéricos , Feminino , Humanos , Masculino , Pediatria/estatística & dados numéricos , Especialidades Cirúrgicas/estatística & dados numéricos
11.
J Pediatr Surg ; 52(12): 1904-1908, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28947326

RESUMO

AIM: The Pediatric Surgery fellow selection is a multi-layered process which has not included assessment of surgical dexterity. MATERIALS AND METHODS: Data was collected prospectively as part of the 2016 Pediatric Surgery Match interview process. Applicants completed a questionnaire to document laparoscopic experience and fine motor skills activities. Actual laparoscopic skills were assessed using a simulator. Time to complete an intracorporeal knot was tabulated. An initial rank list was formulated based only on the ERAS application and interview scores. The rank list was re-formulated following the laparoscopic assessment. Un-paired T-test and regression were utilized to analyze the data. RESULTS: Forty applicants were interviewed with 18 matched (45%). The mean knot tying time was 201.31s for matched and 202.35s for unmatched applicants. Playing a musical instrument correlated with faster knot tying (p=0.03). No correlation was identified between knot tying time and either video game experience (p=0.4) or passing the FLS exam (p=0.78). Laparoscopic skills assessment lead to significant reordering of rank list (p=0.01). CONCLUSIONS: Laparoscopic skills performance significantly impacted ranking. Playing a musical instrument correlated with faster knot tying. No correlation was identified between laparoscopic performance and passing the FLS exam or other activities traditionally believed to improve technical ability. TYPE OF STUDY: Prospective study. LEVEL OF EVIDENCE: Level II.


Assuntos
Competência Clínica , Bolsas de Estudo , Laparoscopia/educação , Pediatria/educação , Técnicas de Sutura/educação , Adulto , Criança , Humanos , Internato e Residência , Estudos Prospectivos , Técnicas de Sutura/instrumentação
12.
J Pediatr Surg ; 52(6): 1060-1061, 2017 06.
Artigo em Inglês | MEDLINE | ID: mdl-28365102
13.
J Pediatr Surg ; 52(6): 1040-1044, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28389078

RESUMO

BACKGROUND/PURPOSE: Lean Six Sigma (LSS) is a process improvement methodology that utilizes a collaborative team effort to improve performance by systematically identifying root causes of problems. Our objective was to determine whether application of LSS could improve efficiency when applied simultaneously to all services of an academic children's hospital. METHODS: In our tertiary academic medical center, a multidisciplinary committee was formed, and the entire perioperative process was mapped, using fishbone diagrams, Pareto analysis, and other process improvement tools. Results for Children's Hospital scheduled main operating room (OR) cases were analyzed, where the surgical attending followed themselves. RESULTS: Six hundred twelve cases were included in the seven Children's Hospital operating rooms (OR) over a 6-month period. Turnover Time (interval between patient OR departure and arrival of the subsequent patient) decreased from a median 41min in the baseline period to 32min in the intervention period (p<0.0001). Turnaround Time (interval between surgical dressing application and subsequent surgical incision) decreased from a median 81.5min in the baseline period to 71min in the intervention period (p<0.0001). CONCLUSION: These results demonstrate that a coordinated multidisciplinary process improvement redesign can significantly improve efficiency in an academic Children's Hospital without preselecting specific services, removing surgical residents, or incorporating new personnel or technology. STUDY TYPE: Prospective comparative study, Level II.


Assuntos
Centros Médicos Acadêmicos/organização & administração , Eficiência Organizacional/normas , Hospitais Pediátricos/organização & administração , Salas Cirúrgicas/organização & administração , Assistência Perioperatória/normas , Melhoria de Qualidade/organização & administração , Gestão da Qualidade Total , Centros Médicos Acadêmicos/estatística & dados numéricos , California , Criança , Hospitais Pediátricos/estatística & dados numéricos , Humanos , Salas Cirúrgicas/estatística & dados numéricos , Assistência Perioperatória/estatística & dados numéricos , Avaliação de Processos em Cuidados de Saúde , Estudos Prospectivos , Melhoria de Qualidade/estatística & dados numéricos , Fatores de Tempo
14.
J Pediatr Surg ; 52(6): 984-988, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28410786

