RESUMO
7Methoxyheptaphylline (7MH) is a carbazole extracted from Clausena harmandiana, a medicinal plant that is used to treat headaches and stomachaches. The aim of the present study was to examine the neuroprotective effects and anticancer activity of 7MH. Cell death was assessed using an MTT assay and flow cytometry. The expression of apoptosisrelated proteins was determined by western blot analysis. An animal model was used to test antimetastasis. The interactions between 7MH and the molecular target were observed using molecular docking. The results revealed that 7MH provided protection against hydrogen peroxide (H2O2)induced neuronal cell death. In cancer cells, 7MH induced SHSY5Y, 4T1, HT29, HepG2, and LNCaP cell death. 7MH inhibited metastasis of HT29 cells in vitro and 4T1Luc cells in vitro and in vivo. 7MH inhibited proteins, including Pglycogen synthase kinase (GSK)3, and cleaved caspase3, but it activated antiapoptotic proteins in H2O2induced SHSY5Y cell death. By contrast, 7MH activated the cleaving of caspase3 and GSK3, but it suppressed antiapoptotic proteins in SHSY5Y cells. 7MH reduced the levels of NFκB and STAT3 in 4T1 cells; phosphop65, Erk, and MAPK13 in LNCaP cells; and phosphoErk and matrix metalloproteinase9 in HT29 cells. Molecular docking analysis showed that 7MH targets TAK1 kinase. The present study indicated that 7MH induced apoptosis of cancer cells and provided protection against H2O2induced neuron cell death via TAK1 kinase.
