RESUMO
The interaction of 7 monoclonal antibodies (MCA) to the nucleocapsid complex and 3 MCA to protein G of the vaccine virus Vnukovo-32 with 33 and 27 members of the rabies virus group, respectively, was studied. The indirect immunofluorescence test showed 7 MCA to the nucleocapsid complex to recognize 4 antigenic determinants (AD). Two MCA recognized the AD common for all the viruses under study. Individual ecological variants may be detected using other MCA. Three MCA to protein G had marked virus-neutralizing activity. MCA 1C5 neutralized all the rabies viruses under study. The other two MCA differed in the number of viruses they could neutralize. The viruses under study were divided into 4 groups depending on their interactions with certain MCA in neutralization test.
Assuntos
Anticorpos Monoclonais/imunologia , Reações Antígeno-Anticorpo/imunologia , Vacina Antirrábica/imunologia , Vírus da Raiva/imunologia , Proteínas Estruturais Virais/imunologia , Animais , Anticorpos Monoclonais/análise , Anticorpos Monoclonais/isolamento & purificação , Antígenos Virais/imunologia , Imunofluorescência , Hibridomas/imunologia , Masculino , Camundongos , Camundongos Endogâmicos CBA , Testes de Neutralização/métodos , Inoculações SeriadasRESUMO
Yuli virus was isolated by intracerebral (i.c.) inoculation of suckling mice with a 10% brain suspension from 11-year-old patient who died under signs of atypical hydrophobia after a bat bite into lower lip. Identification with a panel of monoclonal antibodies (MoAb) to nucleocapsid protein (NP) confirmed that Yuli virus belongs to Lyssavirus genus, as an antigenic variant of the European Duvenhage virus.
Assuntos
Anticorpos Monoclonais/imunologia , Vírus da Raiva/isolamento & purificação , Raiva/microbiologia , Animais , Mordeduras e Picadas , Encéfalo/microbiologia , Encéfalo/patologia , Capsídeo/imunologia , Criança , Quirópteros , Cricetinae , Encefalite/complicações , Encefalite/diagnóstico , Humanos , Lábio/microbiologia , Camundongos , Raiva/complicações , Vírus da Raiva/imunologia , Proteínas do Core Viral/imunologiaAssuntos
Vírus da Raiva/isolamento & purificação , Raiva/diagnóstico , Animais , Neoplasias dos Nervos Cranianos , Estudos de Avaliação como Assunto , Humanos , Camundongos , Neurilemoma , Ratos , Fatores de Tempo , Gânglio Trigeminal , Células Tumorais Cultivadas , Virologia/métodos , Cultura de Vírus/métodosRESUMO
The L-28 strain of street rabies virus isolated from the salivary gland of a red fox caused chronic infection in mice. After intracerebral (i.e.) infection with virus-containing fox salivary gland suspension, 13.9% of mice showed clinical signs of disease for 11--52 days. Clear-cut stages of irritation and paralysis were distinguished in the course of infection of mice. The diagnosis of chronic rabies in mice was confirmed by immunofluorescence and isolation of virus by i.c. inoculation of mice. Electron microscopic examination of the Ammon's horn of mice revealed a slow development of morphological changes connected with the formation of virus-specific structures. The gradual increase in the number and size of aggregates of viral ribonucleoprotein was followed by the appearance of membranous and tubular structures. Along with the latter, typical bullet-shaped particles of rabies virus were found in some neurons on the 43rd day of infection.