RESUMO

PURPOSE: The aim was to review the respiratory failure causes and outcomes of infants with omphalocele or gastroschisis receiving ECMO and reported to the Extracorporeal Life Support Organization (ELSO). METHODS: Gastroschisis and omphalocele infants supported with ECMO and reported to the ELSO Registry between 1992 and 2015 were retrospectively reviewed. Clinical variables, diagnosis of respiratory failure (pulmonary hypertension (PHN), congenital heart defects (CHD), congenital diaphragmatic hernia (CDH), and sepsis), and outcomes were recorded. Univariate analysis was performed using Student's t-test for continuous or Fisher's exact test for categorical variables. RESULTS: Fifty-two infants with gastroschisis (41) (79%) or omphalocele (11) (21%) were identified. The survival to discharge rate of 51% for gastroschisis remained stable and was significantly higher (P=0.05). The overall mortality rate for omphalocele was 82%. Omphalocele had significantly more PHN (P<0.01), CDH (P<0.01), and multiple anomalies (P=0.04) had significantly more sepsis (P=0.02), and none had a CDH. CONCLUSION: Infants with gastroschisis requiring ECMO support have significantly better survival than omphaloceles, and respiratory failure is significantly associated with sepsis. The majority of omphalocele infants die despite ECMO, and respiratory failure is associated PHN and CDH. The association of omphalocele, PHN, and CDH merits further investigation. STUDY TYPE AND EVIDENCE LEVEL: Retrospective comparative study of Registry Database, Level 3.


Assuntos
Oxigenação por Membrana Extracorpórea , Gastrosquise/complicações , Hérnia Umbilical/complicações , Insuficiência Respiratória/terapia , Feminino , Gastrosquise/mortalidade , Hérnia Umbilical/mortalidade , Humanos , Lactente , Recém-Nascido , Masculino , Sistema de Registros , Insuficiência Respiratória/etiologia , Insuficiência Respiratória/mortalidade , Estudos Retrospectivos , Taxa de Sobrevida , Resultado do Tratamento
15.
Semin Pediatr Surg ; 22(3): 144-8, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-23870208

RESUMO

The mind-body connection is receiving increasing scrutiny in a large number of clinical settings, although research has lagged in the pediatric specialties. Psychoneuroimmunology (PNI) is a novel interdisciplinary scientific field that examines the relationship of the mind to the patient's neurologic, endocrine, and immune systems by examining critical parameters such as the effects of mental stress on wound healing and infection rates. Techniques that modify a patient's emotional and mental responses to illness and surgery have positive effects on their physiology resulting in improved recoveries and higher patient satisfaction rates. In the appropriate clinical settings, an awareness of PNI can enhance outcomes for pediatric surgical patients.


Assuntos
Terapia Cognitivo-Comportamental/métodos , Terapias Mente-Corpo/psicologia , Psiconeuroimunologia/métodos , Estresse Psicológico/psicologia , Cicatrização/fisiologia , Criança , Humanos , Pediatria/métodos , Especialidades Cirúrgicas/métodos , Estresse Psicológico/terapia
16.
J S C Med Assoc ; 102(1): 17-8, 2006 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-16704182

RESUMO

Burn injuries remain a leading cause of unintentional injuries in children. Improvements in burn care technology, comprehensive outpatient services, and a team approach to care can reduce hospital stays and ease recovery for patients and families. Burn awareness and fire prevention education to the community and to health care providers may further decrease the incidence of pediatric burn injuries. Primary care providers can have a great impact on reducing the opportunity for a burn injury in a child by sharing what is known about burn incidence and prevention with the children and families they serve.


Assuntos
Prevenção de Acidentes , Queimaduras/prevenção & controle , Conhecimentos, Atitudes e Prática em Saúde , Unidades de Queimados , Criança , Hospitais Pediátricos , Humanos , Medição de Risco , Fatores de Risco , South Carolina
18.
J Pediatr Surg ; 38(3): 354-7; discussion 354-7, 2003 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-12632348

RESUMO

BACKGROUND: Injury to the pancreas is rare in pediatric trauma. Identification of pancreatic injury relies on clinical, radiographic, and laboratory data. Serum screening for pancreatic injury frequently is used but has not proven to correlate well with pancreatic injury. This study investigated utility and cost effectiveness of serum assessment of amylase and lipase. METHODS: A retrospective study of 1,821 pediatric trauma patients over 64 months was conducted. A total of 293 (16%) of these patients suffered trauma to the torso 195 (11%) of whom had confirmed intraabdominal injury. Eight pancreatic injuries (4% of abdominal injuries) were identified; 5 underwent surgery for pancreatic ductal injury. One patient not operated on had a pseudocyst that required late drainage. RESULTS: Serum amylase or lipase levels (AMY/LIP) were measured in 507 (28%) patients. A total of 116 (23%) had elevated AMY/LIP levels. Six of 8 with proven pancreatic injury underwent AMY/LIP testing; 5 had elevated values. Forty-eight percent of patients with elevated AMY/LIP levels had no evidence of intraabdominal injury. Seventy-four of 116 (64%) with elevated AMY/LIP levels underwent abdominal and pelvic computed tomography (CT) scanning, yet 38 (51%) of these had completely normal scans. Many patients with elevated AMY/LIP levels (cost, $6 per test) underwent screening CT scans (cost, $592 per test) based on AMY/LIP alone. No patient with elevated AMY/LIP levels but without clinical suspicion was proven to have pancreatic injury. Cost data are presented. CONCLUSIONS: Serum amylase and lipase determinations may support clinical suspicion in the diagnosis of pediatric pancreatic trauma but are not reliable or cost effective as screening tools. Costs incurred from routine serum amylase and lipase or from imaging tests subsequent to elevated serum values may be significant and unjustified.


