RESUMO
The MDMV (Maize Dwarf Mosaic Virus, MDMV) CP (Coat Protein, CP) gene was cloned by RT-PCR method and introduced into the embryonic calli derived from immature embryos of elite inbred 18-599hong and 18-599bai via particle bombardment. Bombarded calli were selected on selection medium containing 5-10 mg/L (PPT) Bialaphos. From resistant calli, 79 plantlets were regenerated. 18 of 79 were grown and harvested. The results of Southern blotting and PCR analysis demonstrated that MDMV CP have been integrated into the genome of the transgenic plants. PCR-positive progeny plants were artificially inoculated with MDMV strain B, and the average chlorosis of the functional leaves of each plant was investigated. The typical symptoms were observed from the leaves of the control inbreds. while, the presence of the MDMV CP gene provided resistance to inoculation with MDMV strain B.
Assuntos
Proteínas do Capsídeo/genética , Vírus do Mosaico/genética , Plantas Geneticamente Modificadas , Zea mays/genética , Zea mays/virologia , Clonagem Molecular , Doenças das Plantas/genética , Doenças das Plantas/virologia , TransfecçãoRESUMO
BACKGROUND: The standard of care of patients with cancer of cardia and esophagus still remains surgery in early stage. One of the most feared complications after such procedure is anastomotic leak. AIM: We present our experience with omental wrapping of anastomosis (omentoplasty) to decrease the anastomotic leak. SETTINGS AND DESIGN: Retrospective study. MATERIALS AND METHODS: An analysis of 50 consecutive patients, who underwent surgical resection for cancer of cardia and esophagus at BPKMCH, is done. For cancer of esophagus, a 10 cm proximal tumor free margin and for lesions of cardia, at least 5 cm margin was achieved. A 5 cm distal tumor free margin was achieved in each case. A subset of patients was considered for omentoplasty after completion of anastomosis. RESULTS: There were 29 male and 21 female with a mean age of 56.3 years. The average postoperative stay was 13.14 days. The stomach was the organ of substitute in 48 and jejunum in 2 cases. Omentoplasty was done in 37 cases, whereas in 13 cases, no omental wrapping was done. The rate of anastomotic leak was 6%. There was no leak from anastomosis placed at chest, whereas three cases of leak was observed in the anastomosis at the level of neck (P=.013). Overall, there was no leak in omentoplasty group, whereas there were three cases with leak in the group without omentoplasty (P=0.003). CONCLUSION: Omentoplasty should be considered in every case after surgical resection for cancer of cardia and esophagus.
Assuntos
Anastomose Cirúrgica/métodos , Neoplasias Esofágicas/cirurgia , Esofagectomia/métodos , Gastrostomia/métodos , Omento/transplante , Neoplasias Gástricas/cirurgia , Adulto , Idoso , Idoso de 80 Anos ou mais , China/epidemiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Complicações Pós-Operatórias/epidemiologia , Complicações Pós-Operatórias/prevenção & controle , Estudos RetrospectivosRESUMO
By using the matured embryos of Japonica rice variety Zhonghua No. 11 as explants, rice transformation was performed by Agrobacterium-mediated co-cultivation method, resulting in 1489 independent transgenic rice plants that carry a T-DNA insertion. Genomic DNA gel-blot and PCR analyses showed that 69.8% of the total lines contain the inserted T-DNA. The flanking sequence of T-DNA in transgenic rice plants was analyzed using Tail-PCR. In addition, we have evaluated 1066 T1 transgenic lines on heading days, plant height and panicles per hill, and found different types of mutants from a number of lines.
Assuntos
DNA Bacteriano/genética , Mutagênese Insercional , Oryza/genética , Reação em Cadeia da PolimeraseRESUMO
Since it was established that the alteration in gene expression occur during cold acclimation, a major goal in cold acclimation research has been to identify cold-responsive genes and to determine whether they play roles in freezing tolerance. Many cold-regulated genes (COR) were isolated and characterized in Arabidopsis and other cold tolerant plant species. Studies on regulation of COR in Arabidopsis have resulted in the discovery of a family of transcriptional activators, of which, CBF1, a member of the gene family, controls expression of a battery of COR in Arabidopsis and other cold tolerant plant species. During recent years, CBF-like genes were found in the genomes of chilling-sensitive plant species such as tomato and maize. Over-expression of Arabidopsis CBF1 confers elevated tolerance to chilling and drought stresses in transgenic tomato. These results promote our effort to identify and characterize CBF-like genes to improve tolerance of chilling-sensitive plant species to chilling and drought stresses.
Assuntos
Aclimatação/genética , Proteínas de Arabidopsis/genética , Arabidopsis/genética , Proteínas de Ligação a DNA/genética , Genes de Plantas , Transativadores/genética , Arabidopsis/metabolismo , Arabidopsis/fisiologia , Proteínas de Arabidopsis/metabolismo , Temperatura Baixa , Proteínas de Ligação a DNA/metabolismo , Desastres , Congelamento , Regulação da Expressão Gênica de Plantas , Solanum lycopersicum/genética , Transativadores/metabolismo , Zea mays/genéticaRESUMO
Starch is the most important source of calories and a vital storage component in plants. The characterization and production of starch variants from mutation and with transgenic technology has improved our understanding of the synthesis of starch granule. In starch biosynthesis in plants, four enzymes, including ADP-glucose pyrophosphorylase, starch synthase, starch branching enzyme and starch debranching enzyme, are widely accepted from an enormous amount of research aimed primarily at enzyme characterization. As many genes encoding the enzymes and their multiple isoforms in starch biosynthesis pathway have been isolated, genetic manipulation of the starch biosynthesis pathway shows to be a practical way by which starch quantity is increased and starch with novel properties can be created.