Assuntos
Amilases/sangue , Lipase/sangue , Pâncreas/lesões , Traumatismos Abdominais/sangue , Traumatismos Abdominais/diagnóstico por imagem , Traumatismos Abdominais/economia , Traumatismos Abdominais/cirurgia , Biomarcadores , Análise Custo-Benefício , Testes Diagnósticos de Rotina/economia , Custos Hospitalares , Humanos , Pâncreas/diagnóstico por imagem , Pâncreas/enzimologia , Pâncreas/cirurgia , Pseudocisto Pancreático/sangue , Pseudocisto Pancreático/cirurgia , Valor Preditivo dos Testes , Estudos Retrospectivos , Tomografia Computadorizada por Raios X/economia
19.
J Pediatr Surg ; 37(3): 539-44, 2002 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-11877684

RESUMO

BACKGROUND/PURPOSE: Despite aggressive treatment with surgery, chemotherapy, and radiotherapy, the prognosis for many children with neuroblastoma remains poor. Targeted toxins represent novel cancer therapeutics designed to selectively target and kill cancer cells. The authors have developed a novel fusion toxin, DT5F11, consisting of truncated diphtheria toxin (DT(A)) linked to a single chain antibody (sc5F11) targeting the GD(2) antigen found on most neuroblastoma cells. This report describes the construction, expression, and in vitro function of DT5F11. METHODS: Utilizing restriction enzyme digestion, polymerase chain reaction amplification, and gel electrophoresis, the prkDTL5F11 plasmid was created by the fusion of distinct coding sequences for a single-chain GD(2) targeting antibody (sc5F11) and truncated diphtheria toxin (DT(A)). DH5alpha Escherichi coli-competent cells were transformed with prkDTL5F11; DNA was amplified, isolated, and sequenced. The fusion protein was expressed and assayed by Western blot. Targeted cytotoxicity was analyzed on GD(2)-positive (SK-N-AS, IMR-32, SK-N-MC, LAN-1) and GD(2)-negative (HeLa) cells. RESULTS: Fluorescent dye-labeled cycle sequencing identified the constructed fusion toxin gene. Western blot analysis using a mouse antihuman DT(A) antibody showed a 69-kD band identifying the fusion toxin, DT5F11. Targeted cell killing with DT5F11 was seen only in GD(2) positive cells. CONCLUSIONS: This study demonstrates creation of a novel fusion toxin with effective GD(2)-targeted cellular toxicity. Further investigation of this fusion toxin as a therapeutic agent in the management of neuroblastoma is warranted.


Assuntos
Citotoxicidade Imunológica , Imunotoxinas/uso terapêutico , Neuroblastoma/imunologia , Neuroblastoma/patologia , Anticorpos Monoclonais/biossíntese , Anticorpos Monoclonais/química , Anticorpos Monoclonais/genética , Anticorpos Monoclonais/uso terapêutico , Anticorpos Antineoplásicos/biossíntese , Anticorpos Antineoplásicos/genética , Anticorpos Antineoplásicos/uso terapêutico , Antígenos de Neoplasias/biossíntese , Antígenos de Neoplasias/imunologia , Morte Celular/efeitos dos fármacos , Morte Celular/imunologia , Toxina Diftérica/biossíntese , Toxina Diftérica/genética , Toxina Diftérica/uso terapêutico , Gangliosídeos/biossíntese , Gangliosídeos/imunologia , Células HeLa , Humanos , Fragmentos Fab das Imunoglobulinas/biossíntese , Fragmentos Fab das Imunoglobulinas/genética , Fragmentos Fab das Imunoglobulinas/uso terapêutico , Imunotoxinas/química , Imunotoxinas/genética , Neuroblastoma/tratamento farmacológico , Fragmentos de Peptídeos/biossíntese , Fragmentos de Peptídeos/genética , Fragmentos de Peptídeos/uso terapêutico , Plasmídeos/genética , Plasmídeos/uso terapêutico , Proteínas Recombinantes de Fusão/biossíntese , Proteínas Recombinantes de Fusão/química , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/uso terapêutico , Células Tumorais Cultivadas